scholarly journals Transcriptional activation of rice CINNAMOYL‐CoA REDUCTASE 10 by OsNAC5, contributes to drought tolerance by modulating lignin accumulation in roots

2021 ◽  
Author(s):  
Seung Woon Bang ◽  
Seowon Choi ◽  
Xuanjun Jin ◽  
Se Eun Jung ◽  
Joon Weon Choi ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transcriptional Activation ◽  
Cinnamoyl Coa Reductase ◽  
Lignin Accumulation ◽  
Coa Reductase

scholarly journals The OsFBK1 E3 Ligase Subunit Affects Anther and Root Secondary Cell Wall Thickenings by Mediating Turnover of a Cinnamoyl-CoA Reductase

2018 ◽  
Vol 176 (3) ◽  
pp. 2148-2165 ◽  
Author(s):  
Pratikshya Borah ◽  
Jitendra P. Khurana
Keyword(s):  
Cell Wall ◽  
Secondary Cell Wall ◽  
E3 Ligase ◽  
Cinnamoyl Coa Reductase ◽  
Coa Reductase

Treatment of mammalian cells with the endoplasmic reticulum-proliferator compactin strongly induces recombinant and endogenous xenobiotic metabolizing enzymes and 3-hydroxy-3-methylglutaryl-CoA reductase in vitro

1999 ◽  
Vol 112 (4) ◽  
pp. 515-523
Author(s):  
L. McLaughlin ◽  
B. Burchell ◽  
M. Pritchard ◽  
C.R. Wolf ◽  
T. Friedberg
Keyword(s):  
Endoplasmic Reticulum ◽  
Enzyme Activity ◽  
Transcriptional Activation ◽  
Mammalian Cells ◽  
Reductase Activity ◽  
Cholesterol Biosynthesis ◽  
Metabolizing Enzymes ◽  
P450 Reductase ◽  
Coa Reductase

Some xenobiotics induce membrane-bound drug metabolizing enzymes (Xme) and a profound proliferation of the endoplasmic reticulum (ER) in vivo. However these effects are much weaker in vitro, possibly due to absence of certain transcription factors. We tested the possibility that ER proliferation can affect the level of ER-resident enzymes even in the absence of transcriptional activation. For this purpose we analysed the effects of compactin, which has been shown to induce ER proliferation in vitro, on recombinant Xme, which were expressed from a constitutive viral promoter. High levels of recombinant UDP-glucuronosyltransferase UGT1A6 were achieved by amplification of the UGT1A6 cDNA using the dihydrofolate reductase cDNA as selectable marker in DHFR- CHO cells. Treatment of the resulting cell lines with lipoprotein-deficient serum in the absence and presence of compactin for 5 days resulted in a 1.3- and 2.3-fold, respectively, increase of the UGT enzyme activity towards 4-methylumbelliferone, paralleled by an induction of immunoreactive UGT1A6 protein. Similarly, treatment with this 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor increased the endogenous P450 reductase activity 2.6-fold, concomitant with an increase of immunodetectable protein. As expected compactin induced the level of 3-hydroxy-3-methylglutaryl-CoA reductase. Increased levels of this protein have been associated with a proliferation of the ER. Compactin treatment of a separate cell line that expressed recombinant human P450 reductase increased this enzyme activity fivefold. Pulse-chase experiments revealed that the induction of the recombinant Xme by compactin was most likely due to decreased protein degradation. Our results show that enzyme systems unrelated to those involved in cholesterol biosynthesis are affected by compounds known to affect membrane biogenesis. Since this effect extends to heterologously expressed enzymes, it also provides an efficient means by which to increase the levels of recombinant ER proteins.

The transcription factor ZmNAC49 reduces stomatal density and improves drought tolerance in maize

2020 ◽  
Author(s):  
Yang Xiang ◽  
Xiujuan Sun ◽  
Xiangli Bian ◽  
Tianhui Wei ◽  
Tong Han ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Stomatal Conductance ◽  
Drought Tolerance ◽  
Transpiration Rate ◽  
Transcriptional Activation ◽  
Stomatal Density ◽  
Stomatal Development ◽  
Expression Of Genes ◽  
Growth Development

Abstract Drought stress severely limits the growth, development, and productivity of crops, and therefore understanding the mechanisms by which plants respond to drought is crucial. In this study, we cloned a maize NAC transcription factor, ZmNAC49, and identified its function in response to drought stress. We found that ZmNAC49 is localized in the nucleus and has transcriptional activation activity. ZmNAC49 expression is rapidly and strongly induced by drought stress, and overexpression enhances stress tolerance in maize. Overexpression also significant decreases the transpiration rate, stomatal conductance, and stomatal density in maize. Detailed study showed that ZmNAC49 overexpression affects the expression of genes related to stomatal development, namely ZmTMM, ZmSDD1, ZmMUTE, and ZmFAMA. In addition, we found that ZmNAC49 can directly bind to the promoter of ZmMUTE and suppress its expression. Taken together, our results show that the transcription factor ZmNAC49 represses ZmMUTE expression, reduces stomatal density, and thereby enhances drought tolerance in maize.

