scholarly journals Genome-wide Association Studies Reveal Important Candidate Genes for the Bacillus pumilus TUAT-1-Arabidopsis thaliana Interaction

2020 ◽  
Author(s):  
Marina Soneghett Cotta ◽  
Fernanda Plucani do Amaral ◽  
Leonardo Magalhães Cruz ◽  
Roseli Wassem ◽  
Fábio de Oliveira Pedrosa ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Root Architecture ◽  
Bacillus Pumilus ◽  
Growth Promotion ◽  
Root Traits ◽  
Genome Wide Association ◽  
Genome Wide Association Studies ◽  
Genome Wide

ABSTRACTThe plant growth promoting bacterium (PGPB) Bacillus pumilus TUAT-1 is an indole acetic acid producer that can increase plant growth. Inoculation with this strain has been shown to confer greater plant tolerance to drought and saline conditions. Although the ability of TUAT-1 to enhance plant growth is well documented, little is known about what mechanisms underlie the plant response to this bacterium. Applying genome-wide association study (GWAS), we evaluated the interaction between TUAT-1 and Arabidopsis thaliana, screening 288 plant ecotypes for root architecture traits comparing non-inoculated and inoculated plants. Most of the ecotypes were significantly affected by TUAT-1 inoculation (66.7%) for at least one of the root traits measured. For example, some ecotypes responded positively increasing root growth while others showed reduced growth upon inoculation. A total of 96 ecotypes (33.3%) did not respond significantly to PGPB inoculation. These results are consistent with the widely reported strain-genotype specificity shown in many plant-microbe interactions. The GWAS analysis revealed significant SNPs associated to specific root traits leading to identification of several genes putatively involved in enabling the Bacillus pumilus TUAT-1 and A. thaliana association and contributing to plant growth promotion. Our results show that root architecture features are genetic separable traits associated with plant growth in association with TUAT-1. Our findings validate previous reported genes involved in Bacillus spp.-plant interaction, growth promotion and highlight potential genes involved in plant microbe interaction. We suggest that plant-bacterial interaction and the plant growth promotion are quantitative and multigenic traits. This knowledge expands our understanding of the functional mechanisms driving plant growth promotion by PGPB.

Endophytic colonization of Arabidopsis thaliana by Gluconacetobacter diazotrophicus and its effect on plant growth promotion, plant physiology, and activation of plant defense

2015 ◽  
Vol 399 (1-2) ◽  
pp. 257-270 ◽  
Author(s):  
A. L. S. Rangel de Souza ◽  
S. A. De Souza ◽  
M. V. V. De Oliveira ◽  
T. M. Ferraz ◽  
F. A. M. M. A. Figueiredo ◽  
...  
Keyword(s):  
Plant Physiology ◽  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Plant Defense ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Gluconacetobacter Diazotrophicus ◽  
Endophytic Colonization

scholarly journals Characterization of Selected Plant Growth-Promoting Rhizobacteria and Their Non-Host Growth Promotion Effects

2021 ◽  
Author(s):  
Di Fan ◽  
Donald L. Smith
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Content Type ◽  
Host Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

There are pressing needs to reduce the use of agrochemicals, and PGPR are receiving increasing interest in plant growth promotion and disease protection. This study follows up our previous report that the four newly isolated rhizobacteria promote the growth of Arabidopsis thaliana .

scholarly journals Plantibacter flavus, Curtobacterium herbarum, Paenibacillus taichungensis, and Rhizobium selenitireducens Endophytes Provide Host-Specific Growth Promotion of Arabidopsis thaliana, Basil, Lettuce, and Bok Choy Plants

