scholarly journals Enhanced biodegradation of naphthalene byPseudomonassp. consortium immobilized in calcium alginate beads

2019 ◽  
Author(s):  
Kunal Dutta ◽  
Sergey Shityakov ◽  
Ibrahim Khalifa ◽  
Saroj Ballav ◽  
Debarati Jana ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pseudomonas Putida ◽  
Calcium Alginate ◽  
Alginate Beads ◽  
Common Mutation ◽  
List Type ◽  
Amino Acid Residues ◽  
Potential Health ◽  
Calcium Alginate Beads ◽  
Naphthalene Biodegradation

AbstractPolycyclic aromatic hydrocarbons (PAHs) belong to a large group of organic pollutant which considers as a potential health hazard to living beings. Herein, naphthalene biodegradation potential by free and immobilizedPseudomonas putidastrain KD10 andPseudomonassp. consortium were studied. Additionally, naphthalene 1, 2-dioxygenase (nahAc) was sequenced and analyzed, which reveals two altered amino acid residues. However, the altered amino acid residues are not present in the vicinity of the active site. The gas-phase binding free energy (ΔGLondon) of the mutant variant of naphthalene 1, 2-dioxygenase was -7.10 kcal mol-1which closely resembles the wild type variant. Naphthalene biodegradation rate byPseudomonas putidastrain KD10 was 79.12 mg L-1day-1and it was significantly elevated up to 123 mg L-1day-1by the immobilizedPseudomonassp. consortium. The half-life (t1/2) for naphthalene biodegradation was 3.1 days with the inhibition constant (ki), substrate saturation constant (ks) and maximum specific degradation rate constant (qmax) of 1268 mg L-1, 395.5 mg L-1and 0.65 h-1, respectively, for thePseudomonas putidastrain KD10. However, the t1/2value was significantly reduced to 2 days along withki,ksandqmaxvalues of 1475 mg L-1, 298.8 mg L-1and 0.71 h-1, respectively, by the immobilizedPseudomonassp. consortium. The GC-MS data suggest that KD10 might follow D-gluconic acid mediated meta-cleavage pathway of catechol biodegradation. It is concluded that naphthalene biodegradation performance by immobilizedPseudomonassp. consortium was superior to free or immobilizedPseudomonas putidaKD10. Microbial consortium immobilization could be a useful tool for water quality management and environmental remediation.HighlightsSuperior naphthalene biodegradation byPseudomonassp. consortium immobilized in calcium alginate beads.A common mutation prone amino acid stretch inside chain A of naphthalene 1, 2-dioxygenase has been identified.A new naphthalene biodegradation pathway byPseudomonas putidastrain KD10 has been proposed.

Kinetics studies of p-cresol biodegradation by using Pseudomonas putida in batch reactor and in continuous bioreactor packed with calcium alginate beads

2010 ◽  
Vol 62 (12) ◽  
pp. 2920-2929 ◽  
Author(s):  
A. K. Mathur ◽  
Shashi Bala ◽  
C. B. Majumder ◽  
S. Sarkar
Keyword(s):  
Pseudomonas Putida ◽  
Calcium Alginate ◽  
Degradation Rate ◽  
Batch Reactor ◽  
Packed Bed ◽  
Alginate Beads ◽  
Calcium Alginate Beads ◽  
Continuous Bioreactor ◽  
Haldane Model ◽  
Modified Equation