scholarly journals Structural Studies of Cinnamoyl-CoA Reductase and Cinnamyl-Alcohol Dehydrogenase, Key Enzymes of Monolignol Biosynthesis

2014 ◽  
Vol 26 (9) ◽  
pp. 3709-3727 ◽  
Author(s):  
H. Pan ◽  
R. Zhou ◽  
G. V. Louie ◽  
J. K. Muhlemann ◽  
E. K. Bomati ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Structural Studies ◽  
Cinnamyl Alcohol ◽  
Key Enzymes ◽  
Cinnamoyl Coa Reductase ◽  
Monolignol Biosynthesis ◽  
Coa Reductase

scholarly journals Reduced lignin content and altered lignin composition in the warm season forage grass Paspalum dilatatum by down-regulation of a Cinnamoyl CoA Reductase Gene

2014 ◽  
Vol 23 (3) ◽  
pp. 503-517 ◽  
Author(s):  
Andrea Giordano ◽  
Zhiqian Liu ◽  
Stephen N. Panter ◽  
Adam M. Dimech ◽  
Yongjin Shang ◽  
...  
Keyword(s):  
Lignin Content ◽  
Warm Season ◽  
Forage Grass ◽  
Paspalum Dilatatum ◽  
Down Regulation ◽  
Cinnamoyl Coa Reductase ◽  
Reductase Gene ◽  
Lignin Composition ◽  
Coa Reductase

Identification of the structure and origin of a thioacidolysis marker compound for ferulic acid incorporation into angiosperm lignins (and an indicator for cinnamoyl CoA reductase deficiency)

2007 ◽  
Vol 53 (2) ◽  
pp. 368-379 ◽  
Author(s):  
John Ralph ◽  
Hoon Kim ◽  
Fachuang Lu ◽  
John H. Grabber ◽  
Jean-Charles Leplé ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Reductase Deficiency ◽  
Marker Compound ◽  
Cinnamoyl Coa Reductase ◽  
Acid Incorporation ◽  
Coa Reductase

scholarly journals Loss of transcriptional activation of three sterol-regulated genes in mutant hamster cells.

1993 ◽  
Vol 13 (9) ◽  
pp. 5175-5185 ◽  
Author(s):  
M J Evans ◽  
J E Metherall
Keyword(s):  
Transcriptional Regulation ◽  
Cell Lines ◽  
Transcriptional Activation ◽  
Cho Cells ◽  
Mutant Cell ◽  
Chinese Hamster ◽  
Wild Type ◽  
Hmg Coa Reductase ◽  
Low Levels ◽  
Coa Reductase

Cholesterol biosynthesis and uptake are controlled by a classic end product-feedback mechanism whereby elevated cellular sterol levels suppress transcription of the genes encoding 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, HMG-CoA reductase, and the low-density lipoprotein receptor. The 5'-flanking region of each gene contains a common cis-acting element, designated the sterol regulatory element (SRE), that is required for transcriptional regulation. In this report, we describe mutant Chinese hamster ovary (CHO) cell lines that lack SRE-dependent transcription. Mutant cell lines were isolated on the basis of their ability to survive treatment with amphotericin B, a polyene antibiotic that kills cells by interacting with cholesterol in the plasma membrane. Four mutant lines (SRD-6A, -B, -C, and -D) were found to be cholesterol auxotrophs and demonstrated constitutively low levels of mRNA for all three sterol-regulated genes even under conditions of sterol deprivation. The mutant cell lines were found to be genetically recessive, and all four lines belonged to the same complementation group. When transfected with a plasmid containing a sterol-regulated promoter fused to a bacterial reporter gene, SRD-6B cells demonstrated constitutively low levels of transcription, in contrast to wild-type CHO cells, which increased transcription under conditions of sterol deprivation. Mutation of the SREs in this plasmid prior to transfection reduced the level of expression in wild-type CHO cells deprived of sterols to the level of expression found in SRD-6B cells. The defect in SRD-6 cells is limited to transcriptional regulation, since posttranscriptional mechanisms of sterol-mediated regulation were intact: the cells retained the ability to posttranscriptionally suppress HMG-CoA reductase activity and to stimulate acyl-CoA:cholesterol acyltransferase activity. These results suggest that SRD-6 cells lack a factor required for SRE-dependent transcriptional activation. We contrast these cells with a previously isolated oxysterol-resistant cell line (SRD-2) that lacks a factor required for SRE-dependent transcriptional suppression and propose a model for the role of these genetically defined factors in sterol-mediated transcriptional regulation.

Expression analysis of cinnamoyl-CoA reductase (CCR) gene in developing seedlings of Leucaena leucocephala: A pulp yielding tree species

2011 ◽  
Vol 49 (2) ◽  
pp. 138-145 ◽  
Author(s):  
Sameer Srivastava ◽  
Ranadheer K. Gupta ◽  
Manish Arha ◽  
Rishi K. Vishwakarma ◽  
Shuban K. Rawal ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Tree Species ◽  
Leucaena Leucocephala ◽  
Cinnamoyl Coa Reductase ◽  
Coa Reductase
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