Evidence of MHC class I and II influencing viral and helminth infection via the microbiome in a non-human primate

Until recently, the study of major histocompability complex (MHC) mediated immunity has focused on the direct link between MHC variability and susceptibility to parasite infection. However, MHC genes can also influence host health indirectly through the sculpting of the bacterial community that in turn shape immune responses. We investigated the links between MHC class I and II gene variability gut microbiome diversity and micro- (adenovirus, AdV) and macro- (helminth) parasite infection probabilities in a wild population of non-human primates, mouse lemurs of Madagascar. This setup encompasses a plethora of underlying interactions between parasites, microbes and adaptive immunity in natural populations. Both MHC classes explained shifts in microbiome composition and the effect was driven by a few select microbial taxa. Among them were three taxa ( Odoribacter , Campylobacter and Prevotellaceae-UCG-001) which were in turn linked to AdV and helminth infection status, evidence of the indirect effect of the MHC via the microbiome. Our study provides support for the coupled role of MHC variability and microbial flora as contributing factors of parasite infection.

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Evidence of MHC class I and II influencing viral and helminth infection via the microbiome in a non-human primate

PLoS Pathogens ◽

10.1371/journal.ppat.1009675 ◽

2021 ◽

Vol 17 (11) ◽

pp. e1009675

Author(s):

B. Karina Montero ◽

Wasim Uddin ◽

Nina Schwensow ◽

Mark A. F. Gillingham ◽

Yedidya R. Ratovonamana ◽

...

Keyword(s):

Mhc Class I ◽

Helminth Infection ◽

Gene Diversity ◽

Natural Populations ◽

Parasite Infection ◽

Class I ◽

Contributing Factors ◽

Microbiome Composition ◽

Mhc Genes ◽

Mhc Diversity

Until recently, the study of major histocompability complex (MHC) mediated immunity has focused on the direct link between MHC diversity and susceptibility to parasite infection. However, MHC genes can also influence host health indirectly through the sculpting of the bacterial community that in turn shape immune responses. We investigated the links between MHC class I and II gene diversity gut microbiome diversity and micro- (adenovirus, AdV) and macro- (helminth) parasite infection probabilities in a wild population of non-human primates, mouse lemurs of Madagascar. This setup encompasses a plethora of underlying interactions between parasites, microbes and adaptive immunity in natural populations. Both MHC classes explained shifts in microbiome composition and the effect was driven by a few select microbial taxa. Among them were three taxa (Odoribacter, Campylobacter and Prevotellaceae-UCG-001) which were in turn linked to AdV and helminth infection status, correlative evidence of the indirect effect of the MHC via the microbiome. Our study provides support for the coupled role of MHC diversity and microbial flora as contributing factors of parasite infection.

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Specific MHC class I supertype associated with parasite infection and color morph in a wild lizard population

Ecology and Evolution ◽

10.1002/ece3.4479 ◽

2018 ◽

Vol 8 (19) ◽

pp. 9920-9933 ◽

Cited By ~ 3

Author(s):

Jessica D. Hacking ◽

Devi Stuart-Fox ◽

Stephanie S. Godfrey ◽

Michael G. Gardner

Keyword(s):

Mhc Class I ◽

Parasite Infection ◽

Class I ◽

Color Morph

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Diversity and selection of MHC class I genes in the Godlewski's bunting

10.22541/au.160796185.57504253/v1 ◽

2020 ◽

Author(s):

Wei Huang ◽

Boye Liu ◽

Tobias Lenz ◽

Yangyang Peng ◽

Yan-Yun Zhang ◽

...

Keyword(s):

Amino Acid ◽

Mhc Class I ◽

Biological Diversity ◽

Balancing Selection ◽

Class I ◽

Immune Gene ◽

Amino Acid Polymorphism ◽

Mountain Area ◽

Mhc Genes ◽

High Amino Acid

The major histocompatibility complex (MHC) is a multiple-copy immune gene family in vertebrates. Its genes are highly variable and code for antigen-presenting molecules. Characterization of MHC genes in different species and investigating the mechanisms that shape MHC diversity is an important goal in understanding the evolution of biological diversity. Here we developed a next generation sequencing (NGS) protocol to genotype the MHC class I genes of 326 Godlewski’s buntings (Emberiza godlewskii) sampled in the Western mountain area of Beijing from 2014 to 2016. A total of 184 functional alleles were identified, including both non-classical and classical alleles. Classical alleles could be clustered into nine supertypes. Compared with other passerine birds, the individual diversity of MHC class I genes in Godlewski’s buntings is intermediate. Ten amino acid sites in the antigen-binding domain showed signatures of positive selection and eight of them exhibit high amino acid polymorphism. These findings indicate the action of balancing selection and provide a framework for subsequent investigation of selection acting on MHC genes in Godlewski’s buntings.

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Allelic diversity and patterns of selection at the major histocompatibility complex class I and II loci in a threatened shorebird, the Snowy Plover (Charadrius nivosus)

BMC Evolutionary Biology ◽

10.1186/s12862-020-01676-7 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Medardo Cruz-López ◽

Guillermo Fernández ◽

Helen Hipperson ◽

Eduardo Palacios ◽

John Cavitt ◽

...

