scholarly journals Alteromonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Alteromonas

2004 ◽  
Vol 54 (4) ◽  
pp. 1157-1163 ◽  
Author(s):  
Stefanie Van Trappen ◽  
Tjhing-Lok Tan ◽  
Jifang Yang ◽  
Joris Mergaert ◽  
Jean Swings
Keyword(s):  
Fatty Acid ◽  
Sea Water ◽  
Novel Species ◽  
Sequence Similarity ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Emended Description ◽  
Mother And Daughter ◽  
Taxonomic Approach

Seven novel, cold-adapted, strictly aerobic, facultatively oligotrophic strains, isolated from Antarctic sea water, were investigated by using a polyphasic taxonomic approach. The isolates were Gram-negative, chemoheterotrophic, motile, rod-shaped cells that were psychrotolerant and moderately halophilic. Buds were produced on mother and daughter cells and on prosthecae. Prostheca formation was peritrichous and prosthecae could be branched. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the γ-Proteobacteria and are related to the genus Alteromonas, with 98·3 % sequence similarity to Alteromonas macleodii and 98·0 % to Alteromonas marina, their nearest phylogenetic neighbours. Whole-cell fatty acid profiles of the isolates were very similar and included C16 : 0, C16 : 1 ω7c, C17 : 1 ω8c and C18 : 1 ω8c as the major fatty acid components. These results support the affiliation of these isolates to the genus Alteromonas. DNA–DNA hybridization results and differences in phenotypic characteristics show that the strains represent a novel species with a DNA G+C content of 43–45 mol%. The name Alteromonas stellipolaris sp. nov. is proposed for this novel species; the type strain is ANT 69aT (=LMG 21861T=DSM 15691T). An emended description of the genus Alteromonas is given.

scholarly journals Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola

2004 ◽  
Vol 54 (5) ◽  
pp. 1765-1771 ◽  
Author(s):  
Stefanie Van Trappen ◽  
Tjhing-Lok Tan ◽  
Jifang Yang ◽  
Joris Mergaert ◽  
Jean Swings
Keyword(s):  
Fatty Acid ◽  
Sea Water ◽  
Novel Species ◽  
Sequence Similarity ◽  
The Other ◽  
The Arctic ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Phenotypic Properties ◽  
Emended Description

Four strains of cold-adapted, strictly aerobic and facultative oligotrophic bacteria were isolated from polar seas and investigated using a polyphasic taxonomic approach. Two strains (LMG 21857T and LMG 21854) derive from Arctic sea water whereas the other two strains (LMG 21855 and LMG 21858) were isolated from Antarctic sea water. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the γ-subclass of the Proteobacteria and are related to the genus Glaciecola, with 98·0–99·7 % sequence similarity to Glaciecola mesophila and 94·2–95·3 % sequence similarity to Glaciecola punicea, their nearest phylogenetic neighbours. Two strains (LMG 21855 and LMG 21858) were identified as G. mesophila, whereas DNA–DNA hybridization results and differences in phenotypic characteristics showed that the other two strains (LMG 21857T and LMG 21854) constitute a novel species within the genus Glaciecola, with a DNA G+C content of 44·0 mol%. The isolates are Gram-negative, chemoheterotrophic, motile, rod-shaped cells that are psychrotolerant and moderately halophilic. Buds can be produced on mother cells and on prosthecae. Branch formation of prosthecae occurs. Whole-cell fatty acid profiles of the isolates are very similar and include C16 : 0 and C16 : 1 ω7c as the major fatty acid components. On the basis of genotypic and phenotypic properties, a novel species of the genus Glaciecola is described, for which the name Glaciecola polaris sp. nov. is proposed, with isolate LMG 21857T (=CIP 108324T=ARK 150T) as the type strain. An emended description of the genus Glaciecola is presented.

scholarly journals Alcaligenes aquatilis sp. nov., a novel bacterium from sediments of the Weser Estuary, Germany, and a salt marsh on Shem Creek in Charleston Harbor, USA

2005 ◽  
Vol 55 (6) ◽  
pp. 2571-2575 ◽  
Author(s):  
Stefanie Van Trappen ◽  
Tjhing-Lok Tan ◽  
Emly Samyn ◽  
Peter Vandamme
Keyword(s):  
Fatty Acid ◽  
Dimethyl Sulfide ◽  
Novel Species ◽  
Sequence Similarity ◽  
Alcaligenes Faecalis ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Weser Estuary ◽  
Taxonomic Approach

