scholarly journals Sinosporangium siamense sp. nov., isolated from soil and emended description of the genus Sinosporangium

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2828-2833 ◽  
Author(s):  
Chanwit Suriyachadkun ◽  
Wipaporn Ngaemthao ◽  
Suwanee Chunhametha ◽  
Chitti Thawai ◽  
Jean-Jacques Sanglier ◽  
...  
Keyword(s):  
Deciduous Forest ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Dry Deciduous Forest ◽  
Genotypic Characteristics

An actinomycetes strain A-T 1946T that developed spherical sporangia containing non-motile spores on aerial mycelia was isolated from dry deciduous forest soil in Thailand. 16S rRNA gene sequence analysis indicated that strain A-T 1946T belongs to the genus Sinosporangium , being closely related to Sinosporangium album 6014T (98.8 % sequence similarity). The DNA–DNA relatedness values were 43.7–50.9 %, which were significantly below 70 % and differentiated strain A-T 1946T from the closest species. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinone was MK-9(H2). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannoside, N-acetylglucosamine-containing phospholipids, two unknown phosphoglycolipids and two unknown phospholipids. The predominant cellular fatty acids were unsaturated C16 : 1 and C17 : 1, and saturated C16 : 0 and 10-methyl-C17 : 0. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the isolate is proposed to represent a novel species of genus Sinosporangium to be named Sinosporangium siamense sp. nov. The type strain is A-T 1946T ( = BCC 29081T = NBRC 109515T). An emended description of the genus Sinosporangium is also provided.

scholarly journals Bacteroides sartorii is an earlier heterotypic synonym of Bacteroides chinchillae and has priority

2012 ◽  
Vol 62 (Pt_6) ◽  
pp. 1241-1244 ◽  
Author(s):  
Mitsuo Sakamoto ◽  
Moriya Ohkuma
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Single Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Emended Description

Strains of the recently proposed species Bacteroides chinchillae share more than 99.4 % 16S rRNA gene sequence similarity with the type strain of Bacteroides sartorii although these two species do not appear to be similar from their published descriptions. The aim of this study was to perform phenotypic and genetic analyses of both species to clarify their taxonomic position. B. chinchillae JCM 16497T exhibited high hsp60 gene sequence similarity with B. sartorii JCM 17136T (100 %) as well as B. chinchillae JCM 16498 (100 %). The hsp60 gene sequence analysis and levels of DNA–DNA relatedness observed demonstrated B. sartorii JCM 17136T, B. chinchillae JCM 16497T, and B. chinchillae JCM 16498 are members of a single species. Based on these data, we propose Bacteroides chinchillae as a later heterotypic synonym of Bacteroides sartorii . An emended description of B. sartorii is provided.

scholarly journals Description of Domibacillus indicus sp. nov., isolated from ocean sediments and emended description of the genus Domibacillus

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3010-3015 ◽  
Author(s):  
Avinash Sharma ◽  
Sunil Kumar Dhar ◽  
Om Prakash ◽  
Venkata Ramana Vemuluri ◽  
Vishal Thite ◽  
...  
Keyword(s):  
Cell Wall ◽  
Type Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Marine Sediment Sample

A novel Gram-stain-positive, spore-forming, aerobic, non-motile, rod-shaped bacterium designated strain SD111T that forms red-pigmented colonies was isolated from a marine sediment sample (collected from 5 m depth) from Lakshadweep, India. Strain SD111T grew well on seawater agar at pH 6–10 (optimum pH 7.5±0.2). It showed maximum (97.6 %) 16S rRNA gene sequence similarity and formed a monophyletic clade with Domibacillus robiginosus WS 4628T ( = DSM 25058T). The genomic DNA G+C content was 37.4 mol% and the strain showed 37.7 % DNA–DNA relatedness to D. robiginosus DSM 25058T. The major fatty acids were anteiso-C15 : 0, C16 : 0, iso-C15 : 0 and iso-C16 : 0 and MK-6 was the predominant quinone. The polar lipid profile of strain SD111T consisted of unidentified phospholipids (PL1 and PL2), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG). The cell wall contained meso-diaminopimelic acid and the peptidoglycan was of A1γ type. Glucose and ribose were detected as major cell-wall sugars. Results from polyphasic studies indicated that SD111T represents a novel species of the genus Domibacillus for which the name Domibacillus indicus sp. nov. is proposed. The type strain is SD111T ( = MCC 2255T = DSM 28032T).

