scholarly journals In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma

2021 ◽  
Author(s):  
Akiyoshi Shimatani ◽  
Hiromitsu Toyoda ◽  
Kumi Orita ◽  
Yoshihiro Hirakawa ◽  
Kodai Aoki ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Atmospheric Pressure ◽  
Discharge Plasma ◽  
Gas Discharge ◽  
Control Group ◽  
Large Bone Defect ◽  
X Ray ◽  
Gas Discharge Plasma ◽  
Large Bone

Medical treatment using non-thermal atmospheric pressure gas discharge plasma is rapidly gaining recognition. Gas discharge plasma is thought to generate highly reactive species in an ambient atmosphere, which could be exposed to biological targets (e.g., cells and tissues). If plasma-generated reactive species could stimulate bone regeneration, gas discharge plasma could provide a new treatment opportunity in regenerative medicine. We investigated the impact of plasma on bone regeneration using a large bone defect in model rabbits and simple atmospheric pressure plasma (helium microplasma jet). We tracked the recovery progress of the large bone defects by X-ray imaging over eight weeks. The X-ray results showed a clear difference in the filling of the large bone defect among groups with different plasma treatment times, whereas filling was not substantial in the untreated control group. According to the results of micro-computed tomography analysis at eight weeks, the most successful bone regeneration was achieved using a plasma treatment time of 10 min, wherein the new bone volume was 1.51 times larger than that in the control group. Overall, these results suggest that non-thermal atmospheric pressure gas discharge plasma is promising for fracture treatment.

scholarly journals In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0255861
Author(s):  
Akiyoshi Shimatani ◽  
Hiromitsu Toyoda ◽  
Kumi Orita ◽  
Yoshihiro Hirakawa ◽  
Kodai Aoki ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Control Group ◽  
Untreated Control Group ◽  
Large Bone Defect ◽  
X Ray ◽  
Large Bone

Medical treatment using non-thermal atmospheric pressure plasma (NTAPP) is rapidly gaining recognition. NTAPP is thought to be a new therapeutic method because it could generate highly reactive species in an ambient atmosphere which could be exposed to biological targets (e.g., cells and tissues). If plasma-generated reactive species could stimulate bone regeneration, NTAPP can provide a new treatment opportunity in regenerative medicine. Here, we investigated the impact of NTAPP on bone regeneration using a large bone defect in New Zealand White rabbits and a simple atmospheric pressure plasma (helium microplasma jet). We observed the recovery progress of the large bone defects by X-ray imaging over eight weeks after surgery. The X-ray results showed a clear difference in the occupancy of the new bone of the large bone defect among groups with different plasma treatment times, whereas the new bone occupancy was not substantial in the untreated control group. According to the results of micro-computed tomography analysis at eight weeks, the most successful bone regeneration was achieved using a plasma treatment time of 10 min, wherein the new bone volume was 1.51 times larger than that in the plasma untreated control group. Using H&E and Masson trichrome stains, nucleated cells were uniformly observed, and no inclusion was confirmed, respectively, in the groups of plasma treatment. We concluded the critical large bone defect were filled with new bone. Overall, these results suggest that NTAPP is promising for fracture treatment.

scholarly journals Spectroscopic diagnostics of a high-frequency dielectric barrier discharge of atmospheric pressure in mixtures of cadmium diiodide vapor with neon and small additions of xenon

2020 ◽  
Vol 1 (1) ◽  
pp. 45-53
Author(s):  
Antonina Malinina
Keyword(s):  
Atmospheric Pressure ◽  
High Frequency ◽  
Discharge Plasma ◽  
Barrier Discharge ◽  
Spectral Characteristics ◽  
Emission Spectra ◽  
Gas Discharge ◽  
Gas Discharge Plasma ◽  
Excimer Molecules ◽  
Multi Wavelength

