scholarly journals Genome sequencing and neurotoxin diversity of a wandering spider Pardosa pseudoannulata (pond wolf spider)

2019 ◽  
Author(s):  
Na Yu ◽  
Jingjing Li ◽  
Meng Liu ◽  
Lixin Huang ◽  
Haibo Bao ◽  
...  
Keyword(s):  
Pest Control ◽  
Potential Role ◽  
Insect Pests ◽  
Wolf Spider ◽  
Spider Species ◽  
Pardosa Pseudoannulata ◽  
Genome Data ◽  
Silk Glands ◽  
Important Predator ◽  
High Quality Genome

AbstractSpiders constitute an extensive and diverse branch of the phylum Arthropoda. Whereas the genomes of four web-weaver spider species and a single cave-living spider have been determined, similar studies have not been reported previously for a wandering spider. The pond wolf spider, Pardosa pseudoannulata, is a wandering hunter that immobilizes prey using venom rather than a web. It is also an important predator against a range of agriculturally important insect pests. The increasing interest in its wandering lifestyle and in the potential of spider venom as a tool for pest control have prompted a detailed study on this wandering spider species. We have generated a high-quality genome sequence of P. pseudoannulata and analysed genes associated with the production of silk and venom toxins. Sequencing reveals that P. pseudoannulata has a large genome of 4.26 Gb. The presence of only 16 spidroin genes and four types of silk glands is consistent with the moderate use of silk and the lack of a prey-catching web. A large number of genes encode neurotoxins and there is evidence that the majority are highly selective for invertebrates. Comparison between spider species reveals a correlation between spider neurotoxin selectivity for target organisms and spider prosoma size, suggesting a possible coevolution of these two features. The genome data provides valuable insights into the biology of P. pseudoannulata and its potential role as a natural enemy in pest control.

scholarly journals KELIMPAHAN DAN KEANEKARAGAMAN SPESIES LABA-LABA PREDATOR HAMA PADI RATUN DI SAWAH PASANG SURUT

2014 ◽  
Vol 14 (1) ◽  
pp. 1-7
Author(s):  
Siti Herlinda ◽  
Hendri Candro Nauli Manalu ◽  
Rinda Fajrin Aldina ◽  
Suwandi Suwandi ◽  
Andi Wijaya ◽  
...  
Keyword(s):  
Species Diversity ◽  
Insect Pests ◽  
Species Number ◽  
Spider Species ◽  
Pardosa Pseudoannulata ◽  
Pests And Diseases ◽  
Rice Pests ◽  
Main Crop ◽  
Number Species ◽  
Paddy Area

ABSTRACTAbundance and Species Diversity of Predatory Spiders for Insect Pests Inhabiting Ratoon Paddy in Tidal Lowland.  Ratoon paddy productivity is lower than the main crop.  Increasing productivity of the ratoon paddy is to protect the paddy from pests and diseases. This study aimed to analyze the abundance and species diversity of predatory spiders of rice pests on ratoon paddy in tidal lowland. Ratoon paddy field observed was from the main crop that has been harvested and cut 20 cm height. Paddy area observed was 2 ha field planted using Ciherang and Inpara varieties. Spiders inhabiting canopy were sampled using insect nets but soil dwelling spiders were trapped using pitfall traps. The result showed that spider species inhabiting canopy of ratoon paddy were Pardosa pseudoannulata, Lycosa chaperi, Araneus inustus, Cylosa insulana, Atypena adelinae, Erigone bifurca, Erigonidium graminicola, Oxyopes javanus, Argyrodes miniaceus, and Marpisa magister.  Spider species found soil dwelling were 11 species, namely Pardosa pseudoannulata, Pardosa sumatrana, Pardosa mackenziei, Pardosa oakleyi, Hogna rizali, Araneus inustus, Cylosa insulana, T. vermiformis, Runcinia albostriata, Coleosoma octomaculatum, and Marpisa magister. Abundance, species number, species diversity for soil dwelling spiders were higher than those for canopy inhabiting spiders. However, the abundance, species number, species diversity for the soil dwelling spiders  tended to be higher on the ratoon paddy 3, 4, and 5 week old than those on 1, 2, and 6 week old.  From the result, we concluded that abundance, species number, species diversity of spiders found in the ratoon paddy were higher or tended similar to those on the main crop.

scholarly journals Infection and transmission of the facultative endosymbiont Arsenophonus in the spider Pardosa pseudoannulata

2020 ◽  
Author(s):  
Hanqi Jiang ◽  
Yu Ding ◽  
Dongxiao Zhao ◽  
Xiangdong Liu ◽  
Huifang Guo
Keyword(s):  
Genetic Differentiation ◽  
Insect Pests ◽  
Transmission Rate ◽  
The Other ◽  
Diagnostic Pcr ◽  
Pardosa Pseudoannulata ◽  
Rice Planthoppers ◽  
Geographical Regions ◽  
Important Predator ◽  
Average Transmission

