Taxonomy of Australian clinical isolates of the genus Photorhabdus and proposal of Photorhabdus asymbiotica subsp. asymbiotica subsp. nov. and P. asymbiotica subsp. australis subsp. nov.

The relationship of Photorhabdus isolates that were cultured from human clinical specimens in Australia to Photorhabdus asymbiotica isolates from human clinical specimens in the USA and to species of the genus Photorhabdus that are associated symbiotically with entomopathogenic nematodes was evaluated. A polyphasic approach that involved DNA–DNA hybridization, phylogenetic analyses of 16S rRNA and gyrB gene sequences and phenotypic characterization was adopted. These investigations showed that gyrB gene sequence data correlated well with DNA–DNA hybridization and phenotypic data, but that 16S rRNA gene sequence data were not suitable for defining species within the genus Photorhabdus. Australian clinical isolates proved to be related most closely to clinical isolates from the USA, but the two groups were distinct. A novel subspecies, Photorhabdus asymbiotica subsp. australis subsp. nov. (type strain, 9802892T=CIP 108025T=ACM 5210T), is proposed, with the concomitant creation of Photorhabdus asymbiotica subsp. asymbiotica subsp. nov. Analysis of gyrB sequences, coupled with previously published data on DNA–DNA hybridization and PCR-RFLP analysis of the 16S rRNA gene, indicated that there are more than the three subspecies of Photorhabdus luminescens that have been described and confirmed the validity of the previously proposed subdivision of Photorhabdus temperata. Although a non-luminescent, symbiotic isolate clustered consistently with P. asymbiotica in gyrB phylogenetic analyses, DNA–DNA hybridization indicated that this isolate does not belong to the species P. asymbiotica and that there is a clear distinction between symbiotic and clinical species of Photorhabdus.

Download Full-text

Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae)

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.018622-0 ◽

2011 ◽

Vol 61 (6) ◽

pp. 1360-1369 ◽

Cited By ~ 41

Author(s):

Arvind Kumar Gupta ◽

Mahesh Shantappa Dharne ◽

Ashraf Yusuf Rangrez ◽

Pankaj Verma ◽

Hemant V. Ghate ◽

...

Keyword(s):

16S Rrna ◽

Dna Hybridization ◽

Type Strain ◽

Gene Sequence ◽

Sequence Data ◽

Sequence Similarity ◽

23S Rrna ◽

Rrna Gene ◽

Respiratory Quinone ◽

Flesh Flies

Two Gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains FFA1T and FFA3T, and belonging to the class Gammaproteobacteria were isolated from the gastrointestinal tract of adult flesh flies (Diptera: Sarcophagidae). Phylogenetic analysis of 16S rRNA gene sequence data placed these two strains within the genus Ignatzschineria with similarities of 98.6 % (FFA1T) and 99.35 % (FFA3T) to Ignatzschineria larvae L1/68T. The level of gene sequence similarity between strains FFA1T and FFA3T was 99 %, 97.15 % and 78.1 % based on the 16S rRNA, 23S rRNA and gyrB gene sequences, respectively. Strains FFA1T and FFA3T shared 24 % DNA–DNA relatedness. DNA–DNA hybridization revealed a very low level of relatedness between the novel strains (22 % for strain FFA1T and 44 % for strain FFA3T) and I. larvae L1/68T genomic DNA. The respiratory quinone was Q-8 in both novel strains. The DNA G+C contents were 41.1 mol% and 40.1 mol% for strains FFA1T and FFA3T, respectively. The cell membrane of both strains consisted of phosphatidylglycerol, phosphatidylethanolamine, phospholipids and aminophospholipid. The major fatty acids for both strains were C16 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), CyC19 : 0ω8c and C14 : 0. The results of DNA–DNA hybridization between the two new strains and I. larvae L1/68T, in combination with phylogenetic, chemotaxonomic, biochemical and electron microscopic data, demonstrated that strains FFA1T and FFA3T represented two novel species of the genus Ignatzschineria for which the names Ignatzschineria indica sp. nov. (type strain FFA1T = DSM 22309T = KCTC 22643T = NCIM 5325T) and Ignatzschineria ureiclastica sp. nov. (type strain FFA3T = DSM 22310T = KCTC 22644T = NCIM 5326T) are proposed.

