scholarly journals Assessment of the suitability of marker fragments DNA sequences of the internal transcribed spacer region (ITS), beta-tubulin gene (TUB) and elongation factor (EF1) genes for diagnostics of Fusarium tricinctum species complex in Russian in grain products

2021 ◽  
Author(s):  
A. Shchukovskaya
Keyword(s):  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Species Complex ◽  
Elongation Factor ◽  
Internal Transcribed Spacer Region ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Interspecific Variability ◽  
Fusarium Tricinctum ◽  
Beta Tubulin Gene

AbstractBy results of laboratory researches from plant samples of grain crops species Fusarium avenaceum, Fusarium tricinctum and Fusarium acuminatum related to Fusarium tricinctum species complex were isolated and identified, based on cultural and morphological characteristics, molecular-genetic analysis of 3 DNA fragments was performed: internal transcribed spacer (ITS) site, beta-tubulin gene site (TUB), elongation factor gene site (EF1) showed insignificant intraspecific and interspecific variability at ITS and EF1 sites, which may negatively affect the reliability of target species identification, TUB site was more effective for distinguishing and identifying the studied species, due to significant intraspecific and interspecific variability

scholarly journals Potential possibility of using the DNA regions of the internal transcribed spacer ITS and the beta-tubulin gene TUB in the diagnosis of the pathogen Mycosphaerella linicola Naumov

2021 ◽  
Author(s):  
A. Shchukovskaya ◽  
O. Skripka ◽  
D. Uvarova ◽  
A. Shipulin
Keyword(s):  
Internal Transcribed Spacer ◽  
Population Level ◽  
Target Species ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Potential Possibility ◽  
Genetic Level ◽  
Geographic Regions ◽  
Similarities And Differences ◽  
Beta Tubulin Gene

AbstractDuring the study, the suitability of the ITS and TUB DNA regions was shown for identifying the target species at the genetic level. However, these DNA regions do not reveal significant differences between the sequences of the target species from different geographic regions. However, when constructing dendrograms of similarities and differences, the sequential differentiation between isolates at the population level was found, which may further affect the results of identification of the target species.

scholarly journals Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Mahboobeh Kharazi ◽  
Bahram Ahmadi ◽  
Koichi Makimur ◽  
Armin Farhang ◽  
Sahar Kianipour ◽  
...  
Keyword(s):  
Phylogenetic Tree ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Candida Parapsilosis ◽  
Sequence Difference ◽  
Species Variation ◽  
Its Sequencing ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Beta Tubulin Gene

Background and Purpose: Candida parapsilosis is a common cause of candidemia in children and patients with onco-hematological diseases, septic arthritis, peritonitis, vaginitis, and nail and skin infections. Regarding this, the present study was condcuted to evaluate intra- and inter-species variation within beta-tubulin DNA sequence of C. parapsilosis complex in order to establish the utilization of this gene in the identification and phylogenetic analysis of the species. Materials and Methods: A total of 23 isolates representing three different species of C. parapsilosis complex were used in this study, all of which were identifed by ITS-sequencing. For the successful amplification of beta-tubulin gene, a newly designed set of pan-Candida primers was used, followed by bilaterally sequenc analysis for pairwise comparisons, determination of multiple alignments, evaluation of sequence identity levels, counting sequence difference, and construction of phylogenetic tree. Results: The multiple alignment of 623-629 bp-long nucleotide (nt) sequences reflecting the beta-tubulin gene indicated an inter-species divergence ranging within 0-68 nt in C. parapsilosis, C. orthopsilosis, and C. metapsilosis with a mean similarity of 84.7% among the species. Meanwhile, the intra-species differences of 0-20 and 0-6 nt were found between the strains of C. parapsilosis and C. orthopsilosis, respectively. The phylogenetic tree topology was characterized by a clade made up by C. parapsilosis and C. orthopsilosis, while C. metapsilosis formed a related but separate lineage. Conclusion: Our data provided the basis for further discoveries of the relationship between the species belonging to C. parapsilosis complex. Furthermore, the findigns of the prsent study revealed the efficiency of beta-tubulin DNA sequence data in the identification and taxonomy of C. parapsilosis and other pathogenic yeasts.

