scholarly journals Sequence Comparison of Human and Mouse Genes Reveals a Homologous Block Structure in the Promoter Regions

2004 ◽  
Vol 14 (9) ◽  
pp. 1711-1718 ◽  
Author(s):  
Y. Suzuki
Keyword(s):  
Sequence Comparison ◽  
Block Structure ◽  
Promoter Regions ◽  
Human And Mouse

DNA sequence comparison of human and mouse retinitis pigmentosa GTPase regulator (RPGR) identifies tissue-specific exons and putative regulatory elements

2001 ◽  
Vol 109 (3) ◽  
pp. 271-278 ◽  
Author(s):  
Renate Kirschner ◽  
Deniz Erturk ◽  
Christina Zeitz ◽  
Selen Sahin ◽  
Juliane Ramser ◽  
...  
Keyword(s):  
Retinitis Pigmentosa ◽  
Dna Sequence ◽  
Sequence Comparison ◽  
Regulatory Elements ◽  
Tissue Specific ◽  
Retinitis Pigmentosa Gtpase Regulator ◽  
Human And Mouse ◽  
Dna Sequence Comparison

Molecular Cloning, Characterization and Expression of cDNA for Rabbit Brain Tissue Factor

1991 ◽  
Vol 66 (03) ◽  
pp. 315-320 ◽  
Author(s):  
Aruna Pawashe ◽  
Michael Ezekowitz ◽  
Tsung-Chung Lin ◽  
Renata Horton ◽  
Ronald Bach ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Sequence Comparison ◽  
Plaque Rupture ◽  
Rabbit Model ◽  
Factor Vii ◽  
Rabbit Brain ◽  
Atherosclerotic Plaques ◽  
E Coli ◽  
Amino Acid Levels ◽  
Human And Mouse

SummaryTissue factor (TF) is a membrane anchored glycoprotein that initiates blood coagulation by forming a complex with circulating factor VII or VIIa. TF has been identified in atherosclerotic plaques and may possibly trigger thrombosis after spontaneous plaque rupture as seen in acute myocardial infarction or angioplasty. We have previously developed an atherosclerotic rabbit model for study of the acute and chronic outcomes following angioplasty. As a first step in developing inhibitors of TF, we have isolated and characterized a rabbit cDNA coding for the mature TF. The sequence comparison of rabbit TF cDNA with those of human and mouse TFs show considerable similarity at both the nucleotide and amino acid levels. The TF cDNA when expressed in E. coli demonstrates a procoagulant activity comparable to that of native rabbit brain TF. The TF activity can be blocked by a polyclonal antibody against rabbit TF.

Differences in the chromatin structure and cis-element organization of the human and mouse GATA1 loci: implications for cis-element identification

Blood ◽  
2004 ◽  
Vol 104 (10) ◽  
pp. 3106-3116 ◽  
Author(s):  
Veronica Valverde-Garduno ◽  
Boris Guyot ◽  
Eduardo Anguita ◽  
Isla Hamlett ◽  
Catherine Porcher ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Sequence Comparison ◽  
Regulatory Element ◽  
Cis Elements ◽  
Enhancer Activity ◽  
Cis Element ◽  
Human Sequence ◽  
Human And Mouse

Abstract Cis-element identification is a prerequisite to understand transcriptional regulation of gene loci. From analysis of a limited number of conserved gene loci, sequence comparison has proved a robust and efficient way to locate cis-elements. Human and mouse GATA1 genes encode a critical hematopoietic transcription factor conserved in expression and function. Proper control of GATA1 transcription is critical in regulating myeloid lineage specification and maturation. Here, we compared sequence and systematically mapped position of DNase I hypersensitive sites, acetylation status of histone H3/H4, and in vivo binding of transcription factors over approximately 120 kilobases flanking the human GATA1 gene and the corresponding region in mice. Despite lying in approximately 10 megabase (Mb) conserved syntenic segment, the chromatin structures of the 2 homologous loci are strikingly different. The 2 previously unidentified hematopoietic cis-elements, one in each species, are not conserved in position and sequence and have enhancer activity in erythroid cells. In vivo, they both bind the transcription factors GATA1, SCL, LMO2, and Ldb1. More broadly, there are both species- and regulatory element–specific patterns of transcription factor binding. These findings suggest that some cis-elements regulating human and mouse GATA1 genes differ. More generally, mouse human sequence comparison may fail to identify all cis-elements.

