scholarly journals Tetraspanin Cd9b plays a role in fertility in zebrafish

2021 ◽  
Author(s):  
Sarah Greaves ◽  
Katherine Marsay ◽  
Peter Monk ◽  
Henry Roehl ◽  
Lynda J Partridge
Keyword(s):  
In Vitro Fertilization ◽  
Mate Choice ◽  
Clutch Size ◽  
Male Fertility ◽  
Lower Percentage ◽  
Adult Size ◽  
Vitro Fertilization

In mice, CD9 expression on the egg is required for efficient sperm-egg fusion and no effects on ovulation or male fertility are observed in CD9 null animals. Here we show that cd9b KO zebrafish also appear to have fertility defects. In contrast to mice, fewer eggs were laid by cd9b KO zebrafish pairs and, of the eggs laid, a lower percentage were fertilised. In vitro fertilization (IVF) was used to demonstrate that factors such as courting behaviour, adult size and mate choice were not causing the unexpected decrease in clutch size. The decrease in egg numbers could be rescued by exchanging either cd9b KO partner, male or female, for a wildtype (WT) partner. However, the fertilisation defect could only be rescued by crossing a cd9b KO female with a WT male. Our results indicate that Cd9b has several roles in fish fertility, affecting both clutch size and egg fertilisation.

scholarly journals A role for KIF9 in male fertility

2020 ◽  
Author(s):  
Ken Chen ◽  
Sang Yeon Cho ◽  
Yongwei Zhang ◽  
Amanda Beck ◽  
Jeffrey E. Segall
Keyword(s):  
In Situ Hybridization ◽  
In Vitro Fertilization ◽  
Protein Level ◽  
Male Fertility ◽  
Ciliated Cells ◽  
Subtle Difference ◽  
Vitro Fertilization ◽  
Kinesin Family

AbstractA mouse was generated containing a floxed exon 3 of the gene for the kinesin family member KIF9. By in situ hybridization, expression of KIF9 mRNA was highest in the testis and was also strong in epithelia containing multi-ciliated cells such as the ependyma, bronchioles and oviduct. Deletion of the exon led to loss of KIF9 expression at the mRNA and protein level with no effect on viability. However, homozygous KIF9 knockout males were sterile. Although KIF9 knockout sperm were motile, they were unable to fertilize oocytes in an in vitro fertilization assay. Closer examination of sperm motility indicated a subtle difference in waveform. Our results suggest that KIF9 plays a role male fertility, possibly through regulation of flagellar waveforms in ciliated cells.

scholarly journals Results of in vitro fertilization cycles at the Clinic for gynecology and obstetrics, Clinical center of Nis

2012 ◽  
Vol 65 (7-8) ◽  
pp. 315-318 ◽  
Author(s):  
Dejan Mitic ◽  
Vesna Kopitovic ◽  
Jasmina Popovic ◽  
Stevan Milatovic ◽  
Marin Basic ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Clinical Pregnancy ◽  
Reproductive Age ◽  
Lower Percentage ◽  
Vitro Fertilization ◽  
Gynecology And Obstetrics ◽  
Clinical Center ◽  
Intra Cytoplasmic Sperm Injection

Introduction. Infertility affects 15-17% of reproductive age couples in our country. In vitro fertilization brought revolution in treatment of this problem, bringing hope to many couples around the world for more than 3 decades. The aim of this paper was to present results and experiences of implementation of this method of treatment at the Clinic of Gynecology and Obstetrics in Nis. Material and Methods. The study included the first 402 women who had undergone in vitro fertilization program at the Clinical Center of Nis. The data were statistically analyzed by basic descriptive methods. The main outcome measures were demographic features, cause of infertility, duration of stimulation, average gonadothropine consumption, number of oocytes per aspiration and embryos transferred, mode of conception as well as clinical pregnancy and aspiration rate. Results. The two main factors were the male infertility and tubal factor inferitily, being 51.61% and 48.39%, respectively. The classical method of in vitro fertilization constituted 72.40% of all cycles, while intra cytoplasmic sperm injection method was used in 27.60% of all cycles. The average number of embryos transferred was 2.75. The cycle cancellation rate was 15.05%. The clinical pregnancy rate per embryo transfer was 35.44%; while the live birth rate per embryo transfer was 26.53%. Discussion and Conclusion. Our success rates are comparable with those in other European countries, where for in vitro fertilization the clinical pregnancy rates per aspiration and per transfer were 29.0 and 32.4%, respectively in the observed period. For intra cytoplasmic sperm injection, the corresponding rates were 29.9 and 33.0%. The main difference from the European average was the average number of transferred embryos and lower percentage rate of intra cytoplasmic sperm injection as a method of conception.

Predictive Model for Live Birth at 12 Months After Starting In-Vitro Fertilization Treatment

MedPharmRes ◽  
2018 ◽  
Vol 2 (2) ◽  
pp. 5-20
Author(s):  
Vu Ho ◽  
Toan Pham ◽  
Tuong Ho ◽  
Lan Vuong
Keyword(s):  
In Vitro Fertilization ◽  
Live Birth ◽  
Cox Regression ◽  
Financial Burden ◽  
Oocyte Retrieval ◽  
Operating Characteristics ◽  
Vitro Fertilization ◽  
Cox Regression Analysis ◽  
Significant Difference

IVF carries a considerable physical, emotional and financial burden. Therefore, it would be useful to be able to predict the likelihood of success for each couple. The aim of this retrospective cohort study was to develop a prediction model to estimate the probability of a live birth at 12 months after one completed IVF cycle (all fresh and frozen embryo transfers from the same oocyte retrieval). We analyzed data collected from 2600 women undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) at a single center in Vietnam between April 2014 and December 2015. All patients received gonadotropin-releasing hormone (GnRH) antagonist stimulation, followed by fresh and/or frozen embryo transfer (FET) on Day 3. Using Cox regression analysis, five predictive factors were identified: female age, total dose of recombinant follicle stimulating hormone used, type of trigger, fresh or FET during the first transfer, and number of subsequent FET after the first transfer. The area under the receiver operating characteristics curve for the final model was 0.63 (95% confidence interval [CI] 0.60‒0.65) and 0.60 (95% CI 0.57‒0.63) for the validation cohort. There was no significant difference between the predicted and observed probabilities of live birth (Hosmer-Lemeshow test, p > 0.05). The model developed had similar discrimination to existing models and could be implemented in clinical practice.

Differences between antigenic determinants of pig and cat zona pellucida proteins

Reproduction ◽  
2000 ◽  
pp. 15-23 ◽  
Author(s):  
K Jewgenow ◽  
M Rohleder ◽  
I Wegner
Keyword(s):  
In Vitro Fertilization ◽  
Zona Pellucida ◽  
Antigenic Determinants ◽  
Vitro Fertilization ◽  
Contraceptive Vaccine ◽  
Sperm Binding ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Dose Dependent

Despite many efforts, the control of reproduction in feral cat populations is still a problem in urban regions around the world. Immunocontraception is a promising approach; thus the present study examined the suitability of the widely used pig zona pellucida proteins (pZP) for contraception in feral domestic cats. Purified zona pellucida proteins obtained from pig and cat ovaries were used to produce highly specific antisera in rabbits. Antibodies against pZP raised in rabbits or lions were not effective inhibitors of either in vitro sperm binding (cat spermatozoa to cat oocytes) or in vitro fertilization in cats, whereas antibodies against feline zona pellucida proteins (fZP) raised in rabbits showed a dose-dependent inhibition of in vitro fertilization. Immunoelectrophoresis, ELISA and immunohistology of ovaries confirmed these results, showing crossreactivity of anti-fZP sera to fZP and to a lesser extent to pZP, but no interaction of anti-pZP sera with fZP. It is concluded that cat and pig zonae pellucidae express a very small number of shared antigenic determinants, making the use of pZP vaccine in cats questionable. A contraceptive vaccine based on feline zona pellucida determinants will be a better choice for the control of reproduction in feral cats if immunogenity can be achieved.

Fine cryopreservation method of porcine blastocysts produced by in vitro fertilization

2014 ◽  
Author(s):  
Sung-Hun Min ◽  
Jin-Woo Kim ◽  
Yong-Hee Lee ◽  
Jae-Hyun Ahn ◽  
Geon-Yeop Do ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Cryopreservation Method
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