Phylogenetic relationships ofCylindrocladium pseudogracileandCylindrocladium rumohraewith morphologically similar taxa, based on morphology and DNA sequences of internal transcribed spacers and beta-tubulin

2000 ◽  
Vol 77 (12) ◽  
pp. 1813-1820
Author(s):  
Pedro W Crous ◽  
Ji-Chuan Kang ◽  
Conrad L Schoch ◽  
Godwin RA Mchau
Keyword(s):  
The Netherlands ◽  
Dna Sequences ◽  
Phylogenetic Trees ◽  
Internal Transcribed Spacers ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Amazonas State ◽  
Reference Strains ◽  
Its1 And Its2 ◽  
Beta Tubulin Gene

Unidentified strains of Cylindrocladium that were baited from soil in the Amazonas state of Brazil or isolated from Adiantum in the Netherlands were examined morphologically and analysed phylogenetically in comparison with reference strains. Phylogenetic trees inferred from the 5.8S subunit and flanking internal transcribed spacers (ITS1 and ITS2) of rDNA, as well as the beta-tubulin gene, separated species in accordance with their morphological features and characteristics. Although species differences based on ITS sequences were consistent, there were only a few informative sites available, making it difficult to clearly identify the unknown strains. Better resolution in separation of species was achieved from the beta-tubulin data. The strains baited from soil in Brazil were found to represent two species, namely Cylindrocladium gracile (Bugnic.) Boesew. and Cylindrocladium pseudogracile Crous. Morphologically, these two species are similar, except that the latter has slightly narrower, 1(-3)-septate conidia, and produces a Calonectria teleomorph. Furthermore, the strain from the Netherlands represents Cylindrocladium rumohrae El-Gholl & Alfenas, a species previously known only from Panama. Results of this study also indicated that the beta-tubulin gene is phylogenetically more informative than the ITS regions for distinguishing species of Cylindrocladium.

scholarly journals Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Mahboobeh Kharazi ◽  
Bahram Ahmadi ◽  
Koichi Makimur ◽  
Armin Farhang ◽  
Sahar Kianipour ◽  
...  
Keyword(s):  
Phylogenetic Tree ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Candida Parapsilosis ◽  
Sequence Difference ◽  
Species Variation ◽  
Its Sequencing ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Beta Tubulin Gene

Background and Purpose: Candida parapsilosis is a common cause of candidemia in children and patients with onco-hematological diseases, septic arthritis, peritonitis, vaginitis, and nail and skin infections. Regarding this, the present study was condcuted to evaluate intra- and inter-species variation within beta-tubulin DNA sequence of C. parapsilosis complex in order to establish the utilization of this gene in the identification and phylogenetic analysis of the species. Materials and Methods: A total of 23 isolates representing three different species of C. parapsilosis complex were used in this study, all of which were identifed by ITS-sequencing. For the successful amplification of beta-tubulin gene, a newly designed set of pan-Candida primers was used, followed by bilaterally sequenc analysis for pairwise comparisons, determination of multiple alignments, evaluation of sequence identity levels, counting sequence difference, and construction of phylogenetic tree. Results: The multiple alignment of 623-629 bp-long nucleotide (nt) sequences reflecting the beta-tubulin gene indicated an inter-species divergence ranging within 0-68 nt in C. parapsilosis, C. orthopsilosis, and C. metapsilosis with a mean similarity of 84.7% among the species. Meanwhile, the intra-species differences of 0-20 and 0-6 nt were found between the strains of C. parapsilosis and C. orthopsilosis, respectively. The phylogenetic tree topology was characterized by a clade made up by C. parapsilosis and C. orthopsilosis, while C. metapsilosis formed a related but separate lineage. Conclusion: Our data provided the basis for further discoveries of the relationship between the species belonging to C. parapsilosis complex. Furthermore, the findigns of the prsent study revealed the efficiency of beta-tubulin DNA sequence data in the identification and taxonomy of C. parapsilosis and other pathogenic yeasts.

scholarly journals Nucleotide sequence analysis of beta tubulin gene in a wide range of dermatophytes

2014 ◽  
Vol 52 (7) ◽  
pp. 674-688 ◽  
Author(s):  
Ali Rezaei-Matehkolaei ◽  
Hossein Mirhendi ◽  
Koichi Makimura ◽  
G. Sybren de Hoog ◽  
Kazuo Satoh ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Nucleotide Sequence Analysis ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Wide Range ◽  
Beta Tubulin Gene

The beta tubulin gene of Eimeria tenella

1996 ◽  
Vol 76 (1-2) ◽  
pp. 315-319 ◽  
Author(s):  
Guan Zhu ◽  
Janet S. Keithly
Keyword(s):  
Eimeria Tenella ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Beta Tubulin Gene

Mutational analysis of the class i beta-tubulin gene in renal cell carcinoma (RCC)

2003 ◽  
Vol 2 (6) ◽  
pp. 66
Author(s):  
Roisean E Ferguson ◽  
Claire F Taylor ◽  
Anthea J Stanley ◽  
Roger M Phillips ◽  
Adrian D Joyce ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Mutational Analysis ◽  
Class I ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Beta Tubulin Gene
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