Multifunctional Device for Nucleic Acid Extraction Based on Magnetic Separation and Its Co-Working with Liquid Handling System for High Throughput Sample Preparation

2016 ◽  
Vol 16 (7) ◽  
pp. 6919-6924 ◽  
Author(s):  
Yanqi Wu ◽  
Hui Chen ◽  
Zhu Chen ◽  
Libo Nie ◽  
Bin Liu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Magnetic Separation ◽  
High Throughput ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Handling System ◽  
Liquid Handling ◽  
Multifunctional Device

Design and Application of a High-Throughput Sample Processing Module Based on Magnetic Beads

2018 ◽  
Vol 10 (3) ◽  
pp. 320-328 ◽  
Author(s):  
Yile Fang ◽  
Yanqi Wu ◽  
Pei Liao ◽  
Zhu Chen ◽  
Hui Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Magnetic Separation ◽  
High Throughput ◽  
Magnetic Beads ◽  
Performance Evaluations ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Dc Power ◽  
Processing Module

One of key challenges in diagnosis of infectious diseases is collecting a large quantity of purified nucleic acids from pathogens immediately. To address this challenge, a high-throughput magnetic separation module and a heating and vibrating module were developed to optimize the operation of biological sample preparation (like nucleic acid extraction) on our home-built liquid handing system. Both of the two modules have 4 working locations for Biomolecular Screening (BS) standard labware, and three functions (magnetic separation, heating and vibrating) were elaborately integrated into the two modules. Each module has its own core control circuit and a unique interface port containing a 24 V DC power supply connector and a CAN bus communication connector. When equipping with the two modules, the home-built liquid handing system transformed into an integrated automatic sample preparation workstation. Performance evaluations were carried out on the two modules and finally a nucleic acid extraction of human's whole blood was carried out on the workstation. Results showed that the two modules could cooperate well with the liquid handing system, and the workstation exhibited its ability for high-throughput sample preparation.

scholarly journals An inexpensive semi-automated sample processing pipeline for cell-free RNA

2020 ◽  
Author(s):  
Mira N. Moufarrej ◽  
Stephen R. Quake
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Low Cost ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Sample Processing ◽  
Processing Pipeline ◽  
Liquid Handling ◽  
Sample Throughput ◽  
Automated Pipeline

AbstractDespite advances in automated liquid handling and microfluidics, preparing samples for RNA sequencing at scale generally requires expensive equipment, which is beyond the reach of many academic labs. Manual sample preparation remains a slow, expensive, and error-prone process. Here, we describe a low-cost, semi-automated pipeline to extract cell-free RNA (cfRNA) that increases sample throughput by 12-fold while reducing time spent and cost by nearly 11-fold and 3-fold respectively. This pipeline is generalizable for many nucleic acid extraction applications, thereby increasing the scale of studies, which can be performed in small research labs.

scholarly journals Evaluation of an Automated High-Throughput Liquid-Based RNA Extraction Platform on Pooled Nasopharyngeal or Saliva Specimens for SARS-CoV-2 RT-PCR

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 615
Author(s):  
Allen Wing-Ho Chu ◽  
Cyril Chik-Yan Yip ◽  
Wan-Mui Chan ◽  
Anthony Chin-Ki Ng ◽  
Dream Lok-Sze Chan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Rna Extraction ◽  
Nasopharyngeal Swab ◽  
Acid Extraction ◽  
Rt Pcr ◽  
Nucleic Acid Extraction ◽  
Suitable Alternative ◽  
Pcr Inhibitors ◽  
Pcr Targeting

