scholarly journals A new genus of the family Micromonosporaceae, Polymorphospora gen. nov., with description of Polymorphospora rubra sp. nov.

2006 β—½  
Vol 56 (8) β—½  
pp. 1959-1964 β—½  
Author(s):  
Tomohiko Tamura β—½  
Kazunori Hatano β—½  
Ken-ichiro Suzuki
Keyword(s):  
New Genus β—½  
Diaminopimelic Acid β—½  
Rrna Gene β—½  
The Novel β—½  
Phenotypic Properties β—½  
The Family β—½  
Isoprenoid Quinones β—½  
Mangrove Roots β—½  
Family Based β—½  
Distinct Taxon

Two actinomycete strains were isolated from soil surrounding mangrove roots. The isolates formed short spore chains with spores showing diverse shapes. The isolates contained glutamic acid, glycine, alanine and meso-diaminopimelic acid in the cell wall, 3-O-methylmannose, mannose, galactose and glucose as the whole-cell sugars and MK-10(H6), MK-10(H4), MK-9(H6) and MK-9(H4) as the predominant isoprenoid quinones. The isolates formed a distinct taxon in the phylogenetic tree of the Micromonosporaceae based on analysis of 16S rRNA gene sequences and showed chemical and phenotypic properties that were different from members of all of the other genera of this family. Based on these observations, it is proposed that the novel isolates belong to a new genus, Polymorphospora gen. nov. The type species of the genus is proposed as Polymorphospora rubra sp. nov., with strain TT 97-42T (=NBRC 101157T=DSM 44947T) as the type strain.

scholarly journals Amphibacillus sediminis sp. nov., an endospore-forming bacterium isolated from lake sediment in Japan

2007 β—½  
Vol 57 (11) β—½  
pp. 2489-2492 β—½  
Author(s):  
Sun-Young An β—½  
Shu Ishikawa β—½  
Hiroaki Kasai β—½  
Keiichi Goto β—½  
Akira Yokota
Keyword(s):  
Lake Sediment β—½  
Type Strain β—½  
Diaminopimelic Acid β—½  
Phylogenetic Position β—½  
Rrna Gene β—½  
The Novel β—½  
Phenotypic Properties β—½  
Diamino Acid β—½  
Isoprenoid Quinones β—½  
Type Strains

A facultatively anaerobic, moderately alkaliphilic, Gram-positive, spore-forming and rod-shaped bacterial strain, Shu-P-Ggiii25-2T, was isolated from lake sediment in Shizuoka, Japan, and was characterized taxonomically using a polyphasic approach. Analysis of the 16S rRNA gene sequence indicated that the novel isolate clustered with the type strain of Amphibacillus xylanus and it exhibited sequence similarities of 94.9–95.6β€Š% to the type strains of species of the genus Amphibacillus. Isoprenoid quinones and oxidase and catalase activities were not detected for strain Shu-P-Ggiii25-2T. The DNA G+C content was 42.3β€…mol%, the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and the major cellular fatty acids were iso-C14β€Š:β€Š0, iso-C15β€Š:β€Š0, anteiso-C15β€Š:β€Š0, C16β€Š:β€Š0 and iso-C16β€Š:β€Š0. On the basis of phylogenetic position and phenotypic properties, strain Shu-P-Ggiii25-2T represents a novel species of the genus Amphibacillus, for which the name Amphibacillus sediminis sp. nov. is proposed. The type strain is Shu-P-Ggiii25-2T (=MBIC08269T=IAM 15428T=KCTC 13120T). An emended description of the genus Amphibacillus is also given.

scholarly journals Shimazuella kribbensis gen. nov., sp. nov., a mesophilic representative of the family Thermoactinomycetaceae

2007 β—½  
Vol 57 (11) β—½  
pp. 2660-2664 β—½  
Author(s):  
Dong-Jin Park β—½  
Syed G. Dastager β—½  
Jae-Chan Lee β—½  
Soo-Hwan Yeo β—½  
Jung-Hoon Yoon β—½  
...  
Keyword(s):  
16S Rrna β—½  
16S Rrna Gene β—½  
New Genus β—½  
Aerial Mycelium β—½  
Diaminopimelic Acid β—½  
Rrna Gene β—½  
The Novel β—½  
16S Rrna Gene Sequences β—½  
The Family β—½  
Sequence Similarities

