scholarly journals Serotype-specific invasive disease potential of Streptococcus pneumoniae in Czech children

2010 ◽  
Vol 59 (9) ◽  
pp. 1079-1083 ◽  
Author(s):  
Helena Zemlickova ◽  
Vladislav Jakubu ◽  
Pavla Urbaskova ◽  
Jitka Motlova ◽  
Martin Musilek ◽  
...  
Keyword(s):  
Czech Republic ◽  
Streptococcus Pneumoniae ◽  
Multilocus Sequence Typing ◽  
Invasive Disease ◽  
Odds Ratios ◽  
The Czech Republic

To estimate the invasive disease potential of Streptococcus pneumoniae serotypes, invasive isolates (n=138) were compared with nasopharyngeal isolates (n=153) from children under 6 years of age in the Czech Republic. Odds ratios (ORs) based on a comparison of the distribution of S. pneumoniae serotypes amongst invasive and carriage isolates were calculated for individual serotypes and 172 strains were characterized using multilocus sequence typing. The ORs of serotypes 9V and 14 were significantly greater than 1, suggesting an association with invasive disease, while serotypes 6A and 23F were significantly associated with carriage (ORs less than 1). A single predominant clone with high invasive disease potential was found in each of the 9V, 7F, 14 and 1 serotypes while carriage-associated serotypes were highly heterogeneous.

scholarly journals Epidemiological relationships among penicillin non-susceptible Streptococcus pneumoniae strains recovered in the Czech Republic

2006 ◽  
Vol 55 (4) ◽  
pp. 437-442 ◽  
Author(s):  
Helena Žemličková ◽  
Oto Melter ◽  
Pavla Urbášková
Keyword(s):  
Czech Republic ◽  
Streptococcus Pneumoniae ◽  
Multilocus Sequence Typing ◽  
Sequence Type ◽  
Penicillin Resistance ◽  
The Czech Republic ◽  
Nationwide Study ◽  
The Past ◽  
Clone Sequence ◽  
Resistance To Penicillin

Since 1986, penicillin non-susceptible pneumococci (PNSP) have been found in the Czech Republic. As documented by a nationwide study, the proportion of invasive strains with reduced susceptibility to penicillin has fluctuated around 5 % in the past decade. Although the level of resistance to penicillin remains stable, the contribution of different capsular serotypes among the PNSP population varies. Whereas serotype 19A was predominantly associated with penicillin resistance until 1997, serotype 9V became most common among PNSP strains in 1998. In a collection of PNSP strains (n=225) isolated from 2000 to 2002, the most frequent serotype was 9V (n=91, 40·4 %), followed by 19F (n=30, 13·3 %) and 14 (n=25, 11·5 %). PFGE and multilocus sequence typing were used to characterize a set of PNSP of the currently predominant serotypes 9V (n=42), 14 (n=15) and 19F (n=14). The Spain9V-3 clone [sequence type (ST) 156] was responsible for a large proportion (100 % of serotype 9V strains, n=42; 93·3 % of serotype 14 strains, n=14) of the analysed strains. A representative of the Taiwan19F-14 clone (ST 236) was also recovered in the Czech Republic (a single isolate of serotype 19F). These findings confirm the spread of the major penicillin-resistant clones in the Czech Republic.

scholarly journals Invasiveness of Serotypes and Clones of Streptococcus pneumoniae among Children in Finland

2005 ◽  
Vol 73 (1) ◽  
pp. 431-435 ◽  
Author(s):  
William P. Hanage ◽  
Tarja H. Kaijalainen ◽  
Ritva K. Syrjänen ◽  
Kari Auranen ◽  
Maija Leinonen ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Confidence Interval ◽  
Multilocus Sequence Typing ◽  
Invasive Disease ◽  
Invasive Infection ◽  
Data Set ◽  
Dramatic Effect ◽  
Life Threatening ◽  
The Difference ◽  
Sequence Types

ABSTRACT Streptococcus pneumoniae (the pneumococcus) causes diseases from otitis media to life-threatening invasive infection. The species is extremely antigenically and clonally diverse. We wished to determine odds ratios (ORs) for serotypes and clones of S. pneumoniae that cause invasive disease in Finland. A total of 224 isolates of S. pneumoniae from cases of invasive disease in children <2 years of age in Finland between 1995 and 1999 were serotyped, and sequence types (STs) were determined by multilocus sequence typing. These STs were compared with a previously published carriage data set. STs from invasive disease were significantly less diverse than those from carriage (invasive disease, 0.038 ± 0.01; carriage, 0.019 ± 0.005). The ORs of serotypes 14, 18C, 19A, and 6B were significantly greater than 1, indicating association with invasive disease. The ORs of 6A and 11A were significantly less than 1. The difference between 6A and 6B is significant, which suggests that relatively subtle changes in the capsule may have a dramatic effect upon disease potential. We found that ST 156, the Spain9V-3 clone which mainly expressed serotype 14 in Finland, is strongly associated with invasive disease (OR, 10.1; 95% confidence interval, 1.3 to 79.5). Significant associations with invasive disease were also detected for STs 482, 191, 124, and 138, and associations with carriage were detected for STs 485 and 62. These results demonstrate the invasive phenotype of the serotype 14 variant of the Spain9V-3 clone and differences between members of the same serogroup in invasive disease potential.

scholarly journals Surveillance of invasive meningococcal disease in the Czech Republic