scholarly journals Kutzneria buriramensis sp. nov., isolated from soil, and emended description of the genus Kutzneria

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 47-52 ◽  
Author(s):  
Chanwit Suriyachadkun ◽  
Wipaporn Ngaemthao ◽  
Suwanee Chunhametha ◽  
Tomohiko Tamura ◽  
Jean-Jacques Sanglier
Keyword(s):  
Deciduous Forest ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A Gram-staining-positive, filamentous bacterium, which developed large globose sporangia at the ends of long sporangiophores on aerial mycelium, was isolated from dry soil collected in a deciduous forest in Thailand. The cell-wall peptidoglycan of the novel bacterium, which was designated strain A-T 1846T, contained meso-diaminopimelic acid and the whole-cell sugars comprised rhamnose, ribose, mannose, glucose and galactose. The predominant menaquinone was MK-9(H4). Mycolic acids were not detected. The identified phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol. The predominant cellular fatty acids were iso-C16 : 0, iso-C16 : 0 2-OH and C16 : 0 10-methyl. The G+C content of the genomic DNA was 72 mol%. The phenotypic and chemotaxonomic characteristics of the novel strain resembled those of established members of the genus Kutzneria . Phylogenetic analyses based on 16S rRNA gene sequences also indicated that this strain belonged to the genus Kutzneria but as a representative of a putative novel species. In hybridization experiments, the DNA–DNA relatedness values recorded between the novel strain and established members of the genus Kutzneria all fell well below 70 %. Based on the phenotypic, chemotaxonomic and genotypic evidence and the results of the DNA–DNA hybridizations, strain A-T 1846T represents a novel species in the genus Kutzneria , for which the name Kutzneria buriramensis sp. nov. is proposed. The type strain is A-T 1846T ( = BCC 29373T  = NBRC 107931T). An emended description of the genus Kutzneria is provided.

Halopelagius longus sp. nov., a member of the family Halobacteriaceae isolated from a salt mine, and emended description of the genus Halopelagius

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3585-3590 ◽  
Author(s):  
Xin Zhang ◽  
Wei-Yan Zhang ◽  
Ai-Hua Shen ◽  
Ying-Yi Huo ◽  
Xu-Fen Zhu ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Salt Mine ◽  
Content Type ◽  
Link Type ◽  
Nacl Concentration ◽  
Emended Description ◽  
Extremely Halophilic Archaeon ◽  
Genotypic Characteristics

A thermotolerant, extremely halophilic archaeon, BC12-B1T, was isolated from a salt mine in Baicheng county, Xinjiang province, China. Colonies were off-white–grey. The cells stained Gram-negative, were motile and irregularly long-rod-shaped (variation in both width and length) with abundant gas vesicles. The strain was able to grow at 20–55 °C (optimum, 48 °C), at pH 6.0–8.0 (optimum, 7.0–7.3), with 1.8–6.0 M NaCl (optimum, 3.0–3.5 M) and with 0.02–2.2 M Mg2+ (optimum, 0.1–0.2 M). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain BC12-B1T was most closely related to Halopelagius inordinatus RO5-2T (98.5 %) with less than 95 % sequence similarity to other described species. The genomic DNA G+C content of strain BC12-B1T was 64.0 mol%. The DNA–DNA hybridization value between strain BC12-B1T and Hpl. inordinatus RO5-2T was 43.6 %. The major polar lipids of strain BC12-B1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, four glycolipids and an unknown lipid. Based on phenotypic, chemotaxonomic and genotypic characteristics, strain BC12-B1T represents a novel species of the genus Halopelagius , for which the name Halopelagius longus sp. nov. is proposed. The type strain is BC12-B1T ( = CGMCC 1.12397T = JCM 18758T). An emended description of the genus Halopelagius is also provided.

scholarly journals Sinosporangium fuscu m sp. nov., isolated from soil

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1764-1769 ◽  
Author(s):  
Chanwit Suriyachadkun ◽  
Wipaporn Ngaemthao ◽  
Suwanee Chunhametha ◽  
Chitti Thawai ◽  
Jean-Jacques Sanglier
Keyword(s):  
Type Species ◽  
Diaminopimelic Acid ◽  
Evergreen Forest ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Unknown Phospholipid ◽  
Genotypic Characteristics ◽  
Phosphatidylinositol Mannosides

