scholarly journals De-novo draft genome sequence of Crocus Sativus L, Saffron, a golden condiment.

2021 ◽  
Author(s):  
Sheetal Ambardar ◽  
Jyoti Vakhlu ◽  
Ramanathan Sowdhamini
Keyword(s):  
Genome Sequence ◽  
De Novo ◽  
Economic Status ◽  
Draft Genome ◽  
Crocus Sativus ◽  
Draft Genome Sequence ◽  
Go Annotation ◽  
Kegg Pathways ◽  
Related Family ◽  
Crocus Sativus L

Crocus sativus L, saffron is the highest priced but low yielding plant of medicinal and culinary importance. Despite its economic status, the omic information on this plant is very scarce, with only a couple of transcriptomics and epigenetic studies. In the present study, the draft genome sequence of C. sativus has been assembled using Illumina sequencing. In fact, this is the first genome sequence from any member of family Iridaceae. Genome size of C. sativus was estimated to be 3.5 Gb and the draft genome is 3.01 Gb long with 84.24% genome coverage. In total, 8,62,275 repeats and 9,64,231 SSR markers have been identified. A total of 53,546 functional genes were annotated, out of which, 43,649 proteins were associated with GO annotation. 5726 proteins were identified as transcription factors, with MYB & MYB related family proteins being more abundant. Orthology analysis of C. sativus with 3 different monocot species of the same plant order and rice (model monocot plant) revealed 7328 proteins clusters to be conserved in all the five plant species, whereas 2510 proteins cluster were unique to C. sativus only. 10,912 unigenes of C. sativus were mapped to 387 KEGG pathways of monocot. The genes involved in the pathway of apocarotenoids biosynthesis (crocin, crocetin, picrocrocin, and safranal) were present in the draft genome.

scholarly journals Insights from the analysis of draft genome sequence of Crocus sativus L.

2022 ◽  
Vol 18 (1) ◽  
pp. 1-13
Author(s):  
Ramanathan Sowdhamini ◽  
Keyword(s):  
Transcription Factors ◽  
Genome Sequence ◽  
Genome Annotation ◽  
Draft Genome ◽  
Economic Importance ◽  
Crocus Sativus ◽  
Draft Genome Sequence ◽  
Functional Genes ◽  
Biosynthesis Pathway ◽  
Crocus Sativus L

Saffron (Crocus sativus L.) is the low yielding plant of medicinal and economic importance. Therefore, it is of interest to report the draft genome sequence of C. sativus. The draft genome of C. sativus has been assembled using Illumina sequencing and is 3.01 Gb long covering 84.24% of genome. C. sativus genome annotation identified 53,546 functional genes (including 5726 transcription factors), 862,275 repeats and 964,231 SSR markers. The genes involved in the apocarotenoids biosynthesis pathway (crocin, crocetin, picrocrocin, and safranal) were found in the draft genome analysis.

scholarly journals Draft Genome Sequence of Leifsonia xyli subsp. xyli Strain gdw1

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Jihua Wang ◽  
Li Wang ◽  
Gan Cao ◽  
Muqing Zhang ◽  
Ying Guo
Keyword(s):  
Genome Sequence ◽  
Genetic Improvement ◽  
De Novo ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Coding Sequences ◽  
Ratoon Stunting Disease

Here, we report the draft genome sequence of Leifsonia xyli subsp. xyli strain gdw1, isolated from the stem of Badila sugarcane located at the Guangdong Key Laboratory for Crops Genetic Improvement (Guanzhou, China), that causes ratoon stunting disease of sugarcane. The de novo genome of Leifsonia xyli subsp. xyli was assembled with 48 scaffolds and a G+C content of 67.68%, and contained 2.6 Mb bp and 2,838 coding sequences.

scholarly journals Draft Genome Sequence of the Community-Associated Staphylococcus aureus Sequence Type 88 Strain LVP-7, Isolated from an Ocular Infection

