scholarly journals Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses

2010 ◽  
Vol 60 (10) ◽  
pp. 2298-2302 ◽  
Author(s):  
Masayuki Miyazaki ◽  
Yuriko Nagano ◽  
Yoshihiro Fujiwara ◽  
Yuji Hatada ◽  
Yuichi Nogi
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Sperm Whale ◽  
Gliding Motility ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Dna Relatedness ◽  
Definition Of ◽  
Type Strains

A Gram-negative, rod-shaped, non-spore-forming, strictly aerobic strain with gliding motility, designated JAMB N27T, was isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan, at a depth of 219 m. Strain JAMB N27T contained MK-6 as the major isoprenoid quinone and iso-C15 : 0, iso-C15 : 1, C16 : 1 and iso-C17 : 1 as the predominant fatty acids. Casein, chitin, gelatin and starch were degraded. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JAMB N27T represented a separate lineage within the genus Aquimarina. The DNA G+C content of strain JAMB N27T was 33.1 mol%. DNA–DNA relatedness values between strain JAMB N27T and type strains of species of the genus Aquimarina were significantly lower than the cut-off value accepted for the definition of a novel species. Therefore, strain JAMB N27T represents a novel species, for which the name Aquimarina macrocephali sp. nov. is proposed. The type strain is JAMB N27T (=JCM 15542T=NCIMB 14508T).

scholarly journals Pseudovibrio japonicus sp. nov., isolated from coastal seawater in Japan

2007 ◽  
Vol 57 (9) ◽  
pp. 1952-1955 ◽  
Author(s):  
Shoichi Hosoya ◽  
Akira Yokota
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Biochemical Characteristics ◽  
Boso Peninsula ◽  
Coastal Seawater ◽  
Definition Of ◽  
Type Strains

A Gram-negative, motile, rod-shaped bacterium (WSF2T) was isolated from coastal seawater of the Boso Peninsula in Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain WSF2T represented a separate lineage within the genus Pseudovibrio. The DNA G+C content of strain WSF2T was 51.7 mol%. DNA–DNA hybridization values between strain WSF2T and the type strains of Pseudovibrio species were significantly lower than those accepted as the phylogenetic definition of a species. Furthermore, some biochemical characteristics indicated that strain WSF2T differed from other Pseudovibrio species. Based on these characteristics, it is proposed that the isolate represents a novel species, Pseudovibrio japonicus sp. nov. The type strain is WSF2T (=IAM 15442T=NCIMB 14279T=KCTC 12861T).

scholarly journals Isoptericola chiayiensis sp. nov., isolated from mangrove soil

2011 ◽  
Vol 61 (7) ◽  
pp. 1667-1670 ◽  
Author(s):  
M. Tseng ◽  
H. C. Liao ◽  
W. P. Chiang ◽  
G. F. Yuan
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Mangrove Soil ◽  
Low Levels ◽  
Type Strains

A novel actinomycete, designated strain 06182M-1T, was isolated from a mangrove soil sample collected from Chiayi County in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences revealed levels of similarity of 97.0–98.8 % to the type strains of recognized species of the genus Isoptericola. Chemotaxonomic data also supported the placement of strain 06182M-1T within the genus Isoptericola. However, the low levels of DNA–DNA relatedness between the novel strain and the type strains of recognized species of the genus Isoptericola, in combination with differential phenotypic data, demonstrate that strain 06182M-1T represents a novel species of the genus Isoptericola, for which the name Isoptericola chiayiensis sp. nov. is proposed. The type strain is 06182M-1T ( = BCRC 16888T  = KCTC 19740T).

scholarly journals Knoellia flava sp. nov., isolated from pig manure

2012 ◽  
Vol 62 (2) ◽  
pp. 384-389 ◽  
Author(s):  
Xiang Yu ◽  
Yan Du ◽  
Gejiao Wang
Keyword(s):  
Fatty Acids ◽  
Genomic Dna ◽  
Type Strain ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Pig Manure ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Dna Relatedness ◽  
Type Strains

A Gram-positive, aerobic, non-spore-forming actinobacterial strain, designated strain TL1T, was isolated from pig manure in Wuhan, China. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol. The predominant menaquinone was MK-8(H4). The major fatty acids (>10 %) were iso-C16 : 0, iso-C15 : 0 and C17 : 1ω8c. The genomic DNA G+C content was 70.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TL1T was most closely related to the type strains of Knoellia sinensis (98.5 %), Knoellia subterranea (98.2 %) and Knoellia aerolata (96.9 %). DNA–DNA relatedness values of strain TL1T with the type strains of K. sinensis and K. subterranea were 27.3 and 34.0 %, respectively. Comparison of phenotypic, chemotaxonomic and phylogenetic characteristics among strain TL1T and related organisms revealed that the isolate represents a novel species of the genus Knoellia, for which the name Knoellia flava sp. nov. is proposed; the type strain is TL1T ( = CGMCC 1.10749T = KCTC 19810T).

