A study and comparison of the modes of diffusion combustion of hydrogen and methane at their outflow from the annular nozzle together with the flow of supplied air from the coaxially located circular nozzle

The article presents qualitative data on the study of the process of diffusion combustion during the outflow of a gas jet from a nozzle apparatus with a certain arrangement of nozzles. The nozzle apparatus is a round nozzle with a straight channel and a coaxially located annular slot. In the experiments, hydrogen or methane was supplied through an annular slot, and the air was supplied through a central circular micro nozzle. The main features of the diffusion combustion of hydrogen and methane during the outflow from the nozzle apparatus are revealed and a qualitative comparison of the processes is carried out. In both cases, at the initial stage, laminar combustion is observed near the nozzle exit and a breakthrough of the flame front occurs with the release of an incombustible mixture of combustible gas and air. At a high flow rate, the flame separates from the nozzle exit. The fundamental difference is that hydrogen exhibits significantly better combustion stabilization characteristics at the nozzle exit.

A Parametric Study On the Fluid Dynamics and Performance Characteristic of Micro-Nozzle Flows

The present study investigates the fluid dynamics and performance characteristics in micro nozzle flows with changes in various geometric parameters using Navier-Stokes simulation based on slip wall boundary conditions. The various geometric parameters considered for the study are 1) area ratio with fixed throat dimension and 2) the semi-divergence angle variation with no change in area ratio. The simulation results show that the flow choking for micro nozzle happens not at the geometric throat; rather pushed downstream to the divergent channel of the nozzle. This is due to the thick boundary layer growth which reduces the effective flow area and shifts the minimum allowable flow area downstream to the throat. The distance to which the choking point shifts downstream to the throat reduces with Maxwell's slip wall conditions compared to the conventional no-slip wall condition. The downstream movement of the choking point from the throat reduces with an increase in area ratio and with increase in divergence angle with fixed area ratio. This is due the fact that the increase in area ratio and divergence angle increases the nozzle height at any particular section in the divergent portion of the nozzle. As a result of this, the boundary layer profile also moves upward and the restriction of potential core by the thick boundary layer reduces which in turn leads to an increase in the effective minimum flow area downstream to the throat.

Thrust Augmentation of Micro-Resistojets by Steady Micro-Jet Blowing into Planar Micro-Nozzle

The present work investigates the impact of steady micro-jet blowing on the performance of a planar micro-nozzle designed for both liquid micro-thrusters and nitrogen cold-gas micro-resistojets. Two micro-injectors have been placed into the divergent region along the sidewalls, injecting a secondary flow of propellant perpendicularly to the wall where they have been located. The micro-jet actuator configuration is characterized by the dimensionless momentum coefficient cμ. The best performance improvement is retrieved at the maximum cμ for both water vapor (Δ%T,jet = +22.6% and Δ%Isp,Tjet = +2.9% at cμ = 0.168) and nitrogen gaseous flows (Δ%T,jet = +36.1% and Δ%Isp,Tjet = +9.1% at cμ = 0.297). The fields of the Mach number and the Schlieren computations, in combination with the streamline visualization, reveal the formation of two vortical structures in the proximity of secondary jets, which energize the core flow and enhance the expansion process downstream secondary jets. The compressible momentum thickness along the width-wise direction θxy in presence of secondary injection reduces as a function of cμ. In particular, it becomes smaller than the one computed for the baseline configuration at cμ > 0.1, decreasing up to about and -57% for the water vapor flow at cμ = 0.168, and -64% for the nitrogen gaseous flow at cμ = 0.297.

High-Throughput Synthesis of Liposome Using an Injection-Molded Plastic Micro-Fluidic Device

For mass production of liposomes, we designed a plastic micro-channel device on the basis of 5 μm of micro-nozzle array forming T-junction with 100 μm depth of micro-channel. A micro-channel unit for synthesizing liposomes consisted of two micro-nozzle arrays for mixing two solutions as well as delivery and recovery channels for supplying solutions and collecting liposome suspension. The number of micro-nozzles was approximately 2400 for a micro-channel unit, and seven units were applied independently on a micro-channel plate. The plastic micro-channel plate was injection-molded for mass production using a micro-channel stamper previously fabricated by UV lithography and nickel electroforming process. A plastic cover plate with seven pairs of inlet and outlet ports was machined by mechanical milling and drilling and was assembled with a micro-channel plate using a holder to form a liposome synthesizing device. Flow and mixing of solutions in the micro-channels were tested using colored water to check the micro-fluidic characteristics of the device. Finally, a L-α-phosphatidylcholine (SOY PC) liposome was synthesized using EtOH solution of SOY PC (95%) and saline (0.85% NaOH solution) to find that the liposomes were around 230 and 260 nm in diameter, depending on the flow rate of the lipid solution.

