scholarly journals An Efficient In Vitro Propagation Protocol of Cocoyam [Xanthosoma sagittifolium(L) Schott]

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Anne E. Sama ◽  
Harrison G. Hughes ◽  
Mohamed S. Abbas ◽  
Mohamed A. Shahba
Keyword(s):  
Dimethyl Sulfoxide ◽  
In Vitro Propagation ◽  
Apical Meristem ◽  
Culture Medium ◽  
Naphthaleneacetic Acid ◽  
Liquid Media ◽  
Mineral Salts ◽  
Axillary Shoots ◽  
Xanthosoma Sagittifolium

Sprouted corm sections of “South Dade” white cocoyam were potted and maintained in a greenhouse for 8 weeks. Shoot tips of 3–5 mm comprising the apical meristem with 4–6 leaf primordial, and approximately 0.5 mm of corm tissue at the base. These explants were treated to be used into the culture medium. A modified Gamborg’s B5 mineral salts supplemented with 0.05 μM 1-naphthaleneacetic acid (NAA) were used throughout the study. Thidiazuron (TDZ) solution containing 0.01% dimethyl sulfoxide (DMSO) was used. Erlenmeyer flasks and test tubes were used for growing cultures. The effect of different media substrate, thidiazuron, and the interaction between TDZ and Benzylaminopurine (BAP) on cocoyam culture were tested. Results indicated that cocoyam can be successfully micropropagated in vitro through various procedures. All concentrations tested (5–20 μM BAP and 1–4 μM TDZ) produced more axillary shoots per shoot tip than the control without cytokinins. Greater proliferation rates were obtained through the use of 20 μM BAP and 2 μM TDZ, respectively, 12 weeks from initiation. Shoots produced with BAP were larger and more normal in appearance than those produced with TDZ, which were small, compressed, and stunted. The use of stationary liquid media is recommended for economic reasons.

scholarly journals The influence of the mineral composition of the medium on in vitro propagation of Kohleria amabilis (Planch. et Linden) Fritsch shoots

2012 ◽  
Vol 60 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Danuta Kozak ◽  
Jerzy Hetman ◽  
Małgorzata Witek
Keyword(s):  
Mineral Composition ◽  
Significant Influence ◽  
In Vitro Propagation ◽  
Passage Time ◽  
Salt Composition ◽  
Mineral Salts ◽  
Axillary Shoots ◽  
Half Strength ◽  
Medium Type

The aim of the study was to test the influence of the mineral salt composition of the medium on <i>in vitro</i> multiplication and growth of kohleria shoots. Shoot tips were cultured on Murashige and Skoog (MS) (full- and half strength), Gamborg et al. (B<sub>5</sub>), Nitsch and Nitsch (NN) or Lloyd and McCown (WPM) media supplemented with BA 1 mg·dm<sup>-3</sup>. The influence of passage time on the induction and growth of shoots on the medium containing half strength of MS mineral salts and BA 1 mg·dm<sup>-3</sup>, GA<sub>3</sub> 1 mg·dm<sup>-3</sup> was also studied. The explants used in the experiments were obtained from aseptically grown shoot clusters. A significant influence of the medium type and the time of propagation on the number, length and weight of axillary shoots was observed. The medium containing full or half strength of mineral salts according to MS was the best. The propagation of kohleria shoots should take place at 4-week passages.

scholarly journals Micropropagation of the Endangered and Decorative Specie Dianthus serotinus Waldst. et Kit.

2013 ◽  
Vol 41 (2) ◽  
pp. 370 ◽  
Author(s):  
Marija MARKOVIĆ ◽  
Mihailo GRBIĆ ◽  
Matilda DJUKIĆ
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Shoot Multiplication ◽  
Naphthaleneacetic Acid ◽  
Endangered Plant ◽  
Ms Medium ◽  
Medium Ph ◽  
Half Strength ◽  
Terminal Buds

During past decades, great attention has been paid to propagation of endangered plant taxa in order to preserve biodiversity. The aim of this study was to optimize a protocol for in vitro propagation of the critically endangered and decorative species Dianthus serotinus Waldst. et Kit. The effects of different concentration of MS salt (Murashige and Skoog) of the culture, medium pH and different carbohydrates (sucrose, glucose, and fructose) on shoot multiplication were examined. The best results were obtained on half-strength MS (Murashige and Skoog) medium, whose pH was 5.8, with sucrose supplied at a concentration of 3%, when shoots with 1-2 nodes or shoot tips (with terminal buds only) were used as explants. The shoots were rooted (76.7%) on half-strength MS medium containing 0.5 mg∙L-1 NAA (1-naphthaleneacetic acid). The obtained plantlets were successfully acclimatized (89%) in a 4:1 mixture of peat and sand and they flowered the following year. Presented protocol enables successful in vitro propagation of D. serotinus.