A novel actinomycete, A-T 8343T was isolated from a moist evergreen forest soil sample collected in the Trat Province, Thailand. Based on 16S rRNA gene sequence analysis, strain A-T 8343T belonged to the genus Sinosporangium and was closely related to Sinosporangium siamense A-T 1946T (98.81 %) and Sinosporangium album 6014T (98.54 %). The DNA–DNA relatedness values were 21.8–27 % with S. siamense A-T 1946T and 31.1–31.9 % with S. album 6014T, which were significantly below 70 %. The result differentiated A-T 8343T from the closest species. The organism developed spherical sporangia containing non-motile spores on aerial mycelia. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinones were MK-9(H2) and MK-9(H4). The diagnostic phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, diphosphatidylglycerol, lyso-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannosides, aminophosphoglycolipid and one unknown phospholipid. The major cellular fatty acids were saturated C16 : 0, iso C16 : 0, unsaturated C16 : 1 and C18 : 1. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the new isolate is proposed as a representative novel species of the genus Sinosporangium to be named Sinosporangium fuscum sp. nov. The type strain is A-T 8343T ( = BCC 52770T = NBRC 109516T).

scholarly journals Nocardioides daecheongensis sp. nov., isolated from soil

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4109-4114 ◽  
Author(s):  
Jun-Muk Lim ◽  
Soo-Jin Kim ◽  
Moriyuki Hamada ◽  
Jae-Hyung Ahn ◽  
Hang-Yeon Weon ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Closely Related Species ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Gene Sequence Analysis ◽  
Type Strains

Strain KIS2-16T was isolated from a soil sample collected from Daecheong Island of Incheon region, South Korea. KIS2-16T was Gram-staining-positive, aerobic, non-spore-forming, non-motile, catalase-positive, oxidase-negative and mesophilic. On the basis of 16S rRNA gene sequence analysis, strain KIS2-16T represented a member of the genus Nocardioides , being most closely related to the type strains of species of the genus Nocardioides , Nocardioides maradonensis RP-B30T (97.8 % sequence similarity) and Nocardioides ultimimeridianus RP-B26T (97.0 %). The fatty acid profile of KIS2-16T was dominated by C18 : 1ω9c, C17 : 1ω8c, C16 : 0, C18 : 0 10-methyl (TBSA), C16 : 0 2-OH and C17 : 0 2-OH. The major isoprenoid quinone was MK-8(H4), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The peptidoglycan structure was A3γ-type with ll-diaminopimelic acid. The genomic DNA G+C content of KIS2-16T was 64.9 mol%. Strain KIS2-16T showed DNA–DNA hybridization values of less than 70 % with the closely related species of the genus Nocardioides . Based on phenotypic, genotypic and phylogenetic data, the isolate represents a novel species of the genus Nocardioides , for which the name Nocardioides daecheongensis sp. nov. (type strain KIS2-16T = DSM 27136T = KACC 17297T = NBRC 109597T) is proposed.

scholarly journals Planobispora takensis sp. nov., isolated from soil

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1180-1185 ◽  
Author(s):  
Wipaporn Ngaemthao ◽  
Chanwit Suriyachadkun ◽  
Suwanee Chunhametha ◽  
Nantawan Niemhom ◽  
Chitti Thawai ◽  
...  
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
Evergreen Forest ◽  
Rrna Gene ◽  
The Novel ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Genotypic Characteristics

A novel Gram-stain–positive, filamentous bacterial strain (A-T 7458T) was isolated from soil collected from hill evergreen forest in Thailand. The strain developed cylindrical sporangia containing a longitudinal pair of motile spores, on short ramifications of aerial mycelia. From a taxonomic study using a polyphasic approach, strain A-T 7458T had typical characteristics of members of the genus Planobispora . 16S rRNA gene sequence analysis indicated that the isolate was closely related to Planobispora siamensis A-T 4600T (98.5 %), Planobispora rosea JCM 3166T (97.6 %) and Planobispora longispora NBRC 13918T (97.6 %). The DNA–DNA relatedness values, which differentiated the novel strain from the closest species, were significantly below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinone was MK-9(H2). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17 : 1 and C18 : 1, and saturated C16 : 0 and C17 : 0. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the novel isolate is proposed to represent a novel species of the genus Planobispora , to be named Planobispora takensis sp. nov. The type strain is A-T 7458T ( = BCC 48396T = NBRC 109077T).

