scholarly journals Micromonospora zhanjiangensis sp. nov., isolated from mangrove forest soil

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4880-4885 ◽  
Author(s):  
Li Zhang ◽  
Lei Li ◽  
Zixin Deng ◽  
Kui Hong
Keyword(s):  
Dna Hybridization ◽  
Mangrove Forest ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Hybridization Data ◽  
Reference Strains ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

A novel actinomycete, designated strain 2902at01T was isolated from soil collected at a mangrove forest in Zhanjiang, Guangdong province, China. The strain was identified using a polyphasic classification method. The 16S rRNA gene sequence of strain 2902at01T showed the highest similarity to Micromonospora equina Y22T (98.3 %) and Micromonospora pattaloongensis TJ2-2T (98.1 %). Phylogenetic analysis based on the gyrB gene sequence also clearly showed that the strain was different from any previously discovered species of the genus Micromonospora. The characteristic whole-cell sugars were ribose and xylose. The cell-wall hydrolysates contained alanine, asparagine, glycine and meso-diaminopimelic acid. MK-10(H6) and MK-10(H8) were the major menaquinones of the novel strain. The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0. The characteristic polar lipids of strain 2902at01T were phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and diphosphatidylglycerol. The DNA G+C content was 70.2 mol%. DNA–DNA hybridization data combined with other physiological and biochemical features could distinguish strain 2902at01T from the reference strains M. equina Y22T and M. pattaloongensis TJ2-2 T. On the basis of these phenotypic and genotypic data, strain 2902at01T represents a novel species of the genus Micromonospora, for which the name Micromonospora zhanjiangensis sp. nov. is proposed. The type strain is 2902at01T ( = CCTCC AA2014018T = DSM 45902T).

scholarly journals Streptomyces cheonanensis sp. nov., a novel streptomycete with antifungal activity

2006 ◽  
Vol 56 (2) ◽  
pp. 471-475 ◽  
Author(s):  
Hyo Jin Kim ◽  
Sung Chul Lee ◽  
Byung Kook Hwang
Keyword(s):  
Antifungal Activity ◽  
Gene Sequence ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Physiological And Biochemical Characteristics ◽  
Actinomycete Strain ◽  
Reference Strains ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

Actinomycete strain VC-A46T, which exhibits antifungal activity, was isolated from a soil sample collected from Cheonan, Korea. The strain was determined to belong to the genus Streptomyces, based on its morphological and chemotaxonomic characteristics. The cell wall of this strain contained ll-diaminopimelic acid. The predominant fatty acids were iso-C16 : 0, C16 : 0 and C16 : 1 cis 9. The DNA G+C content of strain VC-A46T was 75·5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence of this strain revealed that it differed from those of the described streptomycetes available in public databases. Analyses of DNA–DNA relatedness data, together with data regarding the strain's morphological, physiological and biochemical characteristics, also confirmed that this strain constitutes a novel Streptomyces taxon, which is distinguishable from closely related reference strains. Therefore, it is proposed that strain VC-A46T (=KCCM 42119T=NBRC 100940T) represents the type strain of a novel species, named Streptomyces cheonanensis sp. nov.

scholarly journals Streptomyces koyangensis sp. nov., a novel actinomycete that produces 4-phenyl-3-butenoic acid

2005 ◽  
Vol 55 (1) ◽  
pp. 257-262 ◽  
Author(s):  
Jee Yeon Lee ◽  
Jung Yeop Lee ◽  
Ho Won Jung ◽  
Byung Kook Hwang
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Random Amplified Polymorphic Dna ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Physiological And Biochemical Characteristics ◽  
Butenoic Acid ◽  
Reference Strains ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

A 4-phenyl-3-butenoic acid-producing actinomycete, designated strain VK-A60T, was isolated from a soil sample collected from Koyang, Korea. Morphological and chemical characteristics of the strain were consistent with those of the genus Streptomyces. The cell wall of the strain contains ll-diaminopimelic acid. The predominant fatty acids are anteiso-C15 : 0, iso-C16 : 0 and C16 : 0. The strain formed a distinct monophyletic line within the 16S rRNA gene sequence phylogenetic tree. Analyses of its morphological, physiological and biochemical characteristics, together with random amplified polymorphic DNA and DNA–DNA relatedness data, confirmed that strain VK-A60T represents a novel Streptomyces taxon that is distinguishable from closely related reference strains. Strain VK-A60T (=KCCM 10555T=NBRC 100598T) is proposed as the type strain of a novel species, for which the name Streptomyces koyangensis sp. nov. is proposed.

