scholarly journals An inexpensive semi-automated sample processing pipeline for cell-free RNA

2020 ◽  
Author(s):  
Mira N. Moufarrej ◽  
Stephen R. Quake
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Low Cost ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Sample Processing ◽  
Processing Pipeline ◽  
Liquid Handling ◽  
Sample Throughput ◽  
Automated Pipeline

AbstractDespite advances in automated liquid handling and microfluidics, preparing samples for RNA sequencing at scale generally requires expensive equipment, which is beyond the reach of many academic labs. Manual sample preparation remains a slow, expensive, and error-prone process. Here, we describe a low-cost, semi-automated pipeline to extract cell-free RNA (cfRNA) that increases sample throughput by 12-fold while reducing time spent and cost by nearly 11-fold and 3-fold respectively. This pipeline is generalizable for many nucleic acid extraction applications, thereby increasing the scale of studies, which can be performed in small research labs.

Rapid and automated sample preparation for nucleic acid extraction on a microfluidic CD (compact disk)

2006 ◽  
Author(s):  
Jitae Kim ◽  
Horacio Kido ◽  
Jim V. Zoval ◽  
Dominic Gagné ◽  
Régis Peytavi ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Acid Extraction ◽  
Compact Disk ◽  
Nucleic Acid Extraction ◽  
Automated Sample Preparation

scholarly journals Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Anja Gulliksen ◽  
Helen Keegan ◽  
Cara Martin ◽  
John O'Leary ◽  
Lars A. Solli ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Clinical Sample ◽  
Point Of Care ◽  
Fluorescent Detection ◽  
Clinical Samples ◽  
Microfluidic Channels ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Hpv E6

The paper presents the development of a “proof-of-principle” hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens. The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing. It consists of two modular prototypes, one for sample preparation and one for amplification and detection; however, a common interface is available to facilitate later integration into one single module. Nucleic acid extracts (n=28) from cervical cytology specimens extracted on the sample preparation chip were tested using the PreTect HPV-Proofer and achieved an overall detection rate for HPV across all dilutions of 50%–85.7%. A subset of 6 clinical samples extracted on the sample preparation chip module was chosen for complete validation on the NASBA chip module. For 4 of the samples, a 100% amplification for HPV 16 or 33 was obtained at the 1 : 10 dilution for microfluidic channels that filled correctly. The modules of a “sample-in, answer-out” diagnostic platform have been demonstrated from clinical sample input through sample preparation, amplification and final detection.

Design of Automatic Nucleic Acid Extraction Instrument Based on Magnetic Nanobeads

2012 ◽  
Vol 621 ◽  
pp. 313-316
Author(s):  
Yong Jie Dou ◽  
Jian Sheng Xu ◽  
Cong Liang Deng ◽  
Xiao Li Zhao ◽  
Leng Nie ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Room Temperature ◽  
Handling Time ◽  
Sample Volume ◽  
Sample Temperature ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Sample Handling ◽  
Sample Throughput ◽  
Magnetic Nanobeads

In this paper, an automatic nucleic acid extraction instrument is described. It consists of mechanical components, motor controlling module, temperature controlling module and computer. Also, a prototype has been designed and constructed. The maximum sample throughput of this prototype is 96, the sample volume is 300-1000ul, the maximum sample handling time is about 60min, the sample temperature controlling range is from room temperature to 95oC, and the accuracy of temperature controlling achieved is about ± 2oC.

Advances in Fully Automated Nucleic Acid Extraction System Based on Magnetic Nanobeads

2019 ◽  
Vol 11 (12) ◽  
pp. 1633-1643
Author(s):  
Zhu Chen ◽  
Changhu Xiao ◽  
Manling Tang ◽  
Yuyue Xu ◽  
Ziyu He ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Point Of Care ◽  
Low Cost ◽  
Future Trend ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Advantages And Disadvantages ◽  
Good Repeatability ◽  
Magnetic Nanobeads

Using magnetic nanobeads (MNBs) to extract nucleic acids is an efficient, inexpensive, easy automation, high throughput and good repeatability, instead of the traditional nucleic acid extraction (NAE) methods. Advances in fully automated MNBs-based nucleic acid extraction systems (MNAES) can push the frontiers of point-of-care testing (POCT) devices towards low-cost, automation, and enhanced accuracy molecular-level diagnostics. So, this paper introduces the pipettingbased MNAES with position of magnetic separation kit or tip, and based on magnetic bar MNAES with blending manner is shock or rotation. Further, advantages and disadvantages of various MNAES are compared. We envisage that the future trend in molecular diagnosis and monitoring will be facilitation, intelligent, miniaturization, and high throughput MNAES with sample-in-answer-out capability.

Nucleic acid extraction: Fundamentals of sample preparation methodologies, current advancements, and future endeavors

2020 ◽  
Vol 130 ◽  
pp. 115985
Author(s):  
Miranda N. Emaus ◽  
Marcelino Varona ◽  
Derek R. Eitzmann ◽  
Shu-An Hsieh ◽  
Victoria R. Zeger ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

Automated nucleic acid extraction from whole blood, B. subtilis, E. coli, and Rift Valley fever virus on a centrifugal microfluidic LabDisk

RSC Advances ◽  
2015 ◽  
Vol 5 (41) ◽  
pp. 32144-32150 ◽  
Author(s):  
O. Strohmeier ◽  
S. Keil ◽  
B. Kanat ◽  
P. Patel ◽  
M. Niedrig ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Whole Blood ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rna Virus ◽  
Rift Valley Fever Virus ◽  
Low Cost ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Valley Fever

We present total nucleic acid extraction from whole blood, Gram-positiveBacillus subtilis, Gram-negativeEscherichia coli, andRift Valley feverRNA virus on a low-cost, centrifugal microfluidic cartridge processed in a portable processing device.

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