2021 ◽  
Vol 10 (7) ◽  
Author(s):  
Savitha Nadig ◽  
Sneha Murthy ◽  
Muralidharan Vandanashree ◽  
Hosahalli S. Subramanya ◽  
Balasubramanian Gopal ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
De Novo ◽  
Draft Genome ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Ocular Infection ◽  
Content Type ◽  
Type V ◽  
Panton Valentine Leukocidin

ABSTRACT We report a de novo-assembled draft genome sequence of the Indian Staphylococcus aureus sequence type 88 (ST88) strain LVP-7, isolated from an ocular infection. The genome harbors a Panton-Valentine leukocidin phage, a type V staphylococcal cassette chromosome mec element, the delta-hemolysin-converting Newman phage ΦNM3, and the pathogenicity island SaPI3, encoding the superantigen enterotoxin B.

scholarly journals Draft Genome Sequence of a New Zealand Isolate of Mycoplasma ovipneumoniae

2020 ◽  
Vol 9 (10) ◽  
Author(s):  
B. J. Bridgeman ◽  
S. K. Gupta ◽  
A. Murray ◽  
V. S. R. Dukkipati ◽  
E. Altermann ◽  
...  
Keyword(s):  
New Zealand ◽  
Genome Sequence ◽  
De Novo ◽  
Draft Genome ◽  
Illumina Miseq ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Mycoplasma Ovipneumoniae

The genome of New Zealand Mycoplasma ovipneumoniae isolate 90 was sequenced and assembled using an Illumina MiSeq system and combining the built-in Geneious de novo and Velvet de novo assemblers. The 1,031,345-bp-long genome harbored 711 genes with a coding percentage of 86.6.

scholarly journals Improved De Novo Draft Genome Sequence of the Nocavionin-Producing Type Strain Nocardia terpenica IFM 0706 and Comparative Genomics with the Closely Related Strain Nocardia terpenica IFM 0406

2020 ◽  
Vol 9 (34) ◽  
Author(s):  
Anina Buchmann ◽  
Harald Gross
Keyword(s):  
Comparative Genomics ◽  
Genome Size ◽  
Genome Sequence ◽  
Type Strain ◽  
De Novo ◽  
Draft Genome ◽  
Pathogenic Strain ◽  
Draft Genome Sequence ◽  
Related Strain ◽  
Content Type

ABSTRACT We report an improved de novo draft genome sequence of the human-pathogenic strain Nocardia terpenica IFM 0706T. The resequencing unveiled that the genome size is larger than anticipated, reducing significantly the number of contigs and building a basis for comparison with the closely related strain N. terpenica IFM 0406.

scholarly journals Draft Genome Sequence of Rheinheimera sp. F8, a Biofilm-Forming Strain Which Produces Large Amounts of Extracellular DNA

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Anna-Kathrin Schuster ◽  
Ulrich Szewzyk
Keyword(s):  
Genome Sequence ◽  
De Novo ◽  
Draft Genome ◽  
Extracellular Dna ◽  
Draft Genome Sequence ◽  
Protein Coding ◽  
Coding Sequences

Rheinheimera sp. strain F8 is a biofilm-forming gammaproteobacterium that has been found to produce large amounts of filamentous extracellular DNA. Here, we announce the de novo assembly of its genome. It is estimated to be 4,464,511 bp in length, with 3,970 protein-coding sequences and 92 RNA-coding sequences.

scholarly journals Comparative High-Density Microarray Analysis of Gene Expression during Growth of Lactobacillus helveticus in Milk versus Rich Culture Medium

2007 ◽  
Vol 73 (8) ◽  
pp. 2661-2672 ◽  
Author(s):  
Vladimir V. Smeianov ◽  
Patrick Wechter ◽  
Jeffery R. Broadbent ◽  
Joanne E. Hughes ◽  
Beatriz T. Rodríguez ◽  
...  
Keyword(s):  
Genome Sequence ◽  
De Novo ◽  
Cell Envelope ◽  
Draft Genome ◽  
Skim Milk ◽  
Defined Medium ◽  
Lactobacillus Helveticus ◽  
Draft Genome Sequence ◽  
Sequence Contigs ◽  
Cheese Flavor

