scholarly journals Maribacter flavus sp. nov., isolated from a cyanobacterial culture pond

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 3997-4002 ◽  
Author(s):  
Mingxing Tang ◽  
Guanghua Wang ◽  
Wenzhou Xiang ◽  
Chenghao Chen ◽  
Jiayi Wu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Cyanobacterial Culture ◽  
The Family ◽  
Optimum Ph ◽  
Total Fatty Acids ◽  
Distinct Lineage ◽  
Type Strains

A Gram-stain-negative, yellow, non-spore-forming, strictly aerobic bacterium, designated C3T, was isolated from a cyanobacterial culture pond. Cells were halophilic, rod-shaped and able to move by gliding. Growth of strain C3T was observed at 15–30 °C (optimum 25 °C), pH 6.0–9.0 (optimum pH 7.5), and in the presence of 1–7 % (w/v) NaCl (optimum 2–3 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain C3T formed a distinct lineage within the family Flavobacteriaceae and exhibited the highest similarity (95.21 %) to the type strains of Maribacter dokdonensis, Maribacter arcticus, Maribacter orientalis and Maribacter stanieri, and ‘Maribacter caenipelagi’ HD-44. The only isoprenoid quinone present within strain C3T was menaquinone 6 (MK-6). The G+C content of genomic DNA was 41.5 mol%. The major polar lipids were phosphatidylethanolamine and three unidentified lipids. The predominant cellular fatty acids (>5 % of the total fatty acids) were iso-C15 : 1 G, iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain C3T represents a novel species of the genus Maribacter, for which the name Maribacter flavus sp. nov. is proposed. The type strain is C3T ( = KCTC 42508T = CGMCC 1.15112T).

scholarly journals Gillisia myxillae sp. nov., a novel member of the family Flavobacteriaceae, isolated from the marine sponge Myxilla incrustans

2006 ◽  
Vol 56 (8) ◽  
pp. 1795-1799 ◽  
Author(s):  
On On Lee ◽  
Stanley C. K. Lau ◽  
Mandy M. Y. Tsoi ◽  
Xiancui Li ◽  
Ioulia Plakhotnikova ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Marine Sponge ◽  
Phenotypic Traits ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Respiratory Quinone ◽  
Total Fatty Acids ◽  
Bacterium Strain

A yellow-pigmented, Gram-negative, rod-shaped, strictly aerobic bacterium (strain UST050418-085T) was isolated from the surface of a marine sponge, Myxilla incrustans, at Friday Harbor, WA, USA. The DNA G+C content of this strain was 34.6 mol%. The predominant fatty acids were i15 : 0, a15 : 0, i15 : 1, i16 : 0, i17 : 0 3-OH, 17 : 0 2-OH and summed feature 3, comprising i15 : 0 2-OH and/or 16 : 1ω7c (altogether representing 69.0 % of the total fatty acids). MK-6 was the only respiratory quinone detected. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of UST050418-085T were members of the genus Gillisia, with sequence similarities of 93.2–96.6 %. Strain UST050418-085T differed from its closest relatives by 11 to 18 phenotypic traits. Molecular evidence and phenotypic characteristics suggest that strain UST050418-085T represents a novel species within the genus Gillisia. The name Gillisia myxillae sp. nov. is proposed, with UST050418-085T (=JCM 13546T=NRRL B-41416T) as the type strain.

scholarly journals Tenacibaculum dicentrarchi sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from European sea bass

2012 ◽  
Vol 62 (2) ◽  
pp. 425-429 ◽  
Author(s):  
Maximino Piñeiro-Vidal ◽  
Daniel Gijón ◽  
Carles Zarza ◽  
Ysabel Santos
Keyword(s):  
Fatty Acids ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Rrna Gene ◽  
European Sea Bass ◽  
Phenotypic Data ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Total Fatty Acids ◽  
Type Strains