2019 ◽  
Vol 85 (19) ◽  
Author(s):  
Evan Mayer ◽  
Patricia Dörr de Quadros ◽  
Roberta Fulthorpe
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Root Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Endophytes ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT A collection of bacterial endophytes isolated from stem tissues of plants growing in soils highly contaminated with petroleum hydrocarbons were screened for plant growth-promoting capabilities. Twenty-seven endophytic isolates significantly improved the growth of Arabidopsis thaliana plants in comparison to that of uninoculated control plants. The five most beneficial isolates, one strain each of Curtobacterium herbarum, Paenibacillus taichungensis, and Rhizobium selenitireducens and two strains of Plantibacter flavus were further examined for growth promotion in Arabidopsis, lettuce, basil, and bok choy plants. Host-specific plant growth promotion was observed when plants were inoculated with the five bacterial strains. P. flavus strain M251 increased the total biomass and total root length of Arabidopsis plants by 4.7 and 5.8 times, respectively, over that of control plants and improved lettuce and basil root growth, while P. flavus strain M259 promoted Arabidopsis shoot and root growth, lettuce and basil root growth, and bok choy shoot growth. A genome comparison between P. flavus strains M251 and M259 showed that both genomes contain up to 70 actinobacterial putative plant-associated genes and genes involved in known plant-beneficial pathways, such as those for auxin and cytokinin biosynthesis and 1-aminocyclopropane-1-carboxylate deaminase production. This study provides evidence of direct plant growth promotion by Plantibacter flavus. IMPORTANCE The discovery of new plant growth-promoting bacteria is necessary for the continued development of biofertilizers, which are environmentally friendly and cost-efficient alternatives to conventional chemical fertilizers. Biofertilizer effects on plant growth can be inconsistent due to the complexity of plant-microbe interactions, as the same bacteria can be beneficial to the growth of some plant species and neutral or detrimental to others. We examined a set of bacterial endophytes isolated from plants growing in a unique petroleum-contaminated environment to discover plant growth-promoting bacteria. We show that strains of Plantibacter flavus exhibit strain-specific plant growth-promoting effects on four different plant species.

Study of mechanisms for plant growth promotion elicited by rhizobacteria in Arabidopsis thaliana

2005 ◽  
Vol 268 (1) ◽  
pp. 285-292 ◽  
Author(s):  
Choong-Min Ryu ◽  
Chia-Hui Hu ◽  
Robert D. Locy ◽  
Joseph W. Kloepper
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion

scholarly journals Genome-wide analysis of bacterial determinants of plant growth promotion and induced systemic resistance byPseudomonas fluorescens

2017 ◽  
Vol 19 (11) ◽  
pp. 4638-4656 ◽  
Author(s):  
Xu Cheng ◽  
Desalegn W. Etalo ◽  
Judith E. van de Mortel ◽  
Ester Dekkers ◽  
Linh Nguyen ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Induced Systemic Resistance ◽  
Growth Promotion ◽  
Systemic Resistance ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Genome-Wide Association Study Reveals Key Genes for Differential Lead Accumulation and Tolerance in Natural Arabidopsis thaliana Accessions

2021 ◽  
Vol 12 ◽  
Author(s):  
Sílvia Busoms ◽  
Laura Pérez-Martín ◽  
Miquel Llimós ◽  
Charlotte Poschenrieder ◽  
Soledad Martos
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Walls ◽  
Association Studies ◽  
Root Cell ◽  
Genome Wide Association ◽  
Chromosome 1 ◽  
Genome Wide Association Studies ◽  
Genome Wide ◽  
Pb Accumulation ◽  
Root Cell Walls