scholarly journals Maribacter confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3079-3085 ◽  
Author(s):  
Sooyeon Park ◽  
Yong-Taek Jung ◽  
Ji-Min Park ◽  
Sung-Min Won ◽  
Jung-Hoon Yoon
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Dna Relatedness ◽  
Freshwater Spring ◽  
Type Strains ◽  
Sequence Similarities

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, SSK2-2T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK2-2T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain SSK2-2T fell within the clade comprising the type strains of species of the genus Maribacter, joining the type strain of Maribacter sedimenticola with which it shared 99.4 % similarity. Sequence similarities to the type strains of other Maribacter species were 94.6–98.2 %. Strain SSK2-2T contained MK-6 as the predominant menaquinone and iso-C17 : 0 3-OH, iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0) as the major fatty acids. The major polar lipids of strain SSK2-2T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-2T was 38.2 mol% and mean levels of DNA–DNA relatedness with the type strains of four phylogenetically related species of the genus Maribacter were 11–24 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain SSK2-2T is separate from other Maribacter species. On the basis of the data presented, strain SSK2-2T is considered to represent a novel species of the genus Maribacter, for which the name Maribacter confluentis sp. nov. is proposed. The type strain is SSK2-2T ( = KCTC 42604T = CECT 8869T).

scholarly journals Streptomyces hyderabadensis sp. nov., an actinomycete isolated from soil

2011 ◽  
Vol 61 (1) ◽  
pp. 76-80 ◽  
Author(s):  
T. V. K. Reddy ◽  
Shaik Mahmood ◽  
Laskaris Paris ◽  
Y. Harish Kumar Reddy ◽  
E. M. H. Wellington ◽  
...  
Keyword(s):  
Type Strain ◽  
Andhra Pradesh ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Streptomyces Olivaceus ◽  
Type Strains

A novel actinomycete, designated strain OU-40T, was isolated from farm soil collected from the Hyderabad region of Andhra Pradesh, southern India. The strain was found to have morphological and chemotaxonomic characteristics typical of species of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain OU-40T belonged to the genus Streptomyces, and was related most closely to Streptomyces pactum NBRC 13433T (99.0 % sequence similarity), Streptomyces olivaceus NBRC 12805T (99.0 %) and Streptomyces parvulus NBRC 13193T (98.8 %). Strain OU-40T could be distinguished from the type strains of its closest phylogenetic relatives based on levels of DNA–DNA relatedness and comparison of morphological and phenotypic data. It is therefore concluded that strain OU-40T represents a novel species of the genus Streptomyces, for which the name Streptomyces hyderabadensis sp. nov. is proposed. The type strain is OU-40T (=CCTCC AA 209024T =PCM 2692T).

scholarly journals Streptomyces sanyensis sp. nov., isolated from mangrove sediment

2011 ◽  
Vol 61 (7) ◽  
pp. 1632-1637 ◽  
Author(s):  
Jin-Lei Sui ◽  
Xiao-Xiong Xu ◽  
Zhi Qu ◽  
Hai-Long Wang ◽  
Hai-Peng Lin ◽  
...  
Keyword(s):  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Aerial Mycelium ◽  
Mangrove Sediment ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Type Strains

A novel strain, 219820T, whose metabolites were found to be active against tumour cells, was isolated and characterized. The isolate belonged to the genus Streptomyces and had white to grey aerial mycelium and long chains of smooth spores in the aerial mycelium. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 219820T had highest similarity to members of the genus Streptomyces and was most closely, albeit loosely, associated with Streptomyces crystallinus NBRC 15401T (98.624 % similarity), Streptomyces melanogenes NBRC 12890T (98.565 %) and Streptomyces noboritoensis NBRC 13065T (98.564 %). However, DNA–DNA relatedness and phenotypic data readily distinguished strain 219820T from these phylogenetically related type strains. It is evident from the combination of genotypic and phenotypic data that strain 219820T represents a novel species of the genus Streptomyces, for which the name Streptomyces sanyensis sp. nov. is proposed; the type strain is 219820T ( = CGMCC 4.5626T  = DSM 42014T).

scholarly journals Streptomyces osmaniensis sp. nov., isolated from soil

2010 ◽  
Vol 60 (8) ◽  
pp. 1755-1759 ◽  
Author(s):  
T. V. K. Reddy ◽  
Shaik Mahmood ◽  
M. Mohammed Idris ◽  
Slawomir Ciesielski
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Novel Species ◽  
Garden Soil ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
The Mean ◽  
Type Strains

A novel actinomycete, designated strain OU-63T, was isolated from garden soil collected on the campus of Osmania University in Hyderabad, southern India. The strain was found to have morphological and chemotaxonomic characteristics typical of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus Streptomyces, and was related most closely to Streptomyces chartreusis NBRC 12753T (98.6 % similarity). However, the mean level of DNA–DNA relatedness between the two strains was only 25±1.73 %. Based on DNA–DNA relatedness, morphological and phenotypic data, strain OU-63T could be distinguished from the type strains of phylogenetically related species. It is therefore considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces osmaniensis sp. nov. is proposed. The type strain is OU-63T (=CCTCC AA209025T =PCM 2690T).

scholarly journals Skermanella aerolata sp. nov., isolated from air, and emended description of the genus Skermanella

