scholarly journals Celeribacter marinus sp. nov., isolated from coastal seawater

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1323-1327 ◽  
Author(s):  
Kiwoon Baek ◽  
Ahyoung Choi ◽  
Ilnam Kang ◽  
Jang-Cheon Cho
Keyword(s):  
Type Species ◽  
Optimal Growth ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Sequence Comparisons ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Ubiquinone 10

A Gram-staining-negative, non-motile, non-pigmented and rod-shaped bacterial strain, designated IMCC12053T, was isolated from coastal surface seawater of the Yellow Sea, Korea. Optimal growth of strain IMCC12053T was observed at 30 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. 16S rRNA gene sequence comparisons showed that strain IMCC12053T was most closely related to Celeribacter baekdonensis L-6T (97.5 % similarity) and Celeribacter neptunius H 14T (96.0 %). Strain IMCC12053T formed a robust phylogenetic clade with members of the genus Celeribacter . The DNA–DNA relatedness value between IMCC12053T and C. baekdonensis was far lower than 70 % (35.7–42.5 %), which indicated that strain IMCC12053T is a novel genomic species of the genus Celeribacter . The major respiratory isoprenoid quinone was ubiquinone-10 (Q-10) and major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and aminolipids. The DNA G+C content was 61.0 mol%. On the basis of genotypic and phenotypic data collected in this study, it is proposed that strain IMCC12053T represents a novel species of the genus Celeribacter , for which the name Celeribacter marinus sp. nov. is proposed. The type strain is IMCC12053T ( = KACC 17482T = NBRC 109702T).

scholarly journals Phenylobacterium kunshanense sp. nov., isolated from the sludge of a pesticide manufacturing factory

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 325-330 ◽  
Author(s):  
Cuiwei Chu ◽  
Cansheng Yuan ◽  
Xin Liu ◽  
Li Yao ◽  
Jianchun Zhu ◽  
...  
Keyword(s):  
Type Species ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Comparisons ◽  
Content Type ◽  
Link Type ◽  
Ubiquinone 10 ◽  
Sequence Similarities

A novel aerobic, Gram-stain-negative, motile bacterium, designated strain BUT-10T, was isolated from the sludge of a pesticide manufacturing factory in Kunshan, China. Cells were rod-shaped (0.4–0.45×0.9–1.4 µm) and colonies were white, circular with entire edges and had a smooth surface. The strain grew at 25–37 °C, at pH 6.0–8.0 and with 0–0.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BUT-10T was a member of the genus Phenylobacterium , and showed highest sequence similarities to Phenylobacterium muchangponense A8T (97.49 %), Phenylobacterium immobile DSM 1986T (97.14 %) and Phenylobacterium lituiforme FaiI3T (96.34 %). Major fatty acids (>5 %) were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The major isoprenoid quinone was ubiquinone-10. The DNA G+C content was 71.85 mol%. Strain BUT-10T showed low DNA–DNA relatedness with P. muchangponense A8T (15.7±2.9 %) and P. immobile DSM 1986T (12.8±1.1 %). On the basis of the phenotypic, phylogenetic and genotypic data, strain BUT-10T is considered to represent a novel species of the genus Phenylobacterium , for which the name Phenylobacterium kunshanense sp. nov. is proposed. The type strain is BUT-10T ( = CCTCC AB 2013085T = KCTC 42014T).

Sphingobacterium hotanense sp. nov., isolated from soil of a Populus euphratica forest, and emended descriptions of Sphingobacterium daejeonense and Sphingobacterium shayense

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 815-820 ◽  
Author(s):  
Ting Xiao ◽  
Xiang He ◽  
Gang Cheng ◽  
Haiju Kuang ◽  
Xiangru Ma ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Populus Euphratica ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Gram Staining