Four nitrite-dissimilating strains, isolated from Weser Estuary sediments, were investigated using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the ‘Betaproteobacteria’ and are related to the genus Alcaligenes. The highest level of sequence similarity (100 %) was found with strain M3A (=ATCC 700596), a dimethyl sulfide-producing marine isolate that was included in this study. DNA–DNA hybridizations between the five strains and related Alcaligenes faecalis strains confirmed that the former belong to a single and novel species within the genus Alcaligenes. The isolates are Gram-negative, motile, rod-shaped cells with a DNA G+C content of about 56 mol%. The whole-cell fatty acid profiles of the isolates were very similar and included C16 : 0, C17 : 0 cyclo, C18 : 1 ω7c, summed feature 2 (comprising any combination of C12 : 0 aldehyde, an unknown fatty acid of equivalent chain length 10·928, C16 : 1 iso I and C14 : 0 3-OH) and summed feature 3 (C15 : 0 iso 2-OH and/or C16 : 1 ω7c) as the major fatty acid components. On the basis of their phylogenetic, genomic and phenotypic properties, the five novel strains can be assigned to the genus Alcaligenes as a novel species, for which the name Alcaligenes aquatilis sp. nov. is proposed. The type strain is LMG 22996T (=CCUG 50924T).

scholarly journals Description of Sulfitobacter donghicola sp. nov., isolated from seawater of the East Sea in Korea, transfer of Staleya guttiformis Labrenz et al. 2000 to the genus Sulfitobacter as Sulfitobacter guttiformis comb. nov. and emended description of the genus Sulfitobacter

2007 ◽  
Vol 57 (8) ◽  
pp. 1788-1792 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Mi-Hwa Lee ◽  
Tae-Kwang Oh
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Major Fatty Acid ◽  
East Sea ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Unidentified Phospholipid ◽  
Emended Description

A Gram-negative, non-motile and rod-, oval- or coccoid-shaped bacterial strain, DSW-25T, which is phylogenetically closely related to the genera Staleya and Sulfitobacter, was isolated from seawater of the East Sea, Korea, and subjected to a polyphasic taxonomic study. Strain DSW-25T grew optimally at pH 7.0–8.0 and at 25 °C. It contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. Major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content was 56.9 mol%. Strain DSW-25T exhibited 16S rRNA gene sequence similarity values of 98.4 % to the type strain of Staleya guttiformis and of 96.6–97.6 % to Sulfitobacter species. There were no distinct phenotypic, particularly chemotaxonomic, properties to differentiate Staleya guttiformis and strain DSW-25T from the genus Sulfitobacter. DNA–DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain DSW-25T differs from recognized Sulfitobacter species and Staleya guttiformis. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain DSW-25T was classified in the genus Sulfitobacter as a member of a novel species, for which the name Sulfitobacter donghicola sp. nov. is proposed. The type strain is strain DSW-25T (=KCTC 12864T =JCM 14565T). It is also proposed that Staleya guttiformis be transferred to the genus Sulfitobacter as Sulfitobacter guttiformis comb. nov.

scholarly journals Psychrobacter aestuarii sp. nov., isolated from a tidal flat sediment

2010 ◽  
Vol 60 (7) ◽  
pp. 1631-1636 ◽  
Author(s):  
Keun Sik Baik ◽  
Seong Chan Park ◽  
Chae Hong Lim ◽  
Kang Hyun Lee ◽  
Doo Young Jeon ◽  
...  
Keyword(s):  
Tidal Flat ◽  
Novel Species ◽  
Sequence Similarity ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Tidal Flat Sediment ◽  
South Sea ◽  
Distinct Branch

A Gram-negative-staining, non-motile, non-spore-forming and strictly aerobic bacterial strain, SC35T, was isolated from tidal flat sediment collected from the South Sea, Korea, and subjected to a taxonomic study using a polyphasic approach. The organism grew optimally at 20–30 °C and with 1–2 % (w/v) NaCl. Strain SC35T contained ubiquinone-8 as the predominant respiratory lipoquinone and C18 : 1 ω9c as the major fatty acid. The DNA G+C content was 48.5 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain SC35T formed a lineage within the genus Psychrobacter (94.3–96.5 % sequence similarity), forming a distinct branch in a clade also containing Psychrobacter pacificensis NIBH P2K6T and Psychrobacter celer SW-238T. On the basis of phenotypic and phylogenetic data, strain SC35T (=KCTC 22503T=JCM 16343T) was placed in the genus Psychrobacter as the type strain of a novel species, for which the name Psychrobacter aestuarii sp. nov. is proposed.

scholarly journals Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia

2007 ◽  
Vol 57 (7) ◽  
pp. 1424-1428 ◽  
Author(s):  
Wen-Jun Li ◽  
Ping Xu ◽  
Peter Schumann ◽  
Yu-Qin Zhang ◽  
Rüdiger Pukall ◽  
...  
Keyword(s):  
Forest Soil ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Genotypic Differences ◽  
Emended Description ◽  
Diamino Acid ◽  
Chemotaxonomic Markers ◽  
Taxonomic Approach