A novel Gram-stain-negative rod-shaped gliding bacterial strain, designated 35/09T, was isolated from diseased European sea bass (Dicentrarchus labrax L.) in Spain. Colonies were pale-yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the isolate was 31.3 mol%. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum (family Flavobacteriaceae, phylum ‘Bacteroidetes’). Sequence similarities between the isolate and type strains of other members of the genus were 93.1–97.3 %. The major fatty acids (>5 % of the total fatty acids) were iso-C15 : 0 (24.8 %), iso-C15 : 0 3-OH (18.0 %), anteiso-C15 : 0 (8.1 %), C15 : 1ω6c (6.9 %) and iso-C15 : 1 (6.2 %). Genotypic and phenotypic data indicate that strain 35/09T should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum dicentrarchi sp. nov. is proposed; the type strain is 35/09T ( = CECT 7612T = NCIMB 14598T).

scholarly journals Nonlabens tegetincola gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from a microbial mat in a subtropical estuary

2005 ◽  
Vol 55 (6) ◽  
pp. 2279-2283 ◽  
Author(s):  
Stanley C. K. Lau ◽  
Mandy M. Y. Tsoi ◽  
Xiancui Li ◽  
Ioulia Plakhotnikova ◽  
Sergey Dobretsov ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Microbial Mat ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
The Bahamas ◽  
Respiratory Quinone ◽  
The Family ◽  
Distinct Lineage ◽  
Subtropical Estuary

An orange-pigmented, Gram-negative, non-motile, rod-shaped, strictly aerobic bacterium (UST030701-324T) was isolated from a microbial mat in an estuary in the Bahamas. The DNA G+C content was 33·6 mol%. Predominant fatty acids were i15 : 0, i16 : 0, i17 : 0 3-OH, and summed feature 3, comprising i15 : 0 2-OH and/or 16 : 1ω7c. MK-6 was the only respiratory quinone. Flexirubin-type pigments were not produced. Phylogenetic analysis based on 16S rRNA gene sequences placed UST030701-324T in a distinct lineage in the family Flavobacteriaceae with less than 90·7 % sequence similarity to the members of the nearest genus, Psychroflexus. UST030701-324T can be distinguished from other members of Flavobacteriaceae by a number of chemotaxonomic and phenotypic characteristics. It is thus proposed that UST030701-324T represents a novel taxon designated Nonlabens tegetincola gen. nov., sp. nov. The type strain is UST030701-324T (=NRRL B-41136T=JCM 12886T).

scholarly journals Rheinheimera aestuari sp. nov., a marine bacterium isolated from coastal sediment

2015 ◽  
Vol 65 (Pt_8) ◽  
pp. 2640-2645 ◽  
Author(s):  
Kyunghwa Baek ◽  
Che Ok Jeon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Analyses ◽  
Coastal Sediment ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Family ◽  
Optimum Ph ◽  
Type Strains ◽  
Sequence Similarities

A Gram-stain-negative, strictly aerobic, non-pigmented, motile bacterium with a single polar flagellum, designated H29T, was isolated from coastal sediment of Jeju Island, South Korea. Cells were non-spore-forming rods showing catalase- and oxidase-positive reactions. Growth of strain H29T was observed at 10–40 °C (optimum, 20–25 °C) and pH 6.0–9.0 (optimum, pH 7.0–8.0), and in the presence of 1–4 % (w/v) NaCl (optimum, 2–3 %). Strain H29T contained C16 : 0, iso-C15 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c) as the major fatty acids and ubiquinone-8 (Q-8) as the sole isoprenoid quinone. Phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids. The G+C content of the genomic DNA was 46.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain H29T formed a phyletic lineage with Rheinheimera hassiensis E48T within the genus Rheinheimera of the family Chromatiaceae. Strain H29T was most closely related to Rheinheimera pacifica KMM 1406T, Rheinheimera muenzenbergensis E49T, Rheinheimera hassiensis E48T and Rheinheimera baltica OSBAC1T with 97.8 %, 97.6 %, 97.4 % and 97.2 % 16S rRNA gene sequence similarities, respectively. However, DNA–DNA hybridization values of strain H29T with type strains of these species were lower than 70 %. On the basis of the phenotypic, chemotaxonomic and molecular properties, strain H29T represents a novel species of the genus Rheinheimera, for which the name Rheinheimera aestuarii sp. nov. is proposed. The type strain is H29T ( = KACC 18251T = JCM 30404T).

