scholarly journals Priming winter wheat seeds with the bacterial quorum sensing signal N-hexanoyl-L-homoserine lactone (C6-HSL) shows potential to improve plant growth and seed yield

2018 ◽  
Author(s):  
Olena Moshynets ◽  
Lidia M. Babenko ◽  
Sergiy P. Rogalsky ◽  
Olga S. Iungin ◽  
Jessica Foster ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Winter Wheat ◽  
Seed Priming ◽  
Homoserine Lactone ◽  
Field Trials ◽  
Growth And Yield ◽  
Cereal Crop ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum

Several model plants are known to respond to bacterial quorum sensing molecules with altered root growth and gene expression patterns and induced resistance to plant pathogens. These compounds may represent novel elicitors that could be applied as seed primers to enhance cereal crop resistance to pathogens and abiotic stress and to improve yields. We investigated whether the acyl-homoserine lactone N-hexanoyl-L-homoserine lactone (C6-HSL) impacted winter wheat (Triticum aestivum L.) seed germination, plant development and productivity, using two Ukrainian varieties, Volodarka and Yatran 60, in both in vitro experiments and field trials. In vitro germination experiments indicated that C6-HSL seed priming had a small but significant positive impact on germination levels (1.2x increase, p < 0.0001), coleoptile and radicle development (1.4x increase, p < 0.0001). Field trials over two growing seasons (2015-16 and 2016-17) also demonstrated significant improvements in biomass at the tillering stage (1.4x increase, p < 0.0001), and crop structure and productivity at maturity including grain yield (1.4 – 1.5x increase, p < 0.0007) and quality (1.3x increase in good grain, p < 0.0001). In some cases variety effects were observed (p ≤ 0.05) suggesting that the effect of C6-HSL seed priming might depend on plant genetics, and some benefits of priming were also evident in F1 plants grown from seeds collected the previous season (p ≤ 0.05). These field-scale findings suggest that bacterial acyl-homoserine lactones such as C6-HSL could be used to improve cereal crop growth and yield and reduce reliance on fungicides and fertilisers to combat pathogens and stress.

scholarly journals Priming winter wheat seeds with the bacterial quorum sensing signal N-hexanoyl-L-homoserine lactone (C6-HSL) shows potential to improve plant growth and seed yield

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0209460 ◽  
Author(s):  
Olena V. Moshynets ◽  
Lidia M. Babenko ◽  
Sergiy P. Rogalsky ◽  
Olga S. Iungin ◽  
Jessica Foster ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Plant Growth ◽  
Winter Wheat ◽  
Seed Yield ◽  
Homoserine Lactone ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Wheat Seeds

scholarly journals Host modification of a bacterial quorum-sensing signal induces a phenotypic switch in bacterial symbionts

2017 ◽  
Vol 114 (40) ◽  
pp. E8488-E8497 ◽  
Author(s):  
Cleo Pietschke ◽  
Christian Treitz ◽  
Sylvain Forêt ◽  
Annika Schultze ◽  
Sven Künzel ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Bacterial Communities ◽  
Homoserine Lactone ◽  
Innate Immune ◽  
Expression Data ◽  
Phenotypic Switch ◽  
Host Colonization ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Quorum Sensing Signals

Bacterial communities colonize epithelial surfaces of most animals. Several factors, including the innate immune system, mucus composition, and diet, have been identified as determinants of host-associated bacterial communities. Here we show that the early branching metazoan Hydra is able to modify bacterial quorum-sensing signals. We identified a eukaryotic mechanism that enables Hydra to specifically modify long-chain 3-oxo-homoserine lactones into their 3-hydroxy-HSL counterparts. Expression data revealed that Hydra’s main bacterial colonizer, Curvibacter sp., responds differentially to N-(3-hydroxydodecanoyl)-l-homoserine lactone (3OHC12-HSL) and N-(3-oxododecanoyl)-l-homoserine lactone (3OC12-HSL). Investigating the impacts of the different N-acyl-HSLs on host colonization elucidated that 3OHC12-HSL allows and 3OC12-HSL represses host colonization of Curvibacter sp. These results show that an animal manipulates bacterial quorum-sensing signals and that this modification leads to a phenotypic switch in the bacterial colonizers. This mechanism may enable the host to manipulate the gene expression and thereby the behavior of its bacterial colonizers.

Response of Arabidopsis thaliana to N-hexanoyl-dl-homoserine-lactone, a bacterial quorum sensing molecule produced in the rhizosphere

Planta ◽  
2008 ◽  
Vol 229 (1) ◽  
pp. 73-85 ◽  
Author(s):  
Uta von Rad ◽  
Ilona Klein ◽  
Petre I. Dobrev ◽  
Jana Kottova ◽  
Eva Zazimalova ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Quorum Sensing ◽  
Homoserine Lactone ◽  
Bacterial Quorum Sensing ◽  
Bacterial Quorum ◽  
Quorum Sensing Molecule

scholarly journals Rapid Screening of Quorum-Sensing Signal N-Acyl Homoserine Lactones by an In Vitro Cell-Free Assay

2008 ◽  
Vol 74 (12) ◽  
pp. 3667-3671 ◽  
Author(s):  
Tomohiro Kawaguchi ◽  
Yung Pin Chen ◽  
R. Sean Norman ◽  
Alan W. Decho
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Quorum Sensing ◽  
High Sensitivity ◽  
Homoserine Lactone ◽  
Assay System ◽  
Rapid Screening ◽  
Whole Cell ◽  
Bacterial Quorum ◽  
Whole Cell Biosensor

ABSTRACT A simple, sensitive, and rapid cell-free assay system was developed for detection of N-acyl homoserine lactone (AHL) autoinducers involved in bacterial quorum sensing (QS). The present approach improves upon previous whole-cell biosensor-based approaches in its utilization of a cell-free assay approach to conduct bioassays. The cell-free assay was derived from the AHL biosensor bacterium Agrobacterium tumefaciens NTL4(pCF218)(pCF372), allowing the expression of β-galactosidase upon addition of exogenous AHLs. We have shown that β-galactosidase expression is possible in cell-free solution [lysate from Agrobacterium tumefaciens NTL4(pCF218)(pCF372) culture]. Assay detection limits with the use of chromogenic substrate X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) ranged from approximately 100 nM to 300 nM depending on the specific AHL. Replacement (of X-Gal) with the luminescent substrate Beta-Glo increased sensitivity to AHLs by 10-fold. A major advantage of the cell-free assay system is elimination of time-consuming steps for biosensor cell culture conditioning, which are required prior to whole-cell bioassays. This significantly reduced assay times from greater than 24 h to less than 3 h, while maintaining high sensitivity. Assay lysate may be prepared in bulk and stored (−80°C) over 6 months for future use. Finally, the present protocol may be adapted for use with other biosensor strains and be used in high-throughput AHL screening of bacteria or metagenomic libraries.

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