Bacillus songklensis sp. nov., isolated from soil

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4189-4195 ◽  
Author(s):  
Hyeonji Kang ◽  
Veeraya Weerawongwiwat ◽  
Jong-Hwa Kim ◽  
Ampaitip Sukhoom ◽  
Wonyong Kim
Keyword(s):  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Distinct Lineage

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterial strain, designated CAU 1033T, was isolated from soil and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1033T formed a distinct lineage within the genus Bacillus and was most closely related to Bacillus drentensis KCTC 13025T (similarity 95.9 %). CAU 1033T contained MK-7 as the only isoprenoid quinone and iso-C15 : 0 and anteiso-C15 : 0 as the major fatty acids. The cell wall peptidoglycan of strain CAU 1033T contained meso-diaminopimelic acid and the major whole-cell sugars were arabinose, sucrose and ribose. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, four unidentified aminophospholipids, an unidentified aminolipid, two unidentified glycolipids and another unidentified polar lipid. The DNA G+C content was 41.4 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1033T was classified as a representative of a novel species in the genus Bacillus for which the name Bacillus songklensis sp. nov. is proposed. The type strain is CAU 1033T ( = KCTC 13881T = CCUG 61889T).

Haloferula chungangensis sp. nov., isolated from marine sediment

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 19-24 ◽  
Author(s):  
Hyeonji Kang ◽  
Jitsopin Traiwan ◽  
Veeraya Weerawongwiwat ◽  
Min Young Jung ◽  
Ji Hoon Jeong ◽  
...  
Keyword(s):  
Marine Sediment ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Distinct Lineage

A Gram-stain-negative, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated CAU 1074T, was isolated from marine sediment and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1074T grew optimally at 30 °C and pH 6.5. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1074T formed a distinct lineage within the genus Haloferula and was most closely related to Haloferula harenae KCTC 22198T (96.0 % similarity). Strain CAU 1074T contained MK-9 as the major isoprenoid quinone, and iso-C14 : 0, C16 : 1ω9c and C16 : 0 as the major fatty acids. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major whole-cell sugars were glucose, xylose, mannose and ribose. The polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminoglycolipid and two unidentified phospholipids. The DNA G+C content of the strain was 64.0 mol%. On the basis of phenotypic and chemotaxonomic data, and phylogenetic inference, strain CAU 1074T should be classified as a member of a novel species in the genus Haloferula , for which the name Haloferula chungangensis sp. nov. is proposed; the type strain is CAU 1074T ( = KCTC 23578T = CCUG 61920T). An emended description of the genus Haloferula is also provided.

Maribacter chungangensis sp. nov., isolated from a green seaweed, and emended descriptions of the genus Maribacter and Maribacter arcticus

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2553-2558 ◽  
Author(s):  
Veeraya Weerawongwiwat ◽  
Hyeonji Kang ◽  
Min Young Jung ◽  
Wonyong Kim
Keyword(s):  
Type Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Phenotypic Data ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Link Type ◽  
Green Seaweed ◽  
Distinct Lineage

A Gram-stain-negative, non-spore-forming, strictly aerobic, orange-pigmented bacterial strain, motile by gliding, designated CAU 1044T, was isolated from a green seaweed and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1044T formed a distinct lineage within the genus Maribacter and was most closely related to Maribacter antarcticus JCM 15445T and Maribacter arcticus KOPRI 20941T (96.3 and 95.7 % similarity, respectively). Strain CAU 1044T contained menaquinone 6 as the only isoprenoid quinone and iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH), iso-C17 : 0 3-OH and iso-C15 : 1 G as the major fatty acids. The cell wall peptidoglycan of strain CAU 1044T contained meso-diaminopimelic acid and the major whole-cell sugars were glucose and ribose. The polar lipids were composed of phosphatidylethanolamine, one unidentified phospholipid, six unidentified aminolipids and four unidentified lipids. The DNA G+C content was 40.2 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1044T should be classified as a representative of a novel species in the genus Maribacter for which the name Maribacter chungangensis sp. nov. is proposed. The type strain is CAU 1044T ( = KCTC 23735T  = CCUG 61948T). Emended descriptions of the genus Maribacter and the species M. arcticus KCTC 22053T are also proposed.

scholarly journals Streptomyces arboris sp. nov., isolated from Populus euphratica wetland soil

