scholarly journals Genomic Profiling of a Human Leukemic Monocytic Cell-Line (THP-1) Exposed to Alpha Particle Radiation

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Vinita Chauhan ◽  
Matthew Howland
Keyword(s):  
Gene Expression ◽  
Radiation Effects ◽  
Assessment Tool ◽  
Late Onset ◽  
Dose Range ◽  
Biological Assessment ◽  
Global Changes ◽  
Monocytic Cell ◽  
Particle Radiation ◽  
Monocytic Cell Line

This study examined alpha (α-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed toα-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure. Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure. Among these genes, 6 genes were time and dose responsive and validated further using alternate technology. These transcripts were upregulated and associated with biological processes related to immune function, organelle stability and cell signalling/communication. This panel of genes merits further validation to determine if they are strong candidate biomarkers indicative ofα-particle exposure.

scholarly journals Repression of human cytomegalovirus major immediate early gene expression in a monocytic cell line

1992 ◽  
Vol 73 (2) ◽  
pp. 433-435 ◽  
Author(s):  
J. H. Sinclair ◽  
J. Baillie ◽  
L. A. Bryant ◽  
J. A. Taylor-Wiedeman ◽  
J. G. P. Sissons
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Human Cytomegalovirus ◽  
Immediate Early Gene ◽  
Early Gene ◽  
Immediate Early ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Early Gene Expression

scholarly journals Leishmania major Abrogates Gamma Interferon-Induced Gene Expression in Human Macrophages from a Global Perspective

2007 ◽  
Vol 75 (7) ◽  
pp. 3506-3515 ◽  
Author(s):  
Nisha Dogra ◽  
Corinna Warburton ◽  
W. Robert McMaster
Keyword(s):  
Gene Expression ◽  
Host Cell ◽  
Large Scale ◽  
Leishmania Major ◽  
Gamma Interferon ◽  
Receptor Expression ◽  
Global Perspective ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Ifn Γ

ABSTRACT Infection with Leishmania major triggers several pathways in the host cell that are crucial to initial infection as well as those that are used by Leishmania to enhance its replication and virulence. To identify the molecular events of the host cell in response to Leishmania, the global gene expression of the human monocytic cell line THP-1 either infected with Leishmania major in the presence and absence of gamma interferon (IFN-γ) or in the presence of IFN-γ alone was analyzed using high-density human oligonucleotide microarrays, followed by statistical analysis. The persistence of the parasite despite an extensive response to IFN-γ, added 24 h after infection with L. major, suggests that L. major can survive in an IFN-γ-enriched environment in vitro. Results demonstrate that L. major counteracts the IFN-γ response in macrophages on a large scale. Expression of genes involved in the innate immune response, cell adhesion, proteasomal degradation, Toll-like receptor expression, a variety of signaling molecules, and matrix metalloproteinases was significantly modulated.

The response of a mixed cell population in mouse testes to x-ray and HZE irradiation

1983 ◽  
Vol 41 ◽  
pp. 674-675
Author(s):  
D. E. Philpott ◽  
W. Sapp ◽  
C. Williams ◽  
J. Stevenson ◽  
S. Black ◽  
...  
Keyword(s):  
Radiation Effects ◽  
Dose Range ◽  
Life Cycles ◽  
X Rays ◽  
Mixed Cell ◽  
Particle Radiation ◽  
Thin Sections ◽  
Straight Line ◽  
Mixed Cell Population ◽  
Standard Manner

The testes sperraatogonial population differentiates from a stem cell (As) which, via a series of mitotic divisions, gives rise to spermatocytes. The spermatocytes undergo reductional meiotic divisions and mature into sperm. Short cellular life cycles make the testes an excellent tissue for quantitative studies of radiation effects.Brown B6D2F1 mice, aged 14 weeks, were exposed to Helium particle radiation (227 MeV) in the Berkeley 184 inch synchocyclotron and to x rays at 225 KvP and 15 mA. Doses ranged from 1 to 100 rads. Tissues were fixed in Triple Fix and embedded. Two-micron sections were stained with Toluidine Blue, and ultra-thin sections were stained in the standard manner for electron microscopy. All studies were done on the tissues 72 hrs after irradiation.Our previous work, on cell survival after graded doses of radiation, has shown a steeper slope (more sensitive response) in the low dose range below 20 rads. By convention, the resulting points were plotted by linear regression resulting in a "best fit" straight line.

The effect of an anti-inflammatory pentapeptide produced by Entamoeba histolytica on gene expression in the U-937 monocytic cell line

2008 ◽  
Vol 57 (4) ◽  
pp. 145-150 ◽  
Author(s):  
R. Silva-García ◽  
I. Estrada-García ◽  
R. Ramos-Payán ◽  
A. Torres-Salazar ◽  
M. E. Morales-Martínez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Entamoeba Histolytica ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Anti Inflammatory

Control of human cytomegalovirus gene expression by differential histone modifications during lytic and latent infection of a monocytic cell line

Gene ◽  
2006 ◽  
Vol 384 ◽  
pp. 120-128 ◽  
Author(s):  
Elena Ioudinkova ◽  
Maria Cristina Arcangeletti ◽  
Alla Rynditch ◽  
Flora De Conto ◽  
Federica Motta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Human Cytomegalovirus ◽  
Histone Modifications ◽  
Latent Infection ◽  
Monocytic Cell ◽  
Monocytic Cell Line
Sign in / Sign up

Export Citation Format

Share Document