Molecular cloning, characterization and in vitro expression of SERPIN B1 of bighorn sheep (Ovis canadensis) and domestic sheep (Ovis aries), and comparison with that of other species

2010 ◽  
Vol 137 (3-4) ◽  
pp. 327-331 ◽  
Author(s):  
Renuka Subramaniam ◽  
Rohana P. Dassanayake ◽  
Junzo Norimine ◽  
Wendy C. Brown ◽  
Donald P. Knowles ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
In Vitro Expression ◽  
Serpin B1

Identification of novel variants of interferon-tau gene in Bighorn sheep (Ovis canadensis mexicana), Pelibuey sheep (Ovis aries) and its expression in hybrid blastocysts (Ovis canadensis × Ovis aries)

2012 ◽  
Vol 92 (3) ◽  
pp. 275-283 ◽  
Author(s):  
M. Palma-Irizarry ◽  
P. Damian-Matsumura ◽  
M. Betancourt ◽  
S. Romo ◽  
G. Perales ◽  
...  
Keyword(s):  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Interferon Tau ◽  
Pelibuey Sheep ◽  
Novel Variants ◽  
Ovis Canadensis Mexicana

Palma-Irizarry, M., Damian-Matsumura, P., Betancourt, M., Romo, S., Perales, G., Lagunas-Martínez, A., Madrid-Marina, M. V., Mejía, O. and Ducolomb, Y. 2012. Identification of novel variants of interferon-tau gene in Bighorn sheep ( Ovis canadensis mexicana ), Pelibuey sheep ( Ovis aries ) and its expression in hybrid blastocysts ( Ovis canadensis×Ovis aries ). Can. J. Anim. Sci. 92: 275–283. The major signal for maternal recognition of pregnancy (MRP) in ruminants is interferon-tau (IFNT). Four novel sequences of the IFNT gene were isolated from genomic DNA obtained from blood samples of Bighorn, Pelibuey or the hybrid ewes. The deduced amino acid sequence of the hybrid ewe showed 100% identity with one Bighorn sheep IFNT sequence, suggesting that this sequence was inherited from Bighorn sheep. The high degree of identity between Pelibuey and Bighorn sheep sequences could be a possible explanation for the successful MRP in some Bighorn hybrid pregnancies. In vivo and in vitro domestic sheep (Ovis aries) and hybrid sheep (Ovis aries×Ovis canadensis) blastocysts were obtained, and IFNT expression was measured by semiquantitative RT-PCR. In vivo and in vitro hybrid blastocysts showed lower expression of IFNT when compared with domestic blastocysts. In conclusion this study showed that there exists an effect of hybridization on IFNT expression and this can affect the MRP.

scholarly journals Differences in leukocyte differentiation molecule abundances on domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) neutrophils identified by flow cytometry

2016 ◽  
Vol 46 ◽  
pp. 40-46 ◽  
Author(s):  
Margaret A. Highland ◽  
David A. Schneider ◽  
Stephen N. White ◽  
Sally A. Madsen-Bouterse ◽  
Donald P. Knowles ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Domestic Sheep

scholarly journals Survival of Bighorn Sheep (Ovis canadensis) Commingled with Domestic Sheep (Ovis aries) in the Absence of Mycoplasma ovipneumoniae

2012 ◽  
Vol 48 (1) ◽  
pp. 168-172 ◽  
Author(s):  
Thomas E. Besser ◽  
E. Frances Cassirer ◽  
Catherine Yamada ◽  
Kathleen A. Potter ◽  
Caroline Herndon ◽  
...  
Keyword(s):  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Mycoplasma Ovipneumoniae

130 In Vitro Production of Hybrid Desert Bighorn×Domestic Sheep Embryos Using Frozen–Thawed Epididymal Semen from a Hunter-Harvested Ram

2018 ◽  
Vol 30 (1) ◽  
pp. 205
Author(s):  
M. G. Licea ◽  
J. E. H. Pichardo ◽  
J. L. Rodríguez ◽  
A. García-Contreras ◽  
B. C. Rosales ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Membrane Integrity ◽  
Bighorn Sheep ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Epididymal Sperm ◽  
In Vitro Production ◽  
Desert Bighorn Sheep ◽  
Vitro Production

Although considered a species of least concern by the International Union for Conservation of Nature red list, the Desert Bighorn sheep (Ovis canadensis nelsoni) is listed in Appendix II of CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora) in Mexico, due to population size and the lack of protected areas. Postmortem epididymal sperm collected from a hunter-harvested Desert Bighorn sheep ram in Mexico, with an unofficial Safari Club International score of 197 2/8 and an estimated 5.5 years old, were used to evaluate the in vitro production (IVP) of embryos using postmortem-collected ram sperm. Testicles with epididymides were placed in the refrigerator ~45 min after harvest. Sperm were extracted from each epididymis and assessed separately for total motility (TM), progressive motility (PM), and membrane integrity using a phase contrast microscope. The sperm suspension was obtained from the distal end of both epididymides and cryopreserved 12 h postmortem using triladyl with egg yolk. Membrane integrity and morphology were evaluated using Eosin-Nigrosin stain. Sperm DNA fragmentation was analysed using the Halomax kit (Halosperm SL, Madrid, Spain) with fluorescence microscopy. Centrifugation with density gradient PureSperm (Nidacon International, Mölndal, Sweden) was used to remove dead sperm and debris before IVF. Ovaries were collected from Domestic sheep (Ovis aries) at a local slaughterhouse. The maturation medium was TCM-199 with Earle’s salts and a modified Tris-buffered medium was used for fertilization. Frozen straws of sperm from the Desert Bighorn ram were thawed for 45 s at 37°C. Sperm were diluted with modified Tween medium B with milk powder (mTBM) to a final concentration of 5 × 106 cells mL−1. The gametes were co-incubated for 18 h under previously described conditions. The cumulus cells were mechanically removed from zygotes and grown using a co-culture with granulosa cells in sequential media SOF1-SOF2. With regard to sperm collection, epididymis 1 produced 29 straws of sperm (0.25 mL, 136 × 106 sperm mL−1) and epididymis 2 produced 32 straws of sperm (0.25 mL, 68 × 106 sperm mL−1). The sperm sample used for IVF had TM of 60% and PM of 30%. Live dead staining of fresh sperm showed 68% live (i.e. intact cell membranes) and 28% post-thaw. Regarding DNA integrity, only 2% of sperm had DNA fragmentation at 0 h. Of 15 Grade 1 oocytes used for IVF, 4 cleaved (27%), with 1 developing to blastocyst stage (25%). The results show that frozen–thawed epididymal sperm collected from a recently deceased Desert Bighorn ram can provide a valuable source of sperm for IVP of embryos. These results also provide new information on Desert Bighorn sheep reproductive parameters for use in health assessment, or reproduction and conservation management through gene banking and assisted reproductive techniques.

scholarly journals Shared Bacterial and Viral Respiratory Agents in Bighorn Sheep (Ovis canadensis), Domestic Sheep (Ovis aries), and Goats (Capra hircus) in Montana

2011 ◽  
Vol 2011 ◽  
pp. 1-12 ◽  
Author(s):  
David S. Miller ◽  
Glen C. Weiser ◽  
Keith Aune ◽  
Brent Roeder ◽  
Mark Atkinson ◽  
...  
Keyword(s):  
Disease Outbreaks ◽  
Bighorn Sheep ◽  
Cross Sectional Study ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Capra Hircus ◽  
Domestic Sheep ◽  
Infectious Agents ◽  
Cross Sectional ◽  
The Impact

Transmission of infectious agents from livestock reservoirs has been hypothesized to cause respiratory disease outbreaks in bighorn sheep (Ovis canadensis), and land management policies intended to limit this transmission have proven controversial. This cross-sectional study compares the infectious agents present in multiple populations of bighorn sheep near to and distant from their interface with domestic sheep (O. aries) and domestic goat (Capra hircus) and provides critical baseline information needed for interpretations of cross-species transmission risks. Bighorn sheep and livestock shared exposure to Pasteurellaceae, viral, and endoparasite agents. In contrast, although the impact is uncertain,Mycoplasmasp. was isolated from livestock but not bighorn sheep. These results may be the result of historic cross-species transmission of agents that has resulted in a mosaic of endemic and exotic agents. Future work using longitudinal and multiple population comparisons is needed to rigorously establish the risk of outbreaks from cross-species transmission of infectious agents.

scholarly journals Differential pulmonary immunopathologic response of domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) to Mycoplasma ovipneumoniae infection: a retrospective study

2019 ◽  
Author(s):  
Paige C. Grossman ◽  
David A. Schneider ◽  
Donald P. Knowles ◽  
Margaret A. Highland
Keyword(s):  
Retrospective Study ◽  
Lung Tissue ◽  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Respiratory Pathogen ◽  
Domestic Sheep ◽  
Lung Sample ◽  
Ffpe Tissues ◽  
Mycoplasma Ovipneumoniae

AbstractMycoplasma ovipneumoniae is a respiratory pathogen that can impact domestic sheep (Ovis aries; DS) and bighorn sheep (Ovis canadensis; BHS). Experimental and field data have indicated BHS are more susceptible than DS to developing polymicrobial pneumonia associated with Mycoplasma ovipneumoniae infection. We hypothesized that DS and BHS have a differential immunopathologic pulmonary response to M. ovipneumoniae infection. A retrospective study was performed using formalin-fixed, paraffin-embedded (FFPE) lung tissue from DS and BHS without and with M. ovipneumoniae detected in the lung tissue (n=8 per group). While each M. ovipneumoniae positive lung sample had microscopic changes typical of infection, including hyperplasia of intrapulmonary bronchus-associated lymphoid tissue (BALT) and respiratory epithelium, DS exhibited a more robust and well-organized BALT formation as compared to BHS. Immunohistochemistry was performed with antibodies reactive in FFPE tissues and specific for leukocyte and cytokine markers: T cell marker CD3, B cell markers CD20 and CD79a, macrophage markers CD163 and Iba1, and cytokine IL-17. Digital analysis was used to quantitate chromogen deposition in regions of interest (ROIs), including alveolar and bronchiolar areas, and bronchiolar subregions (epithelium and BALT). Main effects and interaction of species and infection status were analyzed by beta regression and Bonferroni corrections were performed on pairwise comparisons (PBon<0.05 significance). Significant species differences were identified for bronchiolar CD3 (PBon=0.0023) and CD163 (PBon=0.0224), alveolar CD163 (PBon=0.0057), and for IL-17 in each of the ROIs (alveolar: PBon=0.0009; BALT: PBon=0.0083; epithelium: PBon=0.0007). Infected BHS had a higher abundance of bronchiolar CD3 (PBon=0.0005) and CD163 (PBon=0.0162), and alveolar CD163 (PBon=0.0073). While IL-17 significantly increased with infection in BHS BALT (PBon=0.0179) and alveolar (0.0006) ROIs, abundance in DS showed an insignificant decrease in these ROIs and a significant decrease in epithelial abundance (PBon=0.0019). These findings support the hypothesis that DS and BHS have a differential immunopathologic response to M. ovipneumoniae infection.

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