scholarly journals Weak Static and Extremely Low Frequency Magnetic Fields AffectIn VitroPollen Germination

2011 ◽  
Vol 11 ◽  
pp. 875-890 ◽  
Author(s):  
Lucietta Betti ◽  
Grazia Trebbi ◽  
Fabio Fregola ◽  
Michela Zurla ◽  
Pietro Mesirca ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Growth Medium ◽  
Pollen Germination ◽  
Copper Wire ◽  
Germination Rate ◽  
Low Frequency ◽  
Biological Marker ◽  
Inhibitory Effect ◽  
Extremely Low Frequency

This study concerns the effects of a weak static magnetic field (MF) at 10 μT oriented downward, combined with a 16-Hz sinusoidal MF (10 μT), onin vitropollen germination of kiwifruit (Actinidia deliciosa). Extremely low frequency magnetic field (ELF-MF) exposure was carried out by a signal generator unit connected to a copper wire solenoid, inside which samples where placed. Two different kinds of treatment were performed: direct and indirect. In the direct treatment, pollen samples were directly exposed during rehydration, germination, or both. In the indirect treatment, the pollen growth medium was prepared with water aliquots (at standard temperature of 20°C and pH = 6.74) that were exposed before use for 8 or 24 h. The main purpose of our research was to identify a biological marker (in vitropollen germination in a stressing growth medium without Ca2+) susceptible to the effects of direct or indirect ELF-MF exposure. The working variable was the pollen germination rate, as detected blind after 3 h 30 min by an Axioplan microscope. A directionally consistent recovery of germination percentage was observed both for direct exposure (during germination and both rehydration and germination phases) and water-mediated exposure (with water exposed for 24 h and immediately used). Our results suggest that the ELF-MF treatment might partially remove the inhibitory effect caused by the lack of Ca2+in the culture medium, inducing a release of internal Ca2+stored in the secretory vesicles of pollen plasma membrane. Although preliminary, findings seem to indicate thein vitropollen performance as adequate to study the effects of ELF-MFs on living matter.

Effects of 50Hz extremely low frequency magnetic field on the morphology and function of boar spermatozoa capacitated in vitro

2007 ◽  
Vol 67 (4) ◽  
pp. 801-815 ◽  
Author(s):  
N. Bernabò ◽  
E. Tettamanti ◽  
M.G. Pistilli ◽  
D. Nardinocchi ◽  
P. Berardinelli ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Low Frequency ◽  
Extremely Low Frequency ◽  
Frequency Magnetic Field ◽  
And Function ◽  
Boar Spermatozoa

Early Apoptosis Difference and Mechanism of Hepatic Cell Lines Treated by Extremely Low Frequency Altering Electro-Magnetic Field Combined with Magnetic Nano Fe3O4 Particles In Vitro

2014 ◽  
Vol 556-562 ◽  
pp. 539-546
Author(s):  
Zhi Qiang Chen ◽  
Jian Wen ◽  
Hua Shang ◽  
Yuan Tang ◽  
Zheng Fang
Keyword(s):  
Magnetic Field ◽  
Tumor Cells ◽  
Low Frequency ◽  
Cellular Membrane ◽  
Extremely Low Frequency ◽  
Electro Magnetic Field ◽  
Hepatic Cells ◽  
Early Apoptosis ◽  
Electro Magnetic

Extremely low frequency electro-magnetic field (ELFF) exposure could induce hepatoma Bel-7402 and SMMC-7721 cells lines into early apoptosis without some significant effects to healthy hepatic cells HL-7702 cells in low exposure dose through affecting cellular membrane ion metabolism. Magnetic nanoFe3O4 particles (MNPs) presence with low effects to all hepatic cells lines strengthened ELFF bio-effects to tumor cells inducing more amount of tumor cells into early apoptosis with low effects to HL-7702 cells. Western-blot results indicated the early apoptosis signal channel Bcl protein family of tumor cells was activated significantly more effectively than HL-7702 cells due to higher MNPs uptake ratio of tumor cells and higher cellular membrane ion exchanging current than HL-7702 cells. Patch-clamp results showed MNPs combined with ELFF treatment inducing cells into early apoptosis should be partially based on ion metabolism disturbance of cells with similar to ELFF bio-effects to cells.