scholarly journals Streptomyces fenghuangensis sp. nov., isolated from seawater

2011 ◽  
Vol 61 (12) ◽  
pp. 2811-2815 ◽  
Author(s):  
Honghui Zhu ◽  
Shumei Jiang ◽  
Qing Yao ◽  
Yonghong Wang ◽  
Meibiao Chen ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Substrate Mycelium ◽  
Physiological And Biochemical Characteristics ◽  
Type Strains ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

An actinomycete, designated strain GIMN4.003T, was isolated from seawater collected in Sanya, China. It produced white aerial mycelium and yellow substrate mycelium on Gause’s synthetic agar medium no. 1. The substrate mycelium colour was not sensitive to pH. Scanning electron microscopy observations revealed that GIMN4.003T produced straight to flexuous spore chains of rough to warty spores. ll-Diaminopimelic acid was present in the cell-wall hydrolysate. Based on chemotaxonomy and morphological features, strain GIMN4.003T was identified as a member of the genus Streptomyces. Melanin was not produced. No antimicrobial activity was detected against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Penicillium citrinum or Candida albicans. Analysis of the 16S rRNA gene sequence revealed that the highest sequence similarity was to Streptomyces radiopugnans R97T (99.0 %). However, DNA relatedness between GIMN4.003T and S. radiopugnans DSM 41901T was low (41.24±1.47 %). Furthermore, the morphological, physiological and biochemical characteristics of strain GIMN4.003T were different from those of S. radiopugnans DSM 41901T and the type strains of other closely related Streptomyces species. On the basis of its physiological and molecular properties, it is evident that strain GIMN4.003T ( = CCTCCM 208215T  = NRRL B-24801T) represents the type strain of a novel species within the genus Streptomyces, for which the name Streptomyces fenghuangensis sp. nov. is proposed.

scholarly journals Streptomyces graminifolii sp. nov., isolated from bamboo (Sasa borealis) litter

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2517-2521 ◽  
Author(s):  
Hyo-Jin Lee ◽  
Kyung-Sook Whang
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Gene Sequence ◽  
Morphological Properties ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Content Type ◽  
Link Type ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

The taxonomic position of strain JL-22T, isolated from litter of a bamboo (Sasa borealis) forest, was determined using a polyphasic approach. The organism had phenotypic and morphological properties consistent with it being a member of the genus Streptomyces . Phylogenetic analysis of the 16S rRNA gene sequence showed that strain JL-22T was closely related to Streptomyces prunicolor NRRL B-12281T (99.2 %), Streptomyces galilaeus JCM 4757T (99.0 %) and Streptomyces chartreusis NBRC 12753T (99.0 %). However, the results of DNA–DNA hybridization and physiological and biochemical tests showed that strain JL-22T could be differentiated from its closest phylogenetic relatives both genotypically and phenotypically. Based on phenotypic and genotypic data, strain JL-22T represents a novel species of the genus Streptomyces , for which the name Streptomyces graminifolii sp. nov. is proposed. The type strain is JL-22T ( = KACC 17180T = NBRC 109806T).

scholarly journals Labrys neptuniae sp. nov., isolated from root nodules of the aquatic legume Neptunia oleracea

2007 ◽  
Vol 57 (3) ◽  
pp. 577-581 ◽  
Author(s):  
Yi-Ju Chou ◽  
Geoffrey N. Elliott ◽  
Euan K. James ◽  
Kuan-Yin Lin ◽  
Jui-Hsing Chou ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Root Nodules ◽  
Rrna Gene ◽  
Hybridization Data ◽  
Physiological And Biochemical Characteristics ◽  
Gene Sequence Analysis ◽  
Southern Taiwan ◽  
Physiological And Biochemical ◽  
Fatty Acid Compositions

A bacterium designated strain Liujia-146T was isolated in the Tainan area of southern Taiwan from root nodules of the aquatic legume Neptunia oleracea. 16S rRNA gene sequence analysis indicated that strain Liujia-146T was highly similar to Labrys monachus VKM B-1479T (97.8 %) and Labrys methylaminiphilus JLW10T (95.5 %) and belonged to the order Rhizobiales in the Alphaproteobacteria. On the basis of phylogenetic analysis, DNA–DNA hybridization data, physiological and biochemical characteristics and fatty acid compositions, the organism was shown to belong to the genus Labrys whilst representing a novel species within this genus. We propose to classify strain Liujia-146T (=BCRC 17578T=LMG 23578T) as the type strain of Labrys neptuniae sp. nov.

scholarly journals Aeromonas molluscorum sp. nov., isolated from bivalve molluscs

2004 ◽  
Vol 54 (6) ◽  
pp. 2073-2078 ◽  
Author(s):  
David Miñana-Galbis ◽  
Maribel Farfán ◽  
M. Carme Fusté ◽  
J. Gaspar Lorén
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Fragment Length Polymorphism ◽  
Rrna Gene ◽  
The Novel ◽  
Rrna Gene Sequence ◽  
Bivalve Molluscs ◽  
Gene Sequence Analysis ◽  
Indole Production ◽  
Reference Strains

