Circular genomes related to anelloviruses identified in human and animal samples by using a combined rolling-circle amplification/sequence-independent single primer amplification approach

A combined rolling-circle amplification (RCA) and sequence-independent single primer amplification (SISPA) approach was applied to four samples of human plasma and one sample of saliva from a cat. This approach permitted the characterization of nine anelloviruses. Most of them were identified as highly divergent strains that were classified into species of the genus Anellovirus. The smallest anellovirus described so far in humans was characterized (2PoSMA, 2002 nt; ‘small anellovirus’ species). Two highly divergent sequences belonging to the species Torque Teno Mini Virus (LIL-y1, 2887 nt; LIL-y2, 2871 nt), which clustered into a new phylogenetic branch, were also identified in human plasma samples. Finally, two genomes that are separated by a genetic divergence of 46 % were characterized in the cat's saliva, one of these creating a distinct phylogenetic branch (PRA1, 2019 nt). These results highlight the potential of RCA–SISPA for detecting circular (or circularized) genomes.

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Mass spectrometric characterization of limited proteolysis activity in human plasma samples under mild acidic conditions

Methods ◽

10.1016/j.ymeth.2015.02.013 ◽

2015 ◽

Vol 89 ◽

pp. 30-37 ◽

Cited By ~ 4

Author(s):

Jingzhi Yang ◽

Claudia Röwer ◽

Cornelia Koy ◽

Manuela Ruß ◽

Christopher P. Rüger ◽

...

Keyword(s):

Human Plasma ◽

Limited Proteolysis ◽

Mass Spectrometric ◽

Plasma Samples ◽

Human Plasma Samples ◽

Acidic Conditions

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Development and validation of a selective SPR aptasensor for the detection of anticancer drug irinotecan in human plasma samples

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-020-03087-5 ◽

2021 ◽

Author(s):

Adelina Puscasu ◽

Martina Zanchetta ◽

Bianca Posocco ◽

David Bunka ◽

Stefano Tartaggia ◽

...

Keyword(s):

Human Plasma ◽

Anticancer Drug ◽

Plasma Samples ◽

Human Plasma Samples ◽

Development And Validation

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Protocol to analyze circulating small non-coding RNAs by high-throughput RNA sequencing from human plasma samples

STAR Protocols ◽

10.1016/j.xpro.2021.100606 ◽

2021 ◽

Vol 2 (3) ◽

pp. 100606

Author(s):

Giuseppina E. Grieco ◽

Guido Sebastiani ◽

Daniela Fignani ◽

Noemi Brusco ◽

Laura Nigi ◽

...

Keyword(s):

Human Plasma ◽

Rna Sequencing ◽

High Throughput ◽

Plasma Samples ◽

Human Plasma Samples ◽

Non Coding Rnas

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Optimizationvia experimental design of an SPE-HPLC-UV-fluorescence method for the determination of valsartan and its metabolite in human plasma samples

Journal of Separation Science ◽

10.1002/jssc.200600093 ◽

2006 ◽

Vol 29 (15) ◽

pp. 2265-2283 ◽

Cited By ~ 24

Author(s):

Gorka Iriarte ◽

Nerea Ferreirós ◽

Izaskun Ibarrondo ◽

Rosa Maria Alonso ◽

Miren Itxaso Maguregi ◽

...

Keyword(s):

Experimental Design ◽

Human Plasma ◽

Fluorescence Method ◽

Plasma Samples ◽

Uv Fluorescence ◽

Human Plasma Samples

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Comparison of 4-plex to 8-plex iTRAQ Quantitative Measurements of Proteins in Human Plasma Samples

Journal of Proteome Research ◽

10.1021/pr300414z ◽

2012 ◽

Vol 11 (7) ◽

pp. 3774-3781 ◽

Cited By ~ 53

Author(s):

Gwenael Pottiez ◽

Jayme Wiederin ◽

Howard S. Fox ◽

Pawel Ciborowski

Keyword(s):

Human Plasma ◽

Plasma Samples ◽

Quantitative Measurements ◽

Human Plasma Samples

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Characterization of Two Novel Polyomaviruses of Birds by Using Multiply Primed Rolling-Circle Amplification of Their Genomes

Journal of Virology ◽

10.1128/jvi.80.7.3523-3531.2006 ◽

2006 ◽

Vol 80 (7) ◽

pp. 3523-3531 ◽

Cited By ~ 54

Author(s):

Reimar Johne ◽

Walter Wittig ◽

Daniel Fernández-de-Luco ◽

Ursula Höfle ◽

Hermann Müller

Keyword(s):

Rolling Circle Amplification ◽

Large Tumor ◽

Rolling Circle ◽

Reading Frame ◽

Field Samples ◽

Close Relationship ◽

Avian Polyomavirus ◽

Avian Group ◽

Pyrrhula Pyrrhula

ABSTRACT Polyomaviruses are small nonenveloped particles with a circular double-stranded genome, approximately 5 kbp in size. The mammalian polyomaviruses mainly cause persistent subclinical infections in their natural nonimmunocompromised hosts. In contrast, the polyomaviruses of birds—avian polyomavirus (APV) and goose hemorrhagic polyomavirus (GHPV)—are the primary agents of acute and chronic disease with high mortality rates in young birds. Screening of field samples of diseased birds by consensus PCR revealed the presence of two novel polyomaviruses in the liver of an Eurasian bullfinch (Pyrrhula pyrrhula griseiventris) and in the spleen of a Eurasian jackdaw (Corvus monedula), tentatively designated as finch polyomavirus (FPyV) and crow polyomavirus (CPyV), respectively. The genomes of the viruses were amplified by using multiply primed rolling-circle amplification and cloned. Analysis of the FPyV and CPyV genome sequences revealed a close relationship to APV and GHPV, indicating the existence of a distinct avian group among the polyomaviruses. The main characteristics of this group are (i) involvement in fatal disease, (ii) the existence of an additional open reading frame in the 5′ region of the late mRNAs, and (iii) a different manner of DNA binding of the large tumor antigen compared to that of the mammalian polyomaviruses.

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