A Gram-positive, motile, short-rod-shaped strain, designated YIM 004T, was isolated from a forest-soil sample collected from Lijiang, Yunnan Province, China, and was investigated using a polyphasic taxonomic approach. The isolate contained chemotaxonomic markers that corresponded to those of its phylogenetic neighbour, Georgenia muralis, i.e. it possessed peptidoglycan type A4α with lysine as the diagnostic cell-wall diamino acid, the predominant menaquinone was MK-8(H4) and the major fatty acid was ai-C15 : 0. The G+C content of the genomic DNA was 72.9 mol%. Strain YIM 004T exhibited a 16S rRNA gene sequence similarity of 97.3 % and a DNA–DNA relatedness value of 18 % with respect to G. muralis DSM 14418T. On the basis of the phenotypic and genotypic differences between the isolate and G. muralis, strain YIM 004T represents a novel species of the genus Georgenia, for which the name Georgenia ruanii sp. nov. is proposed. The type strain is YIM 004T (=CCTCC AB 204065T=DSM 17458T=KCTC 19029T). In addition, an emended description of the genus Georgenia is presented.

scholarly journals Nereida ignava gen. nov., sp. nov., a novel aerobic marine α-proteobacterium that is closely related to uncultured Prionitis (alga) gall symbionts

2005 ◽  
Vol 55 (2) ◽  
pp. 631-636 ◽  
Author(s):  
M. J. Pujalte ◽  
M. C. Macián ◽  
D. R. Arahal ◽  
W. Ludwig ◽  
K. H. Schleifer ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Sea Water ◽  
Sequence Similarity ◽  
Carbon Sources ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Phylogenetic Distance ◽  
Novel Genus And Species

A Gram-negative, slightly halophilic, non-pigmented, strictly aerobic, chemo-organotrophic bacterium was isolated from Mediterranean sea water off the Spanish coast near Valencia. This strain was poorly reactive, being unable to grow in most carbon sources analysed in minimal medium. However, good growth was observed when more complex media and longer incubation times were used. Phylogenetic analysis based on an almost complete 16S rRNA gene sequence placed strain 2SM4T within the Roseobacter group, in the vicinity of uncultured bacteria described as gall symbionts of several species of the red alga Prionitis. Sequence similarity values between strain 2SM4T and the closest neighbouring species were below 95·0 %. The cellular fatty acid composition of the Mediterranean strain confirmed its position within the ‘Alphaproteobacteria’, sharing 18 : 1ω7c as the major cellular fatty acid. The phylogenetic distance from any taxon with a validly published name and also a number of distinguishing features support the designation of strain 2SM4T as representing a novel genus and species, for which the name Nereida ignava gen. nov., sp. nov. is proposed. The type strain is 2SM4T (=CECT 5292T=DSM 16309T=CIP 108404T=CCUG 49433T).

scholarly journals Salegentibacter chungangensis sp. nov., isolated from a sea sand and emended description of the genus Salegentibacter

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1514-1519 ◽  
Author(s):  
Chatuphon Siamphan ◽  
Wonyong Kim
Keyword(s):  
Sequence Similarity ◽  
Major Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Link Type ◽  
Marine Sand ◽  
Emended Description

A Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterial strain, designated CAU 1289T, was isolated from a marine sand and its taxonomic position was investigated using a polyphasic approach. It grew optimally at pH 6.5 and 30 °C and in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1289T belonged to the genus Salegentibacter , exhibiting sequence similarity values of 93.6–96.3 % to members of this genus, and was related most closely to Salegentibacter mishustinae KMM 6049T (96.3 % similarity). Strain CAU 1289T contained MK-6 as the predominant menaquinone. The major fatty acid was iso-C15 : 0. The cell-wall peptidoglycan of strain CAU 1289T contained meso-diaminopimelic acid. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine, an unidentified phospholipid, an unidentified aminolipid, an unidentified aminophospholipid, an unidentified glycolipid and ten unidentified lipids. The DNA G+C content was 38.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic inference, strain CAU 1289T is considered to represent a novel species of the genus Salegentibacter , for which the name Salegentibacter chungangensis sp. nov. is proposed. The type strain is CAU 1289T (KCTC 24000T = CCUG 64793T). An emended description of the genus Salegentibacter is also proposed.

