scholarly journals Trehalose metabolism is important for heat stress tolerance and spore germination of Botrytis cinerea

Microbiology ◽  
2006 ◽  
Vol 152 (9) ◽  
pp. 2625-2634 ◽  
Author(s):  
Gunther Doehlemann ◽  
Patrick Berndt ◽  
Matthias Hahn
Keyword(s):  
Heat Stress ◽  
Botrytis Cinerea ◽  
Grey Mould ◽  
Wild Type ◽  
Hydrophobic Surfaces ◽  
Mould Fungus ◽  
Germination Process ◽  
Plant Pathogenicity ◽  
As Stress ◽  
Stress Protectant

To analyse the role of trehalose as stress protectant and carbon storage compound in the grey mould fungus Botrytis cinerea, mutants defective in trehalose-6-phosphate synthase (TPS1) and neutral trehalase (TRE1) were constructed. The Δtps1 mutant was unable to synthesize trehalose, whereas the Δtre1 mutant showed elevated trehalose levels compared to the wild-type and was unable to mobilize trehalose during conidial germination. Both mutants showed normal vegetative growth and were not affected in plant pathogenicity. Growth of the Δtps1 mutant was more heat sensitive compared to the wild-type. Similarly, Δtps1 conidia showed a shorter survival under heat stress, and their viability at moderate temperatures was strongly reduced. In germinating wild-type conidia, rapid trehalose degradation occurred only when germination was induced in the presence of nutrients. In contrast, little trehalose breakdown was observed during germination on hydrophobic surfaces in water. Here, addition of cAMP to conidia induced trehalose mobilization and accelerated the germination process, probably by activation of TRE1. In accordance with these data, both mutants showed germination defects only in the presence of sugars but not on hydrophobic surfaces in the absence of nutrients. The data indicate that in B. cinerea trehalose serves as a stress protectant, and also as a significant but not essential carbon source for germination when external nutrients are low. In addition, evidence was obtained that trehalose 6-phosphate plays a role as a regulator of glycolysis during germination.

scholarly journals A novel Botrytis cinerea‐ specific gene BcHBF1 enhances virulence of the grey mould fungus via promoting host penetration and invasive hyphal development

2019 ◽  
Vol 20 (5) ◽  
pp. 731-747 ◽  
Author(s):  
Yue Liu ◽  
Jiane‐Kang Liu ◽  
Gui‐Hua Li ◽  
Ming‐Zhe Zhang ◽  
Ying‐Ying Zhang ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Grey Mould ◽  
Specific Gene ◽  
Mould Fungus ◽  
Hyphal Development ◽  
Host Penetration

scholarly journals Fungicide-Driven Evolution and Molecular Basis of Multidrug Resistance in Field Populations of the Grey Mould Fungus Botrytis cinerea

2009 ◽  
Vol 5 (12) ◽  
pp. e1000696 ◽  
Author(s):  
Matthias Kretschmer ◽  
Michaela Leroch ◽  
Andreas Mosbach ◽  
Anne-Sophie Walker ◽  
Sabine Fillinger ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Botrytis Cinerea ◽  
Molecular Basis ◽  
Grey Mould ◽  
Mould Fungus

scholarly journals RLP23 is required for Arabidopsis immunity against the grey mould pathogen Botrytis cinerea

2020 ◽  
Author(s):  
Erika Ono ◽  
Kazuyuki Mise ◽  
Yoshitaka Takano
Keyword(s):  
Botrytis Cinerea ◽  
Plant Immunity ◽  
Late Phase ◽  
Grey Mould ◽  
Early Infection ◽  
Alternaria Brassicicola ◽  
Dependent Manner ◽  
Wild Type ◽  
Actual Contribution ◽  
Molecular Patterns

AbstractNecrosis- and ethylene-inducing-like proteins (NLPs) are secreted by fungi, oomycetes and bacteria. Conserved nlp peptides derived from NLPs are recognized as pathogen-associated molecular patterns (PAMPs), leading to PAMP-triggered immune responses. RLP23 is the receptor of the nlp peptides in Arabidopsis thaliana; however, its actual contribution to plant immunity is unclear. Here, we report that RLP23 is required for Arabidopsis immunity against the necrotrophic fungal pathogen Botrytis cinerea. Arabidopsis rlp23 mutants exhibited enhanced susceptibility to B. cinerea compared with the wild-type plants. Notably, microscopic observation of the B. cinerea infection behaviour indicated the involvement of RLP23 in pre-invasive resistance to the pathogen. B. cinerea carried two NLP genes, BcNEP1 and BcNEP2; BcNEP1 was expressed preferentially before/during invasion into Arabidopsis, whereas BcNEP2 was expressed at the late phase of infection. Importantly, the nlp peptides derived from both BcNEP1 and BcNEP2 induced the production of reactive oxygen species in an RLP23-dependent manner. In contrast, other necrotrophic fungus Alternaria brassicicola did not express the NLP gene in the early infection phase and exhibited no enhanced virulence in the rlp23 mutants. Collectively, these results strongly suggest that RLP23 contributes to Arabidopsis pre-invasive resistance to B. cinerea via NLP recognition at the early infection phase.