A Study on Early Apoptosis of Hepatoma Bel-7402 Cells In Vitro Treated by Altering-Electric Magnetic Field Exposure of Extremely Low Frequency Combined with Magnetic Nano-Fe3O4 Powders

2013 ◽  
Vol 364 ◽  
pp. 742-748 ◽  
Author(s):  
Zhi Qiang Chen ◽  
Jian Wen ◽  
Wen Yong Tu ◽  
Li Xiao ◽  
Zheng Fang
Keyword(s):  
Magnetic Field ◽  
Cell Metabolism ◽  
Low Frequency ◽  
Control Group ◽  
Cell Physiology ◽  
Field Exposure ◽  
Extremely Low Frequency ◽  
Significant Difference ◽  
Early Apoptosis

An investigation was made on the early apoptosis of hepatoma Bel-7402 cells, which were cultured on the magnetic nanoFe3O4 powders in different diameters (MNPs) in vitro, by an altering electro-magnetic field (ELFF) exposure of extremely low frequency. MNPs would strengthen the ELFF effects on the cell metabolism, and the effects were dependent on the diameter of powders. The experiments showed that MNPs could not influence the cell physiology obviously. However, ELFF exposure would inhibit the cell proliferation because some cells were arrested at G0/G1 period and the early apoptosis was induced with significant difference (P<0.05) as compared with the control group. The combination of MNPs with ELFF could strengthen the ratio of cells arrested at G0/G1 period and induced early apoptosis. As compared with the control group and the radiation group by ELFF with effects to cells ion metabolism, the significant difference was also present (P<0.05). The western-blot and patch-clamp experiments showed that the mechanism on the combined method was the same as that of the single ELFF exposure and treated by 40nm MNPs could be induced more cells into early apoptosis than those by the 80nm powders combined with ELFF.

Effect of 300 mT static and 50 Hz 0.1 mT extremely low frequency magnetic fields on Tuber borchii mycelium

2012 ◽  
Vol 58 (10) ◽  
pp. 1174-1182 ◽  
Author(s):  
Lucia Potenza ◽  
Roberta Saltarelli ◽  
Emanuela Polidori ◽  
Paola Ceccaroli ◽  
Antonella Amicucci ◽  
...  
Keyword(s):  
Gene Expression ◽  
Magnetic Field ◽  
Low Frequency ◽  
Hyphal Growth ◽  
In Vitro Cultivation ◽  
Tuber Borchii ◽  
Extremely Low Frequency ◽  
Biochemical Analyses ◽  
Set Up

The present work aimed to investigate whether exposure to static magnetic field (SMF) and extremely low frequency magnetic field (ELF-MF) can induce biomolecular changes on Tuber borchii hyphal growth. Tuber borchii mycelium was exposed for 1 h for 3 consecutive days to a SMF of 300 mT or an ELF-MF of 0.1 mT 50 Hz. Gene expression and biochemical analyses were performed. In mycelia exposed to ELF-MF, some genes involved in hyphal growth, investigated using quantitative real-time polymerase chain reaction, were upregulated, and the activity of many glycolytic enzymes was increased. On the contrary, no differences were observed in gene expression after exposure to SMF treatment, and only the activities of glucose 6-phosphate dehydrogenase and hexokinase increased. The data herein presented suggest that the electromagnetic field can act as an environmental factor in promoting hyphal growth and can be used for applicative purposes, such as the set up of new in vitro cultivation techniques.

scholarly journals Pollen of Arbutus unedo: Effects of plant growth regulators

2020 ◽  
Vol 44 (1) ◽  
pp. 55-59
Author(s):  
Zeliha Gökbayrak ◽  
Hakan Engin ◽  
Arda Akçal ◽  
Hatice Kiraz
Keyword(s):  
Growth Medium ◽  
Growth Regulators ◽  
Pollen Germination ◽  
Germination Rate ◽  
Pollen Grains ◽  
Combined Treatments ◽  
Arbutus Unedo ◽  
Antioxidant Characteristics

Arbutus unedo is a species mainly used for landscaping purposes and lately for honey production. Its antioxidant characteristics have also been a subject of of research interest. The germination of pollen grains freshly collected from flowers was tested in vitro, and 30% sucrose gave the highest pollen germination. Subsequently, three growth regulators belonging to the categories of gibberellins and brassinosteroids [24-epibrassinolide (Ebl) and 22S,23S-homobrassinolide (Hbl)] were added to the growth medium singly or in double combinations (gibberellin + brassinosteroid), and the petri dishes were kept for 24 hours at 26?1?C. The results showed that the highest germination rates were obtained from treatments with 0.01 ppm Hbl (45.47%) and 0.001 ppm Hbl (26.74%). They were followed by treatments with 0.001 ppm Ebl, 25 ppm GA3 and 0.1 ppm Ebl. As the concentration of GA3 increased, the germination rate declined considerably. Statistical analysis of the combined treatments showed that combinations of growth regulators lowered the germination rates compared to their individual application. Moreover, action of the lowest GA3 concentration (25 ppm) best matched the effects of brassinosteroids, and increasing the GA3 concentration in combined treatments did not improve germination. The highest germination was obtained with 0.1 ppm Hbl, followed by all Ebl treatments. Brassinosteroids might be an inducer of pollen germination in plants depending on their type and concentrations. When individually included in the growth medium, the extent of this induction was greater with the homobrassinolide than with the epibrassinolide. Their interactions with gibberellins are shown to be mostly dependent on the concentration of gibberellic acid used. The tested epibrassinolide was more consistent than the homobrassinolide in maintaining higher germination levels. The findings of this study indicate that gathering more information from studies involving other plant species is needed to clarify the role of brassinosteroids in in vitro and in vivo germination.

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