Five Aeromonas strains (848TT, 93M, 431E, 849T and 869N), which were isolated from bivalve molluscs and were recognized previously by numerical taxonomy as members of an unknown Aeromonas taxon, were subjected to a polyphasic taxonomic study. DNA–DNA hybridization experiments showed that DNA of strain 848TT was <70 % similar (27–45 %) to that of the type/reference strains of the current Aeromonas hybridization groups (HGs), but 93 % similar to that of strain 93M. The DNA G+C content of the five strains ranged from 59·0 to 59·4 mol%. 16S rRNA gene sequence analysis confirmed that the strains belonged to the genus Aeromonas and showed high similarity to Aeromonas encheleia. Amplified fragment length polymorphism fingerprinting clustered the novel strains in a homogeneous group with low genotypic relatedness to other Aeromonas species. Useful phenotypic features for differentiating the five isolates from other Aeromonas species include their negative reactions in tests for indole production, lysine decarboxylase, gas from glucose and starch hydrolysis. From the results of this study, the name Aeromonas molluscorum sp. nov. is proposed for these strains, with the type strain 848TT (=CECT 5864T=LMG 22214T).

scholarly journals Pseudonocardia dioxanivorans sp. nov., a novel actinomycete that grows on 1,4-dioxane

2005 ◽  
Vol 55 (2) ◽  
pp. 593-598 ◽  
Author(s):  
Shaily Mahendra ◽  
Lisa Alvarez-Cohen
Keyword(s):  
Fatty Acids ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Industrial Sludge ◽  
Mycolic Acids ◽  
Physiological And Biochemical Characteristics ◽  
Actinomycete Strain ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

An actinomycete strain (CB1190T) was previously isolated from industrial sludge contaminated with 1,4-dioxane. The cells of this culture are Gram-positive and exhibit branching aerial and vegetative mycelium. Analysis of the 16S rRNA gene sequence indicates that the strain belongs to the genus Pseudonocardia, closely related to Pseudonocardia hydrocarbonoxydans, P. sulfidoxydans and P. halophobica. Physiological and biochemical characteristics of CB1190T are different from those of other known Pseudonocardia species. The novel organism described here is distinguished by its ability to grow on 1,4-dioxane, which is a probable human carcinogen. This culture can also grow on tetrahydrofuran, gasoline aromatics and several other toxic environmental contaminants. Strain CB1190T is capable of fixing dinitrogen. The predominant fatty acids are 16 : 0 iso, 16 : 1 iso cis9 and 17 : 1 iso cis9. The major phospholipid fatty acids are 16 : 0 iso, 16 : 0 10-Me and 17 : 0 10-Me. The peptidoglycan belongs to type A1γ, meso-diaminopimelic acid. The major menaquinone is MK-8 (H4). Mycolic acids are absent. The G+C content is 74 mol%. Based on morphological, physiological, chemotaxonomic and phylogenetic evidence, it is proposed that strain CB1190T (=ATCC 55486T=DSM 44775T) be classified as the type strain of a novel species, Pseudonocardia dioxanivorans sp. nov. Further studies with this organism will provide insights into metabolic pathways, responsible enzymes, kinetics and the fate of 1,4-dioxane in the environment.

scholarly journals Camelimonas fluminis sp. nov., a cyhalothrin-degrading bacterium isolated from river water

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3109-3114 ◽  
Author(s):  
Long Zhang ◽  
Man Song ◽  
Qin Cao ◽  
Shang Wu ◽  
Yan Zhao ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Hybridization Data ◽  
Degrading Bacterium ◽  
Total Dna ◽  
Hanjiang River ◽  
The 16S Rrna Gene

An aerobic, Gram-stain-negative, short rod-shaped, non-spore-forming, cyhalothrin-degrading bacterial strain, XZ2T, was isolated from the surface water of Hanjiang River in Wuhan, China. Strain XZ2T grew optimally at pH 6.0, 30 °C and in the absence of NaCl. The G+C content of the total DNA was 64.1 mol%. The 16S rRNA gene sequence of strain XZ2T showed the highest similarity to that of Camelimonas lactis M 2040T (99.1 %), followed by Camelimonas abortus UK34/07-5T (95.9 %) and Chelatococcus daeguensis K106T (95.3 %). The major cellular fatty acids of strain XZ2T were C19 : 0 cyclo ω8c (63.1 %), C16 : 0 (15.0 %) and C18 : 1ω7c/C18 : 1ω6c (summed feature 8; 8.9 %). C18 : 0 3-OH was also detected as the major hydroxylated fatty acid. The respiratory quinone was ubiquinone Q-10. The polar lipid profile included the major compounds phosphatidylcholine and diphosphatidylglycerol, and moderate amounts of phosphatidylethanolamine, phosphatidylglycerol and two unidentified aminolipids. The predominant compound in the polyamine pattern was spermidine. These chemotaxonomic data supported the affiliation of strain XZ2T to the genus Camelimonas. The DNA–DNA hybridization value between strain XZ2T and Camelimonas lactis M 2040T was 43.5 ± 0.6 %. DNA–DNA hybridization data as well as biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiations between strain XZ2T and Camelimonas lactis M 2040T. Therefore, strain XZ2T represents a novel species of the genus Camelimonas, for which the name Camelimonas fluminis sp. nov. is proposed. The type strain is XZ2T ( = KCTC 42282T = ACCC 19738T).