A novel Gram-staining-negative bacterial strain, designated XH4T, was isolated from soil of a Populus euphratica forest in the Hotan River valley, Xinjiang Uyghur autonomous region, PR China. The cells were strictly aerobic, non-motile, short rods. The isolate grew optimally at 37 °C and at pH 7.0–8.0. Based on 16S rRNA gene sequence analysis, strain XH4T belonged to the genus Sphingobacterium and was closely related to Sphingobacterium mizutaii ATCC 33299T (96.1 % sequence similarity). The DNA G+C content was 41.2 mol%. The major polar lipid of strain XH4T was phosphatidylethanolamine, and several unidentified polar lipids were also present. Strain XH4T showed the typical chemotaxonomic features of the genus Sphingobacterium , with the presence of ceramide phosphorylethanolamine 2 (CerPE-2) as the major ceramide. The major cellular fatty acids of strain XH4T were iso-C15 : 0 (34.0 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 20.7 %) and iso-C17 : 0 3-OH (14.7 %). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic data and phylogenetic inference, strain XH4T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium hotanense sp. nov. is proposed. The type strain is XH4T ( = NRRL B-59204T  = CCTCC AB 209007T). Emended descriptions of Sphingobacterium daejeonense and Sphingobacterium shayense are also given.

scholarly journals Description of Lysinibacillus sinduriensis sp. nov., and transfer of Bacillus massiliensis and Bacillus odysseyi to the genus Lysinibacillus as Lysinibacillus massiliensis comb. nov. and Lysinibacillus odysseyi comb. nov. with emended description of the genus Lysinibacillus

2012 ◽  
Vol 62 (Pt_10) ◽  
pp. 2347-2355 ◽  
Author(s):  
Min Young Jung ◽  
Joong-Su Kim ◽  
Woon Kee Paek ◽  
Igor Styrak ◽  
In-Soon Park ◽  
...  
Keyword(s):  
Cell Wall ◽  
Related Species ◽  
Type Species ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Group 2 ◽  
Phenotypic Tests

A Gram-positive, rod-shaped, endospore-forming bacterium, designated strain BLB-1T, was isolated from samples of tidal flat sediment from the Yellow Sea. 16S rRNA gene sequence analysis demonstrated that the isolate belonged to the Bacillus rRNA group 2 and was closely related to Bacillus massiliensis CIP 108446T (97.4 %), Bacillus odysseyi ATCC PTA-4993T (96.7 %), Lysinibacillus fusiformis DSM 2898T (96.2 %) and Lysinibacillus boronitolerans DSM 17140T (95.9 %). Sequence similarities with related species in other genera, including Caryophanon , Sporosarcina and Solibacillus , were <96.1 %. Chemotaxonomic data supported the affiliation of strain BLB-1T with the genus Lysinibacillus . The major menaquinone was MK-7, the cell-wall sugars were glucose and xylose, the cell-wall peptidoglycan type was A4α (l-Lys–d-Asp), the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown phospholipids, and the major fatty acids were anteiso-C15 : 0 (35.6 %), iso-C15 : 0 (25.6 %) and anteiso-C17 : 0 (16.5 %). The most closely related species, Bacillus massiliensis and Bacillus odysseyi , were also assigned to this genus based on phylogenetic analysis and phenotypic data. The results of DNA–DNA hybridizations and phenotypic tests supported the differentiation of all three taxa from species of the genus Lysinibacillus with validly published names. Thus, strain BLB-1T ( = KCTC 13296T  = JCM 15800T) represents a novel species, for which the name Lysinibacillus sinduriensis sp. nov. is proposed. It is also proposed that Bacillus massiliensis CIP 108446T ( = 4400831T = CCUG49529T  = KCTC 13178T) and Bacillus odysseyi NBRC 100172T ( = 34hs-1T  = ATCC PTA-4993T  = NRRL B-30641T  = DSM 18869T  = CIP 108263T  = KCTC 3961T) be transferred to the genus Lysinibacillus as Lysinibacillus massiliensis comb. nov. and Lysinibacillus odysseyi comb. nov., respectively.

scholarly journals Paracoccus sediminilitoris sp. nov., isolated from a tidal flat sediment

2019 ◽  
Vol 69 (4) ◽  
pp. 1035-1040 ◽  
Author(s):  
Yuli Wei ◽  
Junwei Cao ◽  
Huimin Yao ◽  
Haiyan Mao ◽  
Kelei Zhu ◽  
...  
Keyword(s):  
Tidal Flat ◽  
Dna Hybridization ◽  
Type Species ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Content Type ◽  
Link Type ◽  
Tidal Flat Sediment

