scholarly journals Neptunomonas acidivorans sp. nov., isolated from sediment, and emended description of the genus Neptunomonas

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3650-3654 ◽  
Author(s):  
Sung-Hyun Yang ◽  
Hyun-Seok Seo ◽  
Jung-Hyun Lee ◽  
Sang-Jin Kim ◽  
Kae Kyoung Kwon
Keyword(s):  
The Yellow Sea ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Optimum Ph ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene

A Gram-reaction-negative, aerobic, rod-shaped (1.2–1.6 µm×0.6–0.8 µm), flagellated and motile marine bacterium, designated MEBiC06243T, was isolated from a sediment collected at Daebu Island in the Yellow Sea (37° 20′ N 126° 41′ E), Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC06243T showed high similarity with Neptunomonas naphthovorans NAG-2N-126T (96.3 %). Growth was observed at 10–39 °C (optimum 29 °C), at pH 6.0–9.0 (optimum pH 7) and with 0–7 % (optimum 2.5 %) NaCl. The predominant cellular fatty acids were C10 : 0 3-OH (6.1 %), C12 : 0 (5.8 %), C16 : 0 (30.5 %), C18 : 1ω7c (21.6 %) and summed feature 3 (comprising C15 : 0 2-OH and/or C16 : 1ω7c; 30.7 %). The DNA G+C content was 41.4 mol%. The major respiratory quinone was Q-8. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified lipids, one unidentified aminophospholipid and three unidentified aminolipids were detected as major polar lipids. On the basis of this polyphasic taxonomic data, strain MEBiC06243T should be classified as a novel species of the genus Neptunomonas proposed as Neptunomonas acidivorans sp. nov. The type strain is MEBiC06243T ( = KCCM 42975T = JCM 18291T). An emended description of the genus Neptunomonas is also given.

scholarly journals Kiloniella spongiae sp. nov., isolated from a marine sponge and emended description of the genus Kiloniella Wiese et al. 2009 and Kiloniella laminariae

2015 ◽  
Vol 65 (Pt_1) ◽  
pp. 230-234 ◽  
Author(s):  
Sung-Hyun Yang ◽  
Hyun-Seok Seo ◽  
Jung-Hyun Lee ◽  
Sang-Jin Kim ◽  
Kae Kyoung Kwon
Keyword(s):  
Type Species ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Optimum Ph ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene

A Gram-reaction-negative, aerobic, rod-shaped and non-motile marine bacterium, designated MEBiC09566T was isolated from a sponge collected at Uljin County in the coastal area of the East Sea (36° 55′ N, 129° 25′ E), Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC09566T showed the highest similarity with the Kiloniella laminariae LD81T (96.7 %). Growth was observed at 11–31 °C (optimum 25 °C), at pH 6.0–8.5 (optimum pH 7.0) and with 0–6 % (optimum 2.5 %) NaCl. The predominant cellular fatty acids were summed feature 8 (comprised of C18 : 1ω7c/C18 : 1ω6c) and summed feature 3 (comprised of C16 : 1ω7c and/or C16 : 1ω6c). The DNA G+C content is 44.6 mol%. The major respiratory quinone is Q-9. Phosphatidylethanolamine, phosphatidylglycerol, an unidentified lipid, two unidentified aminophospholipids and one unidentified aminolipid were detected as major polar lipids. On the basis of this polyphasic taxonomic data, it is concluded that strain MEBiC09566T should be classified as representing a novel species in the genus Kiloniella and the name proposed is Kiloniella spongiae sp. nov. The type strain is MEBiC09566T ( = KCCM 43040T = JCM 19930T). Emended descriptions of the genus Kiloniella Wiese et al. 2009 and Kiloniella laminariae are also given.

scholarly journals Marivirga atlantica sp. nov., isolated from seawater and emended description of the genus Marivirga

2015 ◽  
Vol 65 (Pt_5) ◽  
pp. 1515-1519 ◽  
Author(s):  
Chao-Yi Lin ◽  
Xi-Ying Zhang ◽  
Ang Liu ◽  
Chang Liu ◽  
Xiao-Yan Song ◽  
...  
Keyword(s):  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Polyphasic Characterization ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

A novel Gram-stain-negative, aerobic, orange-pigmented, non-flagellated, gliding, rod-shaped bacterium, designated strain SM1354T was isolated from surface seawater of the Atlantic Ocean. The strain hydrolysed gelatin and DNA but did not reduce nitrate. It grew at 4–40 °C and with 0.5–11 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene sequences revealed that strain SM1354T belonged to the genus Marivirga with 96.0–96.2 % sequence similarities to known species of the genus Marivirga . The major fatty acids of strain SM1354T were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 03-OH and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 02-OH). Polar lipids of strain SM1354T included phosphatidylethanolamine, three unidentified lipids and one unidentified aminolipid and aminophospholipid. The major respiratory quinone of strain SM1354T was menaquinone 7 (MK-7). The genomic DNA G+C content of strain SM1354T was 33.9±0.4 mol%. On the basis of the results of the polyphasic characterization in this study, it is proposed that strain SM1354T represents a novel species of the genus Marivirga , namely Marivirga atlantica sp. nov. The type strain of Marivirga atlantica is SM1354T ( = CCTCC AB 2014242T = JCM 30305T). An emended description of the genus Marivirga is also proposed.

