Transcriptomic analyses revealed the effect of Funneliformis mosseae on differentially expressed genes in Fusarium oxysporum

AbstractSoybean root rot is a typical soil-borne disease that severely affects the yield of soybean, and F. mosseae, the dominant strain of AMF in continuous cropping of soybean. The aim of this study was to providing an experimental basis for the study of the molecular mechanism underlying the alleviation of the obstacles associated with the continuous cropping of soybean by AMF. In this study, F. mosseae was inoculated in soil planted with soybean infected with F. oxysporum. The results showed that the incidence of soybean root rot was significantly reduced after inoculation with F. mosseae. The significantly upregulated genes encoded the ABC transporter, ATP-binding/permease protein and the ABC transporter, ATP-binding protein. The significantly downregulated genes encoded chitin-binding domain proteins; key enzymes involved in metabolic pathways such as glycolysis, including class II fructose-bisphosphate aldolase and NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, glycoside hydrolase family 61 protein, which hydrolyse cellulose and hemicellulose; actin and other major components of the cytoskeleton. The DEGs were enriched in antigen processing and presentation, carbon fixation in photosynthetic organisms, glycolysis/gluconeogenesis, the MAPK signalling pathway, protein processing in the endoplasmic reticulum and RNA degradation. Inoculation with F. mosseae could promote the growth and development of soybean and improve disease resistance. This study provides an experimental basis for further research on the molecular mechanism underlying the alleviation of challenges associated with the continuous cropping of soybean by AMF.

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A single power stroke by ATP binding drives substrate translocation in a heterodimeric ABC transporter

eLife ◽

10.7554/elife.55943 ◽

2020 ◽

Vol 9 ◽

Cited By ~ 9

Author(s):

Erich Stefan ◽

Susanne Hofmann ◽

Robert Tampé

Keyword(s):

Abc Transporter ◽

Antigen Processing ◽

Atp Hydrolysis ◽

Power Stroke ◽

Atp Binding ◽

Nucleotide Exchange ◽

Single Turnover ◽

Physiological Processes ◽

Chemomechanical Coupling ◽

Functional Homolog

ATP-binding cassette (ABC) transporters constitute the largest family of primary active transporters, responsible for many physiological processes and human maladies. However, the mechanism how chemical energy of ATP facilitates translocation of chemically diverse compounds across membranes is poorly understood. Here, we advance the quantitative mechanistic understanding of the heterodimeric ABC transporter TmrAB, a functional homolog of the transporter associated with antigen processing (TAP) by single-turnover analyses at single-liposome resolution. We reveal that a single conformational switch by ATP binding drives unidirectional substrate translocation. After this power stroke, ATP hydrolysis and phosphate release launch the return to the resting state, which facilitates nucleotide exchange and a new round of substrate binding and translocation. In contrast to hitherto existing steady-state assays, our single-turnover approach uncovers the power stroke in substrate translocation and the tight chemomechanical coupling in these molecular machines.

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Transcriptome and metabolite profiling reveals the effects of Funneliformis mosseae on the roots of continuously cropped soybeans

10.21203/rs.3.rs-31120/v1 ◽

2020 ◽

Author(s):

Cheng-Cheng Lu ◽

Na Guo ◽

Chao Yang ◽

Hai-Bing Sun ◽

Baiyan Cai

Keyword(s):

