scholarly journals The Wsp intermembrane complex mediates metabolic control of the swim-attach decision of Pseudomonas putida

2020 ◽  
Author(s):  
Ángeles Hueso-Gil ◽  
Belén Calles ◽  
Víctor de Lorenzo
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Transcription Factors ◽  
Pseudomonas Putida ◽  
Metabolic Control ◽  
Deletion Mutants ◽  
Similar Function ◽  
Complex Element ◽  
The Many ◽  
Mutant Phenotypes ◽  
S Genes

SUMMARYPseudomonas putida KT2440, a microorganism of interest for biotechnological purposes, is one amongst the many bacteria that attach to surfaces and produce biofilm. Although other mechanisms that contribute to this decision have been studied until now, a 7-genes-operon with a disposition and homology shared with the wsp operon in Pseudomonas aeruginosa remained to be investigated. In this work, we characterized the function of P. putida wsp operon by the combination of deletion mutants with complementations with P. aeruginosa’s genes and with deletions of 3 other genes: the genes that code for the transcription factors fleQ and fleN and the flagellar movement regulator, fglZ. Examining mutant behaviour at 6 and 24 hours under three different carbon regimes (citrate, glucose and fructose) we saw that this complex carries out a similar function in both Pseudomonas. In P. putida, the key components are WspR, a protein that harbours the domain for producing c-di-GMP, and WspF, which controls its activity. Transformation with the equivalent proteins of P. aeruginosa had a significant impact on of P. putida mutant phenotypes and could complement their functions under some conditions. These results contribute to the deeper understanding of the complex element network that regulate lifestyle decision in P. putida

Biodegradation of chlorophenols by immobilized pure-culture microorganisms

1996 ◽  
Vol 34 (10) ◽  
pp. 67-72 ◽  
Author(s):  
Lu Chih-Jen ◽  
Lee Chi-Mei ◽  
Huang Chiou-Zong
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Pure Culture ◽  
The Other ◽  
Batch Reactors ◽  
Agrobacterium Radiobacter ◽  
Culture Cells ◽  
Lag Period

The biodegradation of phenol and chlorophenols by immobilized pure-culture cells was conducted by a series of batch reactors. The microorganisms used in this study were Pseudomonas putida, Psuedomonas testosteroni, Pseudomonas aeruginosa, and Agrobacterium radiobacter. All four species showed the ortho-cleavage pathway to metabolize chlorophenols. Among the four species, P. testosteroni, P. putida, and P. aeruginosa could effectively remove phenol at 200 mg/l. P. testosteroni could effectively remove 2-chlorophenol at 10mg/l. However, the other three species, P. putida, P. aeruginosa, and A. radiobacter, could not effectively remove 2-chlorophenol. Although 3-chlorophenol is a recalcitrant compound, P. testosteroni also could rapidly metabolize 3-chlorophenol at 10 mg/l. The removal of 4-chlorophenol at 10 mg/l by P. testosteroni reached 98% within one day. P. aeruginosa and A. radiobacter also could metabolize 4-chlorophenol after 2 and 7 days of lag period, respectively.

scholarly journals High‐throughput dilution‐based growth method enables time‐resolved exo‐metabolomics of Pseudomonas putida and Pseudomonas aeruginosa

2021 ◽  
Author(s):  
Bjarke H. Pedersen ◽  
Nicolás Gurdo ◽  
Helle Krogh Johansen ◽  
Søren Molin ◽  
Pablo I. Nikel ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
High Throughput ◽  
Time Resolved ◽  
Growth Method

scholarly journals ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters

Microbiology ◽  
2009 ◽  
Vol 155 (4) ◽  
pp. 1080-1092 ◽  
Author(s):  
A. A. Bartosik ◽  
J. Mierzejewska ◽  
C. M. Thomas ◽  
G. Jagura-Burdzy
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fluorescence Microscopy ◽  
Bacterial Growth ◽  
Complete Loss ◽  
Physiological Parameters ◽  
Wild Type ◽  
Partial Removal ◽  
Partner Protein ◽  
The One ◽  
Mutant Phenotypes