Establishment of a Mouse Ovarian Cancer and Peritoneal Metastasis Model to Study Intraperitoneal Chemotherapy

Intraperitoneal chemotherapy (IPC) is a locoregional treatment option in patients with peritoneal metastases (PM). Here, we present an ovarian cancer (OC)-derived PM mouse model for the study of different forms of IPC. Xenograft cell proliferation (luciferase-transfected OVCAR3 and SKOV3 clones) and growth kinetics were assessed using PET scan, bioluminescence imaging (BLI), and histological tumor analysis. Liquid IPC was achieved by intraperitoneal injection with/without capnoperitoneum (6–7 mmHg). Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was mimicked using an intratracheal drug aerosol administration system (micro-nozzle), which, as demonstrated by ex vivo granulometric analysis using laser diffraction spectrometry, produced a polydisperse, bimodal aerosol with a volume-weighted median diameter of (26.49 ± 2.76) µm. Distribution of Tc-99m-labeled doxorubicin in mice was characterized using SPECT and was dependent on the delivery mode and most homogeneous when the micro-nozzle was used. A total of 2 mg doxorubicin per kg body weight was determined to be the optimally effective and tolerable dose to achieve at least 50% tumor reduction. Repeated PIPAC (four times at seven-day-intervals) with doxorubicin in SKOV3-luc tumor-bearing mice resulted in halted tumor proliferation and tumor load reduced after the second round of PIPAC versus controls and the number of tumor nodules was significantly reduced (27.7 ± 9.5 vs. 57.3 ± 9.5; p = 0.0003). Thus, we established the first mouse model of OC PM for the study of IPC using a human xenograft with SKOV3 cells and an experimental IPC setup with a miniaturized nozzle. Repeated IPC was feasible and demonstrated time-dependent anti-tumor activity.

Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles

Here, we propose a novel and simple method to efficiently capture the diffusion of fluorescein isothiocyanate (FITC)-dextran from a biocompatible substance and load the drug only to the tip of DNA microneedles. A dispensing and suction method was chosen to fabricate the designed microneedles with efficient amounts of FITC as the drug model. Importantly, the vacuum process, which could influence the capturing of FITC diffusion from the tip, was evaluated during the manufacturing process. In addition, the simulations were consistent with the experimental results and showed apparent diffusion. Moreover, dextrans of different molecular weights labeled with FITC were chosen to fabricate the tip of microneedles for demonstrating their applicability. Finally, a micro-jetting system with a micro-nozzle (diameter: 80 μm) was developed to achieve the accurate and rapid loading of small amounts of FITC using the anti-diffusion and micro-jetting methods. Our method not only uses a simple and fast manufacturing process, but also fabricates the tips of microneedles more efficiently with FITC compared with the existing methods. We believe that the proposed method is essential for the clinical applications of the microneedle drug delivery platform.

Morphofunctional Changes in Basophilic Cells of the Adenohypophysis during Post-resuscitation Disease

In the post-resuscitation period through the I-II-III-IV-V - stages and long - term periods (1 and 3 months) of post – resuscitation disease, the morpho functional activity of β-and d-basophil cells of the adenohypophysis was studied in white male rats with a weight of 180-220 g, who suffered a 10-minute stop of systemic circulation (compression of the vascular bundle of the heart by the method of V. G. Korpachev). False-operated animals served as a control. Morphological, morphometric, histochemical, and cytophotometric studies of the state of both β - and d - basophil cells of the adenohypophysis were performed. We used image analysis using a leys microscope with an electronic micro-nozzle attached to an Intel computer, and the content of the glycoprotein was studied using a cytophotometer. In the postoperative period during the phase I identified in the background of gipergidratace cytoplasmic β - and d - basophilic cells of the anterior pituitary, in the period of II-III stage disease gipergidratace nuclei β - and d - basophilic cells of the anterior pituitary revealed an increase in the excretion of glycoprotein in blood, i.e., the observed compensatory-adaptive reactions. Starting from stage IV and in remote periods of post-resuscitation disease, compensatory and restorative processes were observed against the background of hyperhydration of the nuclei.