scholarly journals Doppler Slicing for Ultrasound Super-Resolution Without Contrast Agents

2021 ◽  
Author(s):  
Avinoam Bar-Zion ◽  
Oren Solomon ◽  
Claire Rabut ◽  
David Maresca ◽  
Yonina C. Eldar ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Ultrasound Imaging ◽  
Super Resolution ◽  
Speckle Noise ◽  
Resolution Limit ◽  
Fourier Decomposition ◽  
Brain Scans ◽  
Microbubble Contrast Agents ◽  
Resolution Imaging ◽  
Vascular Structures

Much of the information needed for diagnosis and treatment monitoring of diseases like cancer and cardiovascular disease is found at scales below the resolution limit of classic ultrasound imaging. Recently introduced vascular super-localization methods provide more than a ten-fold improvement in spatial resolution by precisely estimating the positions of microbubble contrast agents. However, most vascular ultrasound scans are currently performed without contrast agents due to the associated cost, training, and post-scan monitoring. Here we show that super-resolution ultrasound imaging of dense vascular structures can be achieved using the natural contrast of flowing blood cells. Instead of relying on separable targets, we used Fourier-based decomposition to separate signals arising from the different scales of vascular structures while removing speckle noise using multi-ensemble processing. This approach enabled the use of compressed sensing for super-resolution imaging of the underlying vascular structures, improving resolution by a factor of four. Reconstruction of ultrafast mouse brain scans revealed details that could not be resolved in regular Doppler images, agreeing closely with bubble-based super-localization microscopy of the same fields of view. By combining multi-ensemble Doppler acquisitions with narrowband Fourier decomposition and computational super-resolution imaging, this approach opens new opportunities for affordable and scalable super-resolution ultrasound imaging.

Super-resolution imaging of microbubble contrast agents

2015 ◽  
Author(s):  
R. J. Eckersley ◽  
K. Christensen-Jeffries ◽  
M. X. Tang ◽  
J. V. Hajnal ◽  
P. Aljabar ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Super Resolution ◽  
Microbubble Contrast Agents ◽  
Resolution Imaging

scholarly journals Demonstration of nanoimprinted hyperlens array for high-throughput sub-diffraction imaging

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Minseop Byun ◽  
Dasol Lee ◽  
Minkyung Kim ◽  
Yangdoo Kim ◽  
Kwan Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Large Scale ◽  
Medical Science ◽  
Super Resolution ◽  
Fabrication Process ◽  
Far Field ◽  
Resolution Limit ◽  
Imaging Science ◽  
Diffraction Imaging ◽  
Resolution Imaging

Abstract Overcoming the resolution limit of conventional optics is regarded as the most important issue in optical imaging science and technology. Although hyperlenses, super-resolution imaging devices based on highly anisotropic dispersion relations that allow the access of high-wavevector components, have recently achieved far-field sub-diffraction imaging in real-time, the previously demonstrated devices have suffered from the extreme difficulties of both the fabrication process and the non-artificial objects placement. This results in restrictions on the practical applications of the hyperlens devices. While implementing large-scale hyperlens arrays in conventional microscopy is desirable to solve such issues, it has not been feasible to fabricate such large-scale hyperlens array with the previously used nanofabrication methods. Here, we suggest a scalable and reliable fabrication process of a large-scale hyperlens device based on direct pattern transfer techniques. We fabricate a 5 cm × 5 cm size hyperlenses array and experimentally demonstrate that it can resolve sub-diffraction features down to 160 nm under 410 nm wavelength visible light. The array-based hyperlens device will provide a simple solution for much more practical far-field and real-time super-resolution imaging which can be widely used in optics, biology, medical science, nanotechnology and other closely related interdisciplinary fields.

scholarly journals Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Yanxiang Ni ◽  
Bo Cao ◽  
Tszshan Ma ◽  
Gang Niu ◽  
Yingdong Huo ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Molecular Beacon ◽  
Genomic Sequence ◽  
Single Cells ◽  
Nuclear Genome ◽  
Super Resolution ◽  
Resolution Limit ◽  
Fish Method ◽  
Resolution Imaging

High-resolution visualization of short non-repetitive DNA in situ in the nuclear genome is essential for studying looping interactions and chromatin organization in single cells. Recent advances in fluorescence in situ hybridization (FISH) using Oligopaint probes have enabled super-resolution imaging of genomic domains with a resolution limit of 4.9 kb. To target shorter elements, we developed a simple FISH method that uses molecular beacon (MB) probes to facilitate the probe-target binding, while minimizing non-specific fluorescence. We used three-dimensional stochastic optical reconstruction microscopy (3D-STORM) with optimized imaging conditions to efficiently distinguish sparsely distributed Alexa-647 from background cellular autofluorescence. Utilizing 3D-STORM and only 29–34 individual MB probes, we observed 3D fine-scale nanostructures of 2.5 kb integrated or endogenous unique DNA in situ in human or mouse genome, respectively. We demonstrated our MB-based FISH method was capable of visualizing the so far shortest non-repetitive genomic sequence in 3D at super-resolution.

scholarly journals High-Resolution Optical Imaging of Zebrafish Larval Ribbon Synapse Protein RIBEYE, RIM2, and CaV 1.4 by Stimulation Emission Depletion Microscopy

2012 ◽  
Vol 18 (4) ◽  
pp. 745-752 ◽  
Author(s):  
Caixia Lv ◽  
Travis J. Gould ◽  
Joerg Bewersdorf ◽  
David Zenisek
Keyword(s):  
Super Resolution ◽  
Photoreceptor Cells ◽  
Stimulated Emission ◽  
Resolution Limit ◽  
Synaptic Ribbon ◽  
Small Spot ◽  
Ribbon Structure ◽  
Resolution Imaging ◽  
Plexiform Layers ◽  
Fish Retina

AbstractThe synaptic ribbon is a unique presynaptic structure with an intricate morphology in photoreceptors. Because of the resolution limit in conventional fluorescence microscopy, investigating ribbon protein locations has been challenging, especially in the early development stages of model animals. Here, we used stimulated emission depletion microscopy, a super-resolution imaging technique, to look at retina sections in 4 days post-fertilization (dpf) zebrafish. We observed that in photoreceptor cells, RIBEYE and RIM2 are expressed along the synaptic ribbon, with RIM2 consistently located inside of the horseshoe-shaped synaptic ribbon structure with RIBEYE located on the outside. The L-type calcium channel subunit, CACNA1F, exhibited small spot-like staining beneath the RIM2 and RIBEYE structures. Using morpholino antisense oligonucleotides to knock down RIBEYE expression, we observed fewer and shorter ribbons in the photoreceptor outer plexiform layers of 4 dpf fish retina as well as a reduction in RIM2 expression. The clustering of CACNA1F in these blind fish was no longer observed, but instead showed a diffuse expression in the photoreceptor terminal.

scholarly journals Toward optimization ofin vivosuper-resolution ultrasound imaging using size-selected microbubble contrast agents

2017 ◽  
Vol 44 (12) ◽  
pp. 6304-6313 ◽  
Author(s):  
Debabrata Ghosh ◽  
Fangyuan Xiong ◽  
Shashank R. Sirsi ◽  
Philip W. Shaul ◽  
Robert F. Mattrey ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Ultrasound Imaging ◽  
Microbubble Contrast Agents

scholarly journals Far-field super-resolution imaging of resonant multiples

2016 ◽  
Vol 2 (5) ◽  
pp. e1501439 ◽  
Author(s):  
Bowen Guo ◽  
Yunsong Huang ◽  
Anders Røstad ◽  
Gerard Schuster
Keyword(s):  
Field Data ◽  
Synthetic Data ◽  
Super Resolution ◽  
Light Detection And Ranging ◽  
Far Field ◽  
Resolution Limit ◽  
High Resolution Images ◽  
Resolution Imaging ◽  
Exploration Seismology ◽  
First Time

We demonstrate for the first time that seismic resonant multiples, usually considered as noise, can be used for super-resolution imaging in the far-field region of sources and receivers. Tests with both synthetic data and field data show that resonant multiples can image reflector boundaries with resolutions more than twice the classical resolution limit. Resolution increases with the order of the resonant multiples. This procedure has important applications in earthquake and exploration seismology, radar, sonar, LIDAR (light detection and ranging), and ultrasound imaging, where the multiples can be used to make high-resolution images.

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