A novel marine Gram-stain-negative, non-spore-forming, motile, aerobic, coccoid or ovoid bacterium, designated as strain DSL-16T, was isolated from a tidal flat sediment on the East China Sea and characterized phylogenetically and phenotypically. Optimal growth of the strain occurred at 35 °C (range 4–40 °C), at pH 6 (range 5–11) and with 4 % (w/v) NaCl (range 1–14 %). The nearest phylogenetic neighbour was Paracoccus seriniphilus DSM 14827T (98.2 % 16S rRNA gene sequence similarity). The digital DNA–DNA hybridization value between strain DSL-16T and P. seriniphilus DSM 14827T was 19.5±2.2 %. The average nucleotide identity value between strain DSL-16T and P. seriniphilus DSM 14827T was 83.6 %. The sole respiratory ubiquinone was Q-10. The major polar lipids were phosphatidylmonomethylethanolamine (PME), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), diphosphatidyglycerol (DPG) and glycolipid (GL). The predominant cellular fatty acids of strain DSL-16T were C18 : 1ω7c, C18 : 0 and 11-methyl C18 : 1ω7c. The G+C content of the genomic DNA was 64.5 mol%. The combined genotypic and phenotypic data indicated that strain DSL-16T represents a novel species of the genus Paracoccus , for which the name Paracoccus sediminilitoris sp. nov. is proposed. The type strain is DSL-16T (=KCTC 62644T=MCCC 1K03534T).

scholarly journals Flavobacterium daemonensis sp. nov., isolated from Daemo Mountain soil

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 983-989 ◽  
Author(s):  
Hien T. T. Ngo ◽  
MooChang Kook ◽  
Tae-Hoo Yi
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Sequence Comparisons ◽  
Content Type ◽  
Phylogenetic Characterization ◽  
Link Type ◽  
Gram Staining ◽  
Bright Yellow

A Gram-staining-negative, catalase- and oxidase-positive, obligately aerobic, motile by gliding, bright yellow and rod-shaped bacterial strain was isolated from soil of Daemo Mountain (Daemosan) in Seoul, Republic of Korea. Its taxonomic position was investigated by using a polyphasic study. On the basis of 16S rRNA gene sequence comparisons, strain THG–DJ7T was found to be most closely related to Flavobacterium denitrificans ED5T (97.9 % sequence similarity), Flavobacterium ginsenosidimutans THG 01T (97.6 %), Flavobacterium kyungheensis THG-107T (97.5 %), Flavobacterium anhuiense D3T (97.1 %) and Flavobacterium ginsengisoli DCY54T (97.0 %). The DNA–DNA relatedness between strain THG–DJ7T and its phylogenetically closest neighbours was below 40.0 %. The G+C content of the genomic DNA was determined to be 32.6 mol%. The only isoprenoid quinone detected in strain THG–DJ7T was menaquinone-6 (MK-6). The major component in the polyamine pattern was sym-homospermidine. The major polar lipids were found to be phosphatidylethanolamine, phosphatidyldimethylethanolamine, an unidentified aminophosphoglycolipid, phosphatidylserine and an unidentified lipid. The major fatty acids were identified as iso-C15 : 0, iso-C15 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). These data support the affiliation of strain THG–DJ7T to the genus Flavobacterium . We report the phenotypic, genotypic and phylogenetic characterization of strain THG–DJ7T. Based on the findings presented, a novel species of the genus Flavobacterium , Flavobacterium daemonensis sp. nov. is proposed, with THG–DJ7T ( = KACC 17651T = JCM 19455T) as the type strain.

scholarly journals Novosphingobium aquiterrae sp. nov., isolated from ground water

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3282-3287 ◽  
Author(s):  
Jae-Chan Lee ◽  
Song-Gun Kim ◽  
Kyung-Sook Whang
Keyword(s):  
Ground Water ◽  
Type Species ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Polyphasic Analysis ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Gene Sequence Analysis