scholarly journals Lutibacter holmesii sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Lutibacter

2015 ◽  
Vol 65 (Pt_11) ◽  
pp. 3991-3996 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Stefanie Van Trappen ◽  
Natalia V. Zhukova ◽  
Paul De Vos
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sea Urchin ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Strongylocentrotus Intermedius ◽  
The Family ◽  
Type Strains

Seven Gram-staining-negative, strictly aerobic, pale-yellow-pigmented, rod-shaped and non-motile strains were isolated from the sea urchin Strongylocentrotus intermedius collected from Troitsa Bay, Sea of Japan. Phylogenetic analyses based on 16S rRNA gene sequences showed that these isolates were affiliated with the family Flavobacteriaceae. The novel isolates showed 99.9–100 % 16S rRNA gene sequence similarity to each other and were closely related to the type strains of the recognized members of the genus Lutibacter with sequence similarities of 95.8–98.4 %. The G+C content of the genomic DNA was 35–36 mol%. DNA–DNA relatedness among the sea urchin isolates was 95–99 % and between strain KMM 6277T and its most closely related type strains, Lutibacter agarilyticus KCTC 23842T and Lutibacter litoralis JCM 13034T, was 38 and 27 %, respectively. The prevalent fatty acids were iso-C15 : 0, anteiso-C15 : 0, summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1 ω7c fatty acids), iso-C15 : 1 and C15 : 0. The polar lipid profile was composed of the phosphatidylethanolamine, one unknown aminolipid and one unknown lipid. The main respiratory isoprenoid quinone was MK-6.The results of phylogenetic, phenotypic and genotypic analyses indicated that the novel strains represent a novel species within the genus Lutibacter, for which the name Lutibacter holmesii sp. nov. is proposed. The type strain is KMM 6277T ( = CCUG 62221T = LMG 26737T).

scholarly journals Nocardioides panacihumi sp. nov., isolated from soil of a ginseng field

2007 ◽  
Vol 57 (9) ◽  
pp. 2143-2146 ◽  
Author(s):  
Dong-Shan An ◽  
Wan-Taek Im ◽  
Sung-Taik Lee ◽  
Min-Ho Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
The Family ◽  
Established Species ◽  
Type Strains

A novel bacterial strain designated Gsoil 616T was isolated from a soil sample of a ginseng field in Pocheon province (South Korea) and was characterized taxonomically by using a polyphasic approach. The isolate was Gram-positive, strictly aerobic, non-motile, non-spore-forming and rod- or coccoid-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Nocardioides in the family Nocardioidaceae but was clearly separated from established species of this genus. The 16S rRNA gene sequence similarities between strain Gsoil 616T and the type strains of Nocardioides species with validly published names ranged from 91.8 to 96.1 %. The G+C content of the genomic DNA was 73 mol%. Phenotypic and chemotaxonomic data [major menaquinone MK-8(H4) and major fatty acid iso-C16 : 0] supported the affiliation of strain Gsoil 616T to the genus Nocardioides. However, the results of physiological and biochemical tests allowed phenotypic differentiation of the isolate from other Nocardioides species. Therefore, strain Gsoil 616T represented a novel species within the genus Nocardioides, for which the name Nocardioides panacihumi sp. nov. is proposed. The type strain is Gsoil 616T (=KCTC 19187T =DSM 18660T).

