scholarly journals The EO771 mammary cancer cell line displays a luminal B phenotype and it is sensitive to anti-estrogen treatments

2019 ◽  
Author(s):  
Augustin Le Naour ◽  
Yvonne Koffi ◽  
Mariane Diab ◽  
Delphine Le Guennec ◽  
Stéphanie Rougé ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Cell Line ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Cancer Cell Line ◽  
Molecular Classification ◽  
Luminal B ◽  
Mammary Cancer Cell ◽  
Mammary Cancer Cell Line

ABSTRACTDespite decades of therapeutic trials, detection, many drugs available and numerous studies on breast cancer, it remains the most deadly cancer in women. In order to choose the most appropriate treatment and to know the prognosis of the patients, the breast cancer is divided into different subtypes using a molecular classification. Thus, the need to discover new effective therapy is necessary, requiring to have models to test them. The EO771 (also named E0771 or EO 771) murine mammary cancer cell line is originally isolated from a spontaneous tumor in C57BL/6 mouse. Although frequently used, this cell line remains poorly characterized. Therefore, the EO771 phenotype was investigated. Transcriptomic and protein analysis allowed to classify the EO771 as luminal B subtype and more precisely estrogen receptor α negative, estrogen receptor β positive, progesterone receptor positive and ErbB2 positive. This phenotype was associated to a sensitivity to anti-estrogen treatments such as tamoxifen, 4-hydroxy-tamoxifen, endoxifen and fulvestrant.

scholarly journals EO771, the first luminal B mammary cancer cell line from C57BL/6 mice

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Augustin Le Naour ◽  
Yvonne Koffi ◽  
Mariane Diab ◽  
Delphine Le Guennec ◽  
Stéphanie Rougé ◽  
...  
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Cancer Cell Line ◽  
Luminal B ◽  
Mammary Cancer Cell ◽  
Mammary Cancer Cell Line

scholarly journals Modulation of C5a-C5aR interactions against murine mammary cancer cell line

2021 ◽  
Author(s):  
Kamarudin N. H. ◽  
Bachek N. F. ◽  
Mohd Hezmee Mohd Noor
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Cancer Cell Line ◽  
Mammary Cancer Cell ◽  
Mammary Cancer Cell Line

Biological responses of tamoxifen in the fetal and newborn vagina and uterus of the guinea-pig and in the R-27 mammary cancer cell line

1986 ◽  
Vol 24 (1) ◽  
pp. 99-108 ◽  
Author(s):  
J.R. Pasqualini ◽  
N. Giambiagi ◽  
C. Sumida ◽  
B.-L. Nguyen ◽  
C. Gelly ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Cell Line ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Cancer Cell Line ◽  
Mammary Cancer Cell ◽  
Biological Responses ◽  
Mammary Cancer Cell Line

Growth inhibitory effect of 4-hydroxy-tamoxifen on the BT-20 mammary cancer cell line

1988 ◽  
Vol 31 (4) ◽  
pp. 655-663 ◽  
Author(s):  
C. Chouvet ◽  
E. Vicard ◽  
L. Frappart ◽  
N. Falette ◽  
M.F. Lefebvre ◽  
...  
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Cancer Cell Line ◽  
Inhibitory Effect ◽  
Growth Inhibitory Effect ◽  
Mammary Cancer Cell ◽  
Growth Inhibitory ◽  
Mammary Cancer Cell Line

Characterization of Non-Adherent Cells from IPC-366, A Canine Inflammatory Mammary Cancer Cell Line

2017 ◽  
Vol 156 (1) ◽  
pp. 120
Author(s):  
S. Caceres ◽  
L. Peña ◽  
L. Lacerda ◽  
M.J. Illera ◽  
R.A. Larson ◽  
...  
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Cancer Cell Line ◽  
Adherent Cells ◽  
Mammary Cancer Cell ◽  
Mammary Cancer Cell Line

scholarly journals Abstract 1161: Interaction between doxorubicin and amniotic membrane stem cells on the canine inflammatory mammary cancer cell line IPC-366

2018 ◽  
Author(s):  
Jessica Borghesi ◽  
Sara Caceres ◽  
Lara C. Mario ◽  
Rafael Gonçalves Hayashi ◽  
Laura Peña ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Amniotic Membrane ◽  
Mammary Cancer ◽  
Cancer Cell Line ◽  
Mammary Cancer Cell ◽  
Mammary Cancer Cell Line

Melatonin Treatment Combined with TGF-β Silencing Inhibits Epithelial- Mesenchymal Transition in CF41 Canine Mammary Cancer Cell Line

2020 ◽  
Vol 20 (8) ◽  
pp. 989-997
Author(s):  
Paulo R. Custódio ◽  
Jucimara Colombo ◽  
Fabrício V. Ventura ◽  
Tialfi B. Castro ◽  
Debora A.P.C. Zuccari
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Epithelial Mesenchymal Transition ◽  
Cancer Cell Line ◽  
Mesenchymal Transition ◽  
Mammary Cancer Cell ◽  
Canine Mammary Cancer ◽  
Mammary Cancer Cell Line ◽  
Tgf Β1

Background: Mammary cancer is the most prevalent type of cancer in female dogs. The main cause of mortality is the occurrence of metastasis. The metastatic process is complex and involves the Epithelial- Mesenchymal Transition (EMT), which can be activated by Transforming Growth Factor beta (TGF-β) and involves changes in cellular phenotype, as well as, in the expression of proteins such as E-cadherin, N-cadherin, vimentin and claudin-7. Melatonin is a hormone with oncostatic and anti-metastatic properties and appears to participate in the TGF-β pathway. Thus, the present work aimed to evaluate the expression of EMT markers, E-cadherin, N-cadherin, vimentin and claudin-7, as well as, the cell migration of the canine mammary cancer cell line, CF41, after treatment with melatonin and TGF-β silencing. Methods: Canine mammary cancer cell line, CF41, was cultured and characterized in relation to markers ER, PR and HER2. Cell line CF41 with reducing expression level of TGF-βwas performed according to Leonel et al. (2017). Expression of the protein E-caderin, N-cadherin, vimentin and claudin-7 was evaluated by immunocytochemistry and quantified by optical densitometry. The analysis of cell migration was performed in transwell chambers with 8μM pore size membrane. Results: CF41 cells present a triple negative phenotype, which is an aggressive phenotype. Immunocytochemistry staining showed increased expression of E-caderin and claudin-7 (P˂0.05) and decreased expression of N-cadherin and vimentin (P˂0.05) in CF41 cells after treatment with 1mM melatonin and TGF-β silencing. Moreover, treatment with melatonin and TGF-β silencing was able to reduce migration in cell line CF41 (P˂0.05). Conclusion: Our data suggests that therapies combining TGF- β1 silencing and melatonin may be effective in suppressing the process of EMT, corroborating the hypothesis that melatonin acts on the TGF-β1 pathway and can reduce the metastatic potential of CF41 cells. This is so far the first study that reports melatonin treatment in CF41 cells with TGF-β1 silencing and its effect on EMT. Thus, further studies are needed to confirm this hypothesis.

scholarly journals Establishment and characterization of a new feline mammary cancer cell line, FkMTp

2016 ◽  
Vol 68 (4) ◽  
pp. 1529-1543 ◽  
Author(s):  
Ana Borges ◽  
Filomena Adega ◽  
Raquel Chaves
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Cancer Cell Line ◽  
Mammary Cancer Cell ◽  
Mammary Cancer Cell Line
Sign in / Sign up

Export Citation Format

Share Document