A mesophilic strain, designated A 9500T, was isolated from a soil sample collected from Sobaek Mountain, South Korea, and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew well on different ISP media and no diffusible pigments were produced. The optimum temperature for growth was 32β€…Β°C. The aerial mycelium was well developed, but not fragmented. The strain was Gram-positive, non-motile and formed endospores on vegetative and aerial hyphae with a spiny surface. Cell walls of strain A 9500T contained meso-diaminopimelic acid as the diagnostic amino acid, but no characteristic sugars or other amino acids were found (chemotype-III). The major menaquinone was MK-9(H4) and the minor menaquinone was MK-10(H4) and they were detected at a ratio of 7β€Š:β€Š3. Phosphatidylethanolamine was the diagnostic phospholipid. The G+C content of the genomic DNA was 39.4β€…mol%. The major fatty acids were anteiso-C15β€Š:β€Š0 (43.34β€Š%), iso-C16β€Š:β€Š0 (14.23β€Š%) and C16β€Š:β€Š0 (7.90β€Š%), a composition that differed from members of related genera of the family Thermoactinomycetaceae. The novel strain formed a distinct clade in a phylogenetic tree based on 16S rRNA gene sequences. On the basis of a range of phenotypic and genotypic data and on 16S rRNA gene sequence similarities (88.35–90.38β€Š%), it is suggested that strain A 9500T represents a novel species in a new genus with the name Shimazuella kribbensis gen. nov., sp. nov. The type strain of the type species is A 9500T (=KCTC 9933T=DSM 45090T).

Jinshanibacter, a new genus of Budviciaceae: identification of Jinshanibacter zhutongyuii sp. nov. and Jinshanibacter xujianqingii sp. nov. isolated from cloacal content of snow finch (Montifringilla taczanowskii)

2021 β—½  
Vol 71 (2) β—½  
Author(s):  
Yajun Ge β—½  
Bin Wang β—½  
Jing Yang β—½  
Xin-He Lai β—½  
Gui Zhang β—½  
...  
Keyword(s):  
Type Strain β—½  
Type Species β—½  
New Genus β—½  
Major Fatty Acid β—½  
Phylogenomic Analysis β—½  
Rrna Gene β—½  
Phenotypic Properties β—½  
Content Type β—½  
Link Type β—½  
The Family

Four novel strains isolated from the cloacal contents of snow finches (Montifringilla taczanowskii) were characterized as aerobic, Gram-stain-negative, slightly motile, and rod-shaped. Analysis of the 16S rRNA gene sequence revealed that strain CF-458T had the highest similarities of 96.9 and 96.4β€Š% with Limnobaculum parvum HYN0051T and Pragia fontium DSM 5563T, while strain CF-1111T shared the highest similarities of 96.4 and 96.1β€Š% with Pantoea rodasii LMG 26273T and Pectobacterium punjabense SS95T. Phylogenomic analysis showed the four isolates were separated into group β…  (CF-458T and CF-917) and group β…‘ (CF-1111T and CF-509), and clustered independently in the vicinity of the genera Limnobaculum and Pragia . Summed feature 3 (C16β€Š:β€Š1  ω7c and/or C16β€Š:β€Š1  ω6c, 23.9 and 17.2β€Š%, respectively), C16β€Š:β€Š0 (21.8 and 22.1β€Š%, respectively) and C14β€Š:β€Š0 (10.6 and 17.7β€Š%, respectively) were the common major fatty acids, and summed feature 8 (C18β€Š:β€Š1  ω7c and/or C18β€Š:β€Š1  ω6c, 12.3β€Š%) was also a major fatty acid for strain CF-458T while cyclo-C17β€Š:β€Š0 (13.1%) was for strain CF-1111T. Both had Q-8 as the sole quinone and contained phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as the major polar lipids. The DNA G+C content of strains CF-458T and CF-1111T was 45.7 and 45.4 mol%, respectively. Based on taxonomic position in the phylogenomic tree and phenotypic properties, two novel species of a new genus within the family Budviciaceae are thus proposed, with the name Jinshanibacter gen. nov., zhutongyuii sp. nov. (type strain CF-458T=CGMCC 1.16483T=GDMCC 1.1586T=JCM 33489T) and Jinshanibacter xujianqingii sp. nov. (type strain CF-1111T=CGMCC 1.16786T=GDMCC 1.1587T=JCM 33490T), respectively.

scholarly journals Thermotunica guangxiensis gen. nov., sp. nov., isolated from mushroom residue compost

2014 β—½  
Vol 64 (Pt_5) β—½  
pp. 1593-1599 β—½  
Author(s):  
Hao Wu β—½  
Yunpeng Lian β—½  
Bin Liu β—½  
Yanling Ren β—½  
Peisheng Qin β—½  
...  
Keyword(s):  
Aerial Mycelium β—½  
Surface Strain β—½  
Diaminopimelic Acid β—½  
Rrna Gene β—½  
Membranous Structure β—½  
Content Type β—½  
Link Type β—½  
The Family β—½  
Diamino Acid β—½  
Family Based