Soil contamination by lead (Pb) has become one of the major ecological threats to the environment. Understanding the mechanisms of Pb transport and deposition in plants is of great importance to achieve a global Pb reduction. We exposed a collection of 360 Arabidopsis thaliana natural accessions to a Pb-polluted soil. Germination rates, growth, and leaf Pb concentrations showed extensive variation among accessions. These phenotypic data were subjected to genome wide association studies (GWAs) and we found a significant association on chromosome 1 for low leaf Pb accumulation. Genes associated with significant SNP markers were evaluated and we selected EXTENSIN18 (EXT18) and TLC (TRAM-LAG1-CLN8) as candidates for having a role in Pb homeostasis. Six Pb-tolerant accessions, three of them exhibiting low leaf Pb content, and three of them with high leaf Pb content; two Pb-sensitive accessions; two knockout T-DNA lines of GWAs candidate genes (ext18, tlc); and Col-0 were screened under control and high-Pb conditions. The relative expression of EXT18, TLC, and other genes described for being involved in Pb tolerance was also evaluated. Analysis of Darwinian fitness, root and leaf ionome, and TEM images revealed that Pb-tolerant accessions employ two opposing strategies: (1) low translocation of Pb and its accumulation into root cell walls and vacuoles, or (2) high translocation of Pb and its efflux to inactive organelles or intracellular spaces. Plants using the first strategy exhibited higher expression of EXT18 and HMA3, thicker root cell walls and Pb vacuolar sequestration, suggesting that these genes may contribute to the deposition of Pb in the roots. On the other hand, plants translocating high amounts of Pb showed upregulation of TLC and ABC transporters, indicating that these plants were able to properly efflux Pb in the aerial tissues. We conclude that EXT18 and TLC upregulation enhances Pb tolerance promoting its sequestration: EXT18 favors the thickening of the cell walls improving Pb accumulation in roots and decreasing its toxicity, while TLC facilitates the formation of dictyosome vesicles and the Pb encapsulation in leaves. These findings are relevant for the design of phytoremediation strategies and environment restoration.

Plant growth promotion and enhanced uptake of Cd by combinatorial application of Bacillus pumilus and EDTA on Zea mays L.

2020 ◽  
Vol 22 (13) ◽  
pp. 1372-1384
Author(s):  
Kashif Hayat ◽  
Saiqa Menhas ◽  
Jochen Bundschuh ◽  
Pei Zhou ◽  
Nabeel Khan Niazi ◽  
...  
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Bacillus Pumilus ◽  
Zea Mays L ◽  
Growth Promotion

scholarly journals Exploiting natural variation in crown root traits via genome-wide association studies in maize

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Houmiao Wang ◽  
Xiao Tang ◽  
Xiaoyi Yang ◽  
Yingying Fan ◽  
Yang Xu ◽  
...  
Keyword(s):  
Root System ◽  
Candidate Genes ◽  
System Architecture ◽  
Root Traits ◽  
Root System Architecture ◽  
Genome Wide Association ◽  
Genome Wide Association Studies ◽  
Crown Root ◽  
Genome Wide ◽  
Maize Varieties

Abstract Background Root system architecture (RSA), which is determined by the crown root angle (CRA), crown root diameter (CRD), and crown root number (CRN), is an important factor affecting the ability of plants to obtain nutrients and water from the soil. However, the genetic mechanisms regulating crown root traits in the field remain unclear. Methods In this study, the CRA, CRD, and CRN of 316 diverse maize inbred lines were analysed in three field trials. Substantial phenotypic variations were observed for the three crown root traits in all environments. A genome-wide association study was conducted using two single-locus methods (GLM and MLM) and three multi-locus methods (FarmCPU, FASTmrMLM, and FASTmrEMMA) with 140,421 SNP. Results A total of 38 QTL including 126 SNPs were detected for CRA, CRD, and CRN. Additionally, 113 candidate genes within 50 kb of the significant SNPs were identified. Combining the gene annotation information and the expression profiles, 3 genes including GRMZM2G141205 (IAA), GRMZM2G138511 (HSP) and GRMZM2G175910 (cytokinin-O-glucosyltransferase) were selected as potentially candidate genes related to crown root development. Moreover, GRMZM2G141205, encoding an AUX/IAA transcriptional regulator, was resequenced in all tested lines. Five variants were identified as significantly associated with CRN in different environments. Four haplotypes were detected based on these significant variants, and Hap1 has more CRN. Conclusions These findings may be useful for clarifying the genetic basis of maize root system architecture. Furthermore, the identified candidate genes and variants may be relevant for breeding new maize varieties with root traits suitable for diverse environmental conditions.

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