scholarly journals Oceanobacillus arenosus sp. nov., a moderately halophilic bacterium isolated from marine sand

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2943-2948 ◽  
Author(s):  
Wonyong Kim ◽  
Chatuphon Siamphan ◽  
Jong-Hwa Kim ◽  
Ampaitip Sukhoom
Keyword(s):  
Halophilic Bacterium ◽  
Cellular Fatty Acid ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Marine Sand ◽  
Moderately Halophilic Bacterium ◽  
Distinct Lineage ◽  
Lipid Pattern

A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterium, designated CAU 1183T, was isolated from marine sand and its taxonomic position was investigated by using a polyphasic approach. The bacterium grew optimally at 30 °C, at pH 8.5 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1183T formed a distinct lineage within the genus Oceanobacillus and exhibited the highest similarity to Oceanobacillus chungangensis CAU 1051T (97.6 %). The strain contained MK-7 as the predominant isoprenoid quinone and anteiso-C15 : 0 was the major cellular fatty acid. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polar lipid pattern of strain CAU 1183T consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified lipids, including two phospholipids, two glycolipids, a phosphoglycolipid and two lipids. The G+C content of the genomic DNA was 37.5 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain CAU 1183T should be assigned to a novel species in the genus Oceanobacillus, for which the name Oceanobacillus arenosus sp. nov. is proposed. The type strain is CAU 1183T ( = KCTC 33037T = CECT 8560T).

Kaistella flava sp. nov., isolated from Antarctic tundra soil, and emended descriptions of Kaistella yonginensis, Kaistella jeonii, Kaistella antarctica and Kaistella chaponensis

2021 ◽  
Author(s):  
Xiaoya Peng ◽  
Yumin Zhang ◽  
Yijing Lu ◽  
Xueyin Zhou ◽  
Zhourui Wei ◽  
...  
Keyword(s):  
Type Species ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
The Family ◽  
Optimum Ph ◽  
Genome Level ◽  
Sequence Similarities

A rod-shaped, yellow-pigmented, Gram-stain-negative, non-motile and aerobic bacterium, designated 7-3AT, was isolated from soil from King George Island, maritime Antarctica, and subjected to a polyphasic taxonomic study. Growth occurred at 4–37 °C (optimum, 20°C) and at pH 5.0–9.0 (optimum, pH 7.0–8.0). Tolerance to NaCl was up to 4 % (w/v) with optimum growth in the absence of NaCl. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 7-3AT represented a member of the family Flavobacteriaceae . Strain 7-3AT showed the highest sequence similarities with Kaistella yonginensis HMD 1043T (96.65 %), Kaistella carnis NCTC 13525T (96.53 %), Kaistella chaponensis DSM 23145T (96.27 %), Kaistella antarctica LMG 24720T (96.13 %) and Kaistella jeonii DSM 17048T (96.06 %). A whole genome-level comparison of 7-3AT with K. jeonii DSM 17048T, K. antarctica LMG 24720T, K. chaponensis DSM 23145T, and Kaistella palustris DSM 21579T revealed average nucleotide identity (ANI) values of 79.03, 82.25, 78.12, and 74.42 %, respectively. The major respiratory isoprenoid quinone was identified as MK-6 and a few ubiquinones Q-10 were identified. In addition, flexirubin-type pigments were absent. The polar lipid profile of 7-3AT was found to contain one phosphatidylethanolamine, six unidentified aminolipids (AL) and two unidentified lipids (L). The G+C content of the genomic DNA was determined to be 34.54 mol%. The main fatty acids were iso-C15 : 0, summed feature 9 (comprising iso-C17 : 1ω9c and/or C16 : 0 10-methyl), anteiso-C15 : 0, iso-C13 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). On the basis of the evidence presented in this study, a novel species of the genus Kaistella , Kaistella flava sp. nov., is proposed, with the type strain 7-3AT (=CCTCC AB 2016141T= KCTC 52492T). Emended descriptions of Kaistella yonginensis , Kaistella jeonii , Kaistella antarctica and Kaistella chaponensis are also given.

