scholarly journals In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0255861
Author(s):  
Akiyoshi Shimatani ◽  
Hiromitsu Toyoda ◽  
Kumi Orita ◽  
Yoshihiro Hirakawa ◽  
Kodai Aoki ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Control Group ◽  
Untreated Control Group ◽  
Large Bone Defect ◽  
X Ray ◽  
Large Bone

Medical treatment using non-thermal atmospheric pressure plasma (NTAPP) is rapidly gaining recognition. NTAPP is thought to be a new therapeutic method because it could generate highly reactive species in an ambient atmosphere which could be exposed to biological targets (e.g., cells and tissues). If plasma-generated reactive species could stimulate bone regeneration, NTAPP can provide a new treatment opportunity in regenerative medicine. Here, we investigated the impact of NTAPP on bone regeneration using a large bone defect in New Zealand White rabbits and a simple atmospheric pressure plasma (helium microplasma jet). We observed the recovery progress of the large bone defects by X-ray imaging over eight weeks after surgery. The X-ray results showed a clear difference in the occupancy of the new bone of the large bone defect among groups with different plasma treatment times, whereas the new bone occupancy was not substantial in the untreated control group. According to the results of micro-computed tomography analysis at eight weeks, the most successful bone regeneration was achieved using a plasma treatment time of 10 min, wherein the new bone volume was 1.51 times larger than that in the plasma untreated control group. Using H&E and Masson trichrome stains, nucleated cells were uniformly observed, and no inclusion was confirmed, respectively, in the groups of plasma treatment. We concluded the critical large bone defect were filled with new bone. Overall, these results suggest that NTAPP is promising for fracture treatment.

scholarly journals In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma

2021 ◽  
Author(s):  
Akiyoshi Shimatani ◽  
Hiromitsu Toyoda ◽  
Kumi Orita ◽  
Yoshihiro Hirakawa ◽  
Kodai Aoki ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Atmospheric Pressure ◽  
Discharge Plasma ◽  
Gas Discharge ◽  
Control Group ◽  
Large Bone Defect ◽  
X Ray ◽  
Gas Discharge Plasma ◽  
Large Bone

Medical treatment using non-thermal atmospheric pressure gas discharge plasma is rapidly gaining recognition. Gas discharge plasma is thought to generate highly reactive species in an ambient atmosphere, which could be exposed to biological targets (e.g., cells and tissues). If plasma-generated reactive species could stimulate bone regeneration, gas discharge plasma could provide a new treatment opportunity in regenerative medicine. We investigated the impact of plasma on bone regeneration using a large bone defect in model rabbits and simple atmospheric pressure plasma (helium microplasma jet). We tracked the recovery progress of the large bone defects by X-ray imaging over eight weeks. The X-ray results showed a clear difference in the filling of the large bone defect among groups with different plasma treatment times, whereas filling was not substantial in the untreated control group. According to the results of micro-computed tomography analysis at eight weeks, the most successful bone regeneration was achieved using a plasma treatment time of 10 min, wherein the new bone volume was 1.51 times larger than that in the control group. Overall, these results suggest that non-thermal atmospheric pressure gas discharge plasma is promising for fracture treatment.

Effect of Atmospheric Pressure Plasma Treatment on Shear Bond Strength Between Zirconia and Dental Porcelain Veneer

2020 ◽  
Vol 20 (9) ◽  
pp. 5683-5685
Author(s):  
Min-Kyung Ji ◽  
Jong-Tak Lee ◽  
Eun-Kyung Yim ◽  
Chan Park ◽  
Byung-Kwon Moon ◽  
...  
Keyword(s):  
Bond Strength ◽  
Plasma Treatment ◽  
Atmospheric Pressure ◽  
Shear Bond Strength ◽  
Atmospheric Pressure Plasma ◽  
Control Group ◽  
Airborne Particle ◽  
Dental Porcelain ◽  
Pressure Plasma ◽  
Group A

Various surface treatments on zirconia have been reported for dental porcelain veneer. However, it has not been determined which of these treatments provide the highest bond strength. The purpose of this study is to compare the effect of airborne particle abrasion and atmospheric pressure plasma treatment on the shear bond strength between zirconia and dental porcelain veneer. The groups were divided into four groups according to the surface treatment method: the control group, the atmospheric pressure plasma treated group (group P), the airborne particle abrasion group (group A), the atmospheric pressure plasma treated group after the airborne particle abrasion (group AP). Atmospheric pressure plasma was applied on the specimens using a plasma generator (Plasma JET, POLYBIOTECH Co. Ltd., Gwangju, Korea) and airborne-particle abraded with 110 µm. The characteristics of surface treated zirconia were analyzed by 3D-OP, XRD, XPS and contact angle. The shear bond strength was tested using a universal testing machine. The shear bond strength of group P was significantly increased compared to that of the control group (P < 0.05). The shear bond strength of group AP was significantly increased as compared to group A (P < 0.05). There was no significant difference between the group P and group A (P > 0.05). As a result of this study, the atmospheric pressure plasma treatment showed significantly higher shear bond strength than control group, but similar to the airborne particle abrasion, and the atmospheric pressure plasma treatment after the airborne particle abrasion provided the highest shear bond strength. This study demonstrated that application atmospheric pressure plasma treatment on zirconia may be useful for increasing bond strength between zirconia and dental porcelain veneer.

