scholarly journals The F-box protein AFF1 regulates ARF protein accumulation to regulate auxin response

2021 ◽  
Author(s):  
Hongwei Jing ◽  
David Korasick ◽  
Ryan Emenecker ◽  
Nicholas Morffy ◽  
Edward Wilkinson ◽  
...  
Keyword(s):  
Protein Stability ◽  
Molecular Mechanisms ◽  
Functional Characterization ◽  
26S Proteasome ◽  
Auxin Response Factor ◽  
Protein Accumulation ◽  
Response Factor ◽  
Auxin Response ◽  
Transcriptional Responses ◽  
Arf Protein

Auxin critically regulates nearly every aspect of plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. Although ARF protein stability is regulated via the 26S proteasome, molecular mechanisms underlying ARF stability and turnover are unknown. Here, we report the identification and functional characterization of an F-box E3 ubiquitin ligase, which we have named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). AFF1 directly interacts with ARF19 and regulates its accumulation. Mutants defective in AFF1 display ARF19 protein hyperaccumulation, attenuated auxin responsiveness, and developmental defects. Together, our data suggest a new mechanism, namely control of ARF protein stability, in regulating auxin response.

scholarly journals Auxin response factor gene family in Brassica rapa: genomic organization, divergence, expression, and evolution

2012 ◽  
Vol 287 (10) ◽  
pp. 765-784 ◽  
Author(s):  
Jeong-Hwan Mun ◽  
Hee-Ju Yu ◽  
Ja Young Shin ◽  
Mijin Oh ◽  
Hyun-Ju Hwang ◽  
...  
Keyword(s):  
Gene Family ◽  
Brassica Rapa ◽  
Genomic Organization ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Auxin Response ◽  
Factor Gene ◽  
Auxin Response Factor Gene

Genome-wide analysis of the auxin response factor (ARF) gene family in maize (Zea mays)

2010 ◽  
Vol 63 (3) ◽  
pp. 225-234 ◽  
Author(s):  
Yan Liu ◽  
HaiYang Jiang ◽  
Wenjuan Chen ◽  
Yexiong Qian ◽  
Qing Ma ◽  
...  
Keyword(s):  
Zea Mays ◽  
Gene Family ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Auxin Response ◽  
Genome Wide Analysis ◽  
Genome Wide

scholarly journals Genome-wide identification and expression profiling of the auxin response factor (ARF) gene family in physic nut

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0201024 ◽  
Author(s):  
Yuehui Tang ◽  
Xinxin Bao ◽  
Kun Liu ◽  
Jian Wang ◽  
Ju Zhang ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Profiling ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Physic Nut ◽  
Auxin Response ◽  
Genome Wide

scholarly journals Identification of Auxin Response Factor-Encoding Genes Expressed in Distinct Phases of Leaf Vein Development and with Overlapping Functions in Leaf Formation

Plants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 242
Author(s):  
Mathias Schuetz ◽  
Mario Fidanza ◽  
Jim Mattsson
Keyword(s):  
Expression Patterns ◽  
Auxin Response Factor ◽  
Response Factor ◽  
Auxin Response ◽  
Leaf Vein ◽  
Vessel Elements ◽  
Leaf Formation ◽  
Combined Action ◽  
Mutant Phenotypes

Based on mutant phenotypes the MONOPTEROS (MP)/Auxin Response Factor 5 (ARF5) gene acts in several developmental processes including leaf vein development. Since overlapping functions among ARF genes are common, we assessed the related ARF 3-8 and 19 genes for potential overlap in expression during vein development using in-situ hybridization. Like MP/ARF5, ARF3 was expressed in preprocambial and procambial cells. ARF7 was also expressed in procambial cells, close to and during vein differentiation. ARF19 was expressed in differentiating vessel elements. To assess if genes with vein expression have overlapping functions, double mutants were generated. While arf3, 5 and 7 mutants formed leaves normally, double mutant combinations of mp/arf5 with arf3 or arf7 resulted in a breakdown of leaf formation. Instead, novel structures not present in any of the single mutants formed. The results implicate ARF3 and ARF7 in rosette leaf formation and suggest that their functions overlap and act in parallel with MP/ARF5 in this process. The observed vascular expression patterns suggest unique functions (ARF7 and 19) and potentially overlapping functions (ARF3 and 5) in vein development. Since arf3 arf5 double mutants do not form leaves, assessment of their potential combined action in vein development will require the use of conditional mutants.

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