Genomic sequence comparison of the human and mouse adenosine deaminase gene regions

1999 ◽  
Vol 10 (2) ◽  
pp. 95-101 ◽  
Author(s):  
Anthony G. Brickner ◽  
Ben F. Koop ◽  
Bruce J. Aronow ◽  
Dan A. Wiginton
Keyword(s):  
Adenosine Deaminase ◽  
Sequence Comparison ◽  
Genomic Sequence ◽  
Human And Mouse

scholarly journals DQX1, an RNA-dependent ATPase homolog with a novel DEAQ box: expression pattern and genomic sequence comparison of the human and mouse genes

2001 ◽  
Vol 12 (6) ◽  
pp. 456-461 ◽  
Author(s):  
Weizhen Ji ◽  
Feng Chen ◽  
Trang Do ◽  
Anh Do ◽  
Bruce A. Roe ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Sequence Comparison ◽  
Genomic Sequence ◽  
Dependent Atpase ◽  
Human And Mouse

Characterization of the human and mouse Fli-1 promoter regions

1996 ◽  
Vol 1307 (2) ◽  
pp. 220-232 ◽  
Author(s):  
Benoit Barbeau ◽  
Dominique Bergeron ◽  
Martin Beaulieu ◽  
Zahida Nadjem ◽  
Eric Rassart
Keyword(s):  
Promoter Regions ◽  
Human And Mouse

scholarly journals The Activity of Sendai Virus Genomic and Antigenomic Promoters Requires a Second Element Past the Leader Template Regions: a Motif (GNNNNN)3 Is Essential for Replication

1998 ◽  
Vol 72 (4) ◽  
pp. 3117-3128 ◽  
Author(s):  
Caroline Tapparel ◽  
Diane Maurice ◽  
Laurent Roux
Keyword(s):  
Sequence Comparison ◽  
Sendai Virus ◽  
Rna Replication ◽  
Negative Polarity ◽  
Relative Positioning ◽  
Promoter Regions ◽  
Complementary Sequence ◽  
Replication Activity ◽  
Repeated Motif ◽  
Selection Of

ABSTRACT The paramyxovirus genome, a nonsegmented, negative-polarity, single-stranded RNA of ∼15 kb, contains six transcription units flanked at the 3′ and 5′ ends by a short (∼ 50- to 60-nucleotide) extracistronic sequence, dubbed the positive and negative leader regions. These leader template regions, present at the 3′ end of the genome and the antigenome, have been shown to contain essential signals governing RNA replication activity. Whether they are sufficient to promote replication is still open to question. By using a series of Sendai virus defective interfering RNAs carrying a nested set of deletions in the promoter regions, it is shown here that for both the genomic and antigenomic promoters, a 3′-end RNA sequence of 96 nucleotides is required to allow replication. Sequence comparison of active and inactive promoters led to the identification of a set of three nucleotide hexamers (nucleotides 79 to 84, 85 to 90, and 91 to 96) containing a repeated motif RXXYXX [shown as 5′-3′ positive-strand]. Sequential mutation of each hexamer into its complementary sequence confirmed their essential role. The three hexamers are required, and their relative positioning is important, since displacing them by 6 nucleotides destroyed promoter function. RNAs carrying degenerate nucleotides in the three hexamers were used as replication templates. They led to the selection of actively replicating RNA species exclusively carrying the basic motif (GNNNNN)3 from nucleotides 79 to 96. These results clearly show that, apart from the region from nucleotides 1 to 31, previously identified as governing Sendai virus replication activity, a second element, spanning at the most nucleotides 79 to 96, appears essential. Thus, the paramyxovirus replication promoters are not confined to the leader template regions, as seems to be the case for the rhabdoviruses.

Genomic sequence comparison of the human and mouse XRCC1 DNA repair gene regions

Genomics ◽  
1995 ◽  
Vol 25 (2) ◽  
pp. 547-554 ◽  
Author(s):  
Jane E. Lamerdin ◽  
Mishelle A. Montgomery ◽  
Stephanie A. Stilwagen ◽  
Lisa K. Scheidecker ◽  
Robert S. Tebbs ◽  
...  
Keyword(s):  
Dna Repair ◽  
Sequence Comparison ◽  
Genomic Sequence ◽  
Repair Gene ◽  
Dna Repair Gene ◽  
Human And Mouse
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