Brumimicrobium mesophilum sp. nov., isolated from a tidal flat sediment, and emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 1105-1110 ◽  
Author(s):  
Sung-Hyun Yang ◽  
Hyun-Seok Seo ◽  
Hyun-Myung Oh ◽  
Sang-Jin Kim ◽  
Jung-Hyun Lee ◽  
...  
Keyword(s):  
Tidal Flat ◽  
Type Species ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Major Respiratory Quinone

A Gram-stain-negative, aerobic, rod-shaped, non-motile and orange-coloured marine bacterium, YH207T, was isolated from a tidal flat at Yeongheung-do on the coast of the Yellow Sea, Korea. 16S rRNA gene sequence analysis revealed that strain YH207T was affiliated with the family Cryomorphaceae and showed highest similarity to Brumimicrobium glaciale IC156T (95.4 %). Growth was observed at 11–36 °C, at pH 6.5–10.0 and with 0.4–7.0 % NaCl. The predominant cellular fatty acids when grown at 20 °C were iso-C15 : 0 (44.2 %), iso-C15 : 1 G (34.3 %), iso-C17 : 0 3-OH (8.7 %) and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c; 2.3 %). The major respiratory quinone was MK-6. Phosphatidylethanolamine, phosphatidylglycerol, three unidentified lipids, three unidentified aminophospholipids, one unidentified phospholipid, four unidentified aminolipids and three unidentified glycolipids were identified as major polar lipids. The DNA G+C content was 34.3 mol%. On the basis of the data from our polyphasic taxonomic study, strain YH207T should be classified in a novel species in the genus Brumimicrobium , for which the name Brumimicrobium mesophilum sp. nov. is proposed. The type strain is YH207T ( = KCCM 42331T  = JCM 14063T). Emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale Bowman et al. 2003 are also given.

scholarly journals Cohnella arctica sp. nov., isolated from Arctic tundra soil

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 817-821 ◽  
Author(s):  
Fan Jiang ◽  
Jun Dai ◽  
Yang Wang ◽  
Xiuqing Xue ◽  
Mengbo Xu ◽  
...  
Keyword(s):  
Rrna Gene ◽  
Tundra Soil ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Optimum Ph ◽  
Major Respiratory Quinone ◽  
Type Strains ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

A psychrotolerant Gram-reaction-negative, rod-shaped and orange-pigmented bacterium, designated strain M9-62T, which was motile by means of peritrichous flagella, was isolated from tundra soil sampled near Ny-Ålesund, Svalbard Islands, Norway (78° N). Growth occurred at 4–30 °C (optimum, 25 °C) and pH 5.0–8.0 (optimum, pH 6.0–7.0). Analysis of the 16S rRNA gene sequence of strain M9-62T placed it in the genus Cohnella ; sequence similarities of the isolate with type strains of members of related genera ranged from 92.0 to 96.3 %. Strain M9-62T contained anteiso-C15 : 0 (51.1 %), iso-C16 : 0 (7.5 %) and C16 : 0 (6.1 %) as the major cellular fatty acids and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol as the main polar lipids. The major respiratory quinone was MK-7 and the DNA G+C content was 50.3 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain M9-62T is considered to represent a novel species of the genus Cohnella , for which the name Cohnella arctica sp. nov. is proposed; the type strain is M9-62T ( = CCTCC AB 2010228T = NRRL B-59459T).

Description of Kordiimonas aquimaris sp. nov., isolated from seawater, and emended descriptions of the genus Kordiimonas Kwon et al. 2005 emend. Xu et al. 2011 and of its existing species

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 298-302 ◽  
Author(s):  
Seong-Hyun Yang ◽  
Mi-Ree Kim ◽  
Hyun-Seok Seo ◽  
Sung Hyuk Lee ◽  
Jung-Hyun Lee ◽  
...  
Keyword(s):  
Type Species ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Optimum Ph ◽  
Phyletic Line ◽  
Major Respiratory Quinone

