scholarly journals Differential pulmonary immunopathologic response of domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) to Mycoplasma ovipneumoniae infection: a retrospective study

2019 ◽  
Author(s):  
Paige C. Grossman ◽  
David A. Schneider ◽  
Donald P. Knowles ◽  
Margaret A. Highland
Keyword(s):  
Retrospective Study ◽  
Lung Tissue ◽  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Respiratory Pathogen ◽  
Domestic Sheep ◽  
Lung Sample ◽  
Ffpe Tissues ◽  
Mycoplasma Ovipneumoniae

AbstractMycoplasma ovipneumoniae is a respiratory pathogen that can impact domestic sheep (Ovis aries; DS) and bighorn sheep (Ovis canadensis; BHS). Experimental and field data have indicated BHS are more susceptible than DS to developing polymicrobial pneumonia associated with Mycoplasma ovipneumoniae infection. We hypothesized that DS and BHS have a differential immunopathologic pulmonary response to M. ovipneumoniae infection. A retrospective study was performed using formalin-fixed, paraffin-embedded (FFPE) lung tissue from DS and BHS without and with M. ovipneumoniae detected in the lung tissue (n=8 per group). While each M. ovipneumoniae positive lung sample had microscopic changes typical of infection, including hyperplasia of intrapulmonary bronchus-associated lymphoid tissue (BALT) and respiratory epithelium, DS exhibited a more robust and well-organized BALT formation as compared to BHS. Immunohistochemistry was performed with antibodies reactive in FFPE tissues and specific for leukocyte and cytokine markers: T cell marker CD3, B cell markers CD20 and CD79a, macrophage markers CD163 and Iba1, and cytokine IL-17. Digital analysis was used to quantitate chromogen deposition in regions of interest (ROIs), including alveolar and bronchiolar areas, and bronchiolar subregions (epithelium and BALT). Main effects and interaction of species and infection status were analyzed by beta regression and Bonferroni corrections were performed on pairwise comparisons (PBon<0.05 significance). Significant species differences were identified for bronchiolar CD3 (PBon=0.0023) and CD163 (PBon=0.0224), alveolar CD163 (PBon=0.0057), and for IL-17 in each of the ROIs (alveolar: PBon=0.0009; BALT: PBon=0.0083; epithelium: PBon=0.0007). Infected BHS had a higher abundance of bronchiolar CD3 (PBon=0.0005) and CD163 (PBon=0.0162), and alveolar CD163 (PBon=0.0073). While IL-17 significantly increased with infection in BHS BALT (PBon=0.0179) and alveolar (0.0006) ROIs, abundance in DS showed an insignificant decrease in these ROIs and a significant decrease in epithelial abundance (PBon=0.0019). These findings support the hypothesis that DS and BHS have a differential immunopathologic response to M. ovipneumoniae infection.

scholarly journals Survival of Bighorn Sheep (Ovis canadensis) Commingled with Domestic Sheep (Ovis aries) in the Absence of Mycoplasma ovipneumoniae

2012 ◽  
Vol 48 (1) ◽  
pp. 168-172 ◽  
Author(s):  
Thomas E. Besser ◽  
E. Frances Cassirer ◽  
Catherine Yamada ◽  
Kathleen A. Potter ◽  
Caroline Herndon ◽  
...  
Keyword(s):  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Mycoplasma Ovipneumoniae

scholarly journals Differences in leukocyte differentiation molecule abundances on domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) neutrophils identified by flow cytometry

2016 ◽  
Vol 46 ◽  
pp. 40-46 ◽  
Author(s):  
Margaret A. Highland ◽  
David A. Schneider ◽  
Stephen N. White ◽  
Sally A. Madsen-Bouterse ◽  
Donald P. Knowles ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Domestic Sheep

Identification of novel variants of interferon-tau gene in Bighorn sheep (Ovis canadensis mexicana), Pelibuey sheep (Ovis aries) and its expression in hybrid blastocysts (Ovis canadensis × Ovis aries)

2012 ◽  
Vol 92 (3) ◽  
pp. 275-283 ◽  
Author(s):  
M. Palma-Irizarry ◽  
P. Damian-Matsumura ◽  
M. Betancourt ◽  
S. Romo ◽  
G. Perales ◽  
...  
Keyword(s):  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Interferon Tau ◽  
Pelibuey Sheep ◽  
Novel Variants ◽  
Ovis Canadensis Mexicana