Deletions leading to complete or partial removal of ParB were introduced into the Pseudomonas aeruginosa chromosome. Fluorescence microscopy of fixed cells showed that ParB mutants lacking the C-terminal domain or HTH motif formed multiple, less intense foci scattered irregularly, in contrast to the one to four ParB foci per cell symmetrically distributed in wild-type P. aeruginosa. All parB mutations affected both bacterial growth and swarming and swimming motilities, and increased the production of anucleate cells. Similar effects were observed after inactivation of parA of P. aeruginosa. As complete loss of ParA destabilized its partner ParB it was unclear deficiency of which protein is responsible for the mutant phenotypes. Analysis of four parB mutants showed that complete loss of ParB destabilized ParA whereas three mutants that retained the N-terminal 90 aa of ParB did not. As all four parB mutants demonstrate the same defects it can be concluded that either ParB, or ParA and ParB in combination, plays an important role in nucleoid distribution, growth and motility in P. aeruginosa.

scholarly journals Conjugal TOL Transfer from Pseudomonas putida to Pseudomonas aeruginosa: Effects of Restriction Proficiency, Toxicant Exposure, Cell Density Ratios, and Conjugation Detection Method on Observed Transfer Efficiencies

2005 ◽  
Vol 71 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Catalina Arango Pinedo ◽  
Barth F. Smets
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Strong Dependence ◽  
Plasmid Transfer ◽  
Mass Action ◽  
Tol Plasmid ◽  
Toxicant Exposure ◽  
Transfer Frequency ◽  
Cell Densities

ABSTRACT The effects of restriction proficiency and premating exposure to toxicants on conjugal transfer of the TOL plasmid between Pseudomonas spp. was investigated by examinations of filter matings. A Pseudomonas putida KT2442-derived strain carrying a gfp-tagged variant of the TOL plasmid was used as a donor, and both restriction-deficient (PAO1162N) and -proficient (PAO2002N) Pseudomonas aeruginosa strains were used as recipients. The in situ enumeration of conjugation events allowed us to obtain frequency estimates that were unbiased by transconjugant growth or plasmid retransfer. We observed a strong dependence of the plasmid transfer frequency on the initial donor-to-recipient ratio of surface matings, which invalidated the use of mass action-based plasmid transfer kinetic estimators. Careful control of the initial parental cell densities permitted evaluations of the true effects of restriction proficiency and toxicant exposure on TOL transfer. At standard donor-to-recipient ratios (10−3 for PAO1162N and 2 � 101 for PAO2002N) and total cell densities (105 cells/mm2 for PAO1162N and 106 cells/mm2 for PAO2002N), plasmid transfer frequencies without toxicant exposure were approximately 10−7 (events/mm2)−1 for PAO1162N and 10−11 (events/mm2)−1 for PAO2002N based on in situ observations of conjugation events. The enumeration of transconjugants via selective plating yielded transfer frequencies that were up to 1 order of magnitude lower. Premating exposure to sodium dodecyl sulfate (1 to 10 mM) significantly increased the transfer frequency for the restriction-proficient strain PAO2002N (P < 0.05) but not for the restriction-deficient strain PAO1162N. On the other hand, premating exposure to ethanol, toluene, or phenol had no positive effect on the plasmid transfer frequency. Clearly, restriction proficiency provides a strong barrier to interspecific transfer of the TOL plasmid, and this barrier was only marginally attenuated by recipient exposure to toxicants within the ranges examined.

THE POTENTIAL OF BACTERIA FOR THE MICROBIAL CONTROL OF GRASSHOPPERS AND LOCUSTS

1997 ◽  
Vol 129 (S171) ◽  
pp. 147-156 ◽  
Author(s):  
B. Zelazny ◽  
M.S. Goettel ◽  
B. Keller
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Thuringiensis ◽  
Serratia Marcescens ◽  
Schistocerca Gregaria ◽  
Locusta Migratoria ◽  
Microbial Control ◽  
Control Agent ◽  
Promising Agent ◽  
Laboratory Populations ◽  
The Many