A Gram-staining-negative, strictly aerobic, non-motile, non-spore-forming, yellow and rod-shaped bacterium, designated E-II-3T, was isolated from ground water at Daejeon in Korea. Strain E-II-3T grew between 4 and 45 °C (optimal growth at 28 °C), between pH 6.0 and 9.0 (optimal growth at pH 7.5) and at salinities of 0–2.0 % (w/v) NaCl, growing optimally with 0.5 % (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain E-II-3T was shown to belong to the genus Novosphingobium and showed closest phylogenetic similarity to ‘Novosphingobium ginsenosidimutans’ FW-6 (97.7 %), Novosphingobium aromaticivorans F199T (96.9 %) and Novosphingobium subterraneum B0478T (96.5 %). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The predominant ubiquinone and polyamine components were Q-10 and spermidine, respectively. The major fatty acids were C18 : 1ω7c (34.0 %), C16 : 1ω7c and/or iso-C15 : 0 2-OH (23.8 %) and C17 : 1ω6c (19.3 %). The DNA G+C content of this novel isolate was 62.7 mol%. DNA–DNA relatedness between strain E-II-3T and ‘N. ginsenosidimutans’ KACC 16615, N. aromaticivorans KCTC 2888T and N. capsulatum KCTC 22844T was 38, 33 and 29 %, respectively. On the basis of polyphasic analysis from this study, strain E-II-3T represents a novel species of the genus Novosphingobium for which the name Novosphingobium aquiterrae sp. nov. is proposed. The type strain is E-II-3T ( = KACC 17599T = NBRC 109812T = NCAIM B 02537T).

Vibrio ziniensis sp. nov., isolated from mangrove sediments

2021 ◽  
Vol 71 (4) ◽  
Author(s):  
Jie Wu ◽  
Wu Qu ◽  
Qiliang Lai ◽  
Shengxiang Pei ◽  
Tianyou Zhang ◽  
...  
Keyword(s):  
Type Species ◽  
Novel Species ◽  
Whole Genome Sequence ◽  
Rrna Gene ◽  
Mangrove Sediments ◽  
Sequence Comparisons ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Pr China

A novel Gram-staining-negative, catalase- and oxidase-positive, facultatively anaerobic and rod-shaped motile bacterial strain, designated as ZWAL4003T, was isolated from mangrove sediments of the Zini Mangrove Forest, Zhangzhou City, PR China. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that ZWAL4003T was grouped into a separated branch with Vibrio plantisponsor MSSRF60T (97.38% nucleotide sequence identity) and Vibrio diazotrophicus NBRC 103148T (97.27%). The major cellular fatty acids were C14 : 0 (12.6%), C16 : 0 (17.6%), and summed feature 3 (C16 : 1ω6c /C16 : 1 ω7c, 45.6%). Its genome had a length of 4650556 bp with 42.8% DNA G+C content, and contained genes involved in the biosynthesis of bacteriocin, β-lactone, resorcinol, N-acyl amino acid, and arylpolyene. The in silico DNA–DNA hybridization and average nucleotide identity values for whole-genome sequence comparisons between ZWAL4003T and V. plantisponsor LMG 24470T were clearly below the thresholds used for the delineation of a novel species. The morphological and chemotaxonomic characteristics and the genotypic data of ZWAL4003T indicated that it represented a novel species of the genus Vibrio . Its proposed name is Vibrio ziniensis sp. nov., and the type strain is ZWAL4003T (=KCTC 72971T=MCCC 1A17474T).

scholarly journals Mesonia aquimarina sp. nov., a marine bacterium isolated from coastal seawater

2015 ◽  
Vol 65 (Pt_1) ◽  
pp. 135-140 ◽  
Author(s):  
Ahyoung Choi ◽  
Kiwoon Baek ◽  
Hansol Lee ◽  
Jang-Cheon Cho
Keyword(s):  
Type Species ◽  
Marine Bacterium ◽  
Optimal Growth ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Close Relationship