Palma-Irizarry, M., Damian-Matsumura, P., Betancourt, M., Romo, S., Perales, G., Lagunas-Martínez, A., Madrid-Marina, M. V., Mejía, O. and Ducolomb, Y. 2012. Identification of novel variants of interferon-tau gene in Bighorn sheep ( Ovis canadensis mexicana ), Pelibuey sheep ( Ovis aries ) and its expression in hybrid blastocysts ( Ovis canadensis×Ovis aries ). Can. J. Anim. Sci. 92: 275–283. The major signal for maternal recognition of pregnancy (MRP) in ruminants is interferon-tau (IFNT). Four novel sequences of the IFNT gene were isolated from genomic DNA obtained from blood samples of Bighorn, Pelibuey or the hybrid ewes. The deduced amino acid sequence of the hybrid ewe showed 100% identity with one Bighorn sheep IFNT sequence, suggesting that this sequence was inherited from Bighorn sheep. The high degree of identity between Pelibuey and Bighorn sheep sequences could be a possible explanation for the successful MRP in some Bighorn hybrid pregnancies. In vivo and in vitro domestic sheep (Ovis aries) and hybrid sheep (Ovis aries×Ovis canadensis) blastocysts were obtained, and IFNT expression was measured by semiquantitative RT-PCR. In vivo and in vitro hybrid blastocysts showed lower expression of IFNT when compared with domestic blastocysts. In conclusion this study showed that there exists an effect of hybridization on IFNT expression and this can affect the MRP.

scholarly journals Shared Bacterial and Viral Respiratory Agents in Bighorn Sheep (Ovis canadensis), Domestic Sheep (Ovis aries), and Goats (Capra hircus) in Montana

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
David S. Miller ◽  
Glen C. Weiser ◽  
Keith Aune ◽  
Brent Roeder ◽  
Mark Atkinson ◽  
...  
Keyword(s):  
Disease Outbreaks ◽  
Bighorn Sheep ◽  
Cross Sectional Study ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Capra Hircus ◽  
Domestic Sheep ◽  
Infectious Agents ◽  
Cross Sectional ◽  
The Impact

Transmission of infectious agents from livestock reservoirs has been hypothesized to cause respiratory disease outbreaks in bighorn sheep (Ovis canadensis), and land management policies intended to limit this transmission have proven controversial. This cross-sectional study compares the infectious agents present in multiple populations of bighorn sheep near to and distant from their interface with domestic sheep (O. aries) and domestic goat (Capra hircus) and provides critical baseline information needed for interpretations of cross-species transmission risks. Bighorn sheep and livestock shared exposure to Pasteurellaceae, viral, and endoparasite agents. In contrast, although the impact is uncertain,Mycoplasmasp. was isolated from livestock but not bighorn sheep. These results may be the result of historic cross-species transmission of agents that has resulted in a mosaic of endemic and exotic agents. Future work using longitudinal and multiple population comparisons is needed to rigorously establish the risk of outbreaks from cross-species transmission of infectious agents.

scholarly journals Differential Expression of Interleukin-8 by Polymorphonuclear Leukocytes of Two Closely Related Species, Ovis canadensis and Ovis aries, in Response to Mannheimia haemolytica Infection

2010 ◽  
Vol 78 (8) ◽  
pp. 3578-3584 ◽  
Author(s):  
Caroline N. Herndon ◽  
William J. Foreyt ◽  
Subramaniam Srikumaran
Keyword(s):  
Differential Expression ◽  
Lung Tissue ◽  
Related Species ◽  
Polymorphonuclear Leukocytes ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Interleukin 8 ◽  
Mannheimia Haemolytica ◽  
Domestic Sheep ◽  
Experimental Conditions

ABSTRACTThe pneumonic lesions and mortality caused byMannheimia haemolyticain bighorn sheep (BHS;Ovis canadensis) are more severe than those in the related species, domestic sheep (DS;Ovis aries), under both natural and experimental conditions. Leukotoxin (Lkt) and lipopolysaccharide (LPS) are the most important virulence factors of this organism. One hallmark of pathogenesis of pneumonia is the influx of polymorphonuclear leukocytes (PMNs) into the lungs. Lkt-induced cytolysis of PMNs results in the release of cytotoxic compounds capable of damaging lung tissue. Interleukin-8 (IL-8) is a potent PMN chemoattractant. The objective of the present study was to determine if there is differential expression of IL-8 by the macrophages and PMNs of BHS and DS in response toM. haemolytica. Macrophages and PMNs of BHS and DS were stimulated with heat-killedM. haemolyticaor LPS. IL-8 expression by the cells was measured by enzyme-linked immunosorbent assays and real-time reverse transcription-PCR (RT-PCR). The PMNs of BHS expressed severalfold higher levels of IL-8 than those of DS upon stimulation. Lesional lung tissue ofM. haemolytica-infected BHS contained significantly higher levels of IL-8 than nonlesional tissue. The bronchoalveolar lavage (BAL) fluid of infected BHS also contained higher levels of IL-8 than that of infected DS. Depletion of IL-8 reduced migration of PMNs toward BAL fluid by approximately 50%, indicating that IL-8 is integral to PMN recruitment to the lung duringM. haemolyticainfection. Excessive production of IL-8, enhanced recruitment of PMNs, and PMN lysis by Lkt are likely responsible for the severity of the lung lesions inM. haemolytica-infected BHS.

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