A novel thermophilic actinomycete, designated AG2-7T, was isolated from mushroom residue compost in Guangxi University, Nanning, China. The strain grew optimally at 45–60 Β°C, at pH 7.0 and with 0–3.0β€Š% (w/v) NaCl. Vegetative mycelia were branched and whitish to pale yellow without fragmentation. Aerial mycelium was abundant, whitish and differentiated into long chains of spores, with a membranous structure or tunica partially covering the surface of aerial hyphae. The non-motile spores were oval in shape with a ridged surface. Strain AG-27T contained meso-diaminopimelic acid as the diagnostic diamino acid, and the whole-cell sugars were galactose and ribose. Major fatty acids were iso-C16β€Š:β€Š0 (27.51β€Š%), iso-C17β€Š:β€Š0 (10.47β€Š%) and anteiso-C17β€Š:β€Š0 (12.01β€Š%). MK-9(H4) was the predominant menaquinone. The polar phospholipids were diphosphatidylglycerol, ninhydrin-positive glycophospholipid, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylethanolamine, phosphatidylmethylethanolamine, an unknown phospholipid and unknown glucosamine-containing phospholipids. The G+C content of the genomic DNA was 63.6 mol%. 16S rRNA gene sequence analysis showed that the organism belonged to the family Pseudonocardiaceae , suborder Pseudonocardineae and showed more than 5β€Š% divergence from other members of the family. Based on the phenotypic and phylogenetic data, strain AG2-7T represents a novel species of a new genus in the family Pseudonocardiaceae , for which the name Thermotunica guangxiensis gen. nov., sp. nov. is proposed. The type strain of the type species is AG2-7T (β€Š=β€ŠATCC BAA-2499Tβ€Š=β€ŠCGMCC 4.7099T).

scholarly journals Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea

2007 β—½  
Vol 57 (4) β—½  
pp. 687-691 β—½  
Author(s):  
Ying-Shun Cui β—½  
Wan-Taek Im β—½  
Cheng-Ri Yin β—½  
Jung-Sook Lee β—½  
Keun Chul Lee β—½  
...  
Keyword(s):  
South Korea β—½  
Type Strain β—½  
Sequence Similarity β—½  
Diaminopimelic Acid β—½  
Rrna Gene β—½  
Strictly Aerobic β—½  
Aerobic Bacterium β—½  
The Novel β—½  
Biochemical Tests β—½  
The Family

A Gram-positive, rod-shaped, non-spore-forming and strictly aerobic bacterium (Gsoil 161T) was isolated from soil of a ginseng field in Pocheon Province, South Korea. The novel isolate was characterized using a polyphasic approach in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 161T was shown to belong to the family Nocardioidaceae and was related to Aeromicrobium marinum (98.0β€Š% similarity to the type strain), Aeromicrobium alkaliterrae (97.6β€Š%), Aeromicrobium fastidiosum (97.0β€Š%) and Aeromicrobium erythreum (96.7β€Š%); the sequence similarity with other species within the family was less than 94.4β€Š%. It was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H4) as the predominant menaquinone and C16β€Š:β€Š0, 10-methyl C18β€Š:β€Š0 (tuberculostearic acid), C16β€Š:β€Š0 2-OH, 10-methyl C17β€Š:β€Š0 and 10-methyl-C16β€Š:β€Š0 as the major fatty acids. The G+C content of the genomic DNA was 65.5β€…mol%. These chemotaxonomic properties and phenotypic characteristics support the affiliation of strain Gsoil 161T to the genus Aeromicrobium. Results of physiological and biochemical tests enabled strain Gsoil 161T to be differentiated genotypically and phenotypically from currently known Aeromicrobium species. Therefore, strain Gsoil 161T represents a novel species, for which the name Aeromicrobium panaciterrae sp. nov. is proposed. The type strain is strain Gsoil 161T (=KCTC 19131T=DSM 17939T=CCUG 52476T).

scholarly journals Pelagicoccus croceus sp. nov., a novel marine member of the family Puniceicoccaceae within the phylum β€˜Verrucomicrobia’ isolated from seagrass

2007 β—½  
Vol 57 (12) β—½  
pp. 2874-2880 β—½  
Author(s):  
Jaewoo Yoon β—½  
Naoya Oku β—½  
Satoru Matsuda β—½  
Hiroaki Kasai β—½  
Akira Yokota
Keyword(s):  
Cell Wall β—½  
Novel Species β—½  
Sequence Similarity β—½  
Phylogenetic Analyses β—½  
Diaminopimelic Acid β—½  
Rrna Gene β—½  
The Novel β—½  
Antibiotic Susceptibility Test β—½  
The Family β—½  
Lactam Antibiotic