2007 ◽  
Vol 57 (7) ◽  
pp. 1539-1542 ◽  
Author(s):  
Hang-Yeon Weon ◽  
Byung-Yong Kim ◽  
Seung-Beom Hong ◽  
Jae-Ho Joa ◽  
Sang-Sik Nam ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Type Strain ◽  
Novel Species ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Emended Description ◽  
Air Sample ◽  
Sequence Similarities

A light-pink-coloured bacterium, designated strain 5416T-32T, was isolated from an air sample in Korea. Cells of the strain were strictly aerobic, Gram-negative, motile (single polar or subpolar flagellum) and rod-shaped. Optimal growth occurred at 25–30 °C and at pH 6.0–7.0. The major quinones were Q-10 and Q-8. The major fatty acids were C18 : 1 ω7c (53.8 %) and C16 : 0 (15.9 %). The G+C content of the genomic DNA was 65.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 5416T-32T was most closely related to Skermanella parooensis, with a similarity of 96.2 %, but relatively low sequence similarities (<92 %) were found with respect to other species with validly published names held in GenBank. Phenotypic and genotypic analyses indicated that strain 5416T-32T could not be assigned to any recognized species. Therefore strain 5416T-32T represents a novel species of the genus Skermanella, for which the name Skermanella aerolata sp. nov. is proposed. The type strain is 5416T-32T (=KACC 11604T=DSM 18479T).

scholarly journals Adhaeribacter aerophilus sp. nov., Adhaeribacter aerolatus sp. nov. and Segetibacter aerophilus sp. nov., isolated from air samples

2010 ◽  
Vol 60 (10) ◽  
pp. 2424-2429 ◽  
Author(s):  
Hang-Yeon Weon ◽  
Soon-Wo Kwon ◽  
Jung-A Son ◽  
Soo-Jin Kim ◽  
Yi-Seul Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Novel Species ◽  
Fatty Acid Content ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Air Samples ◽  
Type Strains ◽  
Sequence Similarities

Three bacterial isolates from air samples in Korea, designated strains 6424S-25T, 6515J-31T and 6424S-61T, were characterized using a polyphasic approach. The cells were strictly aerobic, Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis of their 16S rRNA gene sequences revealed a clear affiliation with the phylum Bacteroidetes. Strains 6424S-25T and 6515J-31T showed 16S rRNA gene sequence similarities of 92.7–94.8 % to type strains of recognized species of the genus Adhaeribacter and strain 6424S-61T was closely related to Segetibacter koreensis Gsoil 664T (93.9 % similarity). The G+C contents of the DNA of strains 6424S-25T, 6515J-31T and 6424S-61T were 44.5, 43.9 and 38.4 mol%, respectively. Major fatty acids of strains 6424S-25T and 6515J-31T were summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B), iso-C15 : 0 and C16 : 1 ω5c. The fatty acid content of strain 6424S-61T mainly comprised iso-C15 : 1 G and iso-C15 : 0. Comparative analysis of phenotypic and phylogenetic traits indicated that strains 6424S-25T and 6515J-31T represented two novel species of the genus Adhaeribacter and that strain 6424S-61T should be considered as a novel species of the genus Segetibacter. The names Adhaeribacter aerophilus sp. nov. (type strain 6424S-25T =KACC 14118T =NBRC 106134T), Adhaeribacter aerolatus sp. nov. (type strain 6515J-31T =KACC 14117T =NBRC 106133T) and Segetibacter aerophilus sp. nov. (type strain 6424S-61T =KACC 14119T =NBRC 106135T) are proposed for these organisms.

scholarly journals Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum

2005 ◽  
Vol 55 (3) ◽  
pp. 1305-1309 ◽  
Author(s):  
Raúl Rivas ◽  
Carmen Gutiérrez ◽  
Adriana Abril ◽  
Pedro F. Mateos ◽  
Eustoquio Martínez-Molina ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
Dna Hybridization ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Gas Production ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Bacterial Strains ◽  
16S Rrna Gene Sequences

Two sporulating bacterial strains designated CECAP06T and CECAP16 were isolated from the rhizosphere of the legume Cicer arietinum in Argentina. Almost-complete 16S rRNA gene sequences identified the isolates as a Paenibacillus species. It was most closely related to Paenibacillus cineris LMG 18439T (99·6 % sequence similarity), Paenibacillus favisporus LMG 20987T (99·4 % sequence similarity) and Paenibacillus azoreducens DSM 13822T (97·7 % sequence similarity). The cells of this novel species were motile, sporulating, rod-shaped, Gram-positive and strictly aerobic. The predominant fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The DNA G+C content of strains CECAP06T and CECAP16 was 51·3 and 50·9 mol%, respectively. Growth was observed from many carbohydrates, but gas production was not observed from glucose. Catalase and oxidase activities were present. The isolates produced β-galactosidase and hydrolysed aesculin. Gelatinase, caseinase and urease were not produced. The results of DNA–DNA hybridization showed that the strains from this study constitute a novel species of the genus Paenibacillus, for which the name Paenibacillus rhizosphaerae sp. nov. is proposed. The type strain is CECAP06T (=LMG 21955T=CECT 5831T).

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