Download Full-text

Leptospira broomii sp. nov., isolated from humans with leptospirosis

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.63783-0 ◽

2006 ◽

Vol 56 (3) ◽

pp. 671-673 ◽

Cited By ~ 70

Author(s):

Paul N. Levett ◽

Roger E. Morey ◽

Renee L. Galloway ◽

Arnold G. Steigerwalt

Keyword(s):

16S Rrna ◽

Gel Electrophoresis ◽

Type Strain ◽

Gene Sequence ◽

Sequence Data ◽

Pulsed Field Gel Electrophoresis ◽

Rrna Gene ◽

Rrna Gene Sequence ◽

Dna Relatedness ◽

Gene Sequence Data

Isolates of Leptospira from two human cases of leptospirosis in Denmark and France were studied using DNA–DNA relatedness, G+C content, 16S rRNA gene sequence data and pulsed-field gel electrophoresis. These isolates differed from previously described species of Leptospira and are defined as Leptospira broomii sp. nov. The type strain is 5399T (=ATCC BAA-1107T=KIT 5399T).

Download Full-text

Molecular characterization and phylogenetic analyses of Tripartiella macrosoma Basson and Van As, 1987 and Tripartiella obtusa Ergens and Lom, 1970 based on 18S rRNA gene sequence data

Parasitology Research ◽

10.1007/s00436-021-07136-5 ◽

2021 ◽

Author(s):

Weiwei Qin ◽

Qian Zhou ◽

Shiyao Wang ◽

Fahui Tang ◽

Yuanjun Zhao

Keyword(s):

Molecular Characterization ◽

18S Rrna ◽

Gene Sequence ◽

Sequence Data ◽

Phylogenetic Analyses ◽

18S Rrna Gene ◽

Rrna Gene ◽

Rrna Gene Sequence ◽

Gene Sequence Data

Download Full-text

Phylogeny of Twenty Thermus Isolates Constructed from 16S rRNA Gene Sequence Data

International Journal of Systematic Bacteriology ◽

10.1099/00207713-43-4-754 ◽

1993 ◽

Vol 43 (4) ◽

pp. 754-760 ◽

Cited By ~ 48

Author(s):

D. J. SAUL ◽

A. G. RODRIGO ◽

R. A. REEVES ◽

L. C. WILLIAMS ◽

K. M. BORGES ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Gene Sequence ◽

Sequence Data ◽

16S Rrna Gene Sequence ◽

Rrna Gene ◽

Rrna Gene Sequence ◽

Gene Sequence Data

Download Full-text

Metagenomic, metatranscriptomics and 16S rRNA gene sequence data from diel sampling at Groves Creek Marsh, Skidaway Island, GA during July 2014

Biological and Chemical Oceanography Data Management Office ◽

10.1575/1912/bco-dmo.762443.1 ◽

2019 ◽

Author(s):

Alison Buchan

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Gene Sequence ◽

Sequence Data ◽

16S Rrna Gene Sequence ◽

Rrna Gene ◽

Rrna Gene Sequence ◽

Gene Sequence Data

Download Full-text

Range extension of Ichthyophis multicolor Wilkinson et al., 2014 to India and first molecular identification of Ichthyophis moustakius Kamei et al., 2009

Check List ◽

10.15560/17.4.1021 ◽

2021 ◽

Vol 17 (4) ◽

pp. 1021-1029

Author(s):

Hmar Tlawmte Lalremsanga ◽

Jayaditya Purkayastha ◽

Mathipi Vabeiryureilai ◽

Lal Muansanga ◽

Ht Decemson ◽

...

Keyword(s):

16S Rrna ◽

Gene Sequence ◽

Sequence Data ◽

Northeast India ◽

Range Extension ◽

Rrna Gene ◽

Rrna Gene Sequence ◽

Body Width ◽

New Material ◽

Gene Sequence Data

We report a substantial range extension of Ichthyophis multicolor Wilkinson, Presswell, Sherratt, Papadopoulou & Gower, 2014, with new material from Mizoram State, Northeast India. The species was previously known only from its type locality more than 800 km away in Ayeyarwady Region, Myanmar. The species was identified by both its morphology and 16s rRNA gene sequence data. One of the studied individuals represents the largest known specimen for the species (total length = 501 mm; mid-body width = 18.8 mm). Brief comparisons of I. multicolor with the sympatric as well as parapatric congeners in the region, and first barcode data for I. moustakius Kamei, Wilkinson, Gower & Biju, 2009 are also presented.

Download Full-text