A Gram-negative, aerobic, rod-shaped (0.6–0.9×0.7–1.2 µm), motile marine bacterium, designated MEBiC06554T, was isolated from seawater collected from the East Sea, Korea (also known as the Sea of Japan). 16S rRNA gene sequence analysis revealed that strain MEBiC06554T was affiliated to the order Kordiimonadales and showed high similarity to Kordiimonas gwangyangensis GW14-5T (95.4 %), but formed a distinct phyletic line. Growth was observed at 10.5–35.0 °C (optimum 20 °C), at pH 5.0–8.0 (optimum pH 7.0) and with 0–13 % (w/v) NaCl (optimum 3.0–3.5 %). The predominant cellular fatty acids were iso-C15 : 0 (14.9 %), iso-C17 : 1ω9c (44.6 %), iso-C17 : 0 (6.3 %) and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c; 13.9 %). The DNA G+C content was 50.3 mol%. The major respiratory quinone was Q-10. Phosphatidylethanolamine, two unidentified glycolipids, six unidentified aminolipids, three unidentified lipids and one unidentified aminophospholipid were detected as major polar lipids. On the basis of the data from our polyphasic taxonomic study, strain MEBiC06554T should be classified within a novel species of the genus Kordiimonas , as Kordiimonas aquimaris sp. nov. The type strain is MEBiC06554T ( = KCCM 42940T  = JCM 16665T). Emended descriptions of the genus Kordiimonas and of its species Kordiimonas gwangyangensis and Kordiimonas lacus are also proposed.

Orbus sasakiae sp. nov., a bacterium isolated from the gut of the butterfly Sasakia charonda, and emended description of the genus Orbus

2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1766-1770 ◽  
Author(s):  
Joon Yong Kim ◽  
Jina Lee ◽  
Na-Ri Shin ◽  
Ji-Hyun Yun ◽  
Tae Woong Whon ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Bacterium Strain ◽  
Sasakia Charonda

A novel Gram-stain-negative, facultatively anaerobic, non-motile and coccus-shaped bacterium, strain C7T, was isolated from the gut of the butterfly Sasakia charonda. Strain C7T grew optimally at 20–25 °C, at pH 7–8 and with 1 % (w/v) NaCl. The strain was negative for oxidase activity but positive for catalase activity. The 16S rRNA gene sequences of strain C7T and Orbus hercynius CN3T shared 96.8 % similarity. The major fatty acids identified were C14 : 0, C16 : 0, C18 : 1ω7c and summed feature 2 (comprising C14 : 0 3-OH/iso-C16 : 1). The major respiratory quinone was ubiquinone-8 (Q-8). The polar lipids of strain C7T were phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid and two unidentified aminophospholipids. The G+C content of the genomic DNA extracted from strain C7T was 32.1 mol%. Taken together, the phenotypic, genotypic and phylogenetic analyses indicate that strain C7T represents a novel species of the genus Orbus , for which the name Orbus sasakiae sp. nov. is proposed. The type strain is C7T ( = KACC 16544T = JCM 18050T). An emended description of the genus Orbus is provided.

scholarly journals Halioglobus maricola sp. nov., isolated from coastal seawater

2020 ◽  
Vol 70 (3) ◽  
pp. 1868-1875 ◽  
Author(s):  
Shan-Hui Li ◽  
Jaeho Song ◽  
Yeonjung Lim ◽  
Yochan Joung ◽  
Ilnam Kang ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
A Genome ◽  
The 16S Rrna Gene

A Gram-stain-negative, rod-shaped, aerobic, non-flagellated, chemoheterotrophic bacterium, designated IMCC14385T, was isolated from surface seawater of the East Sea, Republic of Korea. The 16S rRNA gene sequence analysis indicated that IMCC14385T represented a member of the genus Halioglobus sharing 94.6–97.8 % similarities with species of the genus. Whole-genome sequencing of IMCC14385T revealed a genome size of 4.3 Mbp and DNA G+C content of 56.7 mol%. The genome of IMCC14385T shared an average nucleotide identity of 76.6 % and digital DNA–DNA hybridization value of 21.6 % with the genome of Halioglobus japonicus KCTC 23429T. The genome encoded the complete poly-β-hydroxybutyrate biosynthesis pathway. The strain contained summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C17 : 1 ω8c as the predominant cellular fatty acids as well as ubiquinone-8 (Q-8) as the respiratory quinone. The polar lipids detected in the strain were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, five unidentified phospholipids, an unidentified aminolipid, an unidentified aminophospholipid and four unidentified lipids. On the basis of taxonomic data obtained in this study, it is suggested that IMCC14385T represents a novel species of the genus Halioglobus , for which the name Halioglobus maricola sp. nov. is proposed. The type strain is IMCC14385T (=KCTC 72520T=NBRC 114072T).

scholarly journals Sphingobacterium paludis sp. nov., isolated from wetland soil

2014 ◽  
Vol 64 (Pt_10) ◽  
pp. 3453-3458 ◽  
Author(s):  
Hao Feng ◽  
Yanhua Zeng ◽  
Yili Huang
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Wetland Soil ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis ◽  
Optimum Ph ◽  
Xixi Wetland

