scholarly journals Evaluation of an optimized protocol and Illumina ARTIC V4 primer pool for sequencing of SARS-CoV-2 using COVIDSeq™ and DRAGEN™ COVID Lineage App workflow

2022 ◽  
Author(s):  
Cyndi R Clark ◽  
Matthew T Hardison ◽  
Holly N Houdeshell ◽  
Alec C Vest ◽  
Darcy A Whitlock ◽  
...  
Keyword(s):  
Public Health ◽  
Next Generation Sequencing ◽  
Superior Performance ◽  
Research Use ◽  
Public Health Response ◽  
The Public ◽  
Human Control ◽  
Health Response ◽  
Optimized Protocol ◽  
Generation Sequencing

Next-Generation Sequencing based genomic surveillance has been widely implemented for identification and tracking of emerging SARS-CoV-2 variants to guide the Public Health response to the COVID-19 pandemic. Amplicon-based assays, such as the Illumina® COVIDSeq™ Test (RUO) and COVIDSeq Assay (RUO), enable scalable sequencing of SARS-CoV-2, leveraging V3 and V4 primer designs from the ARTIC community and DRAGEN™ COVID Lineage App analysis available on Illumina BaseSpace™. We report here a comparison of COVIDSeq performance for SARS-CoV-2 genome reporting using the ARTIC V3 based primer pool (including primers for human control genes) that is provided with the COVIDSeq kit versus the ARTIC V4 based Illumina COVIDSeq V4 primer pool, using an optimized protocol and DRAGEN COVID Lineage App analysis. The data indicates that both primer pools enable robust reporting of SARS-CoV-2 variants. The Illumina COVIDSeq V4 primer pool has superior performance for SARS-CoV-2 genome reporting, particularly in samples with low virus load, and is therefore the recommended primer pool for genomic surveillance of SARS-CoV-2 for research use using COVIDSeq.

scholarly journals Effect of COVID-19 on childhood Mycoplasma pneumoniae infection in Chengdu, China

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ying Zhang ◽  
Yijie Huang ◽  
Tao Ai ◽  
Jun Luo ◽  
Hanmin Liu
Keyword(s):  
Public Health ◽  
Mycoplasma Pneumoniae ◽  
Close Contact ◽  
Serum Antibody ◽  
Pathogen Transmission ◽  
Western China ◽  
Public Health Response ◽  
The Public ◽  
Health Response ◽  
The Impact

Abstract Background Following the outbreak of the COVID-19 pandemic, a change in the incidence and transmission of respiratory pathogens was observed. Here, we retrospectively analyzed the impact of COVID-19 on the epidemiologic characteristics of Mycoplasma pneumoniae infection among children in Chengdu, one of the largest cities of western China. Method M. pneumoniae infection was diagnosed in 33,345 pediatric patients with respiratory symptoms at the Chengdu Women’s & Children’s Central Hospital between January 2017 and December 2020, based on a serum antibody titer of ≥1:160 measured by the passive agglutination assay. Differences in infection rates were examined by sex, age, and temporal distribution. Results Two epidemic outbreaks occurred between October-December 2017 and April-December 2019, and two infection peaks were detected in the second and fourth quarters of 2017, 2018, and 2019. Due to the public health response to COVID-19, the number of positive M. pneumoniae cases significantly decreased in the second quarter of 2020. The number of M. pneumoniae infection among children aged 3–6 years was higher than that in other age groups. Conclusions Preschool children are more susceptible to M. pneumoniae infection and close contact appears to be the predominant factor favoring pathogen transmission. The public health response to COVID-19 can effectively control the transmission of M. pneumoniae.

Survey on the Populationʼs Needs and the Public Health Response During Floods in Germany 2002

2007 ◽  
Vol 13 (5) ◽  
pp. 461-464 ◽  
Author(s):  
Johannes Schnitzler ◽  
Justus Benzler ◽  
Doris Altmann ◽  
Inge Mücke ◽  
Gérard Krause
Keyword(s):  
Public Health ◽  
Public Health Response ◽  
The Public ◽  
Health Response

scholarly journals Practical implications of naloxone knowledge among suburban people who use opioids

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Kristin E. Schneider ◽  
Glenna J. Urquhart ◽  
Saba Rouhani ◽  
Ju Nyeong Park ◽  
Miles Morris ◽  
...  
Keyword(s):  
Public Health ◽  
Health Department ◽  
Washington Dc ◽  
Multiple Sources ◽  
Public Health Response ◽  
Baltimore City ◽  
The Public ◽  
Health Response ◽  
The Usa ◽  
Practical Implications

Abstract Background Naloxone distribution programs have been a cornerstone of the public health response to the overdose crisis in the USA. Yet people who use opioids (PWUO) continue to face a number of barriers accessing naloxone, including not knowing where it is available. Methods We used data from 173 PWUO from Anne Arundel County, Maryland, which is located between Baltimore City and Washington, DC. We assessed the prevalence of recently (past 6 months) receiving naloxone and currently having naloxone, the type(s) of the naloxone kits received, and the perceived ease/difficultly of accessing naloxone. We also assessed participants knowledge of where naloxone was available in the community. Results One third (35.7%) of participants had recently received naloxone. Most who had received naloxone received two doses (72.1%), nasal naloxone (86.9%), and education about naloxone use (72.1%). Most currently had naloxone in their possession (either on their person or at home; 78.7%). One third (34.4%) believed naloxone was difficult to obtain in their community. Only half (56.7%) knew of multiple locations where they could get naloxone. The health department was the most commonly identified naloxone source (58.0%). Identifying multiple sources of naloxone was associated with being more likely to perceive that naloxone is easy to access. Discussion Our results suggest that additional public health efforts are needed to make PWUO aware of the range of sources of naloxone in their communities in order to ensure easy and continued naloxone access to PWUO.

The public health response to the World Trade Center disaster

2007 ◽  
pp. 19-48
Author(s):  
Timothy H. Holtz ◽  
Leighton Jessica ◽  
Sharon Balter ◽  
Don Weiss ◽  
Susan Blank ◽  
...  
Keyword(s):  
Public Health ◽  
World Trade ◽  
World Trade Center ◽  
Public Health Response ◽  
World Trade Center Disaster ◽  
The Public ◽  
Health Response ◽  
The World ◽  
Trade Center
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