scholarly journals Marmoricola scoriae sp. nov., isolated from volcanic ash

2010 ◽  
Vol 60 (9) ◽  
pp. 2135-2139 ◽  
Author(s):  
Dong Wan Lee ◽  
Soon Dong Lee
Keyword(s):  
Dna Hybridization ◽  
Volcanic Ash ◽  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Hybridization Data ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Diamino Acid ◽  
Unknown Phospholipid

A novel Gram-stain-positive, coccoid actinobacterium, designated strain Sco-D01T, was isolated from volcanic ash collected from Oreum (a parasitic volcanic cone) on Jeju Island, Republic of Korea. Cells were aerobic, oxidase-negative and catalase-positive. Colonies were vivid yellow, circular, smooth and convex. The diagnostic diamino acid in the cell wall was ll-diaminopimelic acid. The predominant menaquinone was MK-8(H4). The polar lipids were phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unknown phospholipid. The fatty acid profile was represented by large amounts of saturated, unsaturated, 10-methyl and hydroxyl components. The DNA G+C content of strain Sco-D01T was 72.0 mol%. 16S rRNA gene sequence analysis revealed that strain Sco-D01T belonged to the family Nocardioidaceae and formed a distinct sublineage within the radiation of the genus Marmoricola. The level of DNA–DNA relatedness between strain Sco-D01T and its closest phylogenetic relative, Marmoricola aurantiacus DSM 12652T, was 30.2 % (35.4 % in duplicate measurements). On the basis of phenotypic and DNA–DNA hybridization data, strain Sco-D01T is considered to represent a novel species of the genus Marmoricola, for which the name Marmoricola scoriae sp. nov. is proposed. The type strain is Sco-D01T (=KCTC 19597T=DSM 22127T).

Bacillus alkalisediminis sp. nov., an alkaliphilic and moderately halophilic bacterium isolated from sediment of extremely shallow soda ponds

2011 ◽  
Vol 61 (8) ◽  
pp. 1880-1886 ◽  
Author(s):  
Andrea K. Borsodi ◽  
Beatrix Pollák ◽  
Zsuzsa Kéki ◽  
Anna Rusznyák ◽  
Attila L. Kovács ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Optimal Growth ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Separate Species ◽  
Moderately Halophilic Bacterium ◽  
Type Strains

Alkaliphilic strains characterized by optimal growth at pH 9.0 and 5 % (w/v) NaCl designated K1-25T and H3-93 were isolated from extremely shallow soda ponds located in Hungary. Cells of both strains were Gram-stain-positive, non-motile, straight rods and formed central, ellipsoidal endospores with swollen sporangia. The isolates were aerobic, catalase-positive, oxidase-negative and contained a peptidoglycan of type A1γ based on meso-diaminopimelic acid. In both strains, menaquinone-7 (MK-7) was the predominant isoprenoid quinone and the major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The DNA G+C contents of strains K1-25T and H3-93 were 39.0 and 36.3 mol%, respectively. 16S rRNA gene sequence-based phylogenetic analysis revealed 99.2 % similarity between strains K1-25T and H3-93 and the novel isolates had the highest similarities to Bacillus akibai 1139T (97.8 and 98.3 %, respectively), Bacillus wakoensis N-1T (97.0 and 97.4 %), Bacillus okhensis Kh10-101T (97.1 and 97.4 %) and Bacillus krulwichiae AM31DT (96.9 and 97.1 %). DNA–DNA hybridization between our strains and the type strains of closely related Bacillus species was lower than 70 %. Although DNA–DNA hybridization between strains K1-25T and H3-93 was 27 %, the phenotypic and chemotaxonomic data did not support the differentiation of these two strains into separate species. Therefore, they represent genomovars of a novel species, for which the name Bacillus alkalisediminis sp. nov. is proposed. The type strain is K1-25T ( = DSM 21670T  = NCAIM B02301T).

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