A novel Gram-stain-negative bacteria, designated S37T, was isolated from soil of the Xixi wetland, Zhejiang province, China. Cells of strain S37T were aerobic, non-motile rods. Growth occurred at 10–37 °C (optimum, 25 °C), pH 5.0–9.7 (optimum, pH 7.5) and with 0–6 % (w/v) NaCl (optimum, 0.5 %). Based on 16S rRNA gene sequence analysis, strain S37T was found to be a member of the genus Sphingobacterium and shared highest similarity with Sphingobacterium composti 4M24T (95.78 %). The major fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), iso-C15 : 0 and iso-C17 : 0 3-OH, and the DNA G+C content was 43.8 mol%. The predominant respiratory quinone was MK-7. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain S37T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium paludis sp. nov. (type strain S37T = CGMCC 1.12801T = NBRC 110386T) is proposed.

Ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of chick pea (Cicer arietinum L.)

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4484-4488 ◽  
Author(s):  
R. Kathiravan ◽  
S. Jegan ◽  
V. Ganga ◽  
V. R. Prabavathy ◽  
L. Tushar ◽  
...  
Keyword(s):  
16S Rrna ◽  
Rhizosphere Soil ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Ensifer Adhaerens ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene

The taxonomic position of strain MSSRFBL1T, isolated from chickpea rhizosphere soil from Kannivadi, India, was determined. Strain MSSRFBL1T formed bluish black colonies, stained Gram-negative and was motile, aerobic, capable of fixing dinitrogen, oxidase-negative and catalase-positive. Q-10 was the major respiratory quinone. Major fatty acids of strain MSSRFBL1T were C18 : 1ω7c and C19 : 0cycloω8c. Minor amounts of C18 : 0, C12 : 0, C14 : 0 3-OH, C18 : 0 3-OH, C16 : 0, C16 : 1ω6c/C16 : 1ω7c, C17 : 0 3-OH and C20 : 1ω7c were also present. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine and two unidentified glycolipids. Bacteriohopane derivatives (BHD1 and 2), diplopterol, diploptene, bishomohopanediol, adenosylhopane and 2β-methyl bacteriohopanetetrol were the major hopanoids of strain MSSRFBL1T. The genomic DNA G+C content was 71 mol%. EzTaxon-e-based blast analysis of the 16S rRNA gene indicated the highest similarity of strain MSSRFBL1T to Ensifer adhaerens LMG 20216T (97.3 %) and other members of the genus Ensifer (<96.9 %) in the family Rhizobiaceae of the class Alphaproteobacteria . However, phylogenetic analysis based on 16S rRNA, recA, thrC and dnaK gene sequences showed distinct out-grouping from the recognized genera of the family Rhizobiaceae . Based on phenotypic, genotypic and chemotaxonomic characters, strain MSSRFBL1T represents a novel species in a new genus in the family Rhizobiaceae for which the name Ciceribacter lividus gen. nov., sp. nov. is proposed. The type strain of Ciceribacter lividus is MSSRFBL1T ( = DSM 25528T = KCTC 32403T).

Silanimonas mangrovi sp. nov., a member of the family Xanthomonadaceae isolated from mangrove sediment, and emended description of the genus Silanimonas

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 274-279 ◽  
Author(s):  
T. N. R. Srinivas ◽  
T. B. Kailash ◽  
Pinnaka Anil Kumar
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Mangrove Sediment ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Emended Description ◽  
Major Respiratory Quinone

A novel Gram-negative, rod-shaped, motile bacterium, designated strain AK13T, was isolated from a sediment sample collected from mangrove of Namkhana, Sunderbans, West Bengal, India. Strain AK13T was positive for oxidase, DNase and lipase activities and negative for catalase, gelatinase, ornithine decarboxylase, lysine decarboxylase, nitrate reductase, aesculinase and urease activities. The fatty acids were dominated by iso-C11 : 0, iso-C11 : 0 3-OH, iso-C15 : 0, iso-C16 : 0, iso-C17 : 1ω9c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). Strain AK13T contained Q-8 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, two unidentified aminolipids, one unidentified glycolipid and one unidentified lipid as the polar lipids. The DNA G+C content of strain AK13T was 55.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the type strain of Silanimonas lenta , of the family Xanthomonadaceae (phylum Proteobacteria ), was the closest neighbour of strain AK13T, with 95.2 % sequence similarity. Other members of the family showed sequence similarities <94.4 %. Based on the phenotypic characteristics and phylogenetic inference, strain AK13T is proposed as a member of a novel species of the genus Silanimonas , Silanimonas mangrovi sp. nov.; the type strain is AK13T ( = MTCC 11082T  = DSM 24914T). An emended description of the genus Silanimonas is also provided.

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