SARS-CoV-2 RT-PCR with pooled specimens has been implemented during the COVID-19 pandemic as a cost- and manpower-saving strategy for large-scale testing. However, there is a paucity of data on the efficiency of different nucleic acid extraction platforms on pooled specimens. This study compared a novel automated high-throughput liquid-based RNA extraction (LRE) platform (PHASIFYTM) with a widely used magnetic bead-based total nucleic acid extraction (MBTE) platform (NucliSENS® easyMAG®). A total of 60 pools of nasopharyngeal swab and 60 pools of posterior oropharyngeal saliva specimens, each consisting of 1 SARS-CoV-2 positive and 9 SARS-CoV-2 negative specimens, were included for the comparison. Real-time RT-PCR targeting the SARS-CoV-2 RdRp/Hel gene was performed, and GAPDH RT-PCR was used to detect RT-PCR inhibitors. No significant differences were observed in the Ct values and overall RT-PCR positive rates between LRE and MBTE platforms (92.5% (111/120] vs 90% (108/120]), but there was a slightly higher positive rate for LRE (88.3% (53/60]) than MBTE (81.7% (49/60]) among pooled saliva. The automated LRE method is comparable to a standard MBTE method for the detection of SAR-CoV-2 in pooled specimens, providing a suitable alternative automated extraction platform. Furthermore, LRE may be better suited for pooled saliva specimens due to more efficient removal of RT-PCR inhibitors.

scholarly journals NEATTILL: A simplified procedure for nucleic acid extraction from arrayed tissue for TILLING and other high-throughput reverse genetic applications

Plant Methods ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 3 ◽  
Author(s):  
Yellamaraju Sreelakshmi ◽  
Soni Gupta ◽  
Reddaiah Bodanapu ◽  
Vineeta Chauhan ◽  
Mickey Hanjabam ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Acid Extraction ◽  
Reverse Genetic ◽  
Nucleic Acid Extraction ◽  
Simplified Procedure

scholarly journals Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation

PLoS Biology ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. e3000107 ◽  
Author(s):  
Phil Oberacker ◽  
Peter Stepper ◽  
Donna M. Bond ◽  
Sven Höhn ◽  
Jule Focken ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Magnetic Beads ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Open Platform

Inexpensive, High Throughput Microplate Format for Plant Nucleic Acid Extraction: Suitable for Multiplex Southern Analyses of Transgenes

Crop Science ◽  
2005 ◽  
Vol 45 (5) ◽  
pp. 1985-1989 ◽  
Author(s):  
L. Flagel ◽  
J. R. Christensen ◽  
C. D. Gustus ◽  
K. P. Smith ◽  
P. M. Olhoft ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

Rapid and automated sample preparation for nucleic acid extraction on a microfluidic CD (compact disk)

2006 ◽  
Author(s):  
Jitae Kim ◽  
Horacio Kido ◽  
Jim V. Zoval ◽  
Dominic Gagné ◽  
Régis Peytavi ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Acid Extraction ◽  
Compact Disk ◽  
Nucleic Acid Extraction ◽  
Automated Sample Preparation

scholarly journals High-throughput HTLV-1 proviral DNA detection system using a nucleic acid extraction robot and real-time PCR detection.

2001 ◽  
Vol 47 (3) ◽  
pp. 378-383 ◽  
Author(s):  
Chieko Matsumoto ◽  
Rieko Shiozawa ◽  
Shigeki Mitsunaga ◽  
Akiko Ichikawa ◽  
Rika Ishiwatari ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Detection System ◽  
Dna Detection ◽  
Pcr Detection ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Proviral Dna

scholarly journals A bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3853-3861 ◽  
Author(s):  
Chenguang Zhang ◽  
Gongchen Sun ◽  
Satyajyoti Senapati ◽  
Hsueh-Chia Chang
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
High Yield ◽  
Acid Extraction ◽  
Field Flow Fractionation ◽  
Nucleic Acid Extraction ◽  
Isolation And Purification ◽  
Extraction And Purification ◽  
Flow Fractionation ◽  
Continuous Field

We report a new Bifurcated Continuous Field-Flow Fractionation (BCFFF) microfluidic chip for isolation and purification of nucleic acids from blood plasma with high and concentration-independent yield. The platform is ideal for isolation and quantification of small miRNAs.

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