Multiple P450s and Variation in Neuronal Genes Underpins the Response to the Insecticide Imidacloprid in a Population of Drosophila melanogaster

AbstractInsecticide resistance is an economically important example of evolution in response to intense selection pressure. Here, the genetics of resistance to the neonicotinoid insecticide imidacloprid is explored using the Drosophila Genetic Reference Panel, a collection of inbred Drosophila melanogaster genotypes derived from a single population in North Carolina. Imidacloprid resistance varied substantially among genotypes, and more resistant genotypes tended to show increased capacity to metabolize and excrete imidacloprid. Variation in resistance level was then associated with genomic and transcriptomic variation, implicating several candidate genes involved in central nervous system function and the cytochrome P450s Cyp6g1 and Cyp6g2. CRISPR-Cas9 mediated removal of Cyp6g1 suggested that it contributed to imidacloprid resistance only in backgrounds where it was already highly expressed. Cyp6g2, previously implicated in juvenile hormone synthesis via expression in the ring gland, was shown to be expressed in metabolically relevant tissues of resistant genotypes. Cyp6g2 overexpression was shown to both metabolize imidacloprid and confer resistance. These data collectively suggest that imidacloprid resistance is influenced by a variety of previously known and unknown genetic factors.

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Defining the contribution of select neuropeptides and their receptors in regulating sesquiterpenoid biosynthesis by Drosophila melanogaster ring gland/corpus allatum through RNAi analysis

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2011.12.039 ◽

2012 ◽

Vol 176 (3) ◽

pp. 347-353 ◽

Cited By ~ 13

Author(s):

Christine Wang ◽

Jinrui Zhang ◽

Stephen S. Tobe ◽

William G. Bendena

Keyword(s):

Drosophila Melanogaster ◽

Corpus Allatum ◽

Ring Gland

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Identification of the Telomere elongation mutation in Drosophila

10.7287/peerj.preprints.26708 ◽

2018 ◽

Author(s):

Hemakumar M. Reddy ◽

Thomas A. Randall ◽

Radmila Capkova Frydrychova ◽

James M. Mason

Keyword(s):

Drosophila Melanogaster ◽

Next Generation Sequencing ◽

Ltr Retrotransposons ◽

Wild Type ◽

Dominant Mutation ◽

Site Specific ◽

Site Specific Recombination ◽

Telomere Elongation ◽

Drosophila Genetic Reference Panel ◽

Generation Sequencing

Background. Telomeres in Drosophila melanogaster are similar to those of other eukaryotes in terms of their function, although they are formed by non-LTR retrotransposons instead of telomerase-based short repeats. The length of the telomeres in Drosophila depends on the number of copies of these transposable elements. A dominant mutation, Tel1, causes a several-fold elongation of telomeres. Methods. In this study we identified the Tel1 mutation by a combination of transposon-induced, site-specific recombination and next generation sequencing. Results. Recombination located Tel1 to a 15 kb region in 92A. Comparison of the DNA sequence in this region with the Drosophila Genetic Reference Panel of wild type genomic sequences delimited Tel1 to a 3 bp deletion inside intron 8 of Ino80. Discussion. The mapped Tel1 mutation (3-bp deletion found in Ino80) did not appear to affect the quantity or length of the Ino80 transcript. Tel1 causes a significant reduction in transcripts of CG18493, a gene nested in an intron 8 of Ino80, which is expressed in ovaries and expected to encode a serine-type peptidase.

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Ecdysteroid synthesis by the ring gland of Drosophila melanogaster during late-larval, prepupal and pupal development

Journal of Insect Physiology ◽

10.1016/0022-1910(83)90107-5 ◽

1983 ◽

Vol 29 (1) ◽

pp. 65-71 ◽

Cited By ~ 37

Author(s):

Christopher P.F. Redfern

Keyword(s):

Drosophila Melanogaster ◽

Pupal Development ◽

Ring Gland

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Mutations in insulin signaling pathway alter juvenile hormone synthesis in Drosophila melanogaster

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2005.02.009 ◽

2005 ◽

Vol 142 (3) ◽

pp. 347-356 ◽

Cited By ~ 138

Author(s):

Meng-Ping Tu ◽

Chih-Ming Yin ◽

Marc Tatar

Keyword(s):

Drosophila Melanogaster ◽

Juvenile Hormone ◽

Signaling Pathway ◽

Insulin Signaling ◽

Insulin Signaling Pathway ◽

Hormone Synthesis

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Characterizing dopaminergic neuron vulnerability using Genome-wide analysis

Genetics ◽

10.1093/genetics/iyab081 ◽

2021 ◽

Author(s):