scholarly journals In vitro propagation of Cyrtopodium saintlegerianum rchb. f. (orchidaceae), a native orchid of the Brazilian savannah

2015 ◽  
Vol 15 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Lennis Afraire Rodrigues ◽  
Vespasiano Borges de Paiva Neto ◽  
Amanda Galdi Boaretto ◽  
Janaína Fernanda de Oliveira ◽  
Mateus de Aguiar Torrezan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Leaf Sheath ◽  
Naphthaleneacetic Acid ◽  
Brazilian Savannah ◽  
In Vitro Shoots ◽  
Shoot Number

In order to enable production of large quantities of plantlets for reintroduction programs, as well as economic exploration, Cyrtopodium saintlegerianum seeds were sown on Knudson culture medium. After seed germination, the protocorms were inoculated on Knudson culture medium supplemented with 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). The obtained shoots were individually inoculated in Knudson supplemented with gibberellic acid (GA3) in order to promote elongation. Seedlings were evaluated and then transplanted into trays containing commercial substrate Plantmax(r)-HT, or crushed Acuri leaf sheath. Auxin/cytokinin ratio influenced in vitro propagation of C. saintlegerianum, resulting in increased shoot number when 2.0 mg L-1 BA was added to the culture medium in the absence or presence of 0.5 mg L-1 NAA. This species proved to be promising for massal in vitro multiplication. Despite having incremented in vitro shoots elongation, the use of GA3 is unnecessary since it contributed negatively in the acclimatization of plants.

scholarly journals In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

2021 ◽  
Vol 43 ◽  
pp. e52866
Author(s):  
Karen Cristina Fialho dos Santos ◽  
Deyse Maria de Souza Silveira ◽  
Antônio da Silva Souza ◽  
Jucieny Ferreira de Sá ◽  
Carlos Alberto da Silva Ledo ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Culture Medium ◽  
Culture Technique ◽  
Naphthaleneacetic Acid ◽  
In Vitro Growth ◽  
Mineral Salts ◽  
In Vitro Multiplication ◽  
Aseptic Culture

In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff.

scholarly journals Effects of Sucrose and Other Additives on In Vitro Growth and Development of Purple Coneflower (Echinacea purpurea L.)

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Dahanayake Nilanthi ◽  
Yue-Sheng Yang
Keyword(s):  
Plant Growth ◽  
Culture Medium ◽  
Respiratory Infections ◽  
Shoot Multiplication ◽  
Echinacea Purpurea ◽  
Naphthaleneacetic Acid ◽  
Acid Plant ◽  
Purple Coneflower ◽  
Upper Respiratory

Echinacea purpurea (purple coneflower) is being used for the preparation of more than 240 extracts, salves, and tinctures to help cure diseases like rabies, cold, and upper respiratory infections. Hence, efforts were made to develop a culture medium for successful in vitro culturing of cornflower and to regenerate buds and induce roots to enable mass propagation of selected clones. Of the three levels of sucrose tested as a supplement to MS media (Murashige and Skoog’s medium, 1962) 3% showed better rooting of buds and appeared morphologically normal and identical as compared to those grown at higher and lower concentrations (2 and 4%). The additives hydrolyzed lactabumin (0.0, 100, 300, and 900 mgL−1), peptone (0.0, 100, 300, and 900 mgL−1), and yeast (0.0, 100, 300, and 900 mgL−1) to media containing 0.3 mgL−1 BA (6-benzyladenine) and 0.01 mgL−1 NAA (naphthaleneacetic acid-plant growth regulators) has negatively influenced proliferation of shoots. The higher concentrations of the above have delayed the development of plantlets. Shoot multiplication was enhanced by coconut water with 2% being the best among 4 and 8% tested. Shoot organogenesis was not influenced by copper sulphate (0, 1.5, 3, 6, and 12 mgL−1) and silver nitrate (0.0, 0.5, 2.5, and 12.5 mgL−1) supplements and at higher concentrations of the above inhibited plant growth.

scholarly journals In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

2017 ◽  
Vol 45 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Constantinos SALIS ◽  
Ioannis E. PAPADAKIS ◽  
Spyridon KINTZIOS ◽  
Marianna HAGIDIMITRIOU
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Root Length ◽  
In Vitro Propagation ◽  
Genetic Relationships ◽  
Shoot Proliferation ◽  
Poncirus Trifoliata ◽  
Naphthaleneacetic Acid ◽  
Issr Molecular Markers

