scholarly journals Theories and Applications for Sequencing Randomly Selected Clones

2001 ◽  
Vol 11 (2) ◽  
pp. 274-280
Author(s):  
Michael C. Wendl ◽  
Marco A. Marra ◽  
LaDeana W. Hillier ◽  
Asif T. Chinwalla ◽  
Richard K. Wilson ◽  
...  
Keyword(s):  
Human Chromosome ◽  
The Other ◽  
Chromosome 2 ◽  
Contig Assembly ◽  
Secondary Importance ◽  
Clone Size ◽  
Bac Clones ◽  
Clone Sequencing ◽  
Order Of Magnitude ◽  
Random Approach

Theory is developed for the process of sequencing randomly selected large-insert clones. Genome size, library depth, clone size, and clone distribution are considered relevant properties and perfect overlap detection for contig assembly is assumed. Genome-specific and nonrandom effects are neglected. Order of magnitude analysis indicates library depth is of secondary importance compared to the other variables, especially as clone size diminishes. In such cases, the well-known Poisson coverage law is a good approximation. Parameters derived from these models are used to examine performance for the specific case of sequencing random human BAC clones. We compare coverage and redundancy rates for libraries possessing uniform and nonuniform clone distributions. Results are measured against data from map-based human-chromosome-2 sequencing. We conclude that the map-based approach outperforms random clone sequencing, except early in a project. However, simultaneous use of both strategies can be beneficial if a performance-based estimate for halting random clone sequencing is made. Results further show that the random approach yields maximum effectiveness using nonbiased rather than biased libraries.

Sialoadhesin (Sn) Maps to Mouse Chromosome 2 and Human Chromosome 20 and Is Not Linked to the Other Members of the Sialoadhesin Family, CD22, MAG, and CD33

Genomics ◽  
1995 ◽  
Vol 28 (2) ◽  
pp. 344-346 ◽  
Author(s):  
Stuart Mucklow ◽  
Adele Hartnell ◽  
Marie-Geneviève Mattei ◽  
Siamon Gordon ◽  
Paul R. Crocker
Keyword(s):  
Human Chromosome ◽  
Mouse Chromosome ◽  
The Other ◽  
Chromosome 2 ◽  
Chromosome 20 ◽  
Mouse Chromosome 2

scholarly journals A COMPARISON OF HEAT AND INTERCHROMOSOMAL EFFECTS ON RECOMBINATION AND INTERFERENCE IN DROSOPHILA MELANOGASTER

Genetics ◽  
1978 ◽  
Vol 89 (1) ◽  
pp. 65-77
Author(s):  
R F Grell
Keyword(s):  
Drosophila Melanogaster ◽  
X Chromosome ◽  
Thermal Dissociation ◽  
Temporal Sequence ◽  
The Other ◽  
Centromeric Heterochromatin ◽  
Drosophila Genome ◽  
Chromosome 2 ◽  
Independent Control ◽  
Order Of Magnitude

ABSTRACT Heat and interchromosomal effects on recombination have been compared for 23 regions comprising the predominantly euchromatic portions of the five arms of the Drosophila genome. Patterns of response are strikingly similar, with both modifiers causing proximal and distal increases and minimal effects in the middle of the arms. Changes in interference for the same regions in the presence of the two modifiers reveal little similarity, except for the X chromosome. The question of independent control of interference and recombination, as well as alternatives for their temporal sequence, is discussed. Recombination response to the two modifiers in the centric heterochromatin of chromosoaime 2 is markedly different from that found in euchromatin. The interchromosomal effect is absent here, whereas heat induces an increase roughly an order of magnitude greater than that found in euchromatin and totally unlike the lack of response in the proximal heterochromatin of the X chromosome. It is proposed that the sequestering of DNA satellite I (thermal dissociation 9-20° lower than that of the other major satellites) in the centromeric heterochromatin of chromosome 2 (but not in X or 3) may account for the increase.

Comparison of the Potency of 8-L-Arginine, 8-D-Arginine and 8-D-Homoarginine Vasopressin Analogs with Substituted Phenylalanine in Position 2

1994 ◽  
Vol 59 (6) ◽  
pp. 1439-1450 ◽  
Author(s):  
Miroslava Žertová ◽  
Jiřina Slaninová ◽  
Zdenko Procházka
Keyword(s):  
Amino Acid ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Basic Amino Acid ◽  
The Other ◽  
Side Chain ◽  
Inhibitory Potency ◽  
Phase Synthesis ◽  
Other Hand ◽  
Order Of Magnitude

An analysis of the uterotonic potencies of all analogs having substituted L- or D-tyrosine or -phenylalanine in position 2 and L-arginine, D-arginine or D-homoarginine in position 8 was made. The series of analogs already published was completed by the solid phase synthesis of ten new analogs having L- or D-Phe, L- or D-Phe(2-Et), L- or D-Phe(2,4,6-triMe) or D-Tyr(Me) in position 2 and either L- or D-arginine in position 8. All newly synthesized analogs were found to be uterotonic inhibitors. Deamination increases both the agonistic and antagonistic potency. In the case of phenylalanine analogs the change of configuration from L to D in position 2 enhances the uterotonic inhibition for more than 1 order of magnitude. The L to D change in position 8 enhances the inhibitory potency negligibly. Prolongation of the side chain of the D-basic amino acid in position 8 seems to decrease slightly the inhibitory potency if there is L-substituted amino acid in position 2. On the other hand there is a tendency to the increase of the inhibitory potency if there is D-substituted amino acid in position 2.

