scholarly journals TWISTED DWARF1 regulates Arabidopsis stamen development by differential activation of ABCB-mediated auxin transport

2021 ◽  
Author(s):  
Jie Liu ◽  
Roberta Ghelli ◽  
Maura Cardarelli ◽  
Markus Geisler
Keyword(s):  
Flower Development ◽  
Auxin Transport ◽  
Anther Dehiscence ◽  
Developmental Defects ◽  
Pollen Maturation ◽  
Differential Activation ◽  
Stamen Development ◽  
Stamen Number ◽  
Rapid Elongation ◽  
Local Accumulation

AbstractDespite clear evidence that a local accumulation of auxin is likewise critical for male fertility, much less is known about the components that regulate auxin-controlled stamen development.In this study, we analyzed physiological and morphological parameters in mutants of key players of ABCB-mediated auxin transport and spatially and temporally dissected their expression on the protein level as well as auxin fluxes in the Arabidopsis stamens. Our analyses revealed that the FKBP42, TWISTED DWARF1 (TWD1), promotes stamen elongation and, to a lesser extent, anther dehiscence, as well as pollen maturation and thus is required for seed development. Most of the described developmental defects in twd1 are shared with the abcb1 abcb19 mutant, which can be attributed to the fact that TWD1 - as a described ABCB chaperon - is a positive regulator of ABCB1 and ABCB19-mediated auxin transport. However, reduced stamen number was dependent on TWD1 but not on investigated ABCBs, suggesting additional actors down-stream of TWD1. We predict an overall housekeeping function for ABCB1 during earlier stages, while ABCB19 seems to be responsible for the key event of rapid elongation at later stages of stamen development. Our data indicate that TWD1 controls stamen development by differential activation of ABCB-mediated auxin transport in the stamen.HighlightBy using a mix of phenotypical and imaging analyses, we here identify and functionally characterize a new master regulator of flower development.

scholarly journals TWISTED DWARF1 regulates Arabidopsis stamen development by differential activation of ABCB-mediated auxin transport

2021 ◽  
Author(s):  
Jie Liu ◽  
Roberta Ghelli ◽  
Maura Cardarelli ◽  
Markus Geisler
Keyword(s):  
Flower Development ◽  
Auxin Transport ◽  
Floral Organ ◽  
Developmental Defects ◽  
Pollen Maturation ◽  
Differential Activation ◽  
Stamen Development ◽  
Vegetative Tissues ◽  
Key Players ◽  
Local Accumulation

Despite clear evidence that a local accumulation of auxin is likewise critical for floral organ initiation than for vegetative tissues, much less is known about the molecular key players that regulate auxin-controlled flower development. Here, by an analysis of physiological and morphological parameters and by a spatial and temporal dissection of auxin fluxes and expression of key players of ABCB-mediated auxin transport in the Arabidopsis flower, we demonstrate a crucial role for the FKBP42, TWISTED DWARF1 (TWD1), in the regulation of flower development. Our analyses revealed that TWD1 promotes flower shape and number, stamen elongation, pollen maturation, nectary functionality and seed development. Most of the described developmental defects in twd1 are shared with the abcb1 abcb19 mutant, which can be attributed to the fact that TWD1 as a described ABCB chaperon is a positive regulator of ABCB1 and ABCB19-mediated auxin transport. We predict an overall housekeeping function for ABCB1 during earlier stages, while ABCB19 seems to be responsible for the key event of rapid elongation at later stages of stamen development. Our data indicate that TWD1 controls flower development by differential activation of ABCB-mediated auxin transport.

scholarly journals Both Induction and Morphogenesis of Cyst Nematode Feeding Cells Are Mediated by Auxin

2000 ◽  
Vol 13 (10) ◽  
pp. 1121-1129 ◽  
Author(s):  
Aska Goverse ◽  
Hein Overmars ◽  
Jan Engelbertink ◽  
Arjen Schots ◽  
Jaap Bakker ◽  
...  
Keyword(s):  
Root Formation ◽  
Auxin Transport ◽  
Infection Site ◽  
Cell Morphogenesis ◽  
Cell Wall Degrading Enzymes ◽  
Cyst Nematodes ◽  
Site Specific ◽  
Auxin Concentration ◽  
Local Accumulation

Various lines of evidence show that local changes in the auxin concentration are involved in the initiation and directional expansion of syncytia induced by cyst nematodes. Analysis of nematode infections on auxin-insensitive tomato and Arabidopsis mutants revealed various phenotypes ranging from complete inhibition of syncytium development to a decrease in hypertrophy and lateral root formation at the infection site. Specific activation of an auxin-responsive promoter confirmed the role of auxin and pointed at a local accumulation of auxin in developing syncytia. Disturbance of auxin gradients by inhibiting polar auxin transport with N-(1-naphthyl)phtalamic acid (NPA) resulted in abnormal feeding cells, which were characterized by extreme galling, massive disordered cell divisions in the cortex, and absence of radial expansion of the syncytium initial toward the vascular bundle. The role of auxin gradients in guiding feeding cell morphogenesis and the cross-talk between auxin and ethylene resulting in a local activation of cell wall degrading enzymes are discussed.

scholarly journals Intrafloral phenology of Trifolium polymorphum Poir. (Leguminosae) aerial flowers and reproductive implications

