Development of a sandwich dot–enzyme linked immunosorbent assay for Streptococcus pneumoniae antigen detection in cerebrospinal fluid

2004 ◽  
Vol 27 (3) ◽  
pp. 217-223 ◽  
Author(s):  
G.P Rai ◽  
K Zachariah ◽  
R Sharma ◽  
S Phadake ◽  
K.M Belapurkar
Keyword(s):  
Cerebrospinal Fluid ◽  
Streptococcus Pneumoniae ◽  
Immunosorbent Assay ◽  
Antigen Detection ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Identification of a Secreted Cholesterol-Dependent Cytolysin (Mitilysin) from Streptococcus mitis

2006 ◽  
Vol 189 (2) ◽  
pp. 627-632 ◽  
Author(s):  
Johanna Jefferies ◽  
Leena Nieminen ◽  
Lea-Ann Kirkham ◽  
Calum Johnston ◽  
Andrew Smith ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Streptococcus Pneumoniae ◽  
Amino Acid ◽  
Immunosorbent Assay ◽  
Pneumococcal Disease ◽  
Neutralization Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Amino Acid Substitutions ◽  
Streptococcus Mitis ◽  
Hemolytic Toxin

ABSTRACT We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mitilysin extracellularly. Certain alleles of mitilysin are not recognized by a monoclonal antibody raised to the related toxin pneumolysin. Based on enzyme-linked immunosorbent assay and neutralization assay results, one isolate of S. mitis may produce a further hemolytic toxin in addition to mitilysin. As genetic exchange is known to occur between S. mitis and Streptococcus pneumoniae, this finding may have implications for the development of vaccines or therapies for pneumococcal disease that are based on pneumolysin.

scholarly journals Detection of Antigenemia by Enzyme-Linked Immunosorbent Assay in Horses with Experimental Ehrlichia Risticii Infection

1993 ◽  
Vol 5 (1) ◽  
pp. 33-36 ◽  
Author(s):  
Richard E. Corstvet ◽  
Stephen D. Gaunt ◽  
Phillip A. Karns ◽  
David Burgermeister ◽  
Jere W. McBride ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Detection Method ◽  
Serum Antibody ◽  
Clinical Signs ◽  
Clinical Findings ◽  
Antigen Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Horse ◽  
Ehrlichia Risticii

Four horses were inoculated with Ehrlichia risticii contained in either infected murine P388 D1 cells or heparinized blood from an infected horse. All 4 horses produced serum antibody, plasma antigen, and clinical signs of the disease. An enzyme-linked immunosorbent assay was used to detect antibody in the serum and was also used in conjunction with an anti- E. risticii monoclonal antibody to detect antigenemia. These laboratory and clinical findings were correlated to determine the efficiency of the antigen detection method for discerning E. risticii infection.

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