scholarly journals Use of a multiplexed immunoassay for detection of serotype-specific Streptococcus pneumoniae antigen in pleural fluid and cerebrospinal fluid specimens

2011 ◽  
Vol 60 (12) ◽  
pp. 1879-1881 ◽  
Author(s):  
Carmen L. Sheppard ◽  
Malcolm Guiver ◽  
John Hartley ◽  
Timothy G. Harrison ◽  
Robert C. George
Respiration ◽  
2007 ◽  
Vol 75 (4) ◽  
pp. 437-442 ◽  
Author(s):  
G. Eda Utine ◽  
Ahmet Pinar ◽  
Uğur Özçelik ◽  
Burçin Şener ◽  
Ebru Yalçin ◽  
...  

2018 ◽  
Vol 36 (7) ◽  
pp. 428-430 ◽  
Author(s):  
Juan Carlos Sanz ◽  
Esther Ríos ◽  
Iciar Rodríguez-Avial ◽  
Belén Ramos ◽  
Mercedes Marín ◽  
...  

1997 ◽  
Vol 41 (9) ◽  
pp. 2050-2052 ◽  
Author(s):  
C Doit ◽  
J Barre ◽  
R Cohen ◽  
S Bonacorsi ◽  
A Bourrillon ◽  
...  

Cerebrospinal fluid (CSF) was taken from 19 children with bacterial meningitis treated with cefotaxime (300 mg/kg of body weight/day) and vancomycin (60 mg/kg/day). Median levels of drugs in CSF were smaller than expected, as follows: 4.4 microg/ml for cefotaxime, 3.2 microg/ml for desacetylcefotaxime, and 1.7 microg/ml for vancomycin. The median CSF bactericidal titer against an intermediately cefotaxime-resistant pneumococcus was 1:4. Our data suggest at least an additive interaction between the drugs used in this study.


2020 ◽  
Vol 11 (4) ◽  
pp. 102-105
Author(s):  
Ummer Karadan ◽  
Robin George Manappallil ◽  
Manoj Bhaskaran ◽  
Anoop Chandran ◽  
Ivin Panakkel Zacharia

Parainfectious vasculitis is a rare life threatening complication of Streptococcus pneumoniae (S.pneumoniae) meningitis. There is a 19% risk of developing meningitis in the patients with cerebrospinal fluid (CSF) rhinorrhoea. The patient being reported developed CSF rhinorrhoea due to traumatic fracture of cribriform plate, followed by pneumococcal meningitis and parainfectious vasculitis resulting in cerebellar infarction.


2019 ◽  
Vol 57 (10) ◽  
Author(s):  
Sun Jin Kim ◽  
Yoo Jung Jeong ◽  
Jong Hun Kim ◽  
Young Kyung Yoon ◽  
Jang Wook Sohn ◽  
...  

ABSTRACT The multiplexed immunoassay (MIA) is an automated, monoclonal antibody-based serotyping assay that uses culture lysates of Streptococcus pneumoniae. This study describes the development and validation of applying MIA directly to sputum samples for the serotype-specific detection of S. pneumoniae. Sputum optimization involved liquefaction and fractionation. The subjects included 173 adult patients from whom both pneumococcal isolates cultured from sputum samples and the corresponding sputum samples were available at the Korea University Hospital from March 2012 to June 2015. Pneumococcal lysates and the sputum fraction were separately evaluated by MIA with a set A reaction to identify 27 serotypes (24 vaccine serotypes and serotypes 6C, 6D, and 11E). MIA results were validated by multiplex PCR (mPCR). Among the 173 patients analyzed, the pneumococcal isolate MIA detected a single set A serotype in 104 patients, and the corresponding sputum MIA showed concordant results with additional multiple serotypes in 21 patients. For the remaining 69 patients whose pneumococcal isolates were not determined to be set A serotypes by the pneumococcal isolate MIA, the corresponding sputum MIA identified additional set A serotypes (single serotypes, n = 17; multiple serotypes, n = 4). Serotypes 3 and 11A/D/F were the most commonly detected serotypes in both the pneumococcal isolate and sputum MIA analyses. However, serotype 8 was the most prevalent serotype detected only by the sputum MIA. The results of mPCR, performed for validation, showed a high concordance with the results of the sputum MIA. In conclusion, MIA using sputum samples enables the accurate, rapid, direct, and serotype-specific detection of S. pneumoniae, which may improve postvaccination serotype surveillance.


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