scholarly journals Streptomyces gossypiisoli sp. nov., isolated from cotton soil in Xinjiang, PR China

2020 ◽  
Author(s):  
Qiao-Yan Zhang ◽  
Song Qin ◽  
Xiao-Xia Luo ◽  
Zhan-Feng Xia
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Streptomyces Chromofuscus ◽  
Pr China ◽  
Diamino Acid

A novel actinobacterium, designated TRM 44567T, was isolated from cotton soil in Xinjiang Uygur Autonomous Region, northwest PR China. Growth occurred at 16–45 °C, pH 5.0–9.0, and 0–7 % (w/v) NaCl, with optimum growth at 37 °C, pH 7.0–8.0 and 1 % (w/v) NaCl, respectively. Comparative 16S rRNA gene sequence analysis indicated that strain TRM 44567T was phylogenetically most closely related to Streptomyces chromofuscus NBRC 12851T (98.48 % sequence similarity); however, the average nucleotide identity between strain TRM 44567T and S. chromofuscus NBRC 12851T was only 83.77 %. Strain TRM 44567T possessed ll-diaminopimelic acid as the diagnostic cell-wall diamino acid. The predominant menaquinones were MK-9(H10), MK-9(H6) and MK-9(H4). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C15 : 0, anteiso-C17 : 0, iso-C14 : 0 and iso-C15 : 0. The genomic DNA G+C content was 70.8 mol%. Strain TRM 44567T represents a novel species of the genus Streptomyces , for which the name Streptomyces gossypiisoli sp. nov. is proposed. The type strain is TRM 44567T (=KCTC 39957 T=CCTCC AA 2017011T).

Amycolatopsis dendrobii sp. nov., an endophytic actinomycete isolated from Dendrobium heterocarpum Lindl.

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
Nisachon Tedsree ◽  
Somboon Tanasupawat ◽  
Boonchoo Sritularak ◽  
Nattakorn Kuncharoen ◽  
Kittisak Likhitwitayawuid
Keyword(s):  
Cell Wall ◽  
Type Species ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Taxonomic Approach ◽  
Genotypic Characteristics

Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach. The strains grew at 20–37 °C, at pH 5–10 and with 5 % (w/v) NaCl. They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone. Arabinose and galactose were the major sugars in the cell wall. The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0. The detected polar lipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylglycerol. Strains DR6-1T, DR6-2 and DR6-4 shared 99.9–100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%). The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%. The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively. Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp. nov. is proposed. The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).

scholarly journals Angustibacter aerolatus sp. nov., isolated from air

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 610-615 ◽  
Author(s):  
Soo-Jin Kim ◽  
Yun-Hee Jang ◽  
Moriyuki Hamada ◽  
Tomohiko Tamura ◽  
Jae-Hyung Ahn ◽  
...  
Keyword(s):  
Cell Wall ◽  
Sequence Similarity ◽  
Cellular Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Actinomycete Strain ◽  
Unknown Lipid ◽  
Genotypic Characteristics

A novel actinomycete strain, 7402J-48T, was isolated from an air sample collected from Jeju island, Republic of Korea. Cells were Gram-positive, aerobic, flagellated, short rods. Strain 7402J-48T showed highest 16S rRNA gene sequence similarity (98.6 %) with Angustibacter luteus TT07R-79T, and had relatively low sequence similarities (below 95.1 %) with other members of the family Kineosporiaceae . The cell wall of strain 7402J-48T contained alanine, glutamic acid and 2,6-diaminopimelic acid, suggesting A1γ-type peptidoglycan. The menaquinones were MK-9(H4) and MK-8(H4). The acyl type of the cell-wall muramic acid was acetyl. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unknown lipid were present. The cellular fatty acid profile comprised a large amount of anteiso-C15 : 0, moderate amounts of C16 : 0, summed feature 3 (including C16 : 1ω7c and/or C16 : 1ω6c), C17 : 0, iso-C15 : 0, C16 : 0 N alcohol and C17 : 1ω8c, and small amounts of other fatty acids. The DNA G+C content of strain 7402J-48T was 73 mol%. Based on its phenotypic and genotypic characteristics, strain 7402J-48T ( = KACC 15527T = NBRC 108730T) is proposed as the type strain of a novel species, Angustibacter aerolatus sp. nov. An emended description of the genus Angustibacter is provided.

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