scholarly journals Rapidithrix thailandica gen. nov., sp. nov., a marine gliding bacterium isolated from samples collected from the Andaman sea, along the southern coastline of Thailand

2007 ◽  
Vol 57 (10) ◽  
pp. 2275-2279 ◽  
Author(s):  
Pornpoj Srisukchayakul ◽  
Chatrudee Suwanachart ◽  
Yutthapong Sangnoi ◽  
Akkharawit Kanjana-Opas ◽  
Shoichi Hosoya ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Andaman Sea ◽  
Rrna Gene ◽  
Hexadecenoic Acid ◽  
Respiratory Quinone ◽  
The Family ◽  
Distinct Lineage ◽  
Major Respiratory Quinone ◽  
Hydroxyl Fatty Acids ◽  
Gliding Bacterium

The taxonomic positions of three strains of marine gliding bacteria, TISTR 1736, TISTR 1741 and TISTR 1750T, isolated from the southern coastline of Thailand were evaluated by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the three isolates formed a distinct lineage within the family ‘Flammeovirgaceae’, phylum Bacteroidetes, and were related to the genus Flexithrix. The DNA G+C contents of the isolates were in the range 40–43 mol%. The major respiratory quinone was MK-7. The major cellular fatty acids were 16 : 1ω5c (cis-5-hexadecenoic acid) and 15 : 0 (pentadecanoic acid). The major hydroxyl fatty acids were 3-OH 17 : 0 (3-hydroxyheptadecanoic acid), 3-OH 15 : 0 (3-hydroxypentadecanoic acid) and 3-OH 16 : 0 (3-hydroxyhexadecanoic acid). On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic data, these marine bacteria are considered to represent a novel species of a new genus, for which the name Rapidithrix thailandica gen. nov., sp. nov. is proposed. The type strain of Rapidithrix thailandica is TISTR 1750T (=IAM 15448T).

scholarly journals Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea

2007 ◽  
Vol 57 (4) ◽  
pp. 687-691 ◽  
Author(s):  
Ying-Shun Cui ◽  
Wan-Taek Im ◽  
Cheng-Ri Yin ◽  
Jung-Sook Lee ◽  
Keun Chul Lee ◽  
...  
Keyword(s):  
South Korea ◽  
Type Strain ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
The Novel ◽  
Biochemical Tests ◽  
The Family

A Gram-positive, rod-shaped, non-spore-forming and strictly aerobic bacterium (Gsoil 161T) was isolated from soil of a ginseng field in Pocheon Province, South Korea. The novel isolate was characterized using a polyphasic approach in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 161T was shown to belong to the family Nocardioidaceae and was related to Aeromicrobium marinum (98.0 % similarity to the type strain), Aeromicrobium alkaliterrae (97.6 %), Aeromicrobium fastidiosum (97.0 %) and Aeromicrobium erythreum (96.7 %); the sequence similarity with other species within the family was less than 94.4 %. It was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H4) as the predominant menaquinone and C16 : 0, 10-methyl C18 : 0 (tuberculostearic acid), C16 : 0 2-OH, 10-methyl C17 : 0 and 10-methyl-C16 : 0 as the major fatty acids. The G+C content of the genomic DNA was 65.5 mol%. These chemotaxonomic properties and phenotypic characteristics support the affiliation of strain Gsoil 161T to the genus Aeromicrobium. Results of physiological and biochemical tests enabled strain Gsoil 161T to be differentiated genotypically and phenotypically from currently known Aeromicrobium species. Therefore, strain Gsoil 161T represents a novel species, for which the name Aeromicrobium panaciterrae sp. nov. is proposed. The type strain is strain Gsoil 161T (=KCTC 19131T=DSM 17939T=CCUG 52476T).

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