2020 ◽  
Vol 70 (11) ◽  
pp. 5613-5619 ◽  
Author(s):  
Panpan Liu ◽  
Zhanfeng Xia ◽  
Lili Zhang
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Populus Euphratica ◽  
Diaminopimelic Acid ◽  
Wetland Soil ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type

A novel actinobacterial strain (TRM 68085T) was isolated from soil of Populus euphratica wetland. A polyphasic approach was used to study the taxonomy of TRM 68085T and the results showed a range of phylogenetic and chemotaxonomic properties consistent with those of the genus Streptomyces . Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 68085T showed the highest similarity value to Streptomyces capitiformicae 1H-SSA4T (98.6 %), and phylogenetically clustered with Streptomyces kanasensis ZX01T(97.5 %) and Streptomyces ipomoeae NBRC 13050T (97.4 %). The genomic DNA G+C content of strain TRM 68085T based on the genome sequence was 71.4 mol%. The levels of DNA–DNA relatedness between the genome of the isolate and its nearest phylogenetic neighbours, S. capitiformicae 1H-SSA4T, S. kanasensis ZX01T and S. ipomoeae NBRC 13050T, were 19.2±0.4, 21.8±0.5 and 19.3±0.6 %, respectively. Chemotaxonomic data revealed that strain TRM 68085T possessed MK-9(H6) and MK-9(H8) as the predominant menaquinones. ll-Diaminopimelic acid and a small amount of meso-diaminopimelic acid were the diagnostic diamino acids. Ribose, xylose, glucose and galactose were the whole-cell sugars. The major cellular fatty acids were C16 : 0 (25.4 %) and iso-C16 : 0 (18.3 %). On the basis of these genotypic and phenotypic data, it is concluded that strain TRM 68085T represents a novel species of the genus Streptomyces , for which the name Streptomyces arboris sp. nov. is proposed. The type strain is TRM 68085T (=CCTCC AA2019031T=LMG 31492T).

scholarly journals Bacillus mesonae sp. nov., isolated from the root of Mesona chinensis

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3346-3352 ◽  
Author(s):  
Bo Liu ◽  
Guo-Hong Liu ◽  
Gui-Hing Hu ◽  
Mei-Chun Chen
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph

A Gram-stain-positive, short rod-shaped and motile, mildly halotolerant, endospore-forming bacterium, FJAT-13985T, was isolated from the internal tissues of Mesona chinensis root. Strain FJAT-13985T grew at 20–45 °C (optimum 30 °C) and pH 5.7–9.0 (optimum pH 7.0) and in the presence of 0–2 % (w/v) NaCl [optimum 1 % (w/v)]. The strain was catalase-positive and oxidase-negative. The cell wall of strain FJAT-13985T contained meso-diaminopimelic acid and the predominant isoprenoid quinone was MK-7 (97.4 %). The major fatty acids of the strain were anteiso-C15 : 0 (23.3 %) and iso-C15 : 0 (40.8 %). The DNA G+C content was 41.64 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FJAT-13985T is a member of the genus Bacillus and is most closely related to Bacillus drentensis DSM 15600T (98.4 %), Bacillus vireti DSM 15602T (98.2 %) and Bacillus novalis DSM 15603T (98.3 %). DNA–DNA hybridization indicated that relatedness between strain FJAT-13985T and its closest relative, B. drentensis DSM 15600T, was 36.63 %. The phenotypic, chemotaxonomic and genotypic properties clearly indicate that strain FJAT-13985T represents a novel species of the genus Bacillus , for which the name Bacillus mesonae sp. nov. is proposed. The type strain is FJAT-13985T ( = DSM 25968T = CGMCC1.12238T).

Limosilactobacillus urinaemulieris sp. nov. and Limosilactobacillus portuensis sp. nov. isolated from urine of healthy women

2019 ◽  
Vol 71 (3) ◽  
Author(s):  
Magdalena Ksiezarek ◽  
Teresa Gonçalves Ribeiro ◽  
Joana Rocha ◽  
Filipa Grosso ◽  
Svetlana Ugarcina Perovic ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Healthy Women ◽  
Genome Distance