2004 ◽  
Vol 9 (11) ◽  
pp. 11-12 ◽  
Author(s):  
P Kriz
Keyword(s):  
Czech Republic ◽  
Neisseria Meningitidis ◽  
Meningococcal Disease ◽  
Western Europe ◽  
Vaccination Strategy ◽  
Invasive Disease ◽  
Invasive Meningococcal Disease ◽  
Mortality Data ◽  
Meningococcal Infections ◽  
The Czech Republic

Routine notification of invasive meningococcal disease has a long tradition in the Czech Republic: mortality data are available from 1921 and morbidity data from 1943. The collection of Neisseria meningitidis strains kept in the NRL for Meningococcal Infections in Prague dates from 1970 onwards, and represents more than 3500 strains isolated from invasive disease and their contacts, from healthy carriers and from respiratory infection. Analysis of these strains showed that the Czech meningococcal population is different from that seen in western Europe. In 1993, the incidence serogroup C meningococcal disease increased and was associated with the emergence of the hypervirulent complex Neisseria meningitidis C, ST-11, ET-15/37, and caused an increase in the incidence of invasive meningococcal disease which peaked in 1995 (2.2/100 000). A vaccination strategy targeting the part of the population at highest risk of invasive meningococcal disease was adopted in the country.

scholarly journals Carried Meningococci in the Czech Republic: a Diverse Recombining Population

2000 ◽  
Vol 38 (12) ◽  
pp. 4492-4498 ◽  
Author(s):  
K. A. Jolley ◽  
J. Kalmusova ◽  
E. J. Feil ◽  
S. Gupta ◽  
M. Musilek ◽  
...  
Keyword(s):  
Czech Republic ◽  
Pathogenic Bacteria ◽  
Bacterial Population ◽  
Genetic Relationships ◽  
Invasive Disease ◽  
Sampling Strategies ◽  
The Czech Republic ◽  
Gram Negative ◽  
Nucleotide Sequence Diversity ◽  
Sequence Types

Population and evolutionary analyses of pathogenic bacteria are frequently hindered by sampling strategies that concentrate on isolates from patients with invasive disease. This is especially so for the gram-negative diplococcus Neisseria meningitidis, a cause of septicemia and meningitis worldwide. Meningococcal isolate collections almost exclusively comprise organisms originating from patients with invasive meningococcal disease, although this bacterium is a commensal inhabitant of the human nasopharynx and very rarely causes pathological effects. In the present study, molecular biology-based techniques were used to establish the genetic relationships of 156 meningococci isolated from healthy young adults in the Czech Republic during 1993. None of the individuals sampled had known links to patients with invasive disease. Multilocus sequence typing (MLST) showed that the bacterial population was highly diverse, comprising 71 different sequence types (STs) which were assigned to 34 distinct complexes or lineages. Three previously identified hyperinvasive lineages were present: 26 isolates (17%) belonged to the ST-41 complex (lineage 3); 4 (2.6%) belonged to the ST-11 (electrophoretic type [ET-37]) complex, and 1 (0.6%) belonged to the ST-32 (ET-5) complex. The data were consistent with the view that most nucleotide sequence diversity resulted from the reassortment of alleles by horizontal genetic exchange.

O480 Characterisation of Neisseria meningitidis B causing invasive disease in the Czech Republic

2007 ◽  
Vol 29 ◽  
pp. S102-S103
Author(s):  
P. Kriz ◽  
J. Kalmusova ◽  
M. Musilek ◽  
J. Felsberg ◽  
R. Haugvicova ◽  
...  
Keyword(s):  
Czech Republic ◽  
Neisseria Meningitidis ◽  
Invasive Disease ◽  
The Czech Republic

scholarly journals Streptococcus pneumoniae serotype 3 genotypes in invasive isolates from Colombia

Biomédica ◽  
2021 ◽  
Vol 41 (2) ◽  
pp. 338-346
Author(s):  
Zonia Katerin Alarcón ◽  
Carolina Duarte ◽  
Olga Sanabria ◽  
Jaime Moreno
Keyword(s):  
Streptococcus Pneumoniae ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Genetic Similarity ◽  
Invasive Disease ◽  
Pulsed Field Gel Electrophoresis ◽  
Surveillance Program ◽  
National Surveillance ◽  
Capsular Type ◽  
Type 3

Introduction: Streptococcus pneumoniae serotype 3 is an important cause of pneumonia, bacteremia, and meningitis.Objective: To establish the circulating genotypes of S. pneumoniae serotype 3 isolates recovered from the invasive disease between 1994 to 2015 in Colombia.Materials and methods: Of the 365 S. pneumoniae serotype 3 isolates recovered through the laboratory national surveillance program, 117 isolates were analyzed. Pulsed-field gel electrophoresis was used for genotyping, and multilocus sequence typing was determined in representative isolates.Results: The frequency of this serotype increased from 2.7% between 1994 and 1998 to 9.1% between 2011 and 2015 (p=0.000); 91.7% of the isolates showed a genetic similarity greater than 77% and were related to the Netherlands3-31(PMEN31) clone CC180. Several subtypes were identified, two of which showed antimicrobial resistance.Conclusion: In Colombia, the pneumococcal population of the capsular type 3 shows a continuous and homogeneous circulation relating to the clonal group ST-180.

scholarly journals Corrigendum: Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era

2018 ◽  
Vol 67 (8) ◽  
pp. 1202-1202 ◽  
Author(s):  
Helena Žemličková ◽  
Lucia Mališová ◽  
Petra Španělová ◽  
Vladislav Jakubů ◽  
Jana Kozáková ◽  
...  
Keyword(s):  
Czech Republic ◽  
Streptococcus Pneumoniae ◽  
Molecular Characterization ◽  
The Czech Republic
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