An obligately aerobic, spherical, non-motile, pale-yellow pigmented bacterium was isolated from a piece of leaf of seagrass, Enhalus acoroides (L.f.) Royle, grown in Okinawa, Japan and was subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolate N5FB36-5T shared approximately 96–98β€Š% sequence similarity with the species of the genus Pelagicoccus of the family Puniceicoccaceae within the phylum β€˜Verrucomicrobia’. The DNA–DNA relatedness values of strain N5FB36-5T with Pelagicoccus mobilis 02PA-Ca-133T and Pelagicoccus albus YM14-201T were below 70β€Š%, which is accepted as the phylogenetic definition of a novel species. Ξ²-Lactam antibiotic susceptibility test and amino acid analysis of the cell wall hydrolysates indicated the absence of muramic acid and diaminopimelic acid in the cell walls, which suggested that this strain lacks an ordinary Gram-negative type of peptidoglycan in the cell wall. The DNA G+C content of strain N5FB36-5T was 51.6β€…mol%; MK-7 was the major menaquinone; and the presence of C16β€Š:β€Š0, C16β€Š:β€Š1 Ο‰7c and anteiso-C15β€Š:β€Š0 as the major cellular fatty acids supported the identification of the novel isolate as a member of the genus Pelagicoccus. On the basis of polyphasic taxonomic data, it was concluded that this strain should be classified as a novel species of the genus Pelagicoccus, for which the name Pelagicoccus croceus sp. nov. is proposed. The type strain is N5FB36-5T (=MBIC08282T=KCTC 12903T).

scholarly journals Nocardioides ginsengagri sp. nov., isolated from the soil of a ginseng field

2012 β—½  
Vol 62 (Pt_3) β—½  
pp. 591-595 β—½  
Author(s):  
Sang-Hee Lee β—½  
Qing-Mei Liu β—½  
Sung-Taik Lee β—½  
Sun-Chang Kim β—½  
Wan-Taek Im
Keyword(s):  
Gene Sequence β—½  
Sequence Similarity β—½  
Phylogenetic Analyses β—½  
Diaminopimelic Acid β—½  
Rrna Gene β—½  
The Novel β—½  
Phenotypic Data β—½  
Gene Sequence Analysis β—½  
The Family β—½  
Bacterium Strain

A Gram-reaction-positive, rod-shaped, non-motile, non-spore-forming bacterium (strain BX5-10T) was isolated from the soil of a ginseng field on Baekdu Mountain in Jilin district, China. The taxonomic position of this bacterium was determined in an investigation based on a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain BX5-10T was shown to belong to the family Nocardioidaceae and to be most closely related to Nocardioides plantarum NCIMB 12834T (96.5β€Š% sequence similarity), Nocardioides dokdonensis KCTC 19309T (96.2β€Š%) and Nocardioides fonticola NAA-13T (95.1β€Š%). Strain BX5-10T was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in its cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and C18β€Š:β€Š1Ο‰9c, C16β€Š:β€Š0 and C17β€Š:β€Š1Ο‰8c as its major fatty acids. The G+C content of the genomic DNA was 70.3 mol%. The novel strain could be differentiated genotypically and phenotypically from all recognized species of the genus Nocardioides. Based on the results of the phylogenetic analyses and the genotypic and phenotypic data, a novel species, Nocardioides ginsengagri sp. nov., is proposed. The type strain is BX5-10T (β€Š=β€ŠKCTC 19467Tβ€Š=β€ŠDSM 21362T).

scholarly journals Auraticoccus monumenti gen. nov., sp. nov., an actinomycete isolated from a deteriorated sandstone monument

2011 β—½  
Vol 61 (5) β—½  
pp. 1098-1103 β—½  
Author(s):  
Pablo Alonso-Vega β—½  
Lorena Carro β—½  
Eustoquio Martínez-Molina β—½  
Martha E. Trujillo
Keyword(s):  
New Genus β—½  
Gene Sequence β—½  
Diaminopimelic Acid β—½  
Physiological Data β—½  
Rrna Gene β—½  
Strictly Aerobic β—½  
The Public β—½  
The Family β—½  
Chemotaxonomic Markers