ABSTRACT Lactobacillus helveticus CNRZ32 is used by the dairy industry to modulate cheese flavor. The compilation of a draft genome sequence for this strain allowed us to identify and completely sequence 168 genes potentially important for the growth of this organism in milk or for cheese flavor development. The primary aim of this study was to investigate the expression of these genes during growth in milk and MRS medium by using microarrays. Oligonucleotide probes against each of the completely sequenced genes were compiled on maskless photolithography-based DNA microarrays. Additionally, the entire draft genome sequence was used to produce tiled microarrays in which noninterrupted sequence contigs were covered by consecutive 24-mer probes and associated mismatch probe sets. Total RNA isolated from cells grown in skim milk or in MRS to mid-log phase was used as a template to synthesize cDNA, followed by Cy3 labeling and hybridization. An analysis of data from annotated gene probes identified 42 genes that were upregulated during the growth of CNRZ32 in milk (P < 0.05), and 25 of these genes showed upregulation after applying Bonferroni's adjustment. The tiled microarrays identified numerous additional genes that were upregulated in milk versus MRS. Collectively, array data showed the growth of CNRZ32 in milk-induced genes encoding cell-envelope proteinases, oligopeptide transporters, and endopeptidases as well as enzymes for lactose and cysteine pathways, de novo synthesis, and/or salvage pathways for purines and pyrimidines and other functions. Genes for a hypothetical phosphoserine utilization pathway were also differentially expressed. Preliminary experiments indicate that cheese-derived, phosphoserine-containing peptides increase growth rates of CNRZ32 in a chemically defined medium. These results suggest that phosphoserine is used as an energy source during the growth of L. helveticus CNRZ32.

scholarly journals Draft Genome Sequence of Ustilago trichophora RK089, a Promising Malic Acid Producer

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Thiemo Zambanini ◽  
Joerg M. Buescher ◽  
Guido Meurer ◽  
Nick Wierckx ◽  
Lars M. Blank
Keyword(s):  
Metabolic Engineering ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Malic Acid ◽  
De Novo ◽  
Draft Genome ◽  
Ustilago Maydis ◽  
Essential Genes ◽  
Smut Fungus ◽  
Draft Genome Sequence

The basidiomycetous smut fungus Ustilago trichophora  RK089 produces malate from glycerol. De novo genome sequencing revealed a 20.7-Mbp genome (301 gap-closed contigs, 246 scaffolds). A comparison to the genome of Ustilago maydis  521 revealed all essential genes for malate production from glycerol contributing to metabolic engineering for improving malate production.

scholarly journals Draft Genome Sequence of the Type Strain Streptomyces armeniacus ATCC 15676

2018 ◽  
Vol 7 (11) ◽  
Author(s):  
Puneet Labana ◽  
Jessica T. Gosse ◽  
Christopher N. Boddy
Keyword(s):  
Secondary Metabolite ◽  
Genome Sequence ◽  
Type Strain ◽  
De Novo ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Significant Source ◽  
Content Type

The Streptomyces genus represents a prolific and significant source for secondary metabolite discovery. Here, we report a de novo assembly and draft genome sequence for the type strain Streptomyces armeniacus ATCC 15676.

scholarly journals Draft Genome Sequence of Leifsonia sp. Strain NCR5, a Rhizobacterium Isolated from Cadmium-Contaminated Soil

2017 ◽  
Vol 5 (23) ◽  
Author(s):  
Eleonora Egidi ◽  
Jennifer L. Wood ◽  
Edward M. Fox ◽  
Wuxing Liu ◽  
Ashley E. Franks
Keyword(s):  
Genome Sequence ◽  
Contaminated Soil ◽  
De Novo ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Gram Positive ◽  
Coding Sequences ◽  
Cadmium Contaminated Soil

ABSTRACT We report here the draft genome sequence of Leifsonia sp. strain NCR5, a Gram-positive actinomycete isolated from Carpobrotus rossii (Haw.) Schwantes rhizosphere. The de novo genome of Leifsonia sp. strain NCR5 was assembled with 69 scaffolds and a G+C content of 69%, was 4.2 Mb in length, and contained 3,952 coding sequences.

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