Two Gram-stain-positive strains, c9Ua_26_MT and c11Ua_112_MT, were isolated from voided urine samples from two healthy women. Comparative 16S rRNA gene sequences demonstrated that these novel strains were members of the genus Limosilactobacillus . Phylogenetic analysis based on pheS gene sequences and core genomes showed that each strain formed a separated branch and are closest to Limosilactobacillus vaginalis DSM 5837T. The average nucleotide identity (ANI) and Genome-to-Genome Distance Calculator (GGDC) values between c9Ua_26_MT and the closest relative DSM 5837T were 90.7 and 42.9 %, respectively. The ANI and GGDC values between c11Ua_112_MT and the closest relative DSM 5837T were 91.2 and 45.0 %, and those among the strains were 92.9% and 51,0 %, respectively. The major fatty acids were C12 : 0 (40.2 %), C16 : 0 (26.7 %) and C18 : 1 ω9c (17.7 %) for strain c9Ua_26_MT, and C18 : 1 ω9c (38.0 %), C16 : 0 (33.3 %) and C12 : 0 (17.6 %) for strain c11Ua_112_MT. The genomic DNA G+C content of strains c9Ua_26_MT and c11Ua_112_MT was 39.9 and 39.7 mol%, respectively. On the basis of the data presented here, strains c9Ua_26_MT and c11Ua_112_MT represent two novel species of the genus Limosilactobacillus , for which the names Limosilactobacillus urinaemulieris sp. nov. (c9Ua_26_MT=CECT 30144T=LMG 31899T) and Limosilactobacillus portuensis sp. nov. (c11Ua_112_MT=CECT 30145T=LMG 31898T) are proposed.

Arthrobacter cheniae and Arthrobacter frigidicola sp. nov., isolated from a glacier

2021 ◽  
Vol 71 (12) ◽  
Author(s):  
Lei-Lei Yang ◽  
Hong-Can Liu ◽  
Qing Liu ◽  
Yu-Hua Xin
Keyword(s):  
Fatty Acids ◽  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Average Nucleotide Identity ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Unidentified Glycolipid

Two Gram-stain-positive, aerobic, rod-shaped, pink and light pink colony-forming bacteria, designated as Hz2T and MDT2-14T, respectively, were isolated from glacier cryoconite samples. Comparisons based on 16S rRNA gene sequences showed that strains Hz2T and MDT2-14T take Arthrobacter bussei KR32T and Arthrobacter zhaoguopingii J391T as their closest neighbours, respectively. The average nucleotide identity values between the two novel strains and their closest relatives were 83.56 and 93.06 %, respectively. The two strains contain MK-9(H2) as their predominant menaquinone. The polar lipids of both strains were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The major fatty acids of strain Hz2T were anteiso-C15 : 0, summed feature 3 (comprising C16 : 1  ω7c and/or C16 : 1  ω6c) and iso-C15 : 0, while the major fatty acids of strain MDT2-14T were anteiso-C15 : 0 and anteiso-C17 : 0. Based on these data, we propose two novel species, Arthrobacter cheniae sp. nov. (Hz2T = CGMCC 1.9262T=NBRC 113086T) and Arthrobacter frigidicola sp. nov. (MDT2-14T=CGMCC 1.9882T=NBRC 113089T).

Dactylosporangium siamense sp. nov., isolated from soil

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4033-4038 ◽  
Author(s):  
Chitti Thawai ◽  
Chanwit Suriyachadkun
Keyword(s):  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Mycolic Acids ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Tropical Forest Soil ◽  
Actinomycete Strain

A novel actinomycete strain, designed MW4-36T, was isolated from tropical forest soil in Nakhon Sawan Province, Thailand. Morphological and chemotaxonomic characteristics of this strain clearly demonstrated that it belongs to the genus Dactylosporangium . The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan contained glutamic acid, glycine, alanine and meso-diaminopimelic acid including 3-hydroxy-meso-diaminopimelic acid; arabinose, glucose, rhamnose, ribose and xylose were found as whole-cell sugars. The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol; no phosphatidylcholine was found. The predominant menaquinones were MK-9(H8) and MK-9(H6). Mycolic acids were not detected. The predominant cellular fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 72.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences also indicated that the strain should be classified in the genus Dactylosporangium and showed that the closest relative was Dactylosporangium maewongense JCM 15933T (99.4 % similarity). These taxonomic data revealed that strain MW4-36T could be readily distinguished from its phylogenetically closest relative. On the basis of these phenotypic and genotypic data, strain MW4-36T is considered to represent a novel species, for which the name Dactylosporangium siamense sp. nov. is proposed. The type strain is MW4-36T ( = BCC 34901T = NBRC 106093T).