A Gram-type-positive, strictly aerobic actinobacterium, designated strain MON 2.2T, was isolated from the surface of a sandstone monument. Cells with a coccoid shape, arranged in pairs or clusters, were non-motile and did not produce spores. The 10 closest 16S rRNA gene sequence matches (~95β€Š% similarity) found in the public databases were uncultured actinobacteria, while the closest cultured members indicated a phylogenetic relationship with members of the family Propionibacteriaceae (92–95β€Š% similarity). Subsequent phylogenetic analysis placed the new isolate within the radiation of the genera Friedmanniella and Microlunatus, but forming an independent branch. Chemotaxonomic markers were consistent with the classification of strain MON 2.2T in the family Propionibacteriaceae, amongst the genera containing ll-diaminopimelic acid in their peptidoglycan. Characteristic fatty acids iso-C15β€Š:β€Š0 and anteiso-C15β€Š:β€Š0 also supported its affiliation to this taxon; however, polar lipid and menaquinone compositions clearly differentiated strain MON 2.2T from other genera in the family. On the basis of these results and additional physiological data obtained in the present study, it is proposed that strain MON 2.2T be classified in a novel species in a new genus, for which the name Auraticoccus monumenti gen. nov., sp. nov. is proposed. The type strain of Auraticoccus monumenti is MON 2.2T (β€Š=β€ŠCECT 7672T β€Š=β€ŠDSM 23257T β€Š=β€ŠLMG 25551T).

scholarly journals Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov.

2011 β—½  
Vol 61 (3) β—½  
pp. 518-523 β—½  
Author(s):  
P. Kämpfer β—½  
N. Lodders β—½  
E. Falsen
Keyword(s):  
New Genus β—½  
Novel Species β—½  
Sequence Similarity β—½  
Rrna Gene β—½  
The Novel β—½  
Fatty Acid Profiles β—½  
Bacterial Strains β—½  
16S Rrna Gene Sequences β—½  
Biochemical Tests β—½  
The Family

Three bacterial strains, designated CCUG 51397T, CCUG 53736 and CCUG 53920, isolated from water samples taken at different locations in southern Sweden were studied to determine their taxonomic position using a polyphasic approach. Comparative analysis of 16S rRNA gene sequences showed that these bacteria had <93β€Š% sequence similarity to all described species of the genera Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Chitinophaga. The three organisms grouped most closely with Sediminibacterium salmoneum NJ-44T but showed only 92.5β€Š% sequence similarity to this strain, the only recognized species of this genus. The fatty acid profiles showed large amounts of iso-C15β€Š:β€Š0, iso-C17β€Š:β€Š0 3-OH and iso-C15β€Š:β€Š1 G with smaller amounts of iso-C15β€Š:β€Š0 3-OH, iso-C16β€Š:β€Š0 3-OH and other fatty acids, which differentiated the novel strains from related genera. Biochemical tests performed on strains CCUG 51397T, CCUG 53736 and CCUG 53920 also gave different results from those of Sediminibacterium salmoneum NJ-44T and other related genera. Based on this evidence, strains CCUG 51397T, CCUG 53736 and CCUG 53920 represent a novel species of a new genus, for which the name Hydrotalea flava gen. nov., sp. nov. is proposed. The type strain of Hydrotalea flava is CCUG 51397T (=CCM 7760T). A formal allocation of the genera Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Chitinophaga to the family Chitinophagaceae fam. nov. is also proposed.

scholarly journals Humihabitans oryzae gen. nov., sp. nov.

2007 β—½  
Vol 57 (9) β—½  
pp. 2163-2166 β—½  
Author(s):  
Akiko Kageyama β—½  
Yoko Takahashi β—½  
Satoshi Ōmura
Keyword(s):  
New Genus β—½  
Diaminopimelic Acid β—½  
Rrna Gene β—½  
New Genus And Species β—½  
Mycolic Acids β—½  
Gene Sequence Analysis β—½  
The Family β—½  
Paddy Field Soil β—½  
Diamino Acid β—½  
Aerobic Organism

Strain KV-657T was isolated from a paddy field soil sample collected in Japan using GPM agar plates supplemented with catalase. The strain was a Gram-positive, aerobic organism that formed branching hyphae with ll-diaminopimelic acid as the diagnostic peptidoglycan diamino acid. The major menaquinone was MK-8(H4). Mycolic acids were not detected. The G+C content of the DNA was 70β€…mol%. 16S rRNA gene sequence analysis revealed that this strain is closely related to Intrasporangium calvum DSM 43043T, with a similarity of 97.6β€Š%. Based on the morphological, biochemical and chemotaxonomic properties of this strain and phylogenetic analysis, it was concluded that this isolate represents a new genus and species in the family Intrasporangiaceae, for which the name Humihabitans oryzae gen. nov., sp. nov. is proposed. The type strain of Humihabitans oryzae is KV-657T (=NRRL B-24470T =NBRC 101802T).

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