Abstract Background The infection of insect pests and their parasitoids with the facultative endosymbiont Arsenophonus has been well studied, whereas the infection of the predators of insect pests with this symbiont is less well understood. We studied this symbiont in the spider Pardosa pseudoannulata, an important predator of rice planthoppers, collected from rice paddies in 8 geographical regions in China.Results Diagnostic PCR showed that Arsenophonus was present in 4 of the 8 sampled populations of spiders. In these 4 populations, the infection incidence was between 2.1% and 16.7% and significantly differed between the Lishui population and the other three populations. Investigation of symbiont infection in both females and their offspring indicated that Arsenophonus can be vertically transmitted in P. pseudoannulata; the average transmission rate was approximately 25%. Remarkably, Arsenophonus was present in approximately 11% of the offspring of uninfected females, indicating that nonmaternal transmission occurs in this spider.Conclusions Populations of the spider P. pseudoannulata are infected at low rates with the symbiont Arsenophonus. This symbiont can be vertically transmitted from female spiders to offspring but may also be transmitted by male spiders or other vectors. Arsenophonus populations from different geographical regions exhibit genetic differentiation.

Regulatory roles of microRNAs in insect pests: prospective targets for insect pest control

2021 ◽  
Vol 70 ◽  
pp. 158-166
Author(s):  
Qiang Zhang ◽  
Wei Dou ◽  
Clauvis Nji Tizi Taning ◽  
Guy Smagghe ◽  
Jin-Jun Wang
Keyword(s):  
Pest Control ◽  
Insect Pests ◽  
Insect Pest ◽  
Insect Pest Control

Characterization of cadmium-responsive transcription factors in wolf spider Pardosa pseudoannulata

Chemosphere ◽  
2021 ◽  
Vol 268 ◽  
pp. 129239
Author(s):  
Juan Wang ◽  
Yuan He ◽  
Xianjin Peng ◽  
Bo lv ◽  
Zhi Wang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Wolf Spider ◽  
Pardosa Pseudoannulata

scholarly journals An unusual new wolf spider species from the Erg Chebbi Desert in Morocco (Araneae: Lycosidae: Evippinae)

2017 ◽  
Vol 45 (3) ◽  
pp. 344-355 ◽  
Author(s):  
Steffen Bayer ◽  
Rainer Foelix ◽  
Mark Alderweireldt
Keyword(s):  
Wolf Spider ◽  
Spider Species

scholarly journals Konsep dan Implementasi Teknologi Budi Daya Ramah Lingkungan pada Tanaman Tembakau, Serat, dan Minyak Industri Concept and Implementation of Environmentally-Friendly Technologies in Cultivation of Tobacco, Fiber, and Industrial Oil Crops

2016 ◽  
Vol 1 (1) ◽  
pp. 41
Author(s):  
. Nurindah
Keyword(s):  
Pest Control ◽  
Insect Pests ◽  
Environmentally Friendly ◽  
Land Conservation ◽  
Technology Innovations ◽  
Industrial Oil ◽  
Oil Crops ◽  
Chemical Pesticides