Influence of Atmospheric Pressure Plasma Pre-Treatment on Sodium Bicarbonate Desizing of Polyacrylate on PET Fabrics

2011 ◽  
Vol 331 ◽  
pp. 718-721 ◽  
Author(s):  
Xu Ming Li ◽  
Yi Ping Qiu
Keyword(s):  
Plasma Treatment ◽  
Photoelectron Spectroscopy ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Sem Analysis ◽  
X Ray ◽  
Pressure Plasma ◽  
Pre Treatment ◽  
Scanning Electron

The influence of He/O2 atmospheric pressure plasma jet (APPJ) treatment on subsequent wet desizing of polyarylate from PET fabrics was studied in present paper. Scanning electron microscopy (SEM) analysis showed an increased surface roughness after the plasma treatment. And SEM results also showed that the fiber surfaces were as clean as unsized fibers surfaces after 35s treatment followed by NaHCO3 desizing. X-ray photoelectron spectroscopy (XPS) analysis indicated that oxygen-based functional groups increased for the plasma treated polyacrylate sized fabrics. The percent desizing ratio (PDR) results showed that more than 99% PDR was achieved after 65s plasma treatment followed by a 5min NaHCO3 desizing.

scholarly journals Effects of BMP-2 on neovascularization during large bone defect regeneration

2018 ◽  
Author(s):  
HB Pearson ◽  
DE Mason ◽  
CD Kegelman ◽  
L Zhao ◽  
JH Dawahare ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Limiting Factor ◽  
Bone Marrow Niche ◽  
Mesenchymal Progenitor Cells ◽  
Large Bone Defect ◽  
Endothelial Colony Forming Cells ◽  
Large Bone

AbstractInsufficient blood vessel supply is a primary limiting factor for regenerative approaches to large bone defect repair. Recombinant BMP-2 delivery induces robust bone formation and has been observed to enhance neovascularization, but whether the angiogenic effects of BMP-2 are due to direct endothelial cell stimulation or to indirect paracrine signaling remains unclear. Here, we evaluated the effects of BMP-2 delivery on vascularized bone regeneration and tested whether BMP-2 induces neovascularization directly or indirectly. We found that delivery of BMP-2 (5 μg) enhanced both bone formation and neovascularization in critically sized (8 mm) rat femoral bone defects; however, BMP-2 did not directly stimulate angiogenesis in vitro. In contrast, conditioned medium from both mesenchymal progenitor cells and osteoblasts induced angiogenesis in vitro, suggesting a paracrine mechanism of BMP-2 action. Consistent with this inference, co-delivery of BMP-2 with endothelial colony forming cells (ECFCs) to a heterotopic site, distant from the bone marrow niche, induced ossification but had no effect on neovascularization. Taken together, these data suggest that BMP-2 induces neovascularization during bone regeneration primarily through paracrine activation of osteoprogenitor cells.

Comparison of Plasma-Aided Desizing with Conventional Desizing of Polyacrylate on PET Fabrics

2011 ◽  
Vol 331 ◽  
pp. 713-717 ◽  
Author(s):  
Xu Ming Li ◽  
Yi Ping Qiu
Keyword(s):  
Plasma Treatment ◽  
Photoelectron Spectroscopy ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Atmospheric Pressure Plasma Jet ◽  
X Ray ◽  
Force Microscopy ◽  
Atomic Force ◽  
Scanning Electron

The influence of He/O2 atmospheric pressure plasma jet (APPJ) treatment on subsequent wet desizing of polyarylate on PET fabrics was studied in present paper. Atomic force microscopy (AFM) and Scanning electron microscopy (SEM) showed an increased surface roughness after the plasma treatment. SEM also showed that the fiber surfaces were as clean as unsized fibers after 35 s treatment followed by NaHCO3 desizing. X-ray photoelectron spectroscopy (XPS) analysis indicated that oxygen-based functional groups increased for the plasma treated polyacrylate sized fabrics. Compared to conventional wet desizing, plasma treatment could significantly reduce desizing time.

scholarly journals Effect of Plasma Treatment on Titanium Surface on the Tissue Surrounding Implant Material

2021 ◽  
Vol 22 (13) ◽  
pp. 6931
Author(s):  
Hitomi Tsujita ◽  
Hiroshi Nishizaki ◽  
Akiko Miyake ◽  
Seiji Takao ◽  
Satoshi Komasa
Keyword(s):  
Plasma Treatment ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Surrounding Tissue ◽  
Control Group ◽  
Pressure Plasma ◽  
Implant Placement ◽  
Implant Material ◽  
Initial Stability

Early osseointegration is important to achieve initial stability after implant placement. We have previously reported that atmospheric-pressure plasma treatment confers superhydrophilicity to titanium. Herein, we examined the effects of titanium implant material, which was conferred superhydrophilicity by atmospheric-pressure plasma treatment, on the surrounding tissue in rat femur. Control and experimental groups included untreated screws and those irradiated with atmospheric-pressure plasma using piezobrush, respectively. The femurs of 8-week-old male Sprague-Dawley rats were used for in vivo experiments. Various data prepared from the Micro-CT analysis showed results showing that more new bone was formed in the test group than in the control group. Similar results were shown in histological analysis. Thus, titanium screw, treated with atmospheric-pressure plasma, could induce high hard tissue differentiation even at the in vivo level. This method may be useful to achieve initial stability after implant placement.

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