MSM and sesame seed oil improve hyperlipidemia and PUFA metabolism in the db/db mouse

BackgroundAn estimated 463 million people worldwide suffer from diabetes, and that number is projected to rise significantly to 700 million by 2045. One of the hallmarks of type 2 diabetes and metabolic syndrome is alterations in the lipid profile and polyunsaturated fatty acid metabolism.ObjectiveThe objective of this study was to identify lipid alterations in leptin receptor deficient BKS.Cg-Dock7m+/+ Leprdb/J (db/db) mice, a murine model of type 2 diabetes, for the purpose of establishing a baseline biological signature for the subsequent evaluation of natural products with purported lipid-altering activity.MethodsSix-week old male db/db mice (n = 10/group) were randomized to the following groups: 1) diabetic control with no treatment, 2) methylsulfonylmethane (MSM) treatment (3.81 ± 0.33 g/kg), 3) sesame seed oil (SSO) treatment (23.54 ± 2.91 mg/kg), and 4) MSM and SSO combination treatment. Eight-week old male C57BL6/J mice (n=10) were used as a non-diabetic control group.ResultsSerum triglycerides and total cholesterol were significantly increased in the db/db model compared to nondiabetic control, mimicking the diabetic condition in people. HDL-cholesterol was significantly increased in all db/db treatment groups, with the most significant treatment effect in the MSM and SSO combination group, with a corresponding decrease in non-HDL cholesterol (LDL and VLDL). With regard to fatty acid metabolism, serum total polyunsaturated fatty acid (PUFA) levels were significantly reduced in diabetic mice compared to control mice. In contrast, feeding of only SSO reversed this effect such that fed mice exhibited serum PUFA levels comparable to control mice.ConclusionsTreatment of db/db mice with MSM and SSO improved commonly measured clinical parameters in serum lipid panels. The combination of MSM and SSO treatment’s effects on HDL and non-HDL cholesterol and fatty acid metabolism could lead to improved clinical outcomes in people such as reduced incidence of atherosclerosis and hepatic steatosis.