scholarly journals Epistatic Control of Non-Mendelian Inheritance in Mouse Interspecific Crosses

Genetics ◽  
1996 ◽  
Vol 143 (4) ◽  
pp. 1739-1752 ◽  
Author(s):  
Xavier Montagutelli ◽  
Rowena Turner ◽  
Joseph H Nadeau
Keyword(s):  
X Chromosome ◽  
Genetic Basis ◽  
Mendelian Inheritance ◽  
The Other ◽  
Interspecific Crosses ◽  
Chromosome 2 ◽  
Mus Spretus ◽  
F1 Hybrid ◽  
Strong Deviation ◽  
Marker Loci

Abstract Strong deviation of allele frequencies from Mendelian inheritance favoring Mus spretus-derived alleles has been described previously for X-linked loci in four mouse interspecific crosses. We reanalyzed data for three of these crosses focusing on the location of the gene(s) controlling deviation on the X chromosome and the genetic basis for incomplete deviation. At least two loci control deviation on the X chromosome, one near Xist (the candidate gene controlling X inactivation) and the other more centromerically located. In all three crosses, strong epistasis was found between loci near Xist and marker loci on the central portion of chromosome 2. The mechanism for this deviation from Mendelian expectations is not yet known but it is probably based on lethality of embryos carrying particular combinations of alleles rather than true segregation distortion during oogenesis in F1 hybrid females.

Human chromosome 2 rod/ring mosaicism: probable origin by prezygotic breakage and intrachromosomal exchange

1982 ◽  
Vol 33 (3) ◽  
pp. 222-231 ◽  
Author(s):  
H.E. Wyandt ◽  
R. Kasprzak ◽  
A. Lamb ◽  
K. Willson ◽  
W.G. Wilson ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Chromosome 2 ◽  
Probable Origin

Assignment of the T-cell differentiation gene MAL to human chromosome 2, region cen?q13

1988 ◽  
Vol 27 (2) ◽  
pp. 91-95 ◽  
Author(s):  
Miguel A. Alonso ◽  
David E. Barton ◽  
Uta Francke
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Human Chromosome ◽  
T Cell Differentiation ◽  
Chromosome 2 ◽  
Differentiation Gene

scholarly journals On the 2PN Periastron Precession of the Double Pulsar PSR J0737–3039A/B

Universe ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 443
Author(s):  
Lorenzo Iorio
Keyword(s):  
General Relativity ◽  
Present Author ◽  
Moment Of Inertia ◽  
The Other ◽  
Orbital Elements ◽  
Binary Pulsars ◽  
Orbital Phase ◽  
Order Of Magnitude ◽  
General Relativistic ◽  
The Moment

One of the post-Keplerian (PK) parameters determined in timing analyses of several binary pulsars is the fractional periastron advance per orbit kPK. Along with other PK parameters, it is used in testing general relativity once it is translated into the periastron precession ω˙PK. It was recently remarked that the periastron ω of PSR J0737–3039A/B may be used to measure/constrain the moment of inertia of A through the extraction of the general relativistic Lense–Thirring precession ω˙LT,A≃−0.00060∘yr−1 from the experimentally determined periastron rate ω˙obs provided that the other post-Newtonian (PN) contributions to ω˙exp can be accurately modeled. Among them, the 2PN seems to be of the same order of magnitude of ω˙LT,A. An analytical expression of the total 2PN periastron precession ω˙2PN in terms of the osculating Keplerian orbital elements, valid not only for binary pulsars, is provided, thereby elucidating the subtleties implied in correctly calculating it from k1PN+k2PN and correcting some past errors by the present author. The formula for ω˙2PN is demonstrated to be equivalent to that obtainable from k1PN+k2PN by Damour and Schäfer expressed in the Damour–Deruelle (DD) parameterization. ω˙2PN actually depends on the initial orbital phase, hidden in the DD picture, so that −0.00080∘yr−1≤ω˙2PN≤−0.00045∘yr−1. A recently released prediction of ω˙2PN for PSR J0737–3039A/B is discussed.

scholarly journals Dok1Encoding p62dokMaps to Mouse Chromosome 6 and Human Chromosome 2 in a Region of Translocation in Chronic Lymphocytic Leukemia

Genomics ◽  
1998 ◽  
Vol 53 (2) ◽  
pp. 243-245 ◽  
Author(s):  
Keats Nelms ◽  
Andrew J. Snow ◽  
Konrad Noben-Trauth
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Human Chromosome ◽  
Mouse Chromosome ◽  
Lymphocytic Leukemia ◽  
Chromosome 6 ◽  
Chromosome 2
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