AbstractBacteria have been implicated in disease epizootics observed in field populations and laboratory-reared locusts and grasshoppers. Two species [Serratia marcescens Bizio and Pseudomonas aeruginosa (Schroeter) Migula] consistently infect locusts when ingested with food and can spread in laboratory populations. However, research on developing these organisms for microbial control of locusts and grasshoppers begun in the 1950s has not been continued. In recent years strains of Bacillus thuringiensis Berliner have been studied for activity against locusts and grasshoppers. Results of additional trials by the authors are reported. Among 393 B. thuringiensis isolates and 93 preparations of other sporeforming bacteria fed to nymphs of Locusta migratoria (L.) and/or Schistocerca gregaria Forsk., none has shown any pathogenicity to the insects. The recent discovery of novel B. thuringiensis strains active against various diverse pests and the many properties of a sporeforming bacterium that satisfy the requirements for a microbial control agent, and the development of Serratia entomophila as a promising agent for control of grass grubs, provide incentive to continue the search for an orthopteran-active sporeforming bacterium and to re-investigate the potential of non-sporeforming bacterial pathogens as microbial control agents of grasshoppers and locusts.

scholarly journals nalD Encodes a Second Repressor of the mexAB-oprM Multidrug Efflux Operon of Pseudomonas aeruginosa

2006 ◽  
Vol 188 (24) ◽  
pp. 8649-8654 ◽  
Author(s):  
Yuji Morita ◽  
Lily Cao ◽  
Virginia C. Gould ◽  
Matthew B. Avison ◽  
Keith Poole
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Stenotrophomonas Maltophilia ◽  
Multidrug Efflux ◽  
Efflux System

ABSTRACT The Pseudomonas aeruginosa nalD gene encodes a TetR family repressor with homology to the SmeT and TtgR repressors of the smeDEF and ttgABC multidrug efflux systems of Stenotrophomonas maltophilia and Pseudomonas putida, respectively. A sequence upstream of mexAB-oprM and overlapping a second promoter for this efflux system was very similar to the SmeT and TtgR operator sequences, and NalD binding to this region was, in fact, demonstrated. Moreover, increased expression from this promoter was seen in a nalD mutant, consistent with NalD directly controlling mexAB-oprM expression from a second promoter.

scholarly journals Hierarchical management of carbon sources is regulated similarly by the CbrA/B systems in Pseudomonas aeruginosa and Pseudomonas putida

Microbiology ◽  
2014 ◽  
Vol 160 (10) ◽  
pp. 2243-2252 ◽  
Author(s):  
Martina Valentini ◽  
Sofía M. García-Mauriño ◽  
Isabel Pérez-Martínez ◽  
Eduardo Santero ◽  
Inés Canosa ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Nitrogen Availability ◽  
Carbon Sources ◽  
Signal Transduction Pathway ◽  
Exact Nature ◽  
Energy Status ◽  
Regulatory Systems ◽  
Close Relationship ◽  
Carbon Nitrogen Ratio

The CbrA/B system in pseudomonads is involved in the utilization of carbon sources and carbon catabolite repression (CCR) through the activation of the small RNAs crcZ in Pseudomonas aeruginosa, and crcZ and crcY in Pseudomonas putida. Interestingly, previous works reported that the CbrA/B system activity in P. aeruginosa PAO1 and P. putida KT2442 responded differently to the presence of different carbon sources, thus raising the question of the exact nature of the signal(s) detected by CbrA. Here, we demonstrated that the CbrA/B/CrcZ(Y) signal transduction pathway is similarly activated in the two Pseudomonas species. We show that the CbrA sensor kinase is fully interchangeable between the two species and, moreover, responds similarly to the presence of different carbon sources. In addition, a metabolomics analysis supported the hypothesis that CCR responds to the internal energy status of the cell, as the internal carbon/nitrogen ratio seems to determine CCR and non-CCR conditions. The strong difference found in the 2-oxoglutarate/glutamine ratio between CCR and non-CCR conditions points to the close relationship between carbon and nitrogen availability, or the relationship between the CbrA/B and NtrB/C systems, suggesting that both regulatory systems sense the same sort or interrelated signal.

Sign in / Sign up

Export Citation Format

Share Document