Cinnamoyl-CoA Reductase 1 (CCR1) and CCR2 Function Divergently in Tissue Lignification, Flux Control and Cross-talk with Glucosinolate Pathway in Brassica napus

Cinnamoyl-CoA reductase (CCR) is the entry point of lignin pathway and a crucial locus in dissection and manipulation of associated traits. Brassica crops have worldwide importance, but their CCR-related metabolisms and traits are largely uncharacterized. Here, 16 CCR genes are identified from B. napus and its parental species B. rapa and B. oleracea. They are divided into CCR1 subfamily and CCR2 subfamily, which differ from each other in organ-specificity, participation in yellow-seed trait and responses to various stresses. BnCCR1 is preferentially involved in biosynthesis of G and H lignins and development of vascular system, while BnCCR2 is preferentially involved in biosynthesis of S lignin and development of interfascicular fibers. BnCCR1 has stronger effects on lignification-related development, lodging resistance, flux control and seed color, whereas BnCCR2 has stronger effect on sinapates biosynthesis. BnCCR1 overexpressing plants show a delay in bolting and flowering, while BnCCR2 overexpressing plants have less developed vascular system in leaf due to suppressed G lignin accumulation. Unexpectedly, both BnCCR1 and BnCCR2 overexpressors show no improvement in resistance to UV-B and S. sclerotiorum. Besides, their glucosinolate profiles are greatly and almost oppositely remodeled through pathway crosstalk. These results provide systemic dissection on Brassica CCRs and CCR1-CCR2 divergence in Brassicaceae.

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Two types of cinnamoyl-CoA reductase function divergently in accumulation of lignins, flavonoids and glucosinolates and enhance lodging resistance in Brassica napus

The Crop Journal ◽

10.1016/j.cj.2021.10.002 ◽

2021 ◽

Author(s):

Nengwen Yin ◽

Bo Li ◽

Xue Liu ◽

Ying Liang ◽

Jianping Lian ◽

...

Keyword(s):

Brassica Napus ◽

Lodging Resistance ◽

Cinnamoyl Coa Reductase ◽

Coa Reductase

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The OsFBK1 E3 Ligase Subunit Affects Anther and Root Secondary Cell Wall Thickenings by Mediating Turnover of a Cinnamoyl-CoA Reductase

PLANT PHYSIOLOGY ◽

10.1104/pp.17.01733 ◽

2018 ◽

Vol 176 (3) ◽

pp. 2148-2165 ◽

Cited By ~ 9

Author(s):

Pratikshya Borah ◽

Jitendra P. Khurana

Keyword(s):

Cell Wall ◽

Secondary Cell Wall ◽

E3 Ligase ◽

Cinnamoyl Coa Reductase ◽

Coa Reductase

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Mapping and identification of yellow seed coat color genes in Brassica juncea L.

10.21203/rs.3.rs-25889/v1 ◽

2020 ◽

Author(s):

Zhen Huang ◽

Yang Wang ◽

Hong Lu ◽

Xiang Liu ◽

Lu Liu ◽

...

Keyword(s):

Candidate Gene ◽

Brassica Juncea ◽

Seed Coat ◽

Coat Color ◽

Seed Color ◽

Seed Coat Color ◽

Flavonoid Pathway ◽

Yellow Seed ◽

Color Formation ◽

Yellow Seed Coat

Abstract BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

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