Gemmobacter megaterium sp. nov., isolated from coastal planktonic seaweeds

2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 66-71 ◽  
Author(s):  
Jin-Jin Liu ◽  
Xin-Qi Zhang ◽  
Fang-Tao Chi ◽  
Jie Pan ◽  
Cong Sun ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Sea Salts ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type

A Gram-stain-negative, non-motile and aerobic bacterium, designated CF17T, was isolated from coastal planktonic seaweeds, East China Sea. The isolate grew at 18–37 °C (optimum 25–28 °C), pH 6.5–9.0 (optimum 7.0–8.0) and with 0–5 % NaCl (optimum 1–2 %, w/v) and 0.5–10 % sea salts (optimum 2–3 %, w/v). Growth of strain CF17T could be stimulated prominently by supplementing the growth medium with the autoclaved supernatant of a culture of strain CF5, which was isolated from the same sample along with strain CF17T. The cell morphology of strain CF17T was a bean-shaped rod consisting of a swollen end and a long prostheca. The phylogenetic analysis of 16S rRNA gene sequences indicated that strain CF17T clustered with Gemmobacter nectariphilus DSM 15620T within the genus Gemmobacter . The DNA G+C content of strain CF17T was 61.4 mol%. The respiratory quinone was ubiquinone Q-10. The major fatty acids included C18 : 1ω7c and C18 : 0. The polar lipids of strain CF17T consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two uncharacterized phospholipids, one uncharacterized aminolipid, three uncharacterized glycolipids and one uncharacterized lipid. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain CF17T ( = CGMCC 1.11024T = JCM 18498T) is considered to represent a novel species of the genus Gemmobacter , for which the name Gemmobacter megaterium sp. nov. is proposed.

scholarly journals Flavisolibacter rigui sp. nov., isolated from freshwater of an artificial reservoir and emended description of the genus Flavisolibacter

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4038-4042 ◽  
Author(s):  
Keun Sik Baik ◽  
Mi Sun Kim ◽  
Ji Hee Lee ◽  
Sang Suk Lee ◽  
Wan-Taek Im ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A non-motile and rod-shaped bacterium, designated strain 02SUJ3T, was isolated from freshwater collected from the Juam Reservoir (Republic of Korea). Cells were Gram-stain-negative, aerobic, oxidase-negative and catalase-positive. The major fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH. The strain contained MK-7 as the major isoprenoid quinone. The main polar lipid was phosphatidylethanolamine. The DNA G+C content was 46.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 02SUJ3T forms an independent lineage within the genus Flavisolibacter with low sequence similarity to Flavisolibacter ginsengiterrae Gsoil 492T and Flavisolibacter ginsengisoli Gsoil 643T (95.7 %). Phenotypic characteristics distinguished strain 02SUJ3T from members of the genus Flavisolibacter . On the basis of the evidence presented in this study, strain 02SUJ3T is considered to represent a novel species, for which the name Flavisolibacter rigui sp. nov. is proposed. The type strain is 02SUJ3T ( = JCM 17515T = KCTC 23328T). An emended description of the genus Flavisolibacter is also provided.

Flavobacterium jumunjinense sp. nov., isolated from a lagoon, and emended descriptions of Flavobacterium cheniae , Flavobacterium dongtanense and Flavobacterium gelidilacus

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 3937-3943 ◽  
Author(s):  
Yochan Joung ◽  
Haneul Kim ◽  
Kiseong Joh
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Polar Lipids ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Distinct Lineage ◽  
Motile By Gliding

A Gram-staining-negative, yellow-pigmented bacterial strain, motile by gliding, designated HME7102T, was isolated from lagoon water in Korea. The phylogenetic tree based on 16S rRNA gene sequences showed that strain HME7102T formed a distinct lineage within the genus Flavobacterium . Strain HME7102T was closely related to Flavobacterium cheniae NJ-26T (94.5 %), Flavobacterium dongtanense LW30T (94.4 %) and Flavobacterium gelidilacus LMG 21477T (94.2 %). The major fatty acids of strain HME7102T were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH. The major polyamine was homospermidine and the major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and two unidentified polar lipids. The DNA G+C content of strain HME7102T was 36.5 mol%. On the basis of the evidence presented in this study, strain HME7102T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium jumunjinense sp. nov. is proposed. The type strain is HME7102T ( = KCTC 23618T = CECT 7955T). Emended descriptions of F. cheniae , F. dongtanense and F. gelidilacus are also proposed.

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