Keyword(s):

Mhc Class I ◽

Mhc Class Ii ◽

Balancing Selection ◽

Allelic Diversity ◽

Class Ii ◽

Class I ◽

Mhc Genes ◽

Snowy Plover ◽

Snowy Plovers ◽

Private Alleles

Abstract Background Understanding the structure and variability of adaptive loci such as the major histocompatibility complex (MHC) genes is a primary research goal for evolutionary and conservation genetics. Typically, classical MHC genes show high polymorphism and are under strong balancing selection, as their products trigger the adaptive immune response in vertebrates. Here, we assess the allelic diversity and patterns of selection for MHC class I and class II loci in a threatened shorebird with highly flexible mating and parental care behaviour, the Snowy Plover (Charadrius nivosus) across its broad geographic range. Results We determined the allelic and nucleotide diversity for MHC class I and class II genes using samples of 250 individuals from eight breeding population of Snowy Plovers. We found 40 alleles at MHC class I and six alleles at MHC class II, with individuals carrying two to seven different alleles (mean 3.70) at MHC class I and up to two alleles (mean 1.45) at MHC class II. Diversity was higher in the peptide-binding region, which suggests balancing selection. The MHC class I locus showed stronger signatures of both positive and negative selection than the MHC class II locus. Most alleles were present in more than one population. If present, private alleles generally occurred at very low frequencies in each population, except for the private alleles of MHC class I in one island population (Puerto Rico, lineage tenuirostris). Conclusion Snowy Plovers exhibited an intermediate level of diversity at the MHC, similar to that reported in other Charadriiformes. The differences found in the patterns of selection between the class I and II loci are consistent with the hypothesis that different mechanisms shape the sequence evolution of MHC class I and class II genes. The rarity of private alleles across populations is consistent with high natal and breeding dispersal and the low genetic structure previously observed at neutral genetic markers in this species.

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Erratum: MHC class I and MHC class II DRB gene variability in wild and captive Bengal tigers (Panthera tigris tigris)

Immunogenetics ◽

10.1007/s00251-010-0496-2 ◽

2010 ◽

Vol 63 (2) ◽

pp. 121-121 ◽

Cited By ~ 1

Author(s):

Ina Pokorny ◽

Reeta Sharma ◽

Surendra Prakash Goyal ◽

Sudanshu Mishra ◽

Ralph Tiedemann

Keyword(s):

Mhc Class I ◽

Mhc Class Ii ◽

Class Ii ◽

Class I ◽

Panthera Tigris ◽

Panthera Tigris Tigris ◽

Drb Gene ◽

Gene Variability

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Molecular Characterization of MHC Class I Genes in Four Species of the Turdidae Family to Assess Genetic Diversity and Selection

BioMed Research International ◽

10.1155/2021/5585687 ◽

2021 ◽

Vol 2021 ◽

pp. 1-14

Author(s):

Muhammad Usman Ghani ◽

Li Bo ◽

An Buyang ◽

Xu Yanchun ◽

Shakeel Hussain ◽

...

Keyword(s):

Genetic Diversity ◽

Mhc Class I ◽

Gene Diversity ◽

Geographical Area ◽

Class I ◽

Mhc I ◽

Mhc Genes ◽

Histocompatibility Complex ◽

I Gene

In vertebrate animals, the molecules encoded by major histocompatibility complex (MHC) genes play an essential role in the adaptive immunity. MHC class I deals with intracellular pathogens (virus) in birds. MHC class I diversity depends on the consequence of local and global environment selective pressure and gene flow. Here, we evaluated the MHC class I gene in four species of the Turdidae family from a broad geographical area of northeast China. We isolated 77 MHC class I sequences, including 47 putatively functional sequences and 30 pseudosequences from 80 individuals. Using the method based on analysis of cloned amplicons ( n = 25 ) for each species, we found two and seven MHC I sequences per individual indicating more than one MHC I locus identified in all sampled species. Results revealed an overall elevated genetic diversity at MHC class I, evidence of different selection patterns among the domains of PBR and non-PBR. Alleles are found to be divergent with overall polymorphic sites per species ranging between 58 and 70 (out of 291 sites). Moreover, transspecies alleles were evident due to convergent evolution or recent speciation for the genus. Phylogenetic relationships among MHC I show an intermingling of alleles clustering among the Turdidae family rather than between other passerines. Pronounced MHC I gene diversity is essential for the existence of species. Our study signifies a valuable tool for the characterization of evolutionary relevant difference across a population of birds with high conservational concerns.

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Major Histocompatibility Complex (MHC) Genes and Disease Resistance in Fish

10.20944/preprints201902.0179.v1 ◽

2019 ◽

Author(s):

Takuya Yamaguchi ◽

Johannes M. Dijkstra

Keyword(s):

Disease Resistance ◽

Mhc Class I ◽

Mhc Class Ii ◽

Teleost Fish ◽

Class Ii ◽

Point Of View ◽

Class I ◽

Mhc Genes ◽

Histocompatibility Complex ◽

Genomic Regions

The basic pattern of MHC variation in fish, with MHC class I versus class II, and polymorphic classical versus nonpolymorphic nonclassical, is similar in fish and mammals. Nevertheless, in many or all teleost fishes, important differences with mammalian or human MHC were observed: (1) The allelic/haplotype diversification levels of classical MHC class I genes tend to be much higher than in mammals; (2) Teleost fish classical MHC class I and class II loci are not linked. The present article summarizes previous studies that performed quantitative trait loci (QTL) analysis for mapping differences in teleost fish disease resistance, and discusses them from MHC point of view. Overall, those studies suggest the possible importance of genomic regions including classical MHC class II and nonclassical MHC class I genes, whereas similar observations were not made for the genomic regions with the highly diversified classical MHC class I alleles. The present study is a review and discussion of the fish MHC situation.

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