The behavior of six citrus rootstocks, Volkameriana, Citrumelo ‘Swingle’, Citrange ‘Carrizo’, Poncirus trifoliata ‘Serra’, Poncirus trifoliata ‘Rubidoux’ and Poncirus trifoliata ‘Flying Dragon’, in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock ‘Swingle’ showed a higher number of roots per explant (4.2) followed by ‘Flying Dragon’ (3.93) and ‘Carrizo’ (3.23) rootstocks. The rootstocks ‘Swingle’ (140.8 mm), Volkameriana (148 mm) and ‘Flying Dragon’ (131.12 mm) had significantly higher root length per explant compared to ‘Carrizo’ (31 mm) and ‘Rubidoux’ (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata ‘Serra’ and Citrumelo ‘Swingle’) and 0.60 (Volkameriana and Citrumelo ‘Swingle’). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

scholarly journals Regeneration of Plants from Apical Meristem Tips and Nodal Segments of Arachis pintoi

2003 ◽  
Vol 30 (2) ◽  
pp. 75-79 ◽  
Author(s):  
H. Y. Rey ◽  
L. A. Mroginski
Keyword(s):  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Regeneration Potential ◽  
Arachis Pintoi ◽  
Solid Media ◽  
One Step

Abstract The in vitro regeneration potential of shoot apical tips (2 to 3 mm in length), meristems (0.3 to 0.5 mm in length), and nodal segments (4 to 7 mm long with an axillary bud) of diploid (2n = 2x = 20) and triploid (2n = 3x = 30) cytotypes of Arachis pintoi was evaluated. Explants were cultured on MS medium supplemented with different concentrations and combinations of naphthaleneacetic acid (NAA) and benzyladenine (BA). In one experiment the effect of gibberellic acid was tested. The cultures were done in liquid and solid media. Plant regeneration can be readily achieved from all explants in one step of 30 d culture on MS + 0.01 mg/L each of NAA and BA or two steps consisting of 1) shoots regeneration through culture of explants on MS + 0.01 mg/L each of NAA and BA, and 2) induction of rooting in regenerated shoots by reculture on MS + 0.01 mg/L NAA. The plantlets were successfully transferred to pots in a greenhouse.

In vitro propagation of Crambe maritima

1987 ◽  
Vol 65 (1) ◽  
pp. 72-75 ◽  
Author(s):  
J. Y. Peron ◽  
E. Regnier
Keyword(s):  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Petiole Explants ◽  
Acclimatization Period

A method for rapid micropropagation of sea kale (Crambe maritima L.) was developed. Petiole explants placed in vitro on a medium containing 0.5 mg/L indoleacetic acid (IAA), 6.0 mg/L kinetin, and 1.5 mg/L benzylaminopurine developed callus within 15 days and shoots within 28 days. Nearly four adventitious shoots could be developed within 3 weeks by placing the initial shoot on media without IAA. To develop roots, the shoots were then transferred to the basal medium containing 0.1 to 1.0 mg/L indolbutyric or α-naphthaleneacetic acid. Rooted plantlets were obtained within 2 or 3 weeks. After an acclimatization period of 6 weeks in a greenhouse in unsterilized medium, the plantlets could be set outdoors.

Biotechnological methods of in vitro propagation in willows (Salix spp.)

2012 ◽  
Vol 7 (5) ◽  
pp. 931-940 ◽  
Author(s):  
Dagmar Skálová ◽  
Božena Navrátilová ◽  
Lenka Richterová ◽  
Michal Knit ◽  
Michal Sochor ◽  
...  
Keyword(s):  
Central Europe ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Reproductive Potential ◽  
Ovule Culture ◽  
Nodal Segments ◽  
Young Shoot ◽  
Fragmented Populations ◽  
Salix Spp

AbstractMany populations of high-mountainous relic dioecious willows in Central Europe only consist of female individuals and are thus limited in their reproductive potential. We completed micropropagation experiments with shoot apexes and nodal segments of common and endangered willow (Salix) species, which can help to reintroduce autochthonous genotypes to their natural sites. Until recently, cultivation of green young shoot apexes of S. alba and S. lapponum showed the highest percentage of regeneration. We successfully applied the two-times-sterilisation due to high contamination of natural explants. The OK medium was the most efficient culture medium. In vitro propagation of willows with unisexual catkins, anther and ovule cultures were tested and optimised. Isolated anthers were cultivated on selected media and then microcallus and calluses of S. caprea and calluses of S. viminalis were formed on the A medium. Among various tested and optimised media for the ovule culture, the CP medium was the most efficient one. In this case, only the microcalluses of S. viminalis were observed. We developed biotechnological procedures that can be useful in conserving fragmented populations of high-mountainous willows.

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