Guanidinium isothiocyanate

doi:10.1101/pdb.caut416

Guanidiniumthiocyanate (GTC; guanidinium-isothiocyanate)

The Dictionary of Genomics, Transcriptomics and Proteomics ◽

10.1002/9783527678679.dg05301 ◽

2015 ◽

pp. 1-1

Keyword(s):

Guanidinium Isothiocyanate

Analysis of melanoma cells in peripheral blood by reverse transcription-polymerase chain reaction for tyrosinase and MART-1 after mononuclear cell collection with cell preparation tubes: a comparison with the whole blood guanidinium isothiocyanate RNA isolation method

Melanoma Research ◽

10.1097/00008390-200004000-00004 ◽

2000 ◽

Vol 10 (2) ◽

pp. 119-126 ◽

Cited By ~ 1

Author(s):

T. J. de Vries ◽

A. Fourkour ◽

C. J. A. Punt ◽

D. J. Ruiter ◽

G. N. P. van Muijen

Keyword(s):

Polymerase Chain Reaction ◽

Reverse Transcription ◽

Whole Blood ◽

Peripheral Blood ◽

Rna Isolation ◽

Cell Preparation ◽

Isolation Method ◽

Chain Reaction ◽

Guanidinium Isothiocyanate ◽

Polymerase Chain

Variasi Presipitasi Pelarut pada Ekstraksi RNA dengan Metode Trizole dan Pengaruh terhadap Kemurnian RNA

Media Kedokteran Hewan ◽

10.20473/mkh.v31i1.2020.33-44 ◽

2020 ◽

Vol 31 (1) ◽

pp. 33

Author(s):

Siti Shaomianah

Keyword(s):

Aedes Aegypti ◽

Dimethyl Sulfoxide ◽

Aedes Albopictus ◽

Dimethyl Formamide ◽

Guanidinium Isothiocyanate

ABSTRAKVirus dengue ditularkan oleh nyamuk Aedes aegypti dan Aedes albopictus. Nyamuk-nyamuk tersebut endemik di sebagian besar daerah vektor-vektor itu muncul memiliki empat serotipe yang berbeda secara antigenik. Empat serotipe virus dengue: DENV-1, DENV-2, DENV-3 dan DENV-4. Penelitian ini bertujuan untuk mengetahui perbandingan pelarut yang baik terhadap virus Dengue RNA Surabaya tipe-1 (DENV-1). Pelarut yang digunakan adalah Isopropanol, dimethyl sulfoxide, 96% ethanol, methanol, acetone-Methanol (1: 1), dimethyl formamide, dan akuades. RNA diisolasi dengan metode TRIzol. TRIzol (atau TRI Reagent) adalah larutan satu fasa dari fenol dan guanidinium isothiocyanate yang secara bersamaan melarutkan bahan biologis dan mendenaturasi protein. Hasil dari penilitian ini adalah didapatkan pelarut presipitasi RNA terbaik adalah aseton-metanol (1:1), karena campuran pelarut tersebut memiliki konstanta dielektrik terendah. Studi ini menunjukkan bahwa pelarut aseton-metanol (1:1) dapat digunakan sebagai pelarut presipitasi untuk mengisolasi RNA

An efficient guanidinium isothiocyanate additive for improving the photovoltaic performances and thermal stability of perovskite solar cells

Electrochimica Acta ◽

10.1016/j.electacta.2018.08.117 ◽

2018 ◽

Vol 291 ◽

pp. 297-303 ◽

Cited By ~ 8

Author(s):

Junjie Zou ◽

Weier Liu ◽

Wenqiu Deng ◽

Gui Lei ◽

Shi Zeng ◽

...

Keyword(s):

Thermal Stability ◽

Solar Cells ◽

Perovskite Solar Cells ◽

Guanidinium Isothiocyanate ◽

Thermal Stability Of

Novel use of guanidinium isothiocyanate in the isolation ofMycobacterium tuberculosisDNA from clinical material

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.2001.tb10933.x ◽

2001 ◽

Vol 205 (1) ◽

pp. 113-117 ◽

Cited By ~ 14

Author(s):

Soumitesh Chakravorty ◽

Jaya S Tyagi

Keyword(s):

Clinical Material ◽

Guanidinium Isothiocyanate

Analysis of melanoma cells in peripheral blood by reverse transcription-polymerase chain reaction for tyrosinase and MART-1 after mononuclear cell collection with cell preparation tubes: a comparison with the whole blood guanidinium isothiocyanate RNA isolation method