Diagnostics of the emission spectra of a gas-discharge plasma of a barrier discharge of atmospheric pressure in mixtures of cadmium diiodide vapor with neon and small additions of xenon is carried out. A gas-discharge plasma was created and the components of the working mixture were excited by a high-frequency barrier discharge at a sinusoidal voltage pulse repetition rate of up to 140 kHz. Emission of exciplex molecules of cadmium monoiodide with a maximum emission at a wavelength of λ = 655 nm and xenon iodide with a maximum of radiation at wavelengths λ = 253 and λ = 320 nm, excimer molecules of iodine, lines of atoms of cadmium, iodine, neon and xenon was revealed. It was found that at temperatures of the mixtures under study above 150 0C, the predominance of radiation from exciplex molecules of cadmium monoiodide is observed. The reason for the increased brightness of the emission of exciplex molecules of cadmium monoiodide in mixtures of cadmium diiodide vapor with neon and xenon additive is established. The regularities in the spectral characteristics of radiation in the mixtures under study are discussed. A high-frequency barrier discharge of atmospheric pressure in mixtures of cadmium diiodide vapor with neon and xenon is of interest for creating a multi-wavelength excilamp in the UV and visible spectral ranges

Atmospheric-pressure, nonthermal plasma sterilization of microorganisms in liquids and on surfaces

2008 ◽  
Vol 80 (9) ◽  
pp. 1953-1969 ◽  
Author(s):  
Yuri Akishev ◽  
Michail Grushin ◽  
Vladimir Karalnik ◽  
Nickolay Trushkin ◽  
Vasiliy Kholodenko ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Gas Flow ◽  
Discharge Plasma ◽  
Nonthermal Plasma ◽  
Gas Discharge ◽  
Plasma Sources ◽  
Plasma Sterilization ◽  
Gas Discharge Plasma ◽  
Promising Area ◽  
Widespread Interest

Gas discharge plasma inactivation of microorganisms at low (close to ambient) temperature is a promising area of investigation that is attracting widespread interest. This paper describes atmospheric-pressure, nonthermal plasma (NTP) methods for cold sterilization of liquids and thermal sensitive surfaces. These methods are based on the use of direct current (DC) gas discharge plasma sources fed with steady-state high voltage. Parameters characterizing the plasma sources used (plasma-forming gas, gas flow rate, electric power consumed, etc.) are given. The results for plasma sterilization of different microorganisms (vegetative cells, spores, fungi, biofilms) are presented. An empirical mathematical approach is developed for describing NTP inactivation of microorganisms. This approach takes into account not only the destruction of different components of the cells, but their reparation as well.

scholarly journals Effects of BMP-2 on neovascularization during large bone defect regeneration

2018 ◽  
Author(s):  
HB Pearson ◽  
DE Mason ◽  
CD Kegelman ◽  
L Zhao ◽  
JH Dawahare ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Limiting Factor ◽  
Bone Marrow Niche ◽  
Mesenchymal Progenitor Cells ◽  
Large Bone Defect ◽  
Endothelial Colony Forming Cells ◽  
Large Bone

AbstractInsufficient blood vessel supply is a primary limiting factor for regenerative approaches to large bone defect repair. Recombinant BMP-2 delivery induces robust bone formation and has been observed to enhance neovascularization, but whether the angiogenic effects of BMP-2 are due to direct endothelial cell stimulation or to indirect paracrine signaling remains unclear. Here, we evaluated the effects of BMP-2 delivery on vascularized bone regeneration and tested whether BMP-2 induces neovascularization directly or indirectly. We found that delivery of BMP-2 (5 μg) enhanced both bone formation and neovascularization in critically sized (8 mm) rat femoral bone defects; however, BMP-2 did not directly stimulate angiogenesis in vitro. In contrast, conditioned medium from both mesenchymal progenitor cells and osteoblasts induced angiogenesis in vitro, suggesting a paracrine mechanism of BMP-2 action. Consistent with this inference, co-delivery of BMP-2 with endothelial colony forming cells (ECFCs) to a heterotopic site, distant from the bone marrow niche, induced ossification but had no effect on neovascularization. Taken together, these data suggest that BMP-2 induces neovascularization during bone regeneration primarily through paracrine activation of osteoprogenitor cells.

Photoelectrical Properties of Semiconductor in Contact with Gas Discharge Plasma

1997 ◽  
Vol 7 (5) ◽  
pp. 1039-1044
Author(s):  
N. N. Lebedeva ◽  
V. I. Orbukh ◽  
B. G. Salamov ◽  
M. Özer ◽  
K. Çolakoǧlu ◽  
...  
Keyword(s):  
Discharge Plasma ◽  
Gas Discharge ◽  
Gas Discharge Plasma ◽  
Photoelectrical Properties
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