Root Rot ◽

Mycorrhizal Fungi ◽

Plant Stress ◽

Arbuscular Mycorrhizal ◽

Ultra Performance Liquid Chromatography ◽

Integrated Analysis ◽

Continuous Cropping ◽

Soybean Root ◽

Disease Resistant ◽

Coa Reductase

Abstract Background Arbuscular mycorrhizal fungi are the most widely distributed mycorrhizal fungi, which can form mycorrhizal symbionts with plant roots and enhance plant stress resistance by regulating host metabolic activities. In this paper, the RNA sequencing and ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) technologies were used to study the transcriptome and metabolite profiles of the roots of continuously cropped soybeans that were infected with F. mosseae and F. oxysporum. The objective was to explore the effects of F. mosseae treatment on soybean root rot infected with F. oxysporum. Results According to the transcriptome profiles, 24285 differentially expressed genes (DEGs) were identified, and the expression of genes encoding phenylalanine ammonia lyase (PAL), trans-cinnamate monooxygenase (CYP73A), cinnamyl-CoA reductase (CCR), chalcone isomerase (CHI) and coffee-coenzyme o-methyltransferase were upregulated after being infected with F. oxysporum; these changes were key to the induction of the soybean’s defence response. The metabolite results showed that daidzein and 7,4-dihydroxy, 6-methoxy isoflavone (glycine), which are involved in the isoflavone metabolic pathway, were upregulated after the roots were inoculated with F. mosseae. In addition, a substantial alteration in the abundance of amino acids, phenolic and terpene metabolites all led to the synthesis of defence compounds. An integrated analysis of the metabolic and transcriptomic data revealed that substantial alterations in the abundance of most of the intermediate metabolites and enzymes changed substantially under pathogen infection. These changes included the isoflavonoid biosynthesis pathway, which suggests that isoflavonoid biosynthesis plays an important role in the soybean root response. Conclusion The results showed that F. mosseae could alleviate the root rot caused by continuous cropping. The increased activity of some disease-resistant genes and disease-resistant metabolites may partly account for the ability of the plants to resist diseases. This study provides new insights into the molecular mechanism by which AMF alleviates soybean root rot, which is important in agriculture.

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D-helix influences dimerization of the ATP-binding cassette (ABC) transporter associated with antigen processing 1 (TAP1) nucleotide-binding domain

PLoS ONE ◽

10.1371/journal.pone.0178238 ◽

2017 ◽

Vol 12 (5) ◽

pp. e0178238 ◽

Cited By ~ 6

Author(s):

Ahmet S. Vakkasoglu ◽

Sriram Srikant ◽

Rachelle Gaudet

Keyword(s):

Abc Transporter ◽

Antigen Processing ◽

Nucleotide Binding ◽

Binding Domain ◽

Atp Binding ◽

Nucleotide Binding Domain ◽

Atp Binding Cassette

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Transcriptome and metabolite profiling reveals the effects of Funneliformis mosseae on the roots of continuously cropped soybeans

BMC Plant Biology ◽

10.1186/s12870-020-02647-2 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Cheng-Cheng Lu ◽

Na Guo ◽

Chao Yang ◽

Hai-Bing Sun ◽

Bai-Yan Cai

Keyword(s):

Root Rot ◽

Mycorrhizal Fungi ◽

Plant Stress ◽

Arbuscular Mycorrhizal ◽

Ultra Performance Liquid Chromatography ◽

Integrated Analysis ◽

Continuous Cropping ◽

Soybean Root ◽

Disease Resistant ◽

Coa Reductase

Abstract Background Arbuscular mycorrhizal fungi are the most widely distributed mycorrhizal fungi, which can form mycorrhizal symbionts with plant roots and enhance plant stress resistance by regulating host metabolic activities. In this paper, the RNA sequencing and ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) technologies were used to study the transcriptome and metabolite profiles of the roots of continuously cropped soybeans that were infected with F. mosseae and F. oxysporum. The objective was to explore the effects of F. mosseae treatment on soybean root rot infected with F. oxysporum. Results According to the transcriptome profiles, 24,285 differentially expressed genes (DEGs) were identified, and the expression of genes encoding phenylalanine ammonia lyase (PAL), trans-cinnamate monooxygenase (CYP73A), cinnamyl-CoA reductase (CCR), chalcone isomerase (CHI) and coffee-coenzyme o-methyltransferase were upregulated after being infected with F. oxysporum; these changes were key to the induction of the soybean’s defence response. The metabolite results showed that daidzein and 7,4-dihydroxy, 6-methoxy isoflavone (glycine), which are involved in the isoflavone metabolic pathway, were upregulated after the roots were inoculated with F. mosseae. In addition, a substantial alteration in the abundance of amino acids, phenolic and terpene metabolites all led to the synthesis of defence compounds. An integrated analysis of the metabolic and transcriptomic data revealed that substantial alterations in the abundance of most of the intermediate metabolites and enzymes changed substantially under pathogen infection. These changes included the isoflavonoid biosynthesis pathway, which suggests that isoflavonoid biosynthesis plays an important role in the soybean root response. Conclusion The results showed that F. mosseae could alleviate the root rot caused by continuous cropping. The increased activity of some disease-resistant genes and disease-resistant metabolites may partly account for the ability of the plants to resist diseases. This study provides new insights into the molecular mechanism by which AMF alleviates soybean root rot, which is important in agriculture.

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