Jacinta Davis ◽

Claire Da Silva Santos ◽

Narda Caudillo Zavala ◽

Nicholas Gans ◽

Daniel Patracuolla ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Dopaminergic Neuron ◽

Reference Panel ◽

Functional Validation ◽

Mutant Analysis ◽

Genome Wide Analysis ◽

Progressive Loss ◽

Genome Wide ◽

Drosophila Genetic Reference Panel ◽

The Brain

Abstract Parkinson’s Disease (PD) is primarily characterized by the loss of dopaminergic (DA) neurons in the brain. However, little is known about why DA neurons are selectively vulnerable to PD. To identify genes that are associated with DA neuron loss, we screened through 201 wild-caught populations of Drosophila melanogaster as part of the Drosophila Genetic Reference Panel (DGRP). Here we identify the top associated genes containing SNPs that render DA neurons vulnerable. These genes were further analyzed by using mutant analysis and tissue-specific knockdown for functional validation. We found that this loss of DA neurons caused progressive locomotor dysfunction in mutants and gene knockdown analysis. The identification of genes associated with the progressive loss of DA neurons should help to uncover factors that render these neurons vulnerable in PD, and possibly develop strategies to make these neurons more resilient.

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Spruce terpenes: expression and weevil resistance

Canadian Journal of Forest Research ◽

10.1139/x99-053 ◽

1999 ◽

Vol 29 (6) ◽

pp. 761-767 ◽

Cited By ~ 2

Author(s):

J R Nault ◽

J F Manville ◽

T S Sahota

Keyword(s):

Picea Glauca ◽

Picea Engelmannii ◽

White Pine ◽

Resistance Level ◽

Large Variability ◽

Pissodes Strobi ◽

Engelmann Spruce ◽

Highly Correlated ◽

Resistant Genotypes ◽

Better Than

White spruce (Picea glauca (Moench) Voss) and Engelmann spruce (Picea engelmannii Parry) parent trees were selected that were rated as highly resistant or highly susceptible to white pine weevil (Pissodes strobi Peck) attack, based upon attack measurements on their open-pollinated progeny. For each parent, levels of terpenes or other volatiles in leaf and bark were highly correlated within ramets and highly variable within progeny. For the ramets, levels of individual terpenes could not be used to predict resistance class because of their large variability. A multivariate model developed using the terpenes showing the greatest differences between resistant classes was found to predict the resistance levels of the original ortets well, with 15 of 16 parent trees correctly predicted using either leaf or bark chemicals. These models failed to predict the resistance level of the open-pollinated progeny. Discriminant analysis was also attempted by dividing the ramet data into independent sets and using each set to predict the other. Again, prediction was no better than chance. We conclude that there is no merit in using terpenes as a selection tool to find resistant genotypes amongst white and Engelmann spruce populations.

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Downregulation of the dopamine D2-like receptor in corpus allatum affects juvenile hormone synthesis in Drosophila melanogaster females

Journal of Insect Physiology ◽

10.1016/j.jinsphys.2011.12.006 ◽

2012 ◽

Vol 58 (3) ◽

pp. 348-355 ◽

Cited By ~ 21

Author(s):

N.Е. Gruntenko ◽

O.V. Laukhina ◽

E.V. Bogomolova ◽

E.K. Karpova ◽

P.N. Menshanov ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Juvenile Hormone ◽

Corpus Allatum ◽

Hormone Synthesis ◽

Dopamine D2

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In situ localization of the per clock protein during development of Drosophila melanogaster.

Molecular and Cellular Biology ◽

10.1128/mcb.8.12.5378 ◽

1988 ◽

Vol 8 (12) ◽

pp. 5378-5385 ◽

Cited By ~ 67

Author(s):

L Saez ◽

M W Young

Keyword(s):

Drosophila Melanogaster ◽

Biological Clock ◽

Biological Rhythms ◽

Adult Brain ◽

Genetic Studies ◽

Ring Gland ◽

Ultradian Rhythmicity ◽

The Brain ◽

Clock Protein

The per locus influences biological rhythms in Drosophila melanogaster. In this study, per transcripts and proteins were localized in situ in pupae and adults. Earlier genetic studies have demonstrated that per expression is required in the brain for circadian locomotor activity rhythms and in the thorax for ultradian rhythmicity of the Drosophila courtship song. per RNA and proteins were detected in a restricted group of cells in the eyes and optic lobes of the adult brain and in many cell bodies in the adult and pupal thoracic ganglia. per products were also found in the pupal ring gland complex, a tissue involved in rhythmic aspects of Drosophila development. Abundant expression was seen in gonadal tissue. No biological clock phenotypes have been reported for this tissue in any of the per mutants, per protein mapped to different subcellular locations in different tissues. The protein accumulated in or around nuclei in some cells and appeared to be cytoplasmic in others.

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