scholarly journals Ultrastructural view of astrocyte-astrocyte and astrocyte-synapse contacts within the hippocampus

2020 ◽  
Author(s):  
Conrad M. Kiyoshi ◽  
Sydney Aten ◽  
Emily P. Arzola ◽  
Jeremy A. Patterson ◽  
Anne T. Taylor ◽  
...  

SummaryAstrocytes branch out and make contact at their interfaces. However, the ultrastructural interactions of astrocytes and astrocytes with their surroundings, including the spatial-location selectivity of astrocyte-synapse contacts, remain unknown. Here, the branching architecture of three neighboring astrocytes, their contact interfaces, and their surrounding neurites and synapses have been traced and 3D reconstructed using serial block-face scanning electron microscopy (SBF-SEM). Our reconstructions reveal extensive reflexive, loop-like processes that serve as scaffolds to neurites and give rise to spongiform astrocytic morphology. At the astrocyte-astrocyte interface, a cluster of process-process contacts were identified, which biophysically explains the existence of low inter-astrocytic electrical resistance. Additionally, we found that synapses uniformly made contact with the entire astrocyte, from soma to terminal processes, and can be ensheathed by two neighboring astrocytes. Lastly, in contrast to densely packed vesicles at the synaptic boutons, vesicle-like structures were scant within astrocytes. Together, these ultrastructural details should expand our understanding of functional astrocyte-astrocyte and astrocyte-neuron interactions.

2021 ◽  
Vol 27 (S1) ◽  
pp. 3176-3177
Author(s):  
Nanami Takagi ◽  
Norio Yamashita ◽  
Yuki Tsujimura ◽  
Hiroshi Takemura ◽  
Sze Keat Chee ◽  
...  

2017 ◽  
Vol 23 (S1) ◽  
pp. 1266-1267 ◽  
Author(s):  
Barbara Armbruster ◽  
Christopher Booth ◽  
Stuart Searle ◽  
Michael Cable ◽  
Ronald Vane

2017 ◽  
Vol 130 (10) ◽  
pp. 1845-1855 ◽  
Author(s):  
Faye M. Nixon ◽  
Thomas R. Honnor ◽  
Nicholas I. Clarke ◽  
Georgina P. Starling ◽  
Alison J. Beckett ◽  
...  

Author(s):  
Justin A. Courson ◽  
Paul T. Landry ◽  
Thao Do ◽  
Eric Spehlmann ◽  
Pascal J. Lafontant ◽  
...  

BioTechniques ◽  
2014 ◽  
Vol 57 (6) ◽  
Author(s):  
Mohammed Yusuf ◽  
Bo Chen ◽  
Teruo Hashimoto ◽  
Ana Katrina Estandarte ◽  
George Thompson ◽  
...  

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