scholarly journals Metabolism Control in 3D Printed Living Materials

2021 ◽  
Author(s):  
Tobias Butelmann ◽  
Hans Priks ◽  
Zoel Parent ◽  
Trevor G. Johnston ◽  
Tarmo Tamm ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Terminal Electron Acceptor ◽  
Three Dimensional Printing ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Brewing Process ◽  
3D Printed ◽  
Dimensional Printing ◽  
Living Materials

AbstractThe three-dimensional printing of cells offers an attractive opportunity to design and develop innovative biotechnological applications, such as the fabrication of biosensors or modular bioreactors. Living materials (LMs) are cross-linked polymeric hydrogel matrices containing cells, and recently, one of the most deployed LMs consists of F127-bis-urethane methacrylate (F127-BUM). The material properties of F127-BUM allow reproducible 3D printing and stability of LMs in physiological environments. These materials are permissible for small molecules like glucose and ethanol. However, no information is available for oxygen, which is essential— for example, towards the development of aerobic bioprocesses using microbial cell factories. To address this challenge, we investigated the role of oxygen as a terminal electron acceptor in the budding yeast’s respiratory chain and determined its permissibility in LMs. We quantified the ability of cell-retaining LMs to utilize oxygen and compared it with cells in suspension culture. We found that the cells’ ability to consume oxygen was heavily impaired inside LMs, indicating that the metabolism mostly relied on fermentation instead of respiration. To demonstrate an application of these 3D printed LMs, we evaluated a comparative brewing process. The analysis showed a significantly higher (3.7%) ethanol production using 3D printed LMs than traditional brewing, indicating an efficient control of the metabolism. Towards molecular and systems biology studies using LMs, we developed a highly reliable method to isolate cells from LMs for flow cytometry and further purified macromolecules (proteins, RNA, and DNA). Our results show the application of F127-BUM-based LMs for microaerobic processes and envision the development of diverse bioprocesses using versatile LMs in the future.

Central ossifying fibroma of mandible

2020 ◽  
Vol 13 (12) ◽  
pp. e239286
Author(s):  
Kumar Nilesh ◽  
Prashant Punde ◽  
Nitin Shivajirao Patil ◽  
Amol Gautam
Keyword(s):  
Fibrous Tissue ◽  
Three Dimensional ◽  
Facial Asymmetry ◽  
Ossifying Fibroma ◽  
Mandible Reconstruction ◽  
Three Dimensional Printing ◽  
Osseous Lesion ◽  
Large Size ◽  
Dimensional Printing

Ossifying fibroma (OF) is a rare, benign, fibro-osseous lesion of the jawbone characterised by replacement of the normal bone with fibrous tissue. The fibrous tissue shows varying amount of calcified structures resembling bone and/or cementum. The central variant of OF is rare, and shows predilection for mandible among the jawbone. Although it is classified as fibro-osseous lesion, it clinically behaves as a benign tumour and can grow to large size, causing bony swelling and facial asymmetry. This paper reports a case of large central OF of mandible in a 40-year-old male patient. The lesion was treated by segmental resection of mandible. Reconstruction of the surgical defect was done using avascular fibula bone graft. Role of three-dimensional printing of jaw and its benefits in surgical planning and reconstruction are also highlighted.

scholarly journals Influence of the Infill Geometry of 3D-Printed Tablets on Drug Dissolution

2021 ◽  
Vol 5 (1) ◽  
pp. 15
Author(s):  
Nuno Venâncio ◽  
Gabriela G. Pereira ◽  
João F. Pinto ◽  
Ana I. Fernandes
Keyword(s):  
Three Dimensional ◽  
Drug Dissolution ◽  
Fused Deposition Modelling ◽  
Three Dimensional Printing ◽  
Preliminary Results ◽  
Fused Deposition ◽  
3D Printed ◽  
Dimensional Printing ◽  
Hot Melt ◽  
Patient Centric

Patient-centric therapy is especially important in pediatrics and may be attained by three-dimensional printing. Filaments containing 30% w/w of theophylline were produced by hot-melt extrusion and printed using fused deposition modelling to produce tablets. Here, preliminary results evaluating the effect of infill geometry (cross, star, grid) on drug content and release are reported.