A Gram-staining-negative, aerobic, yellow, non-flagellated, non-gliding, rod-shaped bacterium, designated strain IMCC1021T, was isolated off the coast of the East Sea. Optimal growth of strain IMCC1021T was observed at 25 °C, pH 7.0–7.5 and in the presence of 3.0–3.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain belonged to the genus Mesonia , showing a close relationship with Mesonia mobilis KMM 6059T (97.4 % similarity) followed by Mesonia phycicola MDSW-25T (96.1 %). DNA–DNA relatedness between strain IMCC1021T and M. mobilis KMM 6059T was 17.5 % (reciprocal 7.4 %), which indicated that the new isolate represents a novel genomic species of the genus Mesonia . The major fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH, and the G+C content of the genomic DNA was 41.4 mol%. Strain IMCC1021T contained menaquinone-6 (MK-6) as the respiratory quinone, and polar lipids comprising phosphatidylethanolamine, two unknown aminolipids and three unknown polar lipids. On the basis of the phylogenetic distinctions and differential phenotypic characteristics, it is suggested that strain IMCC1021T ( = KCTC 32320T = NBRC 109485T) should be assigned to the genus Mesonia as the type strain of a novel species, for which the name Mesonia aquimarina sp. nov. is proposed.

Kordia antarctica sp. nov., isolated from Antarctic seawater

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3617-3622 ◽  
Author(s):  
Kiwoon Baek ◽  
Ahyoung Choi ◽  
Ilnam Kang ◽  
Kiyoung Lee ◽  
Jang-Cheon Cho
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
The Antarctic

A Gram-staining-negative, chemoheterotrophic, yellow-pigmented, non-motile, flexirubin-negative, facultatively anaerobic bacterium, designated strain IMCC3317T, was isolated from a coastal seawater sample from the Antarctic Penninsula. Optimal growth of strain IMCC3317T was observed at 20 °C, pH 8.0 and in the presence of 2–3 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMCC3317T belonged to the genus Kordia and was closely related to Kordia algicida OT-1T (96.7 % sequence similarity) and Kordia periserrulae IMCC1412T (96.1 % sequence similarity). The major fatty acids were 10-methyl C16 : 0 and/or iso-C16 : 1ω9c, iso-C17 : 0 3-OH, iso-C15 : 0 and anteiso-C15 : 0. The G+C content of the genomic DNA was 35.1 mol%. The strain contained menaquinone-6 (MK-6) as the respiratory quinone. The polar lipids detected in the strain were phosphatidylethanolamine and unknown aminophospholipids, aminolipids and polar lipids. On the basis of phylogenetic distinction and differential phenotypic characteristics, it is suggested that strain IMCC3317T ( = KCTC 32292T = NBRC 109401T) be assigned to the genus Kordia as the type strain of a novel species, for which the name Kordia antarctica sp. nov. is proposed.

Muricauda zhangzhouensis sp. nov., isolated from mangrove sediment

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2320-2325 ◽  
Author(s):  
Caiyun Yang ◽  
Yi Li ◽  
Qian Guo ◽  
Qiliang Lai ◽  
Jun Wei ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Optimal Growth ◽  
Bacterial Consortium ◽  
Mangrove Sediment ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining

A Gram-staining-negative, rod-shaped and non-motile bacterial strain, designated 12C25T, was isolated from the crude-oil-degrading bacterial consortium enriched from mangrove sediment collected in Fujian Province, China. Optimal growth was observed at 25–28 °C, at pH 7.0 and in the presence of 2 % (w/v) NaCl + 2 % (w/v) KCl. Comparative 16S rRNA gene sequence analysis demonstrated that strain 12C25T shared the highest sequence similarity with members of the genus Muricauda (97.7–93.9 %), exhibiting 97.7 % sequence similarity and 33.7±4 % DNA–DNA relatedness to Muricauda aquimarina SW-63T. The DNA G+C content of strain 12C25T was 39.9 mol%. The dominant fatty acids were iso-C15 : 1 G, iso-C17 : 0 3-OH, iso-C15 : 0, C18 : 0 and iso-C15 : 0 3-OH, and menaquinone with six isoprene units (MK-6) was the only respiratory quinone. On the basis of phenotypic data and phylogenetic inference, the novel strain belongs to the genus Muricauda , but can readily be distinguished from known species of this genus and thus represents a novel species of the genus Muricauda. The name Muricauda zhangzhouensis sp. nov. is proposed and the type strain is 12C25T ( = CGMCC 1.11028T = MCCC 1F01096T = DSM 25030T).

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