Present study deals with the biodegradation of p-cresol by using Pseudomonas putida in a batch reactor and a continuous bioreactor packed with calcium alginate beads. The maximum specific growth rate of 0.8121 h−1 was obtained at 200 mg L−1 concentration of p-cresol in batch reactor. The maximum p-cresol degradation rate was obtained 6.598 mg L−1 h−1 at So=200 mg L−1 and 62.8 mg L−1 h−1 at So=500 mg L−1 for batch reactor and a continuous bioreactor, respectively. The p-cresol degradation rate of continuous bioreactor was 9 to 10-fold higher than those of the batch reactor. It shows that the continuous bioreactor could tolerate a higher concentration of p-cresol. A Haldane model was also used for p-cresol inhibition in batch reactor and a modified equation similar to Haldane model for continuous bioreactor. The Haldane parameters were obtained as μmax 0.3398 h−1, Ks 110.9574 mg L−1, and KI 497.6169 mg L−1 in batch reactor. The parameters used in continuous bioreactor were obtained as Dmax 91.801 mg L−1 h−1, Ks 131.292 mg L−1, and KI 1217.7 mg L−1. The value KI of continuous bioreactor is approximately 2.5 times higher than the batch reactor. Higher KI value of continuous bioreactor indicates P. putida can grow at high range of p-cresol concentration. The ability of tolerance of higher p-cresol concentrations may be one reason for biofilm attachment on the packed bed in the continuous operation.

scholarly journals The Enzymatic Decolorization of Textile Dyes by the Immobilized Polyphenol Oxidase from Quince Leaves

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Gulnur Arabaci ◽  
Ayse Usluoglu
Keyword(s):  
Polyphenol Oxidase ◽  
Calcium Alginate ◽  
Enzyme Stability ◽  
Industrial Effluent ◽  
Alginate Beads ◽  
Textile Dyes ◽  
Kinetic Properties ◽  
Synthetic Dyes ◽  
Cydonia Oblonga ◽  
Calcium Alginate Beads

Water pollution due to release of industrial wastewater has already become a serious problem in almost every industry using dyes to color its products. In this work, polyphenol oxidase enzyme from quince (Cydonia Oblonga) leaves immobilized on calcium alginate beads was used for the successful and effective decolorization of textile industrial effluent. Polyphenol oxidase (PPO) enzyme was extracted from quince (Cydonia Oblonga) leaves and immobilized on calcium alginate beads. The kinetic properties of free and immobilized PPO were determined. Quince leaf PPO enzyme stability was increased after immobilization. The immobilized and free enzymes were employed for the decolorization of textile dyes. The dye solutions were prepared in the concentration of 100 mg/L in distilled water and incubated with free and immobilized quince (Cydonia Oblonga) leaf PPO for one hour. The percent decolorization was calculated by taking untreated dye solution. Immobilized PPO was significantly more effective in decolorizing the dyes as compared to free enzyme. Our results showed that the immobilized quince leaf PPO enzyme could be efficiently used for the removal of synthetic dyes from industrial effluents.

scholarly journals Improving the Stability of Astaxanthin by Microencapsulation in Calcium Alginate Beads

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153685 ◽  
Author(s):  
Shen-Fu Lin ◽  
Ying-Chen Chen ◽  
Ray-Neng Chen ◽  
Ling-Chun Chen ◽  
Hsiu-O Ho ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Alginate Beads ◽  
Calcium Alginate Beads ◽  
The Stability

Calcium alginate beads motion in a foaming three-phase bubble column

2017 ◽  
Vol 324 ◽  
pp. 358-369 ◽  
Author(s):  
Gabriel Salierno ◽  
Mauricio Maestri ◽  
Stella Piovano ◽  
Miryan Cassanello ◽  
María Angélica Cardona ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Bubble Column ◽  
Alginate Beads ◽  
Calcium Alginate Beads ◽  
Three Phase

Size control of calcium alginate beads containing living cells using micro-nozzle array

Biomaterials ◽  
2005 ◽  
Vol 26 (16) ◽  
pp. 3327-3331 ◽  
Author(s):  
Shinji Sugiura ◽  
Tatsuya Oda ◽  
Yasuhiko Izumida ◽  
Yasuyuki Aoyagi ◽  
Mitsuo Satake ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Size Control ◽  
Alginate Beads ◽  
Living Cells ◽  
Calcium Alginate Beads ◽  
Micro Nozzle
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