3D-Printed Conductive Filaments Based on Carbon Nanostructures Embedded in a Polymer Matrix

2021 ◽  
pp. 1725-1742
Author(s):  
Diogo José Horst ◽  
Pedro Paulo Andrade Junior
Keyword(s):  
Polymer Matrix ◽  
Carbon Nanostructures ◽  
Three Dimensional ◽  
Full Potential ◽  
Electrically Conductive ◽  
Three Dimensional Printing ◽  
Current State ◽  
3D Printed ◽  
The Subject ◽  
Dimensional Printing

Conductive and magnetic filaments are revolutionizing three-dimensional printing (3DP) to a new level. This review study presents the current state of the art on the subject, summarizing recent high impact studies about main advances regarding the application of 3DP filaments based on carbon nanostructures such as graphene, carbon fibers, nanotubes, and conductive carbon black embedded in a polymer matrix, by reviewing its main characteristics and showing the main producers and also the products available on the market. The availability of inexpensive, reliable, and electrically conductive material will be indispensable for the fabrication of circuits and sensors before the full potential of 3DP for customized products incorporating electrical elements can be fully explored.

scholarly journals 3D-Printed Soft Structure of Polyurethane and Magnetorheological Fluid: A Proof-of-Concept Investigation of its Stiffness Tunability

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 655 ◽  
Author(s):  
Seong-Woo Hong ◽  
Ji-Young Yoon ◽  
Seong-Hwan Kim ◽  
Sun-Kon Lee ◽  
Yong-Rae Kim ◽  
...  
Keyword(s):  
Fused Deposition Modeling ◽  
Permanent Magnets ◽  
Thermoplastic Polyurethane ◽  
Three Dimensional ◽  
Magnetorheological Fluid ◽  
Three Dimensional Printing ◽  
Fused Deposition ◽  
Deposition Modeling ◽  
3D Printed ◽  
Dimensional Printing

In this study, a soft structure with its stiffness tunable by an external field is proposed. The proposed soft beam structure consists of a skin structure with channels filled with a magnetorheological fluid (MRF). Two specimens of the soft structure are fabricated by three-dimensional printing and fused deposition modeling. In the fabrication, a nozzle is used to obtain channels in the skin of the thermoplastic polyurethane, while another nozzle is used to fill MRF in the channels. The specimens are tested by using a universal tensile machine to evaluate the relationships between the load and deflection under two different conditions, without and with permanent magnets. It is empirically shown that the stiffness of the proposed soft structure can be altered by activating the magnetic field.

Three-Dimensional Printing of Prenatal Ultrasonographic Diagnosis of Cleft Lip and Palate: Presenting the Needed “Know-How” and Discussing Its Use in Parental Education

2020 ◽  
Vol 57 (8) ◽  
pp. 1041-1044
Author(s):  
Matthias Schlund ◽  
Jean-Marc Levaillant ◽  
Romain Nicot
Keyword(s):  
Parental Education ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Three Dimensional ◽  
3D Models ◽  
Prenatal Counseling ◽  
Three Dimensional Printing ◽  
3D Printed ◽  
3D Information ◽  
Dimensional Printing

Parental prenatal counseling is of paramount significance since parents often experience an emotional crisis with feelings of disappointment and helplessness. Three-dimensional (3D) printed model of the unborn child’s face presenting with cleft lip and palate, based on ultrasonographic information, could be used to provide visual 3D information, further enhancing the prospective parent’s comprehension of their unborn child’s pathology and morphology, helping them to be psychologically prepared and improving the communication with the caretaking team. Prospective parents appreciate if prenatal counseling is available with the most detailed information as well as additional resources. The technique necessary to create 3D models after ultrasonographic information is explained, and the related costs are evaluated. The use of such models in parental education is then discussed.

The role of three‐dimensional printing in the surgical management of breast cancer

2019 ◽  
Vol 120 (6) ◽  
pp. 897-902 ◽  
Author(s):  
Lumarie Santiago ◽  
Beatriz E. Adrada ◽  
Abigail S. Caudle ◽  
Mark W. Clemens ◽  
Dalliah M. Black ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Surgical Management ◽  
Three Dimensional ◽  
Three Dimensional Printing ◽  
Dimensional Printing
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