Apoptosis-like programmed cell death in the grey mould fungus Botrytis cinerea: genes and their role in pathogenicity

2011 ◽  
Vol 39 (5) ◽  
pp. 1493-1498 ◽  
Author(s):  
Neta Shlezinger ◽  
Adi Doron ◽  
Amir Sharon
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Botrytis Cinerea ◽  
Fungal Pathogens ◽  
Grey Mould ◽  
Fungal Species ◽  
Fungal Virulence ◽  
Mould Fungus ◽  
Apoptosis Protein ◽  
Result Show

A considerable number of fungal homologues of human apoptotic genes have been identified in recent years. Nevertheless, we are far from being able to connect the different pieces and construct a primary structure of the fungal apoptotic regulatory network. To get a better picture of the available fungal components, we generated an automatic search protocol that is based on protein sequences together with a domain-centred approach. We used this protocol to search all the available fungal databases for domains and homologues of human apoptotic proteins. Among all known apoptotic domains, only the BIR [baculovirus IAP (inhibitor of apoptosis protein) repeat] domain was found in fungi. A single protein with one or two BIR domains is present in most (but not all) fungal species. We isolated the BIR-containing protein from the grey mould fungus Botrytis cinerea and determined its role in apoptosis and pathogenicity. We also isolated and analysed BcNMA, a homologue of the yeast NMA11 gene. Partial knockout or overexpression strains of BcBIR1 confirmed that BcBir1 is anti-apoptotic and this activity was assigned to the N′-terminal part of the protein. Plant infection assays showed that the fungus undergoes massive PCD (programmed cell death) during early stages of infection. Further studies showed that fungal virulence was fully correlated with the ability of the fungus to cope with plant-induced PCD. Together, our result show that BcBir1 is a major regulator of PCD in B. cinerea and that proper regulation of the host-induced PCD is essential for pathogenesis in this and other similar fungal pathogens.

Management of Botrytis grey mould caused by Botrytis cinerea in lentil using boscalid fungicide

2021 ◽  
pp. 1-10
Author(s):  
Mark E. Kuchuran ◽  
Cheryl Armstrong-Cho ◽  
Sabine Banniza
Keyword(s):  
Botrytis Cinerea ◽  
Grey Mould

An ER–Golgi Tethering Factor SLOH4/MIP3 Is Involved in Long-term Heat Tolerance of Arabidopsis

2020 ◽  
Author(s):  
Kazuho Isono ◽  
Ryo Tsukimoto ◽  
Satoshi Iuchi ◽  
Akihisa Shinozawa ◽  
Izumi Yotsui ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Tolerance ◽  
Secretory Proteins ◽  
Wild Type ◽  
Additional Function ◽  
Transcript Levels ◽  
Unfolded Protein ◽  
In The Wild ◽  
Protein Response

Abstract Plants are often exposed not only to short-term (S-) heat stress but also to diurnal long-term (L-) heat stress over several consecutive days. To reveal the mechanisms underlying L-heat stress tolerance, we here used a forward genetic screening for sensitive to long-term heat (sloh) mutants and isolated sloh4. The mutant was hypersensitive to L- but not S-heat stress. The causal gene of sloh4 was identical to MIP3 encoding a member of the MAIGO2 (MAG2) tethering complex, which is composed of the MAG2, MIP1, MIP2, and MIP3 subunits and is localized at the endoplasmic reticulum (ER) membrane. Although sloh4/mip3 was hypersensitive to L-heat stress, the sensitivity of the mag2-3 and mip1–1 mutants was similar to that of the wild type. Under L-heat stress, the ER stress and the following unfolded protein response (UPR) were more pronounced in sloh4 than in the wild type. Transcript levels of bZIP60-regulated UPR genes were strongly increased in sloh4 under L-heat stress. Two processes known to be mediated by INOSITOL REQUIRING ENZYME1 (IRE1)—accumulation of the spliced bZIP60 transcript and a decrease in the transcript levels of PR4 and PRX34, encoding secretory proteins—were observed in sloh4 in response to L-heat stress. These findings suggest that misfolded proteins generated in sloh4 under L-heat stress may be recognized by IRE1 but not bZIP28, resulting in initiation of the UPR via activated bZIP60. Therefore, it would be possible that only MIP3 in MAG2 complex has an additional function in L-heat tolerance, which is not related to the ER–Golgi vesicle tethering.

Biocontrol ability and action mechanism of Bacillus halotolerans against Botrytis cinerea causing grey mould in postharvest strawberry fruit

2021 ◽  
Vol 174 ◽  
pp. 111456
Author(s):  
Fang Wang ◽  
Jie Xiao ◽  
Yunzeng Zhang ◽  
Raoyong Li ◽  
Li Liu ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Grey Mould ◽  
Strawberry Fruit ◽  
Action Mechanism

scholarly journals Investigation of Botrytis Cinerea Risk Forecasting Model of Strawberry in Lithuania

2013 ◽  
Vol 67 (2) ◽  
pp. 195-198 ◽  
Author(s):  
Neringa Rasiukevičiūtė ◽  
Alma Valiuškaitė ◽  
Elena Survilienė-Radzevičė ◽  
Skaidrė Supronienė
Keyword(s):  
Disease Management ◽  
Botrytis Cinerea ◽  
Air Temperature ◽  
Grey Mould ◽  
Management Systems ◽  
Forecasting Model ◽  
Leaf Wetness ◽  
Risk Of Infection ◽  
Risk Forecasting ◽  
Risk Probability

Grey mould, caused by Botrytis cinerea Pers.:Fr. is one of the most important strawberry diseases in Lithuania, like in other countries, where strawberries are grown. The efficiency of different disease management systems were analyzed at the Institute of Horticulture in 2010-2011. The B. cinerea risk probability at various regions of Lithuania was analyzed according to iMETOS ®sm grey mould risk forecasting model. Strawberry grey mould risk forecasting model indicates the risk of infection periods on the basis of the interaction between air temperature and leaf wetness duration. The model calculates how favourable is the period for the risk of infection. In periods where the risk is consistent (more than three days), higher than 60 points, a spray against grey mould should be applied. iMETOS®sm grey mould risk forecasting model gives the opportunity to optimize the usage of fungicides and reduce the number of applications and allows more efficient, ecologically and economically accepted control of strawberries grey mould.

Sign in / Sign up

Export Citation Format

Share Document