Brevibacillus fluminis sp. nov., isolated from sediment of estuarine wetland

2010 ◽  
Vol 60 (7) ◽  
pp. 1595-1599 ◽  
Author(s):  
Mi-Jin Choi ◽  
Jee-Yeon Bae ◽  
Ki-Yeon Kim ◽  
Hojeong Kang ◽  
Chang-Jun Cha
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Enrichment Culture ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Estuarine Wetland ◽  
Gene Sequence Analysis ◽  
Taxonomic Approach

Identification of a bacterial strain, designated CJ71T, was carried out using a polyphasic taxonomic approach. Strain CJ71T was isolated from sediment from the estuarine wetland of the Han River, South Korea, by enrichment culture using pyrene as the sole carbon and energy source. The isolate was white-pigmented, rod-shaped, Gram-positive, strictly aerobic and motile. 16S rRNA gene sequence analysis revealed that strain CJ71T had the highest sequence similarity (96.9 %) to Brevibacillus formosus DSM 9885T. The predominant cellular fatty acids in strain CJ71T were anteiso-C15 : 0 (49.5 %), iso-C15 : 0 (16.9 %), iso-C14 : 0 (16.9 %) and iso-C16 : 0 (4.9 %). The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 52.4 mol%. Results from the polyphasic taxonomic study suggest that strain CJ71T represents a novel species of the genus Brevibacillus for which the name Brevibacillus fluminis sp. nov. is proposed; the type strain is CJ71T (=KACC 13381T=JCM 15716T)

scholarly journals Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea

2007 ◽  
Vol 57 (3) ◽  
pp. 548-551 ◽  
Author(s):  
Hang-Yeon Weon ◽  
Byung-Yong Kim ◽  
Min-Kyeong Kim ◽  
Seung-Hee Yoo ◽  
Soon-Wo Kwon ◽  
...  
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Bacterial Strains ◽  
Lysobacter Enzymogenes ◽  
Gene Sequence Analysis ◽  
Greenhouse Soils

Two bacterial strains, designated GH34-4T and GH41-7T, were isolated from greenhouse soil cultivated with cucumber. The bacteria were strictly aerobic, Gram-negative, rod-shaped and oxidase- and catalase-positive. 16S rRNA gene sequence analysis indicated that these strains belong to the genus Lysobacter within the Gammaproteobacteria. Strain GH34-4T showed highest sequence similarity to Lysobacter yangpyeongensis GH19-3T (97.5 %) and Lysobacter koreensis Dae16T (96.4 %), and strain GH41-7T showed highest sequence similarity to Lysobacter antibioticus DSM 2044T (97.5 %), Lysobacter enzymogenes DSM 2043T (97.5 %) and Lysobacter gummosus ATCC 29489T (97.4 %). Levels of DNA–DNA relatedness indicated that strains GH34-4T and GH41-7T represented species clearly different from L. yangpyeongensis, L. antibioticus, L. enzymogenes and L. gummosus. The major cellular fatty acids of strains GH34-4T and GH41-7T were iso-C16 : 0, iso-C15 : 0 and iso-C17 : 1 ω9c, and the major isoprenoid quinone was Q-8. The DNA G+C contents of GH34-4T and GH41-7T were 62.5 and 66.6 mol%, respectively. On the basis of the polyphasic taxonomic data presented, it is evident that each of these strains represents a novel species of the genus Lysobacter, for which the names Lysobacter niabensis sp. nov. (type strain GH34-4T=KACC 11587T=DSM 18244T) and Lysobacter niastensis sp. nov. (type strain GH41-7T=KACC 11588T=DSM 18481T) are proposed.

scholarly journals Niabella tibetensis sp. nov., isolated from soil, and emended description of the genus Niabella

2011 ◽  
Vol 61 (5) ◽  
pp. 1201-1205 ◽  
Author(s):  
Jun Dai ◽  
Fan Jiang ◽  
Yang Wang ◽  
Bo Yu ◽  
Huan Qi ◽  
...  
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
The Family ◽  
Emended Description ◽  
Taxonomic Approach ◽  
Physiological And Biochemical ◽  
Sequence Similarities ◽  
Biochemical Features

Strain 15-4T, a Gram-stain-negative, rod-shaped, non-motile bacterial strain that produced flexirubin-type pigments, was isolated from Tibet Province, China, and characterized by using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the family Chitinophagaceae, phylum ‘Bacteroidetes’, and was related to members of the genus Niabella, with sequence similarities ranging from 94.1 to 96.4 %. Strain 15-4T contained MK-7 as the predominant menaquinone and its DNA G+C content was 46.9 mol%. The major fatty acids of strain 15-4T were iso-C15 : 0 (41.3 %), iso-C15 : 1 G (14.9 %), iso-C17 : 0 3-OH (13.2 %) and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH, 16.0 %). These chemotaxonomic data supported the affiliation of strain 15-4T to the genus Niabella. However, a number of physiological and biochemical features enabled the isolate to be differentiated phenotypically from recognized species of the genus Niabella. On the basis of the evidence presented, it is proposed that strain 15-4T represents a novel species, Niabella tibetensis sp. nov.; the type strain is 15-4T ( = CCTCC AB 209167T = NRRL B-59394T). On the basis of these data, an emended description of the genus Niabella is also proposed.

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