Melanoma Research ◽

10.1097/00008390-200010020-00004 ◽

2000 ◽

Vol 10 (2) ◽

pp. 119-126 ◽

Cited By ~ 9

Author(s):

T. J. de Vries ◽

A. Fourkour ◽

C. J. A. Punt ◽

D. J. Ruiter ◽

G. N. P. van Muijen

Keyword(s):

Polymerase Chain Reaction ◽

Reverse Transcription ◽

Whole Blood ◽

Peripheral Blood ◽

Rna Isolation ◽

Cell Preparation ◽

Isolation Method ◽

Chain Reaction ◽

Guanidinium Isothiocyanate ◽

Polymerase Chain

DNA binding proteins present in guanidinium isothiocyanate lysates of cells are suitable for specific binding site blotting

Nucleic Acids Research ◽

10.1093/nar/17.21.8891 ◽

1989 ◽

Vol 17 (21) ◽

pp. 8891-8891 ◽

Cited By ~ 1

Author(s):

Murkus P. Freyaldenhoven ◽

Brigtte Royer-Pokra ◽

Inge Napierski ◽

Hans-Dieter Royer

Keyword(s):

Dna Binding ◽

Binding Site ◽

Binding Proteins ◽

Specific Binding ◽

Dna Binding Proteins ◽

Guanidinium Isothiocyanate

Encyclopedia of Genetics, Genomics, Proteomics and Informatics ◽

10.1007/978-1-4020-6754-9_7210 ◽

2008 ◽

pp. 829-829

Keyword(s):

Guanidinium Isothiocyanate

Preparation of RNA from Plant Tissue Using Guanidinium Isothiocyanate/Cesium Chloride Ultracentrifugation

Cold Spring Harbor Protocols ◽

10.1101/pdb.prot4102 ◽

2006 ◽

Vol 2006 (1) ◽

pp. pdb.prot4102

Author(s):

Michael A. Connolly ◽

Peter A. Clausen ◽

James G. Lazar

Keyword(s):

Plant Tissue ◽

Cesium Chloride ◽

Guanidinium Isothiocyanate

Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR

Frontiers in Genetics ◽

10.3389/fgene.2021.632232 ◽

2021 ◽

Vol 12 ◽

Author(s):

Dongyong Lv ◽

Yaohua Fan ◽

Wanhua Zhong ◽

Piyanuch Lonan ◽

Kunfeng Liu ◽

...

Keyword(s):

Genetic Information ◽

Phylogenetic Trees ◽

Sodium Dodecyl ◽

Genetic Identification ◽

Gene Sequences ◽

Simple Method ◽

Guanidinium Isothiocyanate ◽

Arms Pcr ◽

Edible Bird’S Nest ◽

Nd2 Gene

Edible bird’s nest (EBN) is a popular delicacy in the Asian Pacific region originating from Indonesia, Malaysia, Thailand and Vietnam, which consist of various potential medicine value in Traditional Chinese Medicine (TCM). Thailand is one of the main exporters of EBN. However, the genetic information of EBN, a key part of molecular biology, has yet to be reported in Thailand. It is necessary to explore the genetic information of EBN in Thailand based on a quick and simple method to help protect the rights and interests of consumers. This research aimed to systematically evaluate different methods of extracting EBN DNA to improve the efficiency of the analysis of cytochrome b (Cytb) and NADH dehydrogenase subunit 2 (ND2) gene sequences, the establishment of phylogenetic trees, and the genetic information of EBN in Thailand. Additionally, we aimed to develop a quick and simple method for identifying EBN from different species based on the genetic information and amplification-refractory mutation system PCR (ARMS-PCR). By comparing the four methods [cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), kit and guanidinium isothiocyanate methods] for EBN extraction, we found that the guanidinium isothiocyanate method was the optimal extraction method. Phylogenetic trees generated on the basis of Cytb and ND2 gene analyses showed that 26 samples of house EBN and 4 samples of cave EBN came from Aerodramus fuciphagus and Aerodramus maximus, respectively. In addition, to distinguish different samples from different species of Apodiformes, we designed 4 polymerase chain reaction (PCR) amplification primers based on the ND2 gene sequences of A. fuciphagus and A. maximus. The ARMS-PCR results showed band lengths for A. fuciphagus EBN of 533, 402, and 201 bp, while those for A. maximus EBN were 463, 317, and 201 bp. Collectively, the results showed that ARMS-PCR is a fast and simple method for the genetic identification of EBN based on designing specific original identification primers.