scholarly journals Far-field super-resolution imaging of resonant multiples

2016 ◽  
Vol 2 (5) ◽  
pp. e1501439 ◽  
Author(s):  
Bowen Guo ◽  
Yunsong Huang ◽  
Anders Røstad ◽  
Gerard Schuster
Keyword(s):  
Field Data ◽  
Synthetic Data ◽  
Super Resolution ◽  
Light Detection And Ranging ◽  
Far Field ◽  
Resolution Limit ◽  
High Resolution Images ◽  
Resolution Imaging ◽  
Exploration Seismology ◽  
First Time

We demonstrate for the first time that seismic resonant multiples, usually considered as noise, can be used for super-resolution imaging in the far-field region of sources and receivers. Tests with both synthetic data and field data show that resonant multiples can image reflector boundaries with resolutions more than twice the classical resolution limit. Resolution increases with the order of the resonant multiples. This procedure has important applications in earthquake and exploration seismology, radar, sonar, LIDAR (light detection and ranging), and ultrasound imaging, where the multiples can be used to make high-resolution images.

scholarly journals Demonstration of nanoimprinted hyperlens array for high-throughput sub-diffraction imaging

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Minseop Byun ◽  
Dasol Lee ◽  
Minkyung Kim ◽  
Yangdoo Kim ◽  
Kwan Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Large Scale ◽  
Medical Science ◽  
Super Resolution ◽  
Fabrication Process ◽  
Far Field ◽  
Resolution Limit ◽  
Imaging Science ◽  
Diffraction Imaging ◽  
Resolution Imaging

Abstract Overcoming the resolution limit of conventional optics is regarded as the most important issue in optical imaging science and technology. Although hyperlenses, super-resolution imaging devices based on highly anisotropic dispersion relations that allow the access of high-wavevector components, have recently achieved far-field sub-diffraction imaging in real-time, the previously demonstrated devices have suffered from the extreme difficulties of both the fabrication process and the non-artificial objects placement. This results in restrictions on the practical applications of the hyperlens devices. While implementing large-scale hyperlens arrays in conventional microscopy is desirable to solve such issues, it has not been feasible to fabricate such large-scale hyperlens array with the previously used nanofabrication methods. Here, we suggest a scalable and reliable fabrication process of a large-scale hyperlens device based on direct pattern transfer techniques. We fabricate a 5 cm × 5 cm size hyperlenses array and experimentally demonstrate that it can resolve sub-diffraction features down to 160 nm under 410 nm wavelength visible light. The array-based hyperlens device will provide a simple solution for much more practical far-field and real-time super-resolution imaging which can be widely used in optics, biology, medical science, nanotechnology and other closely related interdisciplinary fields.

scholarly journals Optimising superoscillatory spots for far-field super-resolution imaging

2018 ◽  
Vol 26 (7) ◽  
pp. 8095 ◽  
Author(s):  
Katrine S. Rogers ◽  
Konstantinos N. Bourdakos ◽  
Guang Hui Yuan ◽  
Sumeet Mahajan ◽  
Edward T. F. Rogers
Keyword(s):  
Super Resolution ◽  
Far Field ◽  
Resolution Imaging

Far-field super-resolution imaging using near-field illumination by micro-fiber

2013 ◽  
Vol 102 (1) ◽  
pp. 013104 ◽  
Author(s):  
Xiang Hao ◽  
Xu Liu ◽  
Cuifang Kuang ◽  
Yanghui Li ◽  
Yulong Ku ◽  
...  
Keyword(s):  
Near Field ◽  
Super Resolution ◽  
Far Field ◽  
Resolution Imaging

scholarly journals Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Yanxiang Ni ◽  
Bo Cao ◽  
Tszshan Ma ◽  
Gang Niu ◽  
Yingdong Huo ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Molecular Beacon ◽  
Genomic Sequence ◽  
Single Cells ◽  
Nuclear Genome ◽  
Super Resolution ◽  
Resolution Limit ◽  
Fish Method ◽  
Resolution Imaging

High-resolution visualization of short non-repetitive DNA in situ in the nuclear genome is essential for studying looping interactions and chromatin organization in single cells. Recent advances in fluorescence in situ hybridization (FISH) using Oligopaint probes have enabled super-resolution imaging of genomic domains with a resolution limit of 4.9 kb. To target shorter elements, we developed a simple FISH method that uses molecular beacon (MB) probes to facilitate the probe-target binding, while minimizing non-specific fluorescence. We used three-dimensional stochastic optical reconstruction microscopy (3D-STORM) with optimized imaging conditions to efficiently distinguish sparsely distributed Alexa-647 from background cellular autofluorescence. Utilizing 3D-STORM and only 29–34 individual MB probes, we observed 3D fine-scale nanostructures of 2.5 kb integrated or endogenous unique DNA in situ in human or mouse genome, respectively. We demonstrated our MB-based FISH method was capable of visualizing the so far shortest non-repetitive genomic sequence in 3D at super-resolution.

scholarly journals Far-field unlabeled super-resolution imaging with superoscillatory illumination

APL Photonics ◽  
2020 ◽  
Vol 5 (6) ◽  
pp. 066107 ◽  
Author(s):  
Edward T. F. Rogers ◽  
Shmma Quraishe ◽  
Katrine S. Rogers ◽  
Tracey A. Newman ◽  
Peter J. S. Smith ◽  
...  
Keyword(s):  
Super Resolution ◽  
Far Field ◽  
Resolution Imaging

scholarly journals High-Resolution Optical Imaging of Zebrafish Larval Ribbon Synapse Protein RIBEYE, RIM2, and CaV 1.4 by Stimulation Emission Depletion Microscopy

2012 ◽  
Vol 18 (4) ◽  
pp. 745-752 ◽  
Author(s):  
Caixia Lv ◽  
Travis J. Gould ◽  
Joerg Bewersdorf ◽  
David Zenisek
Keyword(s):  
Super Resolution ◽  
Photoreceptor Cells ◽  
Stimulated Emission ◽  
Resolution Limit ◽  
Synaptic Ribbon ◽  
Small Spot ◽  
Ribbon Structure ◽  
Resolution Imaging ◽  
Plexiform Layers ◽  
Fish Retina

AbstractThe synaptic ribbon is a unique presynaptic structure with an intricate morphology in photoreceptors. Because of the resolution limit in conventional fluorescence microscopy, investigating ribbon protein locations has been challenging, especially in the early development stages of model animals. Here, we used stimulated emission depletion microscopy, a super-resolution imaging technique, to look at retina sections in 4 days post-fertilization (dpf) zebrafish. We observed that in photoreceptor cells, RIBEYE and RIM2 are expressed along the synaptic ribbon, with RIM2 consistently located inside of the horseshoe-shaped synaptic ribbon structure with RIBEYE located on the outside. The L-type calcium channel subunit, CACNA1F, exhibited small spot-like staining beneath the RIM2 and RIBEYE structures. Using morpholino antisense oligonucleotides to knock down RIBEYE expression, we observed fewer and shorter ribbons in the photoreceptor outer plexiform layers of 4 dpf fish retina as well as a reduction in RIM2 expression. The clustering of CACNA1F in these blind fish was no longer observed, but instead showed a diffuse expression in the photoreceptor terminal.

Sign in / Sign up

Export Citation Format

Share Document