<p>Penerapan teknologi ramah lingkungan budi daya tanaman pada suatu lahan akan dapat mempertahankan kelestarian lingkungan. Penciptaan teknologi budi daya tanaman tembakau, serat, dan minyak industri di-arahkan pada teknologi yang dapat meningkatkan produktivitas dan mutu hasil, efisiensi biaya usaha tani, dan ramah lingkungan. Teknologi ramah lingkungan difokuskan pada penemuan komponen teknologi prapa-nen yang mempunyai dampak minimal terhadap pencemaran atau perusakan lingkungan, yang meliputi va-rietas-varietas unggul, teknik pengendalian hama dan penyakit, teknik konservasi lahan tembakau. Varietas-varietas unggul tersebut adalah varietas-varietas yang mempunyai ketahanan terhadap hama dan penyakit, yaitu tembakau Prancak 95, Prancak N1, Prancak N2, Kemloko 2, dan Grompol Jatim 1; kapas: Kanesia 11–Kanesia 13; kenaf: Karangploso 14–Karangploso 15; wijen: Sumberrejo 4; dan jarak kepyar: Asembagus 81. Teknik pengendalian hama dan penyakit yang ramah lingkungan adalah teknologi pengendalian hama yang membatasi atau meniadakan penggunaan insektisida kimia sintetik dan menerapkan teknik pengendalian de-ngan memanfaatkan peran musuh alami serangga hama atau antagonis patogen penyebab penyakit, dan penggunaan pestisida nabati. Teknik konservasi lahan untuk mengendalikan erosi dan penyakit lincat dikem-bangkan pada lahan tembakau temanggung dengan menerapkan penggunaan varietas tahan penyakit, pem-buatan terassering dan penguatnya, pengolahan lahan minimal, dan aplikasi mikroba antagonis. Teknologi ramah lingkungan tersebut telah diterapkan di tingkat petani dan memberikan dampak yang positif terhadap pengembangan komoditas.</p><p>Technology innovations for tobacco, fibers, and industrial-oil crops are directed to increase production and quality of the products, efficiency, and environmentally-friendly technologies. The efficiency and environ-menttally-friendly technologies are focused on the pre-harvest technology innovations that have minimal im-pacts on environmental damages. The technologies include superior varieties, pest control, and land conser-vation. The superior varieties are those that resistant to either insect pests or diseases, i.e. tobacco: Prancak 95, Prancak N1, Prancak N2, Kemloko 2, and Grompol Jatim 1; cotton: Kanesia 11–Kanesia 13; kenaf: Ka-rangploso 14–Karangploso 15; sesame: Sumberrejo 4; and  castor: Asembagus 81.  Environmentally-friendly pest control is to limit or no use synthetic-chemical pesticides in pest control, but optimally make use the role of natural enemies and antagonists and use biopesticides. Land conservation technique to control erosi-on as well as ”lincat’ disease has been developed in fields of temanggung tobacco by using tobacco variety resistant to the disease, terracering, minimum tillage, and application of antagonist microbes. Those techno-logies has been implemented in the farmers’ fields and has a positive impacts for the commodity develop-ment.</p><p> </p>

scholarly journals Evaluation of New Systemic Insecticides for Elm Insect Pest Control

1996 ◽  
Vol 14 (1) ◽  
pp. 22-26 ◽  
Author(s):  
D. Casey Sclar ◽  
Whitney S. Cranshaw
Keyword(s):  
Pest Control ◽  
Woody Plants ◽  
Insect Pests ◽  
Insect Pest ◽  
Leaf Beetle ◽  
Pest Species ◽  
Shade Trees ◽  
Insect Pest Control ◽  
Systemic Activity ◽  
Systemic Insecticides

Abstract Use of systemic insecticides that can be injected either into the root system or trunk of woody plants provides several potential advantages, notably in control of drift during application. Recently, new classes of insecticides with systemic activity have been developed, which may supplant the organophosphate and carbamate systemic insecticides that have previously been available. To evaluate their potential to control insects affecting shade trees, studies were conducted using imidacloprid and abamectin on elm. Soil injections of imidacloprid appeared particularly effective, controlling all three of the target pest species in this study (elm leaf beetle, European elm scale, elm leaf aphid). Both imidacloprid and abamectin also were effective against at least some elm insects when injected into trunks. Persistence of irnidacloprid was unusually long, providing second season control of all elm insect pests, although root uptake following soil injections was slow.

scholarly journals Pesticide application has little influence on coding and non-coding gene expressions in rice

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Sajid Muhammad ◽  
Jingai Tan ◽  
Pingchuan Deng ◽  
Tingting Li ◽  
Haohua He ◽  
...  
Keyword(s):  
Potential Role ◽  
Insect Pests ◽  
Pesticide Application ◽  
Gene Expressions ◽  
Number Of Genes ◽  
Non Coding Rnas ◽  
Transcriptomic Changes ◽  
Harmful Effects ◽  
Host Genes

Abstract Background Agricultural insects are one of the major threats to crop yield. It is a known fact that pesticide application is an extensive approach to eliminate insect pests, and has severe adverse effects on environment and ecosystem; however, there is lack of knowledge whether it could influence the physiology and metabolic processes in plants. Results Here, we systemically analyzed the transcriptomic changes in rice after a spray of two commercial pesticides, Abamectin (ABM) and Thiamethoxam (TXM). We found only a limited number of genes (0.91%) and (1.24%) were altered by ABM and TXM respectively, indicating that these pesticides cannot dramatically affect the performance of rice. Nevertheless, we characterized 1140 Differentially Expressed Genes (DEGs) interacting with 105 long non-coding RNAs (lncRNAs) that can be impacted by the two pesticides, suggesting their certain involvement in response to farm chemicals. Moreover, we detected 274 alternative splicing (AS) alterations accompanied by host genes expressions, elucidating a potential role of AS in control of gene transcription during insecticide spraying. Finally, we identified 488 transposons that were significantly changed with pesticides treatment, leading to a variation in adjacent coding or non-coding transcripts. Conclusion Altogether, our results provide valuable insights into pest management through appropriate timing and balanced mixture, these pesticides have no harmful effects on crop physiology over sustainable application of field drugs.

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