2009 ◽  
Vol 23 (3) ◽  
pp. 881-888 ◽  
Author(s):  
Gabriela Speroni ◽  
Primavera Izaguirre ◽  
Gabriel Bernardello ◽  
Jorge Franco
Keyword(s):  
Flower Development ◽  
Pollen Viability ◽  
Morphological Characteristics ◽  
Structure And Function ◽  
Anther Dehiscence ◽  
Flower Longevity ◽  
Floral Phenology ◽  
Length Width ◽  
And Function ◽  
Reproductive Events

Trifolium polymorphum is an amphicarpic species that grows in Uruguay, Argentina, Brazil, Paraguay and Chile. Underground flowers are cleistogamous, obligately autogamous and morphologically highly modified in structure and function. Aerial flowers are chasmogamous, and as mentioned in earlier literature, either allogamous or autogamous. The aim of this study is to identify flower characteristics that facilitate or prevent autogamous or allogamous processes. Floral phenology stages are thus studied in correlation with estimated models of aerial flower development, pollen viability and stigmatic receptivity in three Uruguayan T. polymorphum populations. Flower longevity and development (length, width, and anther-stigma distance), anthesis and anther dehiscence initiation, pollen viability and stigmatic receptivity are the variables correlated with floral phenology. Morphological characteristics of aerial flowers favor autogamy processes - even in pre-anthesis - but phenological characteristics tend to prevent it. The most important reproductive events occurring during flower development, that facilitate autogamous or allogamous processes, are discussed here.

scholarly journals Auxin Regulates Arabidopsis Anther Dehiscence, Pollen Maturation, and Filament Elongation

2008 ◽  
Vol 20 (7) ◽  
pp. 1760-1774 ◽  
Author(s):  
Valentina Cecchetti ◽  
Maria Maddalena Altamura ◽  
Giuseppina Falasca ◽  
Paolo Costantino ◽  
Maura Cardarelli
Keyword(s):  
Anther Dehiscence ◽  
Pollen Maturation

scholarly journals Jasmonate Signaling during Arabidopsis Stamen Maturation

2019 ◽  
Vol 60 (12) ◽  
pp. 2648-2659 ◽  
Author(s):  
Ivan F Acosta ◽  
Marine Przybyl
Keyword(s):  
Male Fertility ◽  
Pollen Viability ◽  
Testable Hypothesis ◽  
Anther Dehiscence ◽  
Cellular Functions ◽  
Stamen Development ◽  
Jasmonate Signaling ◽  
Spatiotemporal Localization ◽  
Signaling Components

Abstract The last stages of stamen development, collectively called stamen maturation, encompass pollen viability, filament elongation and anther dehiscence or opening. These processes are essential for male fertility in Arabidopsis and require the function of jasmonate signaling. There is a good understanding of jasmonate synthesis, perception and transcriptional outputs in Arabidopsis stamens. In addition, the spatiotemporal localization of jasmonate signaling components at the tissue and cellular levels has started to emerge in recent years. However, the ultimate cellular functions activated by jasmonate to promote stamen maturation remain unknown. The hormones auxin and gibberellin have been proposed to control the activation of jasmonate synthesis to promote stamen maturation, although we hypothesize that this action is rather indirect. In this review, we examine these different areas, attempt to clarify some confusing aspects found in the literature and raise testable hypothesis that may help to further understand how jasmonate controls male fertility in Arabidopsis.

scholarly journals Seedling developmental defects upon blocking CINNAMATE‐4‐HYDROXYLASE are caused by perturbations in auxin transport

2021 ◽  
Author(s):  
Ilias El Houari ◽  
Caroline Van Beirs ◽  
Helena E. Arents ◽  
Huibin Han ◽  
Alexandra Chanoca ◽  
...  
Keyword(s):  
Auxin Transport ◽  
Developmental Defects

scholarly journals Tissue-wide integration of mechanical cues promotes efficient auxin patterning

2019 ◽  
Author(s):  
João R. D. Ramos ◽  
Alexis Maizel ◽  
Karen Alim
Keyword(s):  
Auxin Transport ◽  
Mechanical Response ◽  
Circumferential Stress ◽  
Compartment Model ◽  
Tissue Mechanics ◽  
Mechanical Stresses ◽  
Mechanical Coupling ◽  
The Impact ◽  
Local Accumulation ◽  
Auxin Accumulation

New plants organs form by local accumulation of auxin, which is transported by PIN proteins that localize following mechanical stresses. As auxin itself modifies tissue mechanics, a feedback loop between tissue mechanics and auxin patterning unfolds – yet the impact of tissue-wide mechanical coupling on auxin pattern emergence remains unclear. Here, we use a hybrid model composed of a vertex model for plant tissue mechanics, and a compartment model for auxin transport to explore the collective mechanical response of the tissue to auxin patterns and how it feeds back onto auxin transport. We compare a model accounting for a tissue-wide mechanical integration to a model where mechanical stresses are averaged out across the tissue. We show that only tissue-wide mechanical coupling leads to focused auxin spots, which we show to result from the formation of a circumferential stress field around these spots, self-reinforcing PIN